检索结果-南通市图书馆

Insect Science 2022年第3期29卷 840-854页

作者： Hai-Zhong Yu Qin zhang zhan-jun lu Ming-Jie Deng College of Life Sciences Gannan Normal UniversityGanzhouJiangxi ProvinceChina National Navel Orange Engineering Research Center GanzhouJiangxi ProvinceChina Analytical and Testing Center School of Pharmaceutical SciencesWenzhou Medical UniversityWenzhouZhejiang ProvinceChina

Validamycin,as a broadly applied antibiotic,has been used to control rice sheath blight ***,validamycin was considered as an insecticide to control agricultural *** into the mechanism of validamycin’s action on insects can provide molecular targets for the control of agricultural *** this study,a toxicological test analysis revealed that Spodoptera litura larval growth and development was significantly inhibited and the pupation rate was significantly reduced with the increase of the concentration of *** to the NMR-based metabolomic analysis,a total of 15 metabolites involved in glycolysis and tricarboxylic acid cycle(TCA)pathways were ***,trehalase activities,glucose and chitin contents were significantly downregulated,but the trehalose content was upregulated after exposure to *** transcription quantitative PCR analysis revealed that the expression level of genes involved in glycolysis,TCA and chitin synthesis were upregulated after treating with *** chitin staining also confirmed that chitin content was downregulated at 12 h after validamycin *** results indicated that validamycin worked via two different molecular mechanisms,one through inhibiting glycometabolism and the other by inhibiting chitin synthesis in *** information lays a theoretical foundation for further control of ***.

关键词： chitin synthesis glycometabolism ^(1)H-NMR metabonomics Spodoptera litura validamycin

Protective effects of a composition of Chinese herbs- Gurigumu-13 on retinal ganglion cell apoptosis in DBA/2J glaucoma mouse model

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2018年第3期11卷 363-368页

作者： Qiu-Li zhang Wei Wang Yan Jiang Tian-Zi zhang zhan-jun lu Ao Jiang Department of Ophthalmology Affiliated Hospital of Inner Mongolia University for the Nationalities Tongliao 028000 the Inner Mongolia Autonomous Region China Department of Ophthalmology Tongliao Hospital Tongliao 028000 the Inner Mongolia Autonomous Region China Department of Neurosurgery Affiliated Hospital of Inner Mongolia University for the Nationalities Tongliao 028000 the Inner Mongolia Autonomous Region China China-Japan Union Hospital of Jilin University Changchun 130000 Jilin Province China

AIM： To explore the concrete mechanism of a Mongolian compound medicine-Gurigumu-13 （GRGM） for glaucoma treatment. METHODS： DBA/2J mice, as glaucoma models, were intragastric administrated with GRGM to study the effect of GRGM on retinal ganglion cells （RGCs）. The loss of RGCs was evaluated with the number of RGCs and axons. The expression of the target protein of RGCs or mouse retinas was determined by Western blot. The relative content of malondialdehyde （MDA） was examined by ELISA assay. RESULTS： GRGM distinctly improved retina damage via increasing the number of neurons, RGCs and axons in a concentration dependent manner. Meanwhile, GRGM obviously decreased the high level of MDA and the expression of oxidative stress-related proteins in retinas of DBAJ2J mice, but promoted the expression of antioxidant proteins. Additionally, GRGM also significantly inhibited the protein expression of Bip and Chop, which were markers of endoplasmic reticulum stress-induced apoptosis. CONCluSION： GRGM have obvious protective effects on RGCs in DBA/2J mice, and increase the number of RGCs and axons via inhibiting oxidative stress and endoplasmic reticulum stress.

关键词： glaucoma retinal ganglion cells oxidativestress endoplasmic reticulum stress

MicroRNA-155 promotes the pathogenesis of experimental colitis by repressing SHIP-1 expression

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2017年第6期23卷 976-985页

作者： zhan-jun lu Jian-Jiong Wu Wei-Liang Jiang jun-Hua Xiao Kai-Zhong Tao Lei Ma Ping Zheng Rong Wan Xing-Peng Wang Department of Gastroenterology Shanghai General HospitalShanghai Jiao Tong University School of Medicine Department of Gastroenterology Shanghai East HospitalSchool of MedicineTong Ji University

AIM To explore the mechanism by which microRNA-155 (miR-155) regulates the pathogenesis of experimental colitis. METHODS A luciferase assay was performed to confirm the binding of miR-155 to the SHIP-1 3'-UTR. MiR-155 mimics, negative controls and SHIP-1 expression/knockdown vectors were established and then utilized in gain-and loss-of-function studies performed in raw264.7 cells and primary bone marrow-derived macrophages (BMDMs). Thereafter, dextran sulfate sodium (DSS)-induced colitis mouse model with or without antagomiR-155 treatment was established, and the levels of miR-155 and SHIP-1, as well as the pro-inflammatory capabilities, were measured by western blot, quantitative polymerase chain reaction, and immunohistochemistry. RESULTS MiR-155 directly bound to the 3'-UTR of SHIP-1 mRNA and induced a significant decrease in SHIP-1 expression in both raw264.7 cells and primary BMDMs. MiR-155 markedly promoted cell proliferation and proinflammatory secretions including IL-6, TNF-alpha, IL-1 beta, and IFN-gamma, whereas these effects could be reversed by the restoration of SHIP-1 expression. In vivo studies showed that antagomiR-155 administration could alleviate DSS-induced intestinal inflammation in Balb/c mice. Moreover, significantly increased SHIP-1 expression, as well as decreased Akt activation and in-flammatory response, were observed in the antagomiR-155-treated mice. CONCluSION MiR-155 promotes experimental colitis by repressing SHIP-1 expression. Thus, the inhibition of miR-155 might be a promising strategy for therapy.

关键词： Experimental colitis Inflammatory bowel disease MicroRNA-155 SHIP-1

Inhibition of trehalase affects the trehalose and chitin metabolism pathways in Diaphorina citri(Hemiptera:Psyllidae)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2021年第3期28卷 718-734页

作者： Hai-Zhong Yu Yu-Ling Huang zhan-jun lu Qin zhang Hua-Nan Su Yi-Ming Du Long Yi Ba-Lian Zhong Ci-Xiang Chen College of Life Sciences Gannan Normal UniversityGanzhouJiangxiChina National Navel Orange Engineering Research Center GanzhouJiangxiChina China-USA Citrus Huanglongbing Joint Laboratory A Joint Laboratory of The University of Florida and Gannan Normal UniversityGanzhouJiangxiChina Fruit Bureau of Ganzhou GanzhouJiangxiChina

The Asian citrus psyllid,Diaphorina citri is the principal vector of huanglongbing,which transmits Candidatus Liberibacter *** is a key enzyme involved in trehalose hydrolysis and plays an important role in insect growth and *** specific functions of this enzyme in *** have not been *** this study,three trehalase genes(DcTreJ-1,DcTrel-2,and DcTre2)were identified based on the *** genome *** analysis showed that DcTrel-1 and DcTrel-2 are related to soluble trehalase,whereas DcTre2 is associated with membrane-bound *** expression analysis indicated that DcTrel-1 and DcTrel-2 had the highest expression levels in the head and wing,respectively,and DcTre2 had high expression levels in the fat ***,DcTrel-1 and DcTrel-2 expression levels were induced by 20-hydroxyecdysone and juvenile hormone III,but DcTre2 was *** expression levels of DcTrel-1,DcTrel-2,and DcTre2 were significantly up-regulated,which resulted in high mortality after treatment with *** activities and glucose contents were downregulated,but the trehalose content increased after treatment with *** addition,the expression levels of chitin metabolism-related genes significantly decreased at 24 and 48 h after treatment with ***,silencing of DcTrel-1,DcTrel-2,and DcTre2 reduced the expression levels of chitin metabolism-related genes and led to a malformed phenotype of *** results indicate that *** trehalase plays an essential role in regulating chitin metabolism and provides a new target for control of ***.

关键词： chitin metabolism Diaphorina citric RNA interference trehalase validamycin

Integrated transcriptome sequencing and RNA interference reveals molecular changes in Diaphorina citri after exposure to validamycin

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2021年第6期28卷 1690-1707页

作者： Hai-Zhong Yu Yan-Xin Xie Jie Wang Ying Wang Yi-Min Du He-Gui Wang Ba-Lian Zhong Bo Zhu Xiu-Dao Yu zhan-jun lu College of Life Sciences Gannan Normal UniversityGanzhouJiangxi ProvinceChina National Navel Orange Engineering Research Center GanzhouJiangxi ProvinceChina College of Life Science Anhui Agricultural UniversityHefeiChina

Validamycin has been widely used as a specific competitive inhibitor of *** our previous research,validamycin significantly inhibited trehalase activity and chitin synthesis in Diaphorina citri,resulting in abnormal ***,the mechanism of validamycin's action on *** remains ***,using a comparative transcriptome analysis,464 differentially expressed genes(DEGs)in *** were identified after validamycin treatment.A Gene Ontology enrichment analysis revealed that these DEGs were mainly involved in“small molecule process”,“structural molecule ac-tivity”and“transition metal ion binding”.DEGs involved in chitin metabolism,cuticle synthesis and insecticide detoxification were validated by reverse transcription quantitative polymerase chain *** RNA interference of *** chitinase-like protein EN03 and *** cuticle protein 7 genes significantly affected *** ***,the recombinant chitinase-like protein EN03 exhibited a chitin-binding property,and an antimicrobial activity against Bacillus *** study provides a first insight into the molecular changes in *** after exposure to validamycin and identifies two effective RNA interference targets for *** control.

关键词： chitin-binding Diaphorina citric RNA interference transcriptome sequencing validamycin

Inhibitory effects of safranal on laser-induced choroidal neovascularization and human choroidal microvascular endothelial cells and related pathways analyzed with transcriptome sequencing

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2021年第7期14卷 981-989页

作者： Qin-Xiao Yao-Yao Sun zhan-jun lu Tian-Zi zhang Shan-Shan Li Ting Hua Suriguga Wen-Lin Chen Lin-Lin Ran Wen-Zhen Yu Fei Yang Burenbatu Department of Ophthalmology Affiliated Hospital of Inner Mongolia University for NationalitiesTongliao 028007Inner Mongolia Autonomous RegionChina Department of Ophthalmology Peking University People’s HospitalBeijing 100044China College of Mongolian Medicine Inner Mongolia University for NationalitiesTongliao 028000Inner Mongolia Autonomous RegionChina Department of Ophthalmology Peking University International HospitalBeijing 100026China Department of Hematology Affiliated Hospital of Inner Mongolia University for NationalitiesTongliao 028007Inner Mongolia Autonomous RegionChina

AIM:To determine the effects of safranal on choroidal neovascularization(CNV)and oxidative stress damage of human choroidal microvascular endothelial cells(HCVECs)and its possible ***:Forty-five rats were used as a laser-induced CNV model for testing the efficacy and safety of safranal(0.5 mg/kg·d,intraperitoneally)on *** leakage on fluorescein angiography(FA)and CNV thickness on histology was *** were used for a H_(2)O_(2)-induced oxidative stress model to test the effect of safranal in *** essay was carried to test the inhibition rate of safranal on cell viability at different *** formation was used to test protective effect of safranal on angiogenesis at different *** transcriptome sequencing was performed to find the possible signal *** expressions of different molecules and their phosphorylation level were validated by Western ***:On FA,the average CNV leakage area was 0.73±0.49 and 0.31±0.11 mm^(2)(P=0.012)in the control and safranal-treated group *** average CNV thickness was 127.4±18.75 and 100.6±17.34μm(P=0.001)in control and safranal-treated *** the condition of oxidative stress,cell proliferation was inhibited by safranal and inhibition rates were 7.4%-35.4%at the different *** tube formation study,the number of new branches was 364 in control group and 35,42,and 17 in 20,40,and 80μg/mL safranal groups respectively(P<0.01).From the KEGG pathway bubble graph,the PI3K-AKT signaling pathway showed a high gene *** protein expression was elevated of insulin receptor substrate(IRS)and the phosphorylation level of PI3K,phosphoinositide-dependent protein kinase 1/2(PDK1/2),AKT and Bcl-2 associated death promoter(BAD)was also elevated under oxidative stress condition but inhibited by ***:Safranal can inhibit CNV both in vivo and in vitro,and the IRS-PI3K-PDK1/2-AKT-BAD signaling pathway is involved in the pathogenesis

关键词： choroidal neovascularization safranal human choroidal microvascular endothelial cells oxidative stress transcriptomics

KMT2D deficiency enhances the anti-cancer activity of L48H37 in pancreatic ductal adenocarcinoma

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Oncology 2019年第8期11卷 599-621页

作者： Si-Si Li Wei-Liang Jiang Wen-Qin Xiao Kai Li Ye-Fei zhang Xing-Ya Guo Yi-Qi Dai Qiu-Yan Zhao Ming-Jie Jiang zhan-jun lu Rong Wan Department of Gastroenterology Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 201620China Shanghai Key Laboratory of Pancreatic Diseases Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 201620China

BACKGROUND Novel therapeutic strategies are urgently needed for patients with a delayed diagnosis of pancreatic ductal adenocarcinoma(PDAC)in order to improve their chances of *** studies have shown potent anti-neoplastic effects of curcumin and its *** addition,the role of histone methyltransferases on cancer therapeutics has also been ***,the relationship between these two factors in the treatment of pancreatic cancer remains *** working hypothesis was that L48H37,a novel curcumin analog,has better efficacy in pancreatic cancer cell growth inhibition in the absence of histonelysine N-methyltransferase 2D(KMT2D).AIM To determine the anti-cancer effects of L48H37 in PDAC,and the role of KMT2D on its therapeutic *** The viability and proliferation of primary(PANC-1 and MIA PaCa-2)and metastatic(SW1990 and ASPC-1)PDAC cell lines treated with L48H37 was determined by CCK8 and colony formation ***,mitochondrial membrane potential(MMP),reactive oxygen species(ROS)levels,and cell cycle profile were determined by staining the cells with Annexin-V/7-AAD,JC-1,DCFH-DA,and PI respectively,as well as flow cytometric *** vitro migration was assessed by the wound healing *** protein and mRNA levels of relevant factors were analyzed using Western blotting,immunofluorescence and real time-quantitative *** in situ expression of KMT2D in both human PDAC and paired adjacent normal tissues was determined by *** vivo tumor xenografts were established by injecting nude mice with PDAC *** analyses were also conducted using gene expression databases and *** L48H37 inhibited the proliferation and induced apoptosis in SW1990 and ASPC-1 cells in a dose-and time-dependent manner,while also reducing MMP,increasing ROS levels,arresting cell cycle at the G2/M stages and activating the endoplasmic reticulum(ER)stress-associated protein kinase RNA-like endoplasmic

关键词： Pancreatic neoplasms Curcumin analog Histone methyltransferase 2D Therapeutic effects Bioinformatics

Revolutionizing breast cancer detection:Advances in fluorescent probe technology

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2025年第1期18卷 653-674页

作者： Qian Gao Lin Tang jun zhan Yiyuan Xue Feng luo State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases West China Hospital of StomatologySichuan UniversityChengdu 610041China Emergency Obstetrics and Gynecology West China Second University HospitalSichuan UniversityChengdu 610041China Department of Obstetrics and Gynecology West China Second University HospitalSichuan UniversityChengdu 610041China Department of Prosthodontics West China School of StomatologySichuan UniversityChengdu 610041China

With the increasing incidence of breast cancer globally,there is an urgent demand for enhanced diagnostic and therapeutic *** comprehensive review explores advancements in fluorescent probe technology for breast cancer imaging and early *** probes offer unparalleled sensitivity and specificity in detecting cancer biomarkers,enabling early diagnosis and delineation of tumor margins during *** categorize these probes into molecular,nano,ratiometric,the second near-infrared window(NIR-II),and multifunctional varieties,each with unique capabilities for targeting specific cancer *** review highlights recent innovations in probe design,emphasizing their applications in pathogenesis studies,drug development,precision medicine,and fluorescence-guided *** their promising clinical potential,challenges such as safety,biomarker targeting accuracy,system compatibility,and clinical translation *** these hurdles is crucial for the integration of fluorescent probes into standard cancer care,aiming to improve treatment outcomes and patient quality of life.

关键词： breast cancer fluorescent probes imaging early diagnosis biomarker

From waste to wealth:Coal tar residue derived carbon materials as low-cost anodes for potassium-ion batteries

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 464-475页

作者： Zhonghua lu jun Shen Xin zhang Lingcong Chao Liang Chen Ding zhang Tao Wei Shoudong Xu College of Chemical Engineering and Technology Taiyuan University of TechnologyTaiyuan 030024China College of Chemistry Taiyuan University of TechnologyTaiyuan 030024China School of Chemical Engineering and Pharmacy Wuhan Institute of TechnologyWuhan 430205China School of Energy and Power Jiangsu University of Science and TechnologyZhenjiang 212003China

Carbon materials are widely recognized as highly promising electrode materials for various energy storage system *** tar residues(CTR),as a type of carbon-rich solid waste with high value-added utilization,are crucially important for the development of a more sustainable *** this study,we employed a straightforward direct carbonization method within the temperature range of 700-1000℃to convert the worthless solid waste CTR into economically valuable carbon materials as anodes for potassium-ion batteries(PIBs).The effect of carbonization temperature on the microstructure and the potassium ions storage properties of CTR-derived carbons(CTRCs)were systematically explored by structural and morphological characterization,alongside electrochemical performances *** on the co-regulation between the turbine layers,crystal structure,pore structure,functional groups,and electrical conductivity of CTR-derived carbon carbonized at 900℃(CTRC-900H),the electrode material with high reversible capacity of 265.6m Ah·g^(-1)at 50 m A·g^(-1),a desirable cycling stability with 93.8%capacity retention even after 100 cycles,and the remarkable rate performance for PIBs were ***,cyclic voltammetry(CV)at different scan rates and galvanostatic intermittent titration technique(GITT)have been employed to explore the potassium ions storage mechanism and electrochemical kinetics of *** indicate that the electrode behavior is predominantly governed by surface-induced capacitive processes,particularly under high current densities,with the potassium storage mechanism characterized by an“adsorption-weak intercalation”*** work highlights the potential of CTR-based carbon as a promising electrode material category suitable for high-performance PIBs electrodes,while also provides valuable insights into the new avenues for the high value-added utilization of CTR.

关键词： coal tar residue carbon materials anode potassium-ion batteries high value-added

Based on non-targeted metabolomics for differential components screening of Rosae Chinensis Flos and Rosae Rugosae Flos and their quality evaluation

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Traditional Medicine Research 2025年第2期10卷 1-15页

作者： Xu Liang Ni-Hui zhang Zhi-Lai zhan Guang-lu Chang Yan Gao Xia Li Wen-Yuan Gao School of Pharmaceutical Science and Technology Faculty of MedicineTianjin UniversityTianjin 300193China State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs China Academy of Chinese Medical SciencesBeijing 100700China Key Laboratory of Modern Chinese Medicine Resources Research Enterprises Tianjin 300402China

Background:Rosa chinensis *** Rosa rugosa *** not only of ornamental value,but also edible flowers and the flower buds have been listed in the Chinese Pharmacopoeia as traditional *** two plants have some differences in efficacy,but the flower buds are easily confused for similar *** addition,large-scale cultivation of ornamental rose flowers may lead to a decrease in the effective components of medicinal ***,it is necessary to study the chemical composition and make quality evaluation of Rosae Chinensis Flos(Yueji)and Rosae Rugosae Flos(Meigui).Methods:In this study,40 batches of samples including Meigui and Yueji from different regions in China were collected to establish high-performance liquid chromatography ***,the fingerprints data was analyzed using principal component analysis,hierarchical cluster analysis,and partial least squares discriminant analysis analysis chemometrics to obtain information on intergroup differences,and non-targeted metabolomic techniques were applied to identify and compare chemical compositions of samples which were chosen from groups with large *** compounds were screened by orthogonal partial least-squares discriminant analysis and S-plot,and finally multi-component quantification was performed to comprehensively evaluate the quality of Yueji and ***:The similarity between the fingerprints of 40 batches roses and the reference print R was 0.73 to 0.93,indicating that there were similarities and differences between the *** principal component analysis and hierarchical cluster analysis of fingerprints data,the samples from different origins and varieties were intuitively divided into four *** least-squares discriminant analysis analysis showed that Meigui and Yueji cluster into two categories and the model was reliable.A total of 89 compounds were identified by high resolution mass spectrometry,mainly were flavonoids and flavon

关键词： Rosa chinensis Jacq. Rosa rugosa Thunb. metabolomics chemometrics multiple component quantification quality evaluation