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Improved Differential Evolution Algorithm and its Application in Complex Function Optimization

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Improved Differential Evolution Algorithm and its Applicatio...

Analytical validation of GMEX rapid point- of care CYP2C19 genotyping system for the CHAnCE-2 trial

第26届中国控制与决策会议

作者： XiaoGang dong Yan Liu ChangShou Deng School of Information Science and Technology Jiujiang University

When solving complex function optimization problem, Differential evolution（DE） algorithms may suffer from low convergence rate. In this paper, we propose an improved differential evolution algorithm named n-IDE. Our algorithm uses Gaussian sequence to dynamically generate zoom factors and applies an improved hybrid mutation strategy to individuals in order to improve the overall performance. We compare n-IDE with existing DE approaches using benchmark functions and the experimental result shows that n-IDE has significant improvement on the convergence rate.

关键词： Function Optimization Differential evolution Hybrid Mutation Gaussian sequence

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Stroke & Vascular neurology 2021年第2期6卷 274-279页

作者： Xia Meng Anxin Wang Guojun zhang Siying niu Wei Li Sifei Han Fang Fang Xingquan zhao Kehui dong Zening Jin Huaguang zheng Kelin Chen Hao Li Chengyuan Yang Yongjun Wang China national Clinical Research Center for neurological Diseases Beijing Tiantan HospitalCapital Medical UniversityBeijingChina Department of neurology Beijing Tiantan HospitalCapital Medical UniversityBeijingChina Department of Clinical Diagnosis Laboratory Beijing Tiantan HospitalCapital Medical UniversityBeijingChina Biomedical and Health Informatics The Children's Hospital of PhiladelphiaPhiladelphiaPennsylvaniaUSA Department of Cardiology Beijing Tiantan HospitalCapital Medical UniversityBeijingChina

Background and purpose Rapid genotyping is useful for guiding early antiplatelet therapy in patients with high-risk nondisabling ischaemic cerebrovascular events(HR-nICE).Conventional genetic testing methods used in CYP2C19 genotype-guided antiplatelet therapy for patients with HR-nICE did not satisfy the needs of the Clopidogrel in High-Risk Patients with Acute nondisabling Cerebrovascular Events(CHAnCE)-2 ***,we developed the rapid-genotyping GMEX(point-of care)system to meet the needs of the CHAnCE-2 *** Healthy individuals and patients with history of cardiovascular diseases(n=408)were enrolled from six centres of the CHAnCE-2 *** compared the laboratory-based genomic test results with Sanger sequencing test results for accuracy ***,we demonstrated the accuracy,timeliness and clinical operability of the GMEX system compared with laboratory-based technology(YZY Kit)to verify whether the GMEX system satisfies the needs of the CHAnCE-2 *** Genotypes reported by the GMEX system showed 100%agreement with those determined by using the YZY Kit and Sanger sequencing for all three CYP2C19 alleles(*2,*3 and*17)*** average result’s turnaround times for the GMEX and YZY Kit methods were 85.0(IQR:85.0-86.0)and 1630.0(IQR:354.0-7594.0)min(pnd feasible point-of care system for rapid CYP2C19 genotyping for the CHAnCE-2 trial or related clinical and research applications.

关键词： CYP2C19 Acute system

Breast Cancer Resistance Protein Expression and 5-Fluorouracil Resistance

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Biomedical and Environmental Sciences 2008年第4期21卷 290-295页

作者： JIAn-HUI YUAn JIn-QUAn CHEnG LOnG-YUAn JIAnG WEI-dong JI LIAnG-FEnG GUO JIAn-JUn LIU XInG-YUn XU JInG-SOnG HE XIAn-MInG WAnG zhI-XIOnG zhUAnG Faculty of Preventive Medicine School of Public Health Sun Yat-Sen University Guangzhou 510080Guangdong China Toxicology Laboratory Municipal Center for Disease Control and PreventionShenzhen 518020 Guangdong China Department of Emergency The Second Affiliated Hospital of Sun Yat-Sen University Guangzhou 510120 Guangdong China Department of General Surgery Shenzhen Second People＇s Hospital Shenzhen 518035 Guangdong China

Objective To filtrate breast cancer resistance protein （BCRP）-mediated resistant agents and to investigate clinical relationship between BCRP expression and drug resistance. Methods MTT assay was performed to filtrate BCRP-mediated resistant agents with BCRP expression cell model and to detect chemosensitivity of breast cancer tissue specimens to these agents. A high performance liquid chromatography （HPLC） assay was established, and was used to measure the relative dose of intracellular retention resistant agents. RT-PCR and immunohistochemistry （IHC） were employed to investigate the BCRP expression in breast cancer tissue specimens. Results MTT assay showed that the expression of BCRP increased with the increasing resistance of 5-fluorouracil （5-Fu）（P〈0.05, n=3） in the cell model, while HPLC assay indicated that the intracellular retention dose of 5-Fu was significantly correlated with the expression of BCRP （t=-0.897, P〈0.05, n=3）. A total of 140 breast cancer tissue specimens were collected. BCRP-positive expression was detected in forty-seven specimens by both RT-PCR and IHC. As shown by MTT assay subsequently, the resistance index （RI） of 47 BCRP-positive breast cancer tissue specimens to 5-Fu was 7-12 times as high as that of adjacent normal tissue samples. BCRP expression was related to 5-Fu resistance （R2=0.8124, P〈0.01）. Conclusion Resistance to 5-Fu can be mediated by BCRR Clinical chemotherapy for breast cancer patients can be optimized based on BCRP-positive expression.

关键词： Breast cancer resistance protein 5-fluorouracil Breast cancer Resistance Chemotherapy

Anti-Helicobacter pylori therapy followed by celecoxib on progression of gastric precancerous lesions

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World Journal of Gastroenterology 2009年第22期15卷 2731-2738页

作者： Li-Jing zhang Shi-Yan Wang Xiao-Hui Huo zhen-Long zhu Jian-Kun Chu Jin-Cheng Ma dong-Sheng Cui Ping Gu Zeng-Ren zhao Ming-Wei Wang Jun Yu Department of Gastroenterology the First Affiliated Hospital of Hebei Medical University Shijiazhuang 050051 Hebei Province China Department of Medicine and Therapeutics the Prince of Wales Hospital the Chinese University of Hong Kong Hong Kong China Department of Pathology the First Affiliated Hospital of Hebei Medical University Shijiazhuang 050051 Hebei Province China Department of neurology the First Affiliated Hospital of Hebei Medical University Shijiazhuang 050051 Hebei Province China Department of Surgery the First Affiliated Hospital of Hebei Medical University Shijiazhuang 050051 Hebei Province China

AIM： To evaluate whether celecoxib, a selective cyclooxygenase 2 （COX-2） inhibitor, could reduce the severity of gastric precancerous lesions following Hel/cobacter pylori （H pylorl） eradication. METHODS： H pylori-eradicated patients with gastric precancerous lesions randomly received either celecoxib （n = 30） or placebo （n = 30） for up to 3 mo. COX-2 expression and activity was determined by immunostaining and prostaglandin E2 （PGE2） assay, cell proliferation by Ki-67 immunostaining, apoptosis by TUnEL staining and angiogenesis by microvascular density （MVD） assay using CD31 ***： COX-2 protein expression was significantly increased in gastric precancerous lesions （atrophy, intestinal metaplasia and dysplasia, respectively） compared with chronic gastritis, and was concomitant with an increase in cell proliferation and angiogenesis. A significant improvement in precancerous lesions was observed in patients who received celecoxib compared with those who received placebo （P 〈 0.001）. Of these three changes, 84.6% of sites with dysplasia regressed in patients treated with celecoxib （P = 0.002） compared with 60% in the placebo group, suggesting that celecoxib was effective on the regression of dysplasia. COX-2 protein expression （P 〈 0.001） and COX-2 activity （P 〈 0.001） in the gastric tissues were consistently lower in celecoxib-treated patients compared with the placebo-treated subjects. Moreover, it was also shown that celecoxib suppressed cell proliferation （P 〈 0.01）, induced cell apoptosis （P 〈 0.01） and inhibited angiogenesis with decreased MVD （P 〈 0.001）. However, all of these effects were not seen in placebo-treated subjects. Furthermore, COX-2 inhibition resulted in the up-regulation of PPARy expression, a protective molecule with anti-neoplastic effects. COnCLUSIOn： H pylori eradication therapy followed by celecoxib treatment improves gastric precancerous lesions by inhibiting COX-2 activity, inducing apoptosis, and suppressing cell proliferation

关键词： Apoptosis Cyclooxygenase 2 Gastric precancerous lesions Helicobacter pylori Microvessel density Proliferation

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利用整合型人工湿地处理水质污染物研究

生态经济 2015年第5期31卷 26-28,44页

作者：董禹北京大学光华管理学院北京100871 中国国际经济咨询有限公司北京100004

系统介绍了整合型人工湿地（ICW）系统的发展、特点、优势和局限性。另外,此研究还以位于爱尔兰北部格拉斯洛赫地区占地32 500 m2的整合型人工湿地系统为例,讨论水力负荷和季节性气温变化对系统污染物净化效果的影响。通过对2010~2013... 详细信息

系统介绍了整合型人工湿地（ICW）系统的发展、特点、优势和局限性。另外,此研究还以位于爱尔兰北部格拉斯洛赫地区占地32 500 m2的整合型人工湿地系统为例,讨论水力负荷和季节性气温变化对系统污染物净化效果的影响。通过对2010~2013年的监测数据分析发现,所研究的ICW系统对化学需氧量（COD）、生化需氧量（BOD）、总悬浮固体量（TSS）、氨氮（nH3-n）、硝基氮（nO3-n）、总氮量（Tn）、钼酸盐反应磷（MRP）的净化能力分别为：92%、98%、96%、97%、89%、96%、95%。经计算,ICW系统的污染物净化能力不受水力负荷波动影响。除此之外,低温会降低整合型人工湿地中微生物的活性,从而影响ICW系统的整体处理效果。

关键词：湿地除氮除磷水力负荷

Soluble expression and characterization of a GFP-fused pea actin isoform (PEAc1)

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Cell Research 2004年第5期14卷 407-414页

作者： Ai Xiao LIU*, Shao Bin zhAnG*, Xiao Jing XU, dong Tao REn, Guo Qin LIU** State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Science, China Agricultural University, Beijing 100094, China. State Key Laboratory of Plant Physiology and Biochemistry College of Biological Science China Agricultural University Beijing 100094 China

A pea actin isoform PEAc1 with green fluorescent protein (GFP) fusion to its C-terminus and His-tag to its n- terminus, was expressed in prokaryotic cells in soluble form, and highly purified with ni-Chelating SepharoseTM Fast Flow column. The purified fusion protein (PEAc1-GFP) efficiently inhibited Dnase I activities before polymerization, and activated the myosin Mg-ATPase activities after polymerization. The PEAc1-GFP also polymerized into green fluorescent filamentous structures with a critical concentration of 0.75 μM. These filamentous structures were labeled by TRITC-phalloidin, a specific agent for staining actin microfilaments, and identified as having 9 nm diameters by negative staining. These results indicated that PEAc1 preserved the essential characteristics of actin even with His-tag and GFP fusion, suggesting a promising potential to use GFP fusion protein in obtainning soluble plant actin isoform to analyze its physical and biochemical properties in vitro. The PEAc1-GFP was also expressed in tobacco BY2 cells, which offers a new pathway for further studying its distribution and function in vivo.

关键词： actin isoform polymerization Dnase I inhibition myosin Mg-ATPase activation expression.

Performance analysis of Gan-based high-electron-mobility transistors with postpassivation plasma treatment

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Chinese Physics B 2021年第2期30卷 563-566页

作者： Xing-Ye zhou Xin Tan Yuan-Jie Lv Guo-dong Gu zhi-Rong zhang Yan-Min Guo zhi-Hong Feng Shu-Jun Cai national Key Laboratory of Application Specific Integrated Circuit Hebei Semiconductor Research InstituteShijiazhuang 050051China

AlGan/Gan high-electron-mobility transistors(HEMTs)with postpassivation plasma treatment are demonstrated and investigated for the first *** results show that postpassivation plasma treatment can reduce the gate leakage and enhance the drain *** with the conventional devices,the gate leakage of Al Ga n/Ga n HEMTs with postpassivation plasma decreases greatly while the drain current ***-voltage measurement and frequencydependent conductance method are used to study the surface and interface *** mechanism analysis indicates that the surface traps in the access region can be reduced by postpassivation plasma treatment and thus suppress the effect of virtual gate,which can explain the improvement of DC characteristics of ***,the density and time constant of interface traps under the gate are extracted and analyzed.

关键词： Gan HEMT gate leakage trapping effect

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反向响应过程的PID控制器整定方法

测控技术 2009年第3期28卷 43-44,52页

作者：董宇华南师范大学计算机学院广东广州510631

提出了一种针对反向响应过程的PID控制器整定方法。该方法通过配置一对复数极点的位置和调整比例增益,获得令人满意的输出响应。仿真结果表明,在抑制超调和负调方面,该方法具有优于Z-n公式的性能。

关键词：反向响应 PID控制极点配置

Endoscopic features suggesting gastric cancer in biopsy-proven gastric adenoma with high-grade neoplasia

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World Journal of Gastroenterology 2014年第34期20卷 12233-12240页

作者： Jung Ho Kim Yoon Jae Kim Jungsuk An Jong Joon Lee Jae Hee Cho Kyoung Oh Kim Jun-Won Chung Kwang An Kwon dong Kyun Park Ju Hyun Kim Department of Internal Medicine Gachon University Gil Medical CenterNamdong-guIncheon 405-760South Korea Department of Pathology Gachon University Gil Medical Center21 Namdong-daero 774 beon-gilNamdong-guIncheon 405-760South Korea

AIM: To elucidate the endoscopic features that predict the cancer following endoscopic submucosal dissection (ESD) in patients with high-grade neoplasia (HGn).

关键词： Stomach neoplasms Carcinoma Adenoma Endoscopy Dissection Risk factors