检索结果-南通市图书馆

A telomere-to-telomere genome assembly of zhonghuang 13,a widely-grown soybean variety from the original center of Glycine max

在线全文

学校读者我要写书评

暂无评论

The Crop Journal 2024年第1期12卷 142-153页

作者： Anqi zhang Tangchao Kong Baiquan Sun Shizheng Qiu Jiahe guo Shuyong Ruan Yu guo Jirui guo zhishuai zhang Yue Liu zheng Hu Tao Jiang Yadong Liu Shuqi Cao Shi Sun Tingting Wu Huilong Hong Bingjun Jiang Maoxiang Yang Xiangyu Yao Yang Hu Bo Liu Tianfu Han Yadong Wang Center for Bioinformatics School of Computer Science and TechnologyHarbin Institute of TechnologyHarbin 150001HeilongjiangChina Ministry of Agriculture Key Laboratory of Soybean Biology(Beijing) Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

Soybean(Glycine max)stands as a globally significant agricultural crop,and the comprehensive assembly of its genome is of paramount importance for unraveling its biological characteristics and evolutionary ***,previous soybean genome assemblies have harbored gaps and incompleteness,which have constrained in-depth investigations into ***,we present Telomere-to-Telomere(T2T)assembly of the Chinese soybean cultivar zhonghuang 13(zh13)genome,termed zh13-T2T,utilizing PacBio Hifi and ONT ultralong *** employed a multi-assembler approach,integrating Hifiasm,NextDenovo,and Canu,to minimize biases and enhance assembly *** assembly spans 1,015,024,879 bp,effectively resolving all 393 gaps that previously plagued the reference *** annotation efforts identified 50,564 high-confidence protein-coding genes,707 of which are ***13-T2T revealed longer chromosomes,421 not-aligned regions(NARs),112 structure variations(SVs),and a substantial expansion of repetitive element compared to earlier ***,we identified 25.67 Mb of tandem repeats,an enrichment of 5S and 48S rDNAs,and characterized their genotypic *** summary,we deliver the first complete Chinese soybean cultivar T2T *** comprehensive annotation,along with precise centromere and telomere characterization,as well as insights into structural variations,further enhance our understanding of soybean genetics and evolution.

关键词： Soybean Telomere-to-Telomere assembly zhonghuang 13 Structure variations

Recent advances in the magnetic reconnection, dipolarization, and auroral processes at giant planets from the perspective of comparative planetology

在线全文

学校读者我要写书评

暂无评论

Earth and Planetary Physics 2024年第5期8卷 659-672页

作者： zhongHua Yao RuiLong guo Yong Wei Bertrand Bonfond Denis Grodent Binzheng zhang William R.Dunn ZuYin Pu Key Laboratory of Earth and Planetary Physics Institute of Geology and GeophysicsChinese Academy of Sciences College of Earth and Planetary Sciences University of Chinese Academy of Sciences Laboratoire de Physique Atmosphérique et Planétaire STAR InstituteUniversitéde Liège Shandong Provincial Key Laboratory of Optical Astronomy and Solar-Terrestrial Environment Institute of Space SciencesShandong University Department of Earth Sciences the University of Hong Kong Department of Physics and Astronomy University College London School of Earth and Space Sciences Peking University

Magnetic reconnection and dipolarization are crucial processes driving magnetospheric dynamics, including particle energization, mass circulation, and auroral processes, among others. Recent studies have revealed that these processes at Saturn and Jupiter are fundamentally different from the ones at Earth. The reconnection and dipolarization processes are far more important than previously expected in the dayside magnetodisc of Saturn and potentially Jupiter. Dayside magnetodisc reconnection was directly identified by using Cassini measurements(guo RL et al., 2018b) and was found to be drizzle-like and rotating in the magnetosphere of Saturn(Delamere et al., 2015b; Yao zh et al., 2017a; guo RL et al., 2019). Moreover, magnetic dipolarization could also exist at Saturn's dayside(Yao zh et al., 2018), which is fundamentally different from the terrestrial situation. These new results significantly improve our understanding of giant planetary magnetospheric dynamics and provide key insights revealing the physics of planetary aurorae. Here, we briefly review these recent advances and their potential implications for future investigations.

关键词： magnetosphere magnetic reconnection magnetic depolarization

Semaphorin 7A impairs barrier function in cultured human corneal epithelial cells in a manner dependent on nuclear factor-kappa B

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2024年第3期17卷 444-453页

作者： Cheng-Cheng Yang Xiu-Xia Yang Xiao-Jing zhao Heng Wang Zi-Han guo Kai Jin Yang Liu Bin-Hui Li Department of Ophthalmology the Fifth Affiliated HospitalSun Yat-sen UniversityZhuhai 519000Guangdong ProvinceChina Department of Ophthalmology Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical UniversityHangzhou 310006Zhejiang ProvinceChina Eye Institute of Xiamen University Fujian Provincial Key Laboratory of Ophthalmology and Visual ScienceSchool of MedicineXiamen UniversityXiamen 361005Fujian ProvinceChina

●AIM:To evaluate the role of semaphorin 7A(Sema7A)and its associated regulatory mechanisms in modulating the barrier function of cultured human corneal epithelial cells(HCEs).●METHODS:Barrier models of HCEs were treated with recombinant human Sema7A at concentrations of 0,125,250,or 500 ng/mL for 24,48,or 72h in *** electrical resistance(TEER)as well as Dextran-fluorescein isothiocyanate(FITC)permeability assays were conducted to assess barrier *** quantify tight junctions(TJs)such as occludin and zonula occludens-1(ZO-1)at the mRNA level,reverse transcriptionpolymerase chain reaction(RT-PCR)analysis was *** was used to examine the activity of the nuclear factor-kappa B(NF-κB)signaling pathway and the production of TJs *** analyses were employed to localize the ***-linked immunosorbent assay(ELISA)and RT-PCR were utilized to observe changes in interleukin(IL)-1β*** investigate the role of NF-κB signaling activation and IL^(-1)βin Sema7A’s anti-barrier mechanism,we employed 0.1μmol/L IκB kinase 2(IKK2)inhibitor IV or 500 ng/mL IL^(-1)receptor(IL-1R)antagonist.●RESULTS:Treatment with Sema7A resulted in decreased TEER and increased permeability of Dextran-FITC in HCEs through down-regulating mRNA and protein levels of TJs in a time-and dose-dependent manner,as well as altering the localization of ***,Sema7A stimulated the activation of inhibitor of kappa B alpha(IκBα)and expression of IL-1β.The anti-barrier function of Sema7A was significantly suppressed by treatment with IKK2 inhibitor IV or IL-1R antagonists.●CONCLUSION:Sema7A disrupts barrier function through its influence on NF-κB-mediated expression of TJ proteins,as well as the expression of IL-1β.These findings suggest that Sema7A could be a potential therapeutic target for the diseases in corneal epithelium.

关键词： human corneal epithelial barrier function transepithelial electrical resistance zonula occludens-1 occludin nuclear factor-kappa B

Sensitive analysis of amino acids in injection liquor by reversed-phase HPLC

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Chemistry 1995年第2期13卷 163-168页

作者： SHANG, zh YU, YN guo, W JIANG, H zhOU, LM CHINESE ACAD SCI DALIAN INST CHEM PHYSDALIAN 116012PEOPLES R CHINA

A convenient derivatization method of amino acids with 1-fluoro-2,4-dinitrobezene as reaction reagent and a separation system were described. The derivative amino acids were separated on a specific chemically bonded phase column with a simple linear gradient elution consisting of aqueous buffer and methanol. The eluate was detected by common ultraviolet absorption detector at 360 nm. The detection limits of amino acids were as low as 10 picomole. This method has been successfully applied to assay amino acid injection liquor used in hospital. It has good reproducibility and precision. The procedures avoid the requirements of particular derivative equipment and analyzer employed in conventional amino acid analysis.

关键词： AMINO ACIDS PRECOLUMN DERIVATIZATION REVERSED PHASE SEPARATION UV ABSORPTION DETERMINATION

掌控不结盟：美国对印尼的经济政策(1950—1965)

在线全文

学校读者我要写书评

暂无评论

山西大学学报(哲学社会科学版) 2024年第5期047卷 19-25页

作者：郭又新华东师范大学历史学系/社会主义历史与文献研究院

第二次世界大战后，印尼因其地缘战略位置、丰富的原材料和区域影响力而深受美国关注，成为世界上少数几个美国和苏联通过经济、技术和军事援助直接争夺影响力的国家之一。遏制共产主义是美国在印尼的首要目标，通过渲染“中苏经济攻势... 详细信息

第二次世界大战后，印尼因其地缘战略位置、丰富的原材料和区域影响力而深受美国关注，成为世界上少数几个美国和苏联通过经济、技术和军事援助直接争夺影响力的国家之一。遏制共产主义是美国在印尼的首要目标，通过渲染“中苏经济攻势”和对印尼共产党的恐惧，美国将印尼拖入对华经济遏制阵营，以象征性的经济技术援助、项目贷款、“粮食换和平”援助诱导印尼政府反共，继而致力于通过综合援助计划以及复苏印尼经济的多边行动，推动印尼走上面向西方的经济发展之路，融入美国的区域经济发展长期目标。

关键词：印尼美国 “中苏经济攻势” 经济技术援助

Preparation and Application of Polymyxin B Affinity Membrane Cartridge

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1997年第8期8卷 745-746页

作者： guo, W Shang, zh Yu, YN zhou, LM zhou, K CHINESE ACAD SCI DALIAN INST CHEM PHYSDALIAN 116012PEOPLES R CHINA SHANGHAI MED UNIV ZHONGSHAN HOSPSHANGHAI 200032PEOPLES R CHINA

Affinity membrane was used for the endotoxin adsorption. The matrix macropore membrane was prepared from filterpaper by chemically crosslinking, and polymyxin B was immobilized as affinity ligand. The prepared membrane cartridge can be used for the removal of endotoxin from aqueous solutions. With a cartridge of 40 sheets affinity membrane containing, the endotoxin level of the processed solution can be reduced to a minimum of 4 pg/mL.

关键词： REMOVAL

Knockout of miR396 genes increases seed size and yield in soybean

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2024年第6期66卷 1148-1157页

作者： Hongtao Xie Fei Su Qingfeng Niu Leping Geng Xuesong Cao Minglei Song Jinsong Dong Zai zheng Rui guo Yang zhang Yuanwei Deng zhanbo Ji Kang Pang Jian‐Kang zhu Jianhua zhu School of Life Sciences Anhui Agricultural UniversityHefei 230036China Research Center for Biological Breeding Technology Research Institute of Frontier ScienceAnhui Agricultural UniversityHefei 230036China Bellagen Biotechnology Co.Ltd Jinan 250000China Institute of Crop Sciences/National Nanfan Research Institute Chinese Academy of Agricultural SciencesKey Laboratory of Gene Editing Technologies(Hainan)Ministry of Agriculture and Rural AffairsSanya 572025China Southern University of Science and TechnologyShenzhen 518055China Hainan Yazhou Bay Seed Laboratory Sanya 572024China

Yield improvement has long been an important task for soybean breeding in the world in order to meet the increasing demand for food and animal *** R396 genes have been shown to negatively regulate grain size in rice,but whether mi R396 family members may function in a similar manner in soybean is ***,we generated eight soybean mutants harboring different combinations of homozygous mutations in the six soybean mi R396genes through genome editing with clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated nuclease(Cas)12SF01 in the elite soybean cultivar zhonghuang 302(zh302).Four triple mutants(mir396aci,mir396acd,mir396adf,and mir396cdf),two quadruple mutants(mir396-abcd and mir396acfi),and two quintuple mutants(mir396abcdf and mir396bcdfi)were *** found that plants of all the mir396 mutants produced larger seeds compared to zh302 *** tests showed that mir396adf and mir396cdf plants have significantly increased yield in growth zones with relatively high latitude which are suited for zh302 and moderately increased yield in lower *** contrast,mir396abcdf and mir396bcdfiplants have increased plant height and decreased yield in growth zones with relatively high latitude due to lodging issues,but they are suited for low latitude growth zones with increased yield without lodging *** together,our study demonstrated that loss-of-function of mi R396 genes leads to significantly enlarged seed size and increased yield in soybean,providing valuable germplasms for breeding high-yield soybean.

关键词： Cas12SF01 CRISPR/Cas miR396 seed size soybean yield

在线全文

学校读者我要写书评

暂无评论

基于注意力融合特征增强的座舱表情识别模型

汽车工程 2024年第9期46卷 1697-1706,1686页

作者：罗玉涛郭丰瑞华南理工大学机械与汽车工程学院广州510640 广东省汽车工程重点实验室广州510640

针对智能座舱驾驶员表情识别深度学习模型准确率和实时性难以兼顾的问题,提出一种基于注意力融合与特征增强网络的表情识别模型EmotionNet。模型以GhostNet为基础,在特征提取模块内利用两个检测分支融合坐标注意力和通道注意力机制,实... 详细信息

针对智能座舱驾驶员表情识别深度学习模型准确率和实时性难以兼顾的问题,提出一种基于注意力融合与特征增强网络的表情识别模型EmotionNet。模型以GhostNet为基础,在特征提取模块内利用两个检测分支融合坐标注意力和通道注意力机制,实现注意力机制互补与对重要特征的全方位关注;建立特征增强颈部网络以融合不同尺度特征信息;最终通过头部网络实现不同尺度特征信息决策级融合。在训练中则引入迁移学习思想和中心损失函数以进一步提高模型的识别准确性。在RAF-DB和KMU-FED数据集实验中,模型分别取得85.23%和99.95%识别准确率,并达到59.89 FPS的识别速度。EmotionNet平衡了识别准确率和实时性,达到了较为先进的水平并具备一定的智能座舱表情识别任务的适用性。

关键词：智能座舱表情识别注意力机制特征增强网络

ENDOTOXIN REMOVAL BY MEMBRANE AFFINITY CHROMATOGRAPHY

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1995年第10期6卷 919-922页

作者： guo, W Shang, zh Yu, YN zhou, LM Zun, ZY Ma, LR Chen, MY Chinese Acad Sci Dalian Inst Chem Phys Dalian 116012 Peoples R China Acad Mil Med Sci Inst Radiat Med Beijing 100850 Peoples R China

Endotoxin was removed by membrane affinity chromatography. Macropore cellulose membrane was prepared from filter paper by alkaline treatment and chemical crosslinking, and was used as stationary phase for affinity chromatography. The matrix membrane was derived by hexamethylenediamine and activated by glutaraldehyde before histidine was immobilized as affinity ligand. A membrane cartridge containing 40 sheets of affinity membrane was also prepared, which can be used to remove pyrogen from aqueous solutions.

关键词：