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Chromosome-level assembly of triploid genome of Sichuan pepper(Zanthoxylum armatum)

Horticultural Plant Journal 2024年第2期10卷 437-449页

作者： lizhi Song Yue Huang Hao Zuo Ning Tang Zhengguo li Wenbiao Jiao Feng Xu Qiang Xu zexiong Chen National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops Huazhong Agricultural UniversityWuhanHubei 430070China Chongqing Key Laboratory of Economic Plant Biotechnology Chongqing University of Arts and SciencesChongqing 402160China School of life Sciences Chongqing UniversityChongqing 400030China College of Horticulture and Gardening Yangtze UniversityJingzhouHubei 434025China College of Informatics Huazhong Agricultural UniversityWuhanHubei 430070China

As an important spice species in Rutaceae, the Sichuan pepper (Zanthoxylum armatum) can provide pungent and numbing taste, as well as aroma in its mature fruit. Here we assembled a chromosome-level genome of green prickly ash which was widely cultivated in a major production area including Chongqing and Sichuan province, China. We generated 712 Gb (~112×) PacBio long reads and 511 Gb (~82×) Hi-C data, and yielded an assembly of 99 pseudochromosomes with total size of 5.32 Gb and contig N50 of 796 kb. The genomic analyses and cytogenetic experiments both indicated that the cultivarZhuye Huajiao’ was a triploid. We identified a Zanthoxylum-specific whole genome duplication event emerging about 24.8 million years ago (Mya). We also detected a transposition burst event (0.3-0.4 Mya) responsible for the large genome size of Z. armatum. Metabolomic analysis of the Zanthoxylum fruits during development stages revealed profiles of39 volatile aroma compounds and 528 secondary metabolites, from which six types of sanshools were identified. Based on metabolomic and transcriptomic network analysis, we screened candidate genes encoding long chain acyl-CoA synthetase, fatty acid desaturase,branched-chain amino acid aminotransferase involved in sanshool biosynthesis and three genes encoding terpene synthase during fruit development. The multi-omics data provide insights into the evolution of Zanthoxylum and molecular basis of numbing and aroma flavor of Sichuan pepper.

关键词： Zanthoxylum armatum Genome Sanshool Terpenoid

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Sleep Deprivation Selectively Down-Regulates Astrocytic 5-HT2B Receptors and Triggers Depressive-like Behaviors via Stimulating P2X7 Receptors in Mice

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Neuroscience Bulletin 2020年第11期36卷 1259-1270页

作者： Maosheng Xia zexiong li Shuai li Shanshan liang Xiaowei li Beina Chen Manman Zhang Chengyi Dong Alexei Verkhratsky Dawei Guan Baoman li Practical Teaching Centre School of Forensic MedicineChina Medical UniversityShenyang 110122China Department of Orthopaedics The First HospitalChina Medical UniversityShenyang 110001China Faculty of Biology Medicine and HealthThe University of ManchesterManchester M139PLUK Department of Forensic Pathology School of Forensic MedicineChina Medical UniversityShenyang 110122China Department of Poison Analysis School of Forensic MedicineChina Medical UniversityShenyang 110122China

Chronic loss of sleep damages health and disturbs the quality of life.Long-lasting sleep deprivation(SD)as well as sleep abnormalities are substantial risk factors for major depressive disorder,although the underlying mechanisms are not clear.Here,we showed that chronic SD in mice promotes a gradual elevation of extracellular ATP,which activates astroglial P2X7 receptors(P2X7Rs).Activated P2X7Rs,in turn,selectively down-regulated the expression of 5-HT2B receptors(5-HT2BRs)in astrocytes.Stimulation of P2X7Rs induced by SD selectively suppressed the phosphorylation of AKT and FoxO3 a in astrocytes,but not in neurons.The overexpression of FoxO3a in astrocytes inhibited the expression of 5-HT2BRs.Down-regulation of 5-HT2BsRs instigated by SD suppressed the activation of STAT3 and relieved the inhibition of Ca2+-dependent phospholipase A2.This latter cascade promoted the release of arachidonic acid and prostaglandin E2.The depression-like behaviors induced by SD were alleviated in P2X7R-KO mice.Our study reveals the mechanism underlying chronic SD-induced depression-like behaviors and suggests 5-HT2BRs as a key target for exploring therapeutic strategies aimed at the depression evoked by sleep disorders.

关键词： Astrocyte Sleep deprivation P2X7 receptor 5-HT2B receptor FoxO3a

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Iron Aggravates the Depressive Phenotype of Stressed Mice by Compromising the Glymphatic System

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Neuroscience Bulletin 2020年第12期36卷 1542-1546页

作者： Shanshan liang Yan Lu zexiong li Shuai li Beina Chen Manman Zhang Binjie Chen Ming Ji Wenliang Gong Maosheng Xia Alexei Verkhratsky Xu Wu Baoman li Practical Teaching Center School of Forensic MedicineChina Medical UniversityShenyang 110122China Key Laboratory of the Ministry of Health in Congenital Malformation The Affiliated Shengjing Hospital of China Medical UniversityShenyang 110004China Department of Orthopedics The First HospitalChina Medical UniversityShenyang 110001China Faculty of Biology Medicine and HealthThe University of ManchesterManchesterUK Achucarro Center for Neuroscience IKERBASQUE48011 BilbaoSpain Department of Forensic Pathology School of Forensic MedicineChina Medical UniversityShenyang 110122China

Dear Editor,The pathophysiologic al mechanisms underlying mood disorders including major depressive disorder(MDD)remain to be fully characterized.Iron is a key component in the development of the central nervous syste... 详细信息

关键词： impairment Iron lymphatic

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配戴角膜塑形镜对青少年近视患者眼表的影响

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汕头大学医学院学报 2024年第2期37卷 71-75页

作者：李浩李丹娄智因陈泽雄林世斌汕头大学·香港中文大学联合汕头国际眼科中心广东汕头515041

目的:探讨配戴角膜塑形镜对青少年近视患者眼表的影响以及不同设计类型的角膜塑形镜对眼表影响的差别。方法:前瞻性队列研究。选取2021年6月—2022年3月在汕头国际眼科中心就诊选择配戴角膜塑形镜的41例青少年近视患者,选其右眼为受试... 详细信息

目的:探讨配戴角膜塑形镜对青少年近视患者眼表的影响以及不同设计类型的角膜塑形镜对眼表影响的差别。方法:前瞻性队列研究。选取2021年6月—2022年3月在汕头国际眼科中心就诊选择配戴角膜塑形镜的41例青少年近视患者,选其右眼为受试眼。根据患者试戴角膜塑形镜类型分为VST组(21只眼)和CRT组(20只眼)。VST组男性8例,女性13例,年龄(10.7±1.7)岁,CRT组男性8例,女性12例,年龄(10.4±1.8)岁。分别于戴镜前和戴镜后1、3个月对研究对象的眼表疾病指数(Ocular Surface Disease Index,OSDI)量表评分、泪河高度、泪膜破裂时间、泪液基础分泌量、眼红指数、角膜上皮荧光素染色进行随访检查。结果:青少年近视患者在配戴角膜塑形镜后1个月、3个月与戴镜前相比,OSDI评分、眼红指数升高,泪膜破裂时间降低(均P0.05)。戴镜后角膜上皮点染增加(主要以Ⅰ、Ⅱ级点染为主),且戴镜后1个月与3个月相比无差异(Z=-0.124,P>0.05)。两组患者戴镜前后的OSDI评分、泪河高度、泪膜破裂时间、泪液基础分泌量、眼红指数以及角膜上皮荧光素染色差异无统计学意义(均P>0.05)。结论:短期配戴角膜塑形镜不会对青少年近视患者的泪液分泌量造成影响,但会引起轻度的不适感,同时也会降低泪膜的稳定性以及增加角膜上皮损伤。VST型和CRT型角膜塑形镜对眼表的影响无差异。

关键词：角膜塑形镜青少年近视眼表

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Rare-earth metal catalysts for alkene hydrosilylation

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Science China Chemistry 2019年第5期62卷 571-582页

作者： Deshuai liu Boyu liu zexiong Pan Jianfeng li Chunming Cui State Key Laboratory of Elemento-organic Chemistry and College of Chemistry Nankai University Collaborative Innovation Center of Chemical Science and Engineering (Tianjin)

Rare-earth metal catalyzed hydrosilylation of alkenes has emerged as a powerful and selective strategy for the synthesis of organosilanes. This transformation can offer distinctive catalytic sequences and reaction patterns from other catalysts because of the high electropositivity and lack of oxidative-addition process of rare-earth metal. This review summarizes the rare-earth metal catalysts for hydrosilylation of alkene according to the type of ligands. The synthesis and structure of rare-earth metal catalysts,the substrate scope as well as some preliminary structure-activity relationship and mechanism are discussed.

关键词： rare-earth metal hydrosilylation alkene catalysis mechanism

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Improving prodeoxyviolacein production via multiplex SCRaMbLE iterative cycles

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Frontiers of Chemical Science and Engineering 2018年第4期12卷 806-814页

作者： Juan Wang Bin Jia zexiong Xie Yunxiang li Yingjin Yuan Key Laboratory of Systems Bioengineering (Ministry of Education) School of Chemical Engineering and Technology Tianjin UniversityTianjin 300072China SynBio Research Platform Collaborative Innovation Center of Chemical Science and Engineering (Tianjin)Tianjin University Tianjin 300072China

The synthetic chromosome rearrangement and modification by loxP-mediated evolution (SCRaMbLE) system has been used to improve prodeoxyviolacein (PDV) production in haploid yeast containing chromosome synV. To rapidly and continuously generate genome diversification with the desired phenotype, the multiplex SCRaMbLE iterative cycle strategy has been developed for the screening of high PDV production strains. Whole-genome sequencing analysis reveals large duplications, deletions, and even the whole genome duplications. The deletion of YER151C is proved to be responsible for the increase. This study demonstrates that artificial DNA rearrangement can be used to accelerate microbial evolution and the production of biobased chemicals.

关键词： synthetic biology genome rearrangement prodeoxyviolacein SCRaMbLE Saccharomyces cerevisiae

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Jianpijiedu Fang improves survival of hepatocarcinoma mice by affecting phosphatase and tensin homolog, phosphoinositide 3-kinase, and focal adhesion kinase

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Journal of Traditional Chinese Medicine 2013年第4期33卷 479-485页

作者： Baoguo Sun Jun Meng Ting Xiang zexiong Chen Yulong li lisha Lu Shijun Zhang Xiaolin Chen Department of Traditional Chinese Medicine the First Affiliated Hospital of Sun Yat-sen University

OBJECTIVE: To investigate the effect of Jianpijiedu Fang (JPJDF) on phosphatase and tensin homolog (PTEN), phosphoinositide 3-kinase (PI3K), and focal adhesion kinase (FAK), and on the survival of hepatocellular carcinoma (HCC) nude mice. METHODS: Forty male nude mice were randomly divided into 4 groups. Human HCC tissue was implanted in the livers of three groups. After 24 h, the three groups were treated respectively with JPJDF (37.5 g/kg), saline (20 mL/kg) and Tegafur (FT-207, 160 mg/kg) once a day for 10 weeks. The control group without implanting the tissue was concurrently treated with saline (20 mL/kg). The survival data and body weight of all mice were recorded, and expression levels of PTEN, PI3K and FAK in normal tissue and cancer tissue of the livers were eval-uated with immunohistochemical method. RESULTS: The cumulative survival rate of the mice in the JPJDF group was higher than those of the other groups. The rate of weight loss was the lowest in JPJDF group. The survivability and weight loss rate in FT-207 group were the poorest in all groups. The expression intensity of PTEN was higher in normal tissues than in cancer tissues, and lower in the normal tissues of HCC models than in that of mice without HCC. The PTEN expression intensity in normal tissue and cancer tissue from mice treated with FT-207 were lower than that from the mice treated with JPJDF or saline.The expression intensity of PI3K was higher in cancer tissue than in normal tissue. The PI3K expression intensity was the lowest in normal tissue and cancer tissue from mice treated with JPJDF, and the intensity from mice treated with FT-207 was the highest. In mice treated with JPJDF, the expression intensity of FAK was higher in the normal tissue and lower in the cancer tissue than those of the other treatment groups. CONCLUSION: The mechanism accounting for the prolonged survival of HCC-bearing mice treated with JPJDF might be related to the reduction in weight loss and the benign regulation of PTEN, PI3K, and FAK.

关键词：粘着斑激酶累积存活率裸小鼠磷酸肌醇同源物磷酸酶肝癌蛋白

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超低掺量氧化石墨烯的分散行为及其对水泥基材料结构与性能的影响

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复合材料学报 2023年第4期40卷 2296-2307页

作者：吴磊吕生华李泽雄李尧刘雷鹏陕西科技大学轻工科学与工程学院西安710021

研究了氧化石墨烯(GO)纳米片层在水相及分散剂作用下的存在状态及分散行为,发现GO在水泥基材料中存在掺量大、成本高及应用效果不稳定不显著的主要原因是:GO纳米片层容易团聚导致其在水泥基体中分散不均匀。为此制备了两性聚羧酸分散剂(... 详细信息

研究了氧化石墨烯(GO)纳米片层在水相及分散剂作用下的存在状态及分散行为,发现GO在水泥基材料中存在掺量大、成本高及应用效果不稳定不显著的主要原因是:GO纳米片层容易团聚导致其在水泥基体中分散不均匀。为此制备了两性聚羧酸分散剂(APC)及与GO的复合物(APC-GO),研究发现GO在APCGO复合物中不再以团簇式聚集态存在,而是主要吸附在APC多支链分子上并呈现多支链状的分散状态,通过掺入APC-GO复合物引入与水泥质量比为0.0003%的超低掺量GO,能够显著提高水泥基材料的力学性能和耐久性。SEM显示掺入APC-GO的水泥基材料具有规整致密的微观结构形貌,说明了GO在水泥基体中能够均匀分散,且对水泥水化产物的形貌和结构具有规整性调控效果,研究结果对GO在水泥基材料中的应用具有指导意义。

关键词：氧化石墨烯两性聚羧酸减水剂复合物分散行为超低掺量

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基于膜解剖之四纵两横六层面法的腹腔镜根治性膀胱切除术的临床应用价值(“大家泌尿网”观看手术视频)

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现代泌尿外科杂志 2024年第5期29卷 399-405页

作者：保东平钟培锋伍国豪李浩民陈东江胡先国吴炳权陈征郭泽雄叶东明赖彩永暨南大学附属第一医院泌尿外科广东广州510630 暨南大学附属第六医院泌尿外科广东东莞523570 肇庆市第一人民医院泌尿外科广东肇庆526000 阳西县人民医院泌尿外科广东阳江529800 暨南大学附属第六医院泌尿外科研究所广东东莞523570

目的探讨基于膜解剖的四纵两横六层面法的腹腔镜根治性膀胱切除术在膀胱癌治疗中的临床价值。方法回顾性分析暨南大学第一附属医院和第六附属医院泌尿外科2015年1月—2022年6月因膀胱癌行腹腔镜根治性膀胱切除术的51例患者的临床资料,... 详细信息

目的探讨基于膜解剖的四纵两横六层面法的腹腔镜根治性膀胱切除术在膀胱癌治疗中的临床价值。方法回顾性分析暨南大学第一附属医院和第六附属医院泌尿外科2015年1月—2022年6月因膀胱癌行腹腔镜根治性膀胱切除术的51例患者的临床资料,按照“四纵两横六层面法”的手术思路,采用膜解剖技术完成膀胱根治性切除。其中术前性功能正常的男性患者行预先盆神经丛显露的保留性神经的膀胱癌根治术。结果本组患者手术均顺利完成,无中转开放手术。手术时间(502.52±108.99)min,术中失血量(275.96±155.18)mL,术后进食时间(4.14±2.41)d,术后住院时间(16.37±4.85)d,淋巴结清扫数量(17.98±11.48)枚,平均随访(30.27±19.39)个月。在末次随访中,患者均未出现Clavien≥3级的并发症,总体生存率(OS)、肿瘤特异性生存率(TSS)和无复发生存率(RFS)的比率分别为82.4%、92.2%和88.2%。淋巴结阳性和阴性患者的OS分别为60.0%和84.8%、RFS为60.0%和91.3%。行预先显露盆神经丛的保留性神经的膀胱癌根治术患者中18例采用原位新膀胱术,其日间及夜间尿控率分别为83.3%和72.2%,17例在术后6个月内恢复了性功能。结论基于筋膜解剖的四纵两横六层面法腹腔镜膀胱根治性切除术切实可行,手术步骤程序化、肿瘤控制效果好、出血少、术后并发症少、术后恢复快。

关键词：膜解剖膀胱癌根治性膀胱切除术四纵两横六层面法

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The novel male meiosis recombination regulator coordinates the progression of meiosis prophase Ⅰ

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Journal of Genetics and Genomics 2020年第8期47卷 449-464页

作者： Miao li Haiwei Feng zexiong lin Jiahuan Zheng Dongteng liu Rui Guo Junshi li Raymond H.W.li Ernest H.Y.Ng Michael S.Y.Huen P.Jeremy Wang William S.B.Yeung Kui liu Shenzhen Key Laboratory of Fertility Regulation Center of Assisted Reproduction and EmbryologyThe University of Hong Kong-Shenzhen HospitalShenzhen518000China Department of Obstetrics and Gynecology Li Ka Shing Faculty of MedicineThe University of Hong KongHong Kong999077China Department of Biomedical Sciences University of Pennsylvania School of Veterinary MedicinePhiladelphiaPA19104USA School of Biomedical Sciences Li Ka Shing Faculty of MedicineThe University of Hong KongHong Kong999077China

Meiosis is a specialized cell division for producing haploid gametes in sexually reproducing organisms.In this study,we have independently identified a novel meiosis protein male meiosis recombination regulator(MAMERR)/4930432 K21 Rik and showed that it is indispensable for meiosis prophase I progression in male mice.Using super-resolution structured illumination microscopy,we found that MAMERR functions at the same double-strand breaks as the replication protein A and meiosis-specific with OB domains/spermatogenesis associated 22 complex.We generated a Mamerr-deficient mouse model by deleting exons 3 e6 and found that most of Mamerrà/àspermatocytes were arrested at pachynema and failed to progress to diplonema,although they exhibited almost intact synapsis and progression to the pachytene stage along with XY body formation.Further mechanistic studies revealed that the recruitment of DMC1/RAD51 and heat shock factor 2 ebinding protein in Mamerrà/àspermatocytes was only mildly impaired with a partial reduction in double-strand break repair,whereas a substantial reduction in ubiquitination on the autosomal axes and on the XY body appeared as a major phenotype in Mamerrà/àspermatocytes.We propose that MAMERR may participate in meiotic prophase I progression by regulating the ubiquitination of key meiotic proteins on autosomes and XY chromosomes,and in the absence of MAMERR,the repressed ubiquitination of key meiotic proteins leads to pachytene arrest and cell death.

关键词： Meiosis 4930432K21Rik Recombination Ubiquitination

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