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Characterizing cellular mechanical phenotypes with mechanonode-pore sensing

Microsystems & Nanoengineering 2018年第1期4卷 438-449页

作者： junghyun Kim Sewoon Han Andy Lei Masaru Miyano jessica Bloom Vasudha Srivastava Martha R.Stampfer zev j.gartner Mark A.LaBarge Lydia L.Sohn Department of Mechanical Engineering University of California at BerkeleyBerkeleyCA 94720-1740USA Department of Bioengineering University of California at BerkeleyBerkeleyCA 94720-1762USA Department of Population Sciences Beckman Research InstituteCity of HopeDuarteCA 91010USA Department of Pharmaceutical Chemistry University of CaliforniaSan FranciscoSan FranciscoCA 94143USA Biological Systems and Engineering Division Lawrence Berkeley National LaboratoryCA 94720USA Graduate Program in Bioengineering University of CaliforniaBerkeleyand University of CaliforniaSan FranciscoBerkeleyCA 94720USA

The mechanical properties of cells change with their differentiation,chronological age,and malignant ***,these properties may be useful label-free biomarkers of various functional or clinically relevant cell ***,we demonstrate mechano-node-pore sensing(mechano-NPS),a multi-parametric single-cell-analysis method that utilizes a four-terminal measurement of the current across a microfluidic channel to quantify simultaneously cell diameter,resistance to compressive deformation,transverse deformation under constant strain,and recovery time after *** define a new parameter,the whole-cell deformability index(wCDI),which provides a quantitative mechanical metric of the resistance to compressive deformation that can be used to discriminate among different cell *** wCDI and the transverse deformation under constant strain show malignant MCF-7 and A549 cell lines are mechanically distinct from non-malignant,MCF-10A and BEAS-2B cell lines,and distinguishes between cells treated or untreated with cytoskeleton-perturbing small *** categorize cell recovery time,ΔT_(r),as instantaneous(ΔTr~0 ms),transient(ΔT_(r)≤40 ms),or prolonged(ΔT_(r)>40 ms),and show that the composition of recovery types,which is a consequence of changes in cytoskeletal organization,correlates with cellular *** the wCDI and cell-recovery time,mechano-NPS discriminates between sub-lineages of normal primary human mammary epithelial cells with accuracy comparable to flow cytometry,but without antibody ***-NPS identifies mechanical phenotypes that distinguishes lineage,chronological age,and stage of malignant progression in human epithelial cells.

关键词： microfluidics mechanical phenotyping node-pore sensing biosensors label-free

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闽北方言的调值与"弱化声母"的拟测

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方言 2004年第1期26卷 54-75页

作者： zev Handel 陈宝贤 Departmet of Asian Languages and Literature University of WashingtonSeattle 北京大学中文系 2002级博士生北京100871

罗杰瑞所拟测的原始闽语"弱化声母"而分化出的闽北方言的声调,从相关调型的音高来看,遵循了完全一致的类型。本文证明,该音高类型不可能解释为借入或层次造成的。它构成了支持罗杰瑞拟音的强有力证据,同时也为确定"弱化"声母的语音实质... 详细信息

罗杰瑞所拟测的原始闽语"弱化声母"而分化出的闽北方言的声调,从相关调型的音高来看,遵循了完全一致的类型。本文证明,该音高类型不可能解释为借入或层次造成的。它构成了支持罗杰瑞拟音的强有力证据,同时也为确定"弱化"声母的语音实质提供了依据。本研究涉及八个闽北方言的材料,其中之一是本文首次描写的。文章证明,其中的某些方言构成了源头上有联系的土语群,该土语群的原始声调系统(包括绝对音高值)可以有把握地构拟。通过进一步的比较,整个原始闽北语的声调系统也可以构拟出来。原始闽北语具有八个调类和三套发音方法不同的塞音、塞擦音声母。根据声调的音高模式和普遍音理,本文将与罗杰瑞原始闽语"弱化声母"相对应的原始闽北语的声母,构拟为伴有气嗓音除阻的浊声母。

关键词：闽北方言调值声母音高声调

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Harvesting of Mouse Embryos at 0.5 Dpc as a Tool to Reduce Animal Use: Data from C57BL/6j, B6*129 and FVB/Nj Strains

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Open journal of Animal Sciences 2020年第2期10卷 254-265页

作者： Sofia Lamas Filipa Franquinho Marlene Morgado Fátima gartner Irina Amorim Institute for Research and Innovation in Health (i3S) University of Porto Porto Portugal Animal Facility i3S/IBMC Porto Portugal Institute for Molecular and Cell Biology (IBMC) Porto Portugal Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP) Porto Portugal Department of Pathology and Molecular Immunology Institute of Biomedical Sciences Abel Salazar (ICBAS) University of Porto Porto Portugal

Superovulation is used to stimulate the production and release of large amounts of oocytes in mice by using two hormones that mimic FSH (PMSG) and LH (hCG) effects. Since superovulation can have a negative impact on oocyte and embryo development, this investigation aimed to compare two alternatives for 2-cells embryo collection in order to reduce the number of females and to benefit from the superovulation process. Data from mouse embryo collection from our facility was analyzed to compare the number of 2-cells embryos collected at 1.5 dpc and the number of 2-cells embryos obtained after overnight incubation of 1-cell embryos, collected at 0.5 dpc. Genetically modified mouse strains with a similar background (C57BL/6j, B6*129 and FVB/Nj) were analyzed and for strains at a C57BL/6j and B6*129 background, the number of 2-cells embryos obtained after incubation was significantly higher when compared to the number of 2-cells embryos collected at 1.5 dpc (1.4-fold and 1.7-fold, respectively). C57BL/6j wild type mice had similar results with a higher number of 2-cells embryos when collection was performed at 0.5 dpc followed by incubation (1.4-fold). These results can help the planning of 2-cells embryo harvesting by reducing the number of females needed for this procedure.

关键词： 0.5 Dpc Embryos 1.5 Dpc Embryos Incubation Superovulation

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信息通讯应用质变重塑存储器市场与技术

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电子测试 2004年第1期17卷 54-62页

作者： j.Y.

根据gartner数据显示,2002年全球存储器市场销售值达284亿美元,其中只有DRAM与NAND型Flash呈现正成长的趋势,其它市场如SRAM、NOR型Flash、EPROM(Erasable Programmable Read-Only Memory)、Mask ROM(Mask Read-Only Memory)与其它存储器都呈现不同程度的衰退。进入2003年之后,除了DRAM与NAND型Flash依旧维持正成长之外,SRAM与NOR型Flash亦有所表现,因此2003年全球存储器市场估计可达343亿美元的销售值,比起2002年成长了21％。

关键词：存储器市场 DRAM NAND SRAM EPROM OUM

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