检索结果-南通市图书馆

NLRP3 inflammasome-related pyroptosis aggravates the airway inflammation in toluene diisocyanate-induced asthmatic mice

在线全文

学校读者我要写书评

暂无评论

NLRP3 inflammasome-related pyroptosis aggravates the airway ...

Attenuation of rheumatoid arthritis through the inhibition of caspase3/GSDME-mediated pyroptosis induced by TNF-α

第十三届全国免疫学学术大会

作者： Jian Zhuang Yi He Haiyan Cui Guihu Luo Fangyuan Yang zeqing zhai Yanan Zhang Erwei Sun The Third Affiliated Hospital of Southern Medical University

Background: Toluene diisocyanate(TDI), the most common inhalation of occupational asthma, is proven to drive bronchial epithelium damage and IL-1β production in patients with TDI-induced asthma and asthmatic *** a kind of proinflammatory cell death, NLRP3 inflammasome-related pyroptosis is characterized by cell injury and IL-1β *** this study, we investigated whether NLRP3 inflammasome-related pyroptosis was implicated in the development of bronchial epithelial cell injury and airway inflammation in TDI-induced ***: 16 HBE cells(a human bronchial epithelial cell line) were stimulated with TDI-HSA conjugates in *** cell viability, and the concentrations of LDH, IL-1β and IL-18 in the supernatant were *** vivo, male BALF/c mice were sensitized and challenged with TDI to establish asthma ***950, the specific inhibitor of NLRP3 inflammasome, was administered by intraperitoneal *** the airway hyperresponsiveness(AHR) and airway inflammation in TDI-induced asthmatic mice were ***: TDI-HSA conjugates reduced the bronchial epithelial cell viability in a time and dose-dependent ***, TDI-HSA increased the ratio of caspase-1PI cells, as well as the levels of LDH, IL-1β and ***, MCC950 significantly blocked TDI-HSA-induced bronchial epithelial *** TDI-induced asthmatic mice, administration with MCC950 attenuated AHR and airway inflammatory cell infiltration by inhibiting the activation of caspase-1 and pyroptotic executioner GSDMD in the ***, NLRP3 inflammasome inhibitor could decrease the levels of IL-1β in the plasma and BALF, accompanied by lower concentrations of IgE in the plasma and Th2-related cytokines in the ***: These data suggested that bronchial epithelial pyroptosis played a critical role in the pathogenesis of TDI-induced asthma via activation of NLRP3 inflammasome, and cleavage of caspase-1 and ***, inhibition of NLRP3 inflammas

关键词： Toluene diisocyanate-induced asthma NLRP3 inflammasome Bronchial epithelial pyroptosis

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Attenuation of rheumatoid arthritis through the inhibition o...

Alantolactone-loaded chitosan/hyaluronic acid nanoparticles suppress psoriasis by deactivating STAT3 pathway and restricting immune cell recruitment

第十四届全国免疫学学术大会

作者： zeqing zhai Fangyuan Yang Wenchao Xu Jiaochan Han Guihu Luo Yehao Li Jian Zhuang Hongyu Jie Xing Li Xingliang Shi Han Xinai Xiaoqing Luo Rui Song Yonghong Chen Jianheng Liang Shufan Wu Yi He Erwei Sun 南方医科大学第三附属医院(广东省骨科研究院)风湿免疫科

To determine the role of gasdermin E(GSDME)-mediated pyroptosis in the pathogenesis and progression of rheumatoid arthritis(RA), and explore the potential therapeutic targets for ***: The expression and activation of caspase3 and GSDME in the synovium, macrophages, and monocytes of RA patients was detected by immunohistochemistry, immunofluorescence, and western blot analysis. The correlation of activated GSDME with RA disease activity was evaluated. The pyroptotic ability of monocytes from RA patients was also tested. In addition, the effect of TNF-α on caspase3/GSDME-mediated pyroptosis of monocytes and macrophages was investigated. Furthermore, mice lacking Gsdme were subjected to collagen-induced arthritis, and the incidence and severity of arthritis was ***: Monocytes and synovial macrophages from RA patients showed increased expression of activated caspase3,GSDME, and GSDME-N. The expression of GSDME-N in monocytes from RA patients correlated positively with the disease activity. Monocytes from RA patients with higher GSDME levels were more susceptible to pyroptosis. Furthermore, TNF-α induced pyroptosis in monocytes and macrophages by activating the caspase3/GSDME pathway. The use of a caspase3 inhibitor and silencing of GSDME significantly blocked TNF-α–induced pyroptosis. Gsdme deficiency effectively alleviated arthritis in a collagen-induced arthritis mice ***: These Results support a pathogenic role of GSDME in RA and provide an alternative mechanism for RA pathogenesis involving TNF-α, which activates GSDME-mediated pyroptosis of monocytes and macrophages in RA. In addition, targeting GSDME might be a potential therapeutic approach for RA.

关键词： Rheumatoid Arthritis(RA) Pyroptosis Gasdermin E(GSDME) Tumor necrosis factor α(TNF-α) Collagen-Induced Arthritis(CIA)

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Asian Journal of Pharmaceutical Sciences 2022年第2期17卷 268-283页

作者： Ruijie Chen Yuan-Yuan zhai Lining Sun zeqing Wang Xing Xia Qing Yao Longfa Kou Department of Pharmacy the Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhou 325027China School of Pharmaceutical Sciences Wenzhou Medical UniversityWenzhou 325035China

Psoriasis is a common chronic immune-mediated skin disease characterized by hyperproliferation and aberrant differentiation of keratinocytes and massive infiltration of inflammatory immune *** studies showed that Signal Transducer and Activator of Transcription 3(STAT3),which plays an important role in cell survival,proliferation,differentiation,angiogenesis,and immune responses,is constitutively activated in epidermal keratinocytes of human psoriatic skin *** addition,STAT3 promotes the differentiation and expansion of T cells and facilitates cytokine production,thereby exacerbating the condition of ***(ALT)is a sesquiterpene lactone compound that could selectively suppress STAT3 activation,but its effectiveness and application in psoriasis treatment have not been *** this study,we developed ALT loaded chitosan/hyaluronic acid nanoparticles(CHALT),and investigated its therapeutic potential for psoriasis *** effectively abrogated the hyperproliferation by inducing ROS-mediated apoptosis with loss of mitochondrialmembrane potential,and also inhibited IL-6-induced STAT3 signaling activation and inflammatory reaction in HaCaT cell *** an Imiquimod(IMQ)-induced psoriasis model,the topical treatment of psoriasis lesions with CHALT effectively attenuated the STAT3 hyperactivation within keratinocytes and ameliorated the symptoms of *** addition,it was found that CHALT restricted the recruitment of immune *** results indicated that ALT-based nanoformulation CHALT holds great potential for psoriasis therapy.

关键词： Psoriasis Alantolactone STAT3 Chitosan Nanoparticle

应用双能CT与定量CT对椎体骨密度测量的对照研究

在线全文

学校读者我要写书评

暂无评论

中国骨质疏松杂志 2017年第2期23卷 159-162页

作者：王平和建伟黄刚毛泽庆周星张文文翟亚楠甘肃省人民医院放射科甘肃兰州730000 兰州大学第一医院放射科甘肃兰州730000

目的应用双能CT(DECT)及定量CT(QCT)测量腰椎骨密度,评价利用双能CT测量骨密度(BMD)的可行性。方法对56名志愿者采用DECT检查,获得钙值图,测量骨钙CT值及骨髓CT值,同时应用QCT测量骨密度(BMD),分析骨钙CT值及骨髓CT值与BMD值的相关性。... 详细信息

目的应用双能CT(DECT)及定量CT(QCT)测量腰椎骨密度,评价利用双能CT测量骨密度(BMD)的可行性。方法对56名志愿者采用DECT检查,获得钙值图,测量骨钙CT值及骨髓CT值,同时应用QCT测量骨密度(BMD),分析骨钙CT值及骨髓CT值与BMD值的相关性。结果腰椎椎体骨钙CT值与BMD值呈显著正相关(L1-5椎体Pearson相关系数分别为:r=0.715,0.692,0.739,0.673,0.686,P<0.01);骨髓CT值与BMD值呈正相关(L1-5椎体Pearson相关系数分别为r=0.343、0.315、0.439、0.440、0.456,L5椎体P<0.05,其余椎体P<0.01)。结论 DECT所测量腰椎骨钙CT值及骨髓CT值与QCT所测BMD值密切相关,可定量反映腰椎BMD变化。

关键词： X线计算机体层摄影双能量CT 骨密度

应用CT骨纹理分析与定量CT评价椎体骨密度的对照研究

在线全文

学校读者我要写书评

暂无评论

甘肃医药 2019年第4期38卷 317-319页

作者：曲红光王平马彬黄刚毛泽庆和建伟翟亚楠甘肃省人民医院甘肃兰州730000 兰州大学第一医院甘肃兰州730000

目的:以定量CT测量椎体骨密度为对照,探讨椎体CT纹理分析评价骨结构及骨密度的应用价值。方法:收集2015年12月至2017年5月在我院因急腹症、泌尿系结石及腹部良性占位病变就诊的60名患者。采用腹部CT平扫检查,应用定量CT测量L1-5椎体骨密... 详细信息

目的:以定量CT测量椎体骨密度为对照,探讨椎体CT纹理分析评价骨结构及骨密度的应用价值。方法:收集2015年12月至2017年5月在我院因急腹症、泌尿系结石及腹部良性占位病变就诊的60名患者。采用腹部CT平扫检查,应用定量CT测量L1-5椎体骨密度,应用Omni-Kinetics纹理分析软件测量L3椎体骨松质骨纹理相关参数并绘制直方图,分析椎体骨密度值与骨纹理相关参数间的相关性。结果:腰椎各椎体骨密度值以L3椎体最低,相邻椎体骨密度值略高。L3椎体骨密度值与纹理分析参数中的最小强度、最大强度、平均强度、均值、RMS、峰度、一致性、均方根值及平均正灰度呈正相关,与均差呈负相关。结论:采用CT纹理分析松质骨CT图像灰度信息,可在一定程度量化椎体骨微结构。

关键词： X线计算机体层摄影纹理分析定量CT 骨密度