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Attenuation of dextran sodium sulphate induced colitis in matrix metalloproteinase-9 deficient mice

World Journal of Gastroenterology 2006年第40期12卷 6464-6472页

作者： Alfredo santana Carlos Medina Maria Cristina Paz-Cabrera Federico Díaz-Gonzalez Esther Farré Antonio Salas Marek W Radomski Enrique Quintero Gastroenterology Department and Research Unit Hospital Universitario de Canarias Tenerife Spain Reumathology Department Hospital Universitario de Canrias Tenerife Spain Pathology Department Hospital Mutua de Terrassa Barcelona Spain Institute of Molecular Medicine University of Texas Houston-Texas United States

AIM: To study whether matrix metalloproteinase-9 (MMP-9) is a key factor in epithelial damage in the dextran sodium sulphate (DSS) model of colitis in ***: MMP-9-deficient and wild-type (wt) mice were given 5% DSS in drinking water for 5 d followed by recovery up to 7 d. On d 5 and 12 after induction of colitis, gelatinases, MMP-2 and MMP-9, were measured in homogenates of colonic tissue by zymography and Western blot, whereas tissue inhibitor of metalloproteinases (TIMPs) were measured by reverse zymography. The gelatinolytic activity was also determined in supernatants of polymorphonuclear leukocytes (PMN) isolated from mice blood. Moreover, intestinal epithelial cells were stimulated with TNF-α to study whether these cells were able to produce MMPs. Finally, colonic mucosal lesions were measured by microscopic examination. RESULTS: On d 5 of colitis, the activity of MMP-9 was increased in homogenates of colonic tissues (0.24 ± 0.1 vs 21.3 ± 6.4, P < 0.05) and PMN from peripheral blood in wt (0.5 ± 0.1 vs 10.4 ± 0.7, P < 0.05), but not in MMP-9-deficient animals. The MMP-9 activity was also up-regulated by TNF-α in epithelial intestinal cells (2.5 ± 0.5 vs 14.7 ± 3.0, P < 0.05). Although colitis also led to increase of TIMP-1 activity, the MMP-9/TIMP-1 balance remained elevated. Finally, in the MMP-9-deficient colitic mice both the extent and severity of intestinal epithelialinjury were significantly attenuated when compared with wt mice. CONCLUSION: We conclude that DSS induced colitis is markedly attenuated in animals lacking MMP-9. This suggests that intestinal injury induced by DSS is modu-lated by MMP-9 and that inhibition of this gelatinase may reduce inflammation.

关键词： Matrix metalloproteinases MMP-9-deficient Dextran sodium sulphate Inflammatory bowel disease Experimental colitis

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Streptococcus agalactiae:Identification methods,antimicrobial susceptibility, and resistance genes in pregnant women

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World Journal of Clinical Cases 2020年第18期8卷 3988-3998页

作者： Fabrícia Almeida Fernandes santana Tais Viana Ledo de Oliveira Marcelo Barreto de Souza Filho Lucas santana Coelho da Silva Breno Bittencourt de Brito Fabrício Freire de Melo Cláudio Lima Souza Lucas Miranda Marques Márcio Vasconcelos Oliveira Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil Laboratório Oliveira Ltda LABOVitória da Conquista 45020740BahiaBrazil Campus Soane Nazaréde Andrade Universidade Estadual de Santa CruzIlhéus 45662900BahiaBrazil

BACKGROUND Group B Streptococcus(GBS)is a normal component of the gastrointestinal and genital microbiota in humans and can lead to important infections in *** To compare GBS isolation and identification methods as well as to assess the antibiotic susceptibility and to identify resistance genes in GBS strains from pregnant women attended in healthcare services from the city of Vitória da Conquista,in Bahia State,*** From January 2017 to February 2018,vaginorectal swabs were obtained from 186 participants and the samples were seeded onto chromogenic agar for GBS before and after inoculation in selective *** identification using 3 CAMP and latex tests was performed in samples with GBS-suggestive ***,disk diffusion antibiograms were performed in GBS-positive samples,and the detection of the resistance genes ermB,ermTR,mefA,and linB in the clindamycin and/or erythromycin-resistant samples was carried *** Thirty-two samples(17.2%)were *** culture in chromogenic agar after sample incubation in selective broth was the most sensitive method(96.9%)for GBS *** isolates were susceptible to penicillin,ampicillin,cefotaxime,and *** resistance was observed in 6 samples(18.8%),while 8 samples(25%)were *** erythromycin and/or clindamycin-resistant GBS strains had negative *** strains(25%)presented an M phenotype and 6 isolates(75%)presented a cMLSB *** ermB gene was identified in 4 samples(44.4%),the mefA gene was also found in 4 samples(44.4%),the ermTR gene was identified in 1 isolate(11.1%),and the linB gene was not found in any *** This study evidenced that the screening for SGB can be performed by means of various methods,including chromogenic media,and that the chemoprophylaxis for pregnant women who cannot use penicillin must be susceptibility-guided.

关键词： Streptococcus agalactiae Pregnancy Antimicrobial susceptibility

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3D printed millireactors for process intensification

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Chinese Journal of Chemical Engineering 2020年第1期28卷 180-190页

作者： Harrson S.santana Alan C.Rodrigues Mariana G.M.Lopes Felipe N.Russo Joao L.Silva Jr Osvaldir P.Taranto School of Chemical Engineering University of CampinasBrazil Federal Institute of Education Science and Technology of South of Minas GeraisBrazil

The scope of the present research aims at demonstrating the 3D printing use in the manufacturing of microchannels for chemical process applications. A comparison among digital model processing applications for 3D print(slicers) and a print layer thickness analysis were performed. The 3D print fidelity was verified in several devices, including the microchannels’ printing with and without micromixer zones. In order to highlight the 3D print potential in Chemical Engineering, the biodiesel synthesis was also carried out in a millireactor manufactured by 3D printing. The millireactor operated under laminar flow regime with a total flow rate of 75.25 ml·min^-1(increment of about 130 times over traditional microdevices used for biodiesel production).The printed millireactor provided a maximum yield of Ethyl Esters of 73.51% at 40 ℃, ethanol:oil molar ratio of7 and catalyst concentration of 1.25 wt% and residence time about 10 s. As a result of flow rate increment attained in the millireactor, the number of required units for scaling-up the chemical processes is reduced. Using the approach described in the present research, anyone could produce their own millireactor for chemical process in a simple way with the aid of a 3D printer.

关键词： Process intensification 3D printer Millireactors Micromixers Microdevices Biodiesel

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Estimation of the impact of biomass burning based on regional transport of PM_(2.5) in the Colombian Caribbean

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Geoscience Frontiers 2022年第1期13卷 430-436页

作者： Jehison Bolaño-Truyol Ismael L.Schneider Heidis cano Cuadro Jorge D.Bolaño-Truyol Marcos L.S.Oliveira Department of Civil and Environmental Universidad de la CostaCUCCalle 58#55–66BarranquillaAtlánticoColombia Department of Productivity and Innovation Universidad de la CostaCUCCalle 58#55–66BarranquillaAtlánticoColombia Faculdade Meridional IMED 304 Passo FundoRS 99070–220Brazil Universidad de Lima Avenida Javier Prado Este 4600Santiago de Surco 1503Peru

Deterioration of air quality due to the increase in atmospheric emissions from biomass burning(BB)is one of the major environmental problems *** this study,we estimated the contributions of BB to PM_(2.5) concentrations in the municipalities of Soledad and Malambo located in the Colombian *** evaluation period ranged from February 24 to March 30,2018,a period with a high number of BB events recorded in the surroundings of the evaluated *** contribution of BB to the two sampling sites was estimated using the Hybrid Single-Particle Lagrangian Integrated Trajectory(HYSPLIT)dispersion model with forwarding trajectories from each of the burning points identified by satellite images(n=1089).The PM_(2.5) emissions were determined using the fire radiative power(FRP),obtained by remote-sensing data,and corresponded to the radiant energy released per time unit by burning *** average PM_(2.5) concentrations during the evaluation period were 19.91μg/m^(3) for Soledad and 22.44μg/m^(3) for *** average contribution of BB to these municipalities was 22.8%and 28.8%,*** methodology used in this study allowed to estimate the contribution of this important source without knowledge of a previous tracer of BB,thereby increasing the use of the proposed procedure *** information would enable the implementation of effective mitigation,thereby diminishing the adverse impact of PM_(2.5) on the health of the population.

关键词： Biomass burning Particulate matter HYSPLIT Dispersion model Remote sensing

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Food Safety and Fish Farming: Serious Issues for Brazil

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Food and Nutrition Sciences 2020年第2期11卷 123-152页

作者： Ludmilla santana Soares e Barros Center of Environmental and Biological Agrarian Sciences UFRB Cruz das Almas Brazil

Fish farming in Brazil has been growing, and a new branch that has been taking up space and evolving, every year, as a source of leisure, is fish-pay. Despite being a leisure practice, this type of fish farming has contributed to the development of Brazilian aquaculture. With the growth of aquaculture in the country, concerns about the quality of the product arise, both financially and microbiologically. Aquaculture has caused some environmental problems, such as the increase in the population growth of bacteria specific to the aquatic environment and the possible proliferation of microorganisms resistant to antibiotics due to the indiscriminate use of these drugs in nurseries. Inadequate water quality conditions can contaminate fish and favor the development of diarrheal infections due to the microbiological load present in the fish. This article aims to review the literature covering the general characteristics of fish, including aspects of nutritional importance, and mechanisms of deterioration and analysis of freshness.

关键词： Characteristics Deterioration Nutritional Importance

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A Passive Repression Mechanism that Hinders Synergic Transcriptional Activation by Heat Shock Factors Involved in Sunflower Seed Longevity

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Molecular Plant 2014年第1期7卷 256-259页

作者： Javier Tejedor-cano Rail Carranco Jose-Maria Personat Pilar Prieto-Dapena Concepcion Almoguera Jose Manuel Espinosa Juan Jordano Departamento de Biotecnologia Vegetal tnstituto de Recursos Naturalesy Agrobiologia de Sevilla Consejo Superior de Investigaciones Cientificas (CSiC) Seville Spain

Different genetic programs that are active during late stages of zygotic embryogenesis are involved in seed phenotypes as longevity, basal thermotolerance, and desiccation tolerance. In sunflower, Heat Shock Factor A9 （HaHSFA9） controls one of such programs： the HaHSFA9 program. Overexpression of HaHSFA9 in seeds of transgenic tobacco showed the involvement of this transcription factor in basal thermotolerance and longevity;

关键词： PROTEINS AUX/IAA DOMAIN

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The REMO Ocean Data Assimilation System into HYCOM(RODAS_H):General Description and Preliminary Results

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Atmospheric and Oceanic Science Letters 2014年第5期7卷 464-470页

作者： Clemente Augusto Souza TANAJURA Alex Novaes santana Davi MIGNAC Leonardo Nascimento LIMA Konstantin BELYAEV XIE Ji-Ping Physics Institute Federal University of Bahia (UFBA) Salvador 40170-280 Brazil Oceanographic Modeling and Observation Network (REMO) Center for Research in Geophysics and Geology Federal University of Bahia (UFBA) Salvador 40170-280 Brazil Ocean Sciences Department University of California Santa Cruz (UCSC) USA Graduate Program in Geophysics Federal University of Bahia (UFBA) Salvador Brazil Shirshov Institute of Oceanology Russian Academy of Sciences (SHIRSHOV/RAS) Moscow Russia Institute of Atmospheric Physics(IAP) Chinese Academy of Sciences (CAS)

The first version of the Brazilian Oceano- graphic Modeling and Observation Network （REMO） ocean data assimilation system into the Hybrid Coordi- nate Ocean Model （HYCOM）（RODAS H） has recently been constructed for research and operational purposes. The system is based on a multivariate Ensemble Optimal Interpolation （EnOI） scheme and considers the high fre- quency variability of the model error co-variance matrix. The EnOl can assimilate sea surface temperature （SST）, satellite along-track and gridded sea level anomalies （SLA）, and vertical profiles of temperature （T） and salinity （S） from Argo. The first observing system experiment was carried out over the Atlantic Ocean （78°S-50°N, 100°W-20°E） with HYCOM forced with atmospheric reanalysis from 1 January to 30 June 2010. Five integra- tions were performed, including the control run without assimilation. In the other four, different observations were assimilated： SST only （A SST）; Argo T-S profiles only （AArgo）; along-track SLA only （A_SLA）; and all data employed in the previous runs （A_All）. The A_SST, A_Argo, and A_SLA runs were very effective in improv- ing the representation of the assimilated variables, but they had relatively little impact on the variables that were not assimilated. In particular, only the assimilation of S was able to reduce the deviation of S with respect to ob- servations. Overall, the A_All run produced a good analy- sis by reducing the deviation of SST, T, and S with respect to the control run by 39%, 18%, and 30%, respectively, and by increasing the correlation of SLA by 81%.

关键词： ocean data assimilation ensemble optimalinterpolation observing system experiment HYCOM Atlantic Ocean

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Case Study: Analysis of the Physical Factors of Palestinian Bioclimate

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American Journal of Climate Change 2014年第2期3卷 223-231页

作者： Jehad M. H. Ighbareyeh A. cano-Ortiz E. cano Department of Animal and Plant Biology and Ecology University of Jaen Jaen Spain

This study analyses the physical factors of the Palestinian bioclimate, the mean monthly and annual temperature, mean monthly maximum temperature, and mean monthly minimum temperature using data from six weather stations from the Palestine Meteorological Department, recorded in two periods: The first period from 1969 to 1981, the second period from 1975 to 1995 (more than 32 years). Statistical tests included a bioclimatic analysis of Palestinian meteorological stations for the periods from 1969-1981 and 1975-1995 by using bioclimatic classification of the Earth of Rivas Martinez Salvador, with regard to thermicity index, compensated thermicity index, annual ombrothermic index, and simple continentality index. The bioclimate of Palestineis affected by various factors, such as the Jordanian-Syrian desert, and its natural geography and topography, among others as well as biodiversity. Annual ombrothermic index value ranging between 0.6 to 3.4 and simple continentality index was from 12.4 to 18.1. It is concluded that the occupied Palestinian territories belong to the arid, semiarid, dry, sub-humid and humid ombrotype, and the Inframediterranean, Thermomediterranean and Mesomediterranean bioclimatic belt.

关键词： Indices Biodiversity Bioclimatology Ombrotype Thermotypes

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Assessing the estrogenic potency in a Portuguese wastewater treatment plant using an integrated approach

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Journal of Environmental Sciences 2010年第10期22卷 1613-1622页

作者： Mário S.Diniz Rita Maurício Mira Petrovic Maria J.López De Alda Leonor Amaral Isabel Peres Damiá Barceló Fernando santana Universidade Nova de Lisboa Faculdade de Ciencias e TecnologiaREQUIMTEDep.de Quimica Universidade Nova de Lisboa Faculdade de Ciencias e TecnologiaDep.de Ciencias e Engenharia do Ambiente Department of Environmental Chemistry-IIQAB-CSIC c/Jordi Girona

The estrogenic potency of a wastewater treatment plant （WWTP） was evaluated using chemical and biological analyses, which showed that after the station treatment processes some of the selected endocrine disruptor compounds （EDCs） were still present in the treated effluent （e.g., bisphenol A, alkylphenols, estrone）. Thus, the most common endocrine EDCs were identified and quantified and the overall estrogenicity of the treated effluent assessed by integrating the results. Male goldfish （Carassius auratus） were used as biological indicators in a 28-day experiment. Vitellogenin （Vtg）, gonadosomatic and hepatosomatic indices, steroids （17β-estradiol and 11-ketotestosterone） and histopathology were biomarkers used in fish to evaluate WWTP treated effluent estrogenicity, in combination with instrumental analyses. The results showed a significant increase （P 〈 0.01） in plasma and liver Vtg, which were significantly correlated （r = 0.66; P 〈 0.01）. The gonadosmatic index was significantly （P 〈 0.01） reduced in exposed fish. The steroid analyses revealed significant elevations in 17β-estradiol and depressed 11-ketotestosterone concentrations. The histological examinations show changes in exposed fish gonads, such as regressed testes and in some cases （43% to 75%） the development of ovo-testis in fish exposed to 50% and 100% treated effluent.

关键词： Carassius auratus endocrine disruptors vitellogenin steroids intersex

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Time-dependent impact of a high-fat diet on the intestinal barrier of male mice

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World Journal of Methodology 2024年第1期14卷 114-123页

作者： Carolline Santos Miranda Daiana Araujo santana-Oliveira Isabela Lopes Vasques-Monteiro Nathan Soares Dantas-Miranda Jade Sancha de Oliveira Glauser Flavia Maria Silva-Veiga Vanessa Souza-Mello Department of Anatomy Rio de Janeiro State UniversityRio de Janeiro 20551030Brazil

BACKGROUND Excessive saturated fat intake compromises the integrity of the intestinal mucosa,leading to low-grade inflammation,impaired mucosal integrity,and increased intestinal permeability,resulting in the migration of lipopolysaccharide(LPS)to other *** To evaluate the chronic effects(at 10 and 16 wk)of a high-fat diet(HFD)(with 50%energy as fat)on the phylogenetic gut microbiota distribution and intestinal barrier structure and protection in C57BL/6 *** Forty adult male mice were divided into four nutritional groups,where the letters refer to the type of diet(control and HFD or HF)and the numbers refer to the period(in weeks)of diet administration:Control diet for 10 wk,HFD for 10 wk,control diet for 16 wk,and HFD for 16 *** sacrifice,biochemical,molecular,and stereological analyses were *** The HF groups were overweight,had gut dysbiosis,had a progressive decrease in occludin immunostaining,and had increased LPS *** progression reduced the number of goblet cells per large intestine area and Mucin2 expression in the HF16 group,consistent with a completely disarranged intestinal ultrastructure after 16 wk of HFD *** Chronic HFD intake causes overweight,gut dysbiosis,and morphological and functional alterations of the intestinal barrier after 10 or 16 ***-dependent reductions in goblet cell numerical density and mucus production have emerged as targets for countering obesity-driven intestinal damage.

关键词： High-fat diet Intestine Ultrastructure Goblet cells Gut microbiota

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