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Journal of Integrative Plant Biology 2021年第7期63卷 1353-1366页

作者： zengxiu feng Hongyong Shi Minghui Lv Yuang Ma Jia Li Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations School of Life SciencesLanzhou UniversityLanzhou 730000China

Brassinosteroids(BRs)are a group of steroidal phytohormones,playing critical roles in almost all physiological aspects during the life span of a plant.In Arabidopsis,BRs are perceived at the cell surface,triggering a reversible phosphorylation-based signaling cascade that leads to the activation and nuclear accumulation of a family of transcription factors,represented by BES1 and BZR1.Protein famesylation is a type of post-translational modification,functioning in many important cellular processes.Previous studies demonstrated a role of famesylation in BR biosynthesis via regulating the endoplasmic reticulum localization of a key bassino-lide(BL)biosynthetic enzyme BR6ox2.Whether such a process is also involved in BR signaling is not understood.Here,we demonstrate that protein famesylation is involved in mediating BR signaling in Arabidopsis.A loss-of-function mutant of EN HANCED RESPONSE TO ABA 1(iERA1).encoding a(3 subunit of the protein famesyl transferase hoi oenzyme,can alter the BL sensitivity of bak1~4 from a reduced to a hypersensitive level,era 7 can partially rescue the BR defective phenotype of a hetero zygous mutant of bin2-1,a gain-of function mutant of BIN2 which encodes a negative regulator in the BR signaling.Our genetic and biochemical analyses revealed that ERA1 plays a significant role in regulating the protein stability of BES1.

关键词： Arabidopsis BES1 brassinolide brassinosteroids protein famesylation

TWISTED DWARF 1 Associates with BRASSINOSTEROID-INSENSITIVE 1 to Regulate Early Events of the Brassinosteroid Signaling Pathway

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Molecular Plant 2016年第4期9卷 582-592页

作者： Baolin Zhao Minghui Lv zengxiu feng Thomas Campbell Emmanuel Liscum Jia Li Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations School of Life Sciences Lanzhou University Lanzhou 730000 China Division of Biological Sciences University of Missouri Columbia MO 65211 USA C.S. Bond Life Sciences Center University of Missouri Columbia MO 65211 USA

A genome-wide screen for mutants showing altered brassinosteroid （BR） sensitivity or bril-like phenotypes resulted in the identification of two new mutant alleles of TWISTED DWARF 1 （TWD1）, twd1-4, and twdl-5. Pre- vious studies indicated that TWD1, also named as ULTRACURVATA 2 or FKBP42, associates with auxin efflux transporters and is essential for their biological functions. Although earlier reports showed that BR signaling is downregulated in twdl, how TWD1 is integrated in BR signaling has not been elucidated. Here, we provide ge- netic and biochemical evidence demonstrating that TWD1 interacts with the BR receptor BRI1 in vivo in a BR- independent manner. Further analyses indicated that TWD1 modulates the BR signal transduction not by altering ER quality control or protein abundance of BRI1; instead, TWD1 appears to be critical in BR- induced interaction of BRI1 and its co-receptor BAK1, as well as BR-induced phosphorylation of these two proteins. These results provide better understanding of the early events of the BR signaling pathway.

关键词： BAK1 brassinolide brassinosteroid BRI1 TWD1

ADS1 mediates brassinosteroids-induced auxin biosynthesis in Arabidopsis thaliana

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ADS1 mediates brassinosteroids-induced auxin biosynthesis in...

2018全国植物生物学大会

作者： Hongyong Shi zengxiu feng Liren Du Jingjie Zhang Dongzhi Zhang Jia Li Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations School of Life SciencesLanzhou University

A genome-wide activation tagging screen for genetic modifers of det2-1,a brassinosteroid(BR)-deficient mutant,led to the identification of adsl-D,a dominant suppressor of det2-l.A det 2-1 ads1-Ddouble mutant shows a significantly elongated leaf phenotype compared to det2-1.An ads1-D single mutant shows elongated leaf petiole,bend-down leaf tip and elongated hypocotyl relative to wild type Co1-0.Genetic analyses also indicated that ads1-Dcan partially suppress the defective phenotypes ofbri1-9 and bin.2-1.The expression patterns of ADS1 are similar to those of BIN2.Yeast two-hybrid,bimoleculer fluorescence complementation(BIFC),coimmunoprecipitation,and pull-down assays demonstrated that ADS1 can physically interact with BIN2 and its closest homologs,BILl and BIL2.In vitro phosphorylation assay indicated that ADS1 can be phosphorylated by BIN2.Although ads1-D can rescue weak BR signaling mutants,it cannot rescue the strong mutantbril-701 brllbrl3,suggesting that the function of ADS 1 depends on functional BR signaling.ADS1 expression levels were not significantly alteredeither after BL(the most active form of BRs) treatment or in a BR-deficient mutant,suggesting that ADS1 may not be transcriptionally regulated by BRs.In addition,our data indicated that BL can induce the accumulation of ADS1 in the nucleus.The ADS1-OXseedlings showed a phenotype similar to that of auxin overproducing mutants,whereasadsl-Cas9 exhibits a phenotype which is reminiscent of auxindeficient mutants.Therefore,ADS1 may be genetically related to an auxin signaling or biosynthetic pathway.Comparing the differentially expressed genes(DEGs) in ADS1-OXwith auxin-induced DEGs revealed that 466 of 835(55%) genes in ADS1-OX were co-regulated by IAA.Auxin biosynthetic genes,YUC3 and YUC7,were significantly upregulated in ADS1-OXand downregulated in adsl-Cas9.Similarly,YUC3 and YUC7 were significantly up-regulated in BRI1-OX and bin2-3 billbil2 and down-regulated in bri1-701 brllbrl3.In addition,BL treatment and significantly up-regulate YUC3 and YUC

关键词： Brassinosteroids ADS1 BIN2 Auxin YUCCA3 YUCCA7

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二次微波法制备纳米TiO_2的实验研究

太阳能学报 2010年第6期31卷 687-691页

作者：冯炜王一平邹克华金文涛翟增秀天津大学化工学院天津300072 天津市环境保护科学研究院国家环境保护恶臭污染控制重点实验室天津300191

使用二次微波辅助水热合成法,回避高温煅烧过程,在低温(473K)以下成功制备了锐钛矿型纳米晶体光催化剂TiO_2,样品表征结果证明该粉体样品粒径均匀,晶相单一,具有较大的比表面积和很高的光催化活性。

关键词：微波辅助法制备纳米晶体 TiO2 实验研究 MICROWAVE 水热合成法光催化活性样品锐钛矿型光催化剂煅烧过程表征结果比表面积证明粒径晶相回避高温粉体

纳米TiO_2光催化降解焦化废水的实验研究

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环境卫生工程 2011年第5期19卷 12-14页

作者：翟增秀邹克华冯炜天津市联合环保工程设计有限公司天津300191 天津市环境保护科学研究院国家环境保护恶臭污染控制重点实验室天津300191 天津大学化工学院天津300072

以焦化废水为研究对象,研究了在微波辅助的条件下通过水解法制备出平均粒径为4.27 nm的纳米级TiO2粒子光催化的效果及其影响因素,结果表明:UV/TiO2用于降解高浓度的焦化废水效果不是很理想,该粒子比较适用于焦化废水的深度处理。并确定... 详细信息

以焦化废水为研究对象,研究了在微波辅助的条件下通过水解法制备出平均粒径为4.27 nm的纳米级TiO2粒子光催化的效果及其影响因素,结果表明:UV/TiO2用于降解高浓度的焦化废水效果不是很理想,该粒子比较适用于焦化废水的深度处理。并确定了最佳的实验条件为反应时间2 h、TiO2投加量1 g/L、30%H2O2的投加量5 mL/L以及不需要调整废水pH。

关键词： TiO2 光催化焦化废水

微波辅助法制备负载型纳米TiO_2的实验研究

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环境卫生工程 2010年第6期18卷 7-10页

作者：邹克华翟增秀冯炜天津市环境保护科学研究院国家环境保护恶臭污染控制重点实验室天津300191 天津大学化工学院天津300072

以002×7型强酸性阳离子交换树脂为载体,采用微波辅助水热法制备了负载型纳米TiO2光催化剂。分别采用XRD、XPS、SEM、TEM等方法对所制备的样品进行了表征。以紫外灯为光源、甲基橙溶液为目标污染物研究了所制备产物的光催化活性。结果表... 详细信息

以002×7型强酸性阳离子交换树脂为载体,采用微波辅助水热法制备了负载型纳米TiO2光催化剂。分别采用XRD、XPS、SEM、TEM等方法对所制备的样品进行了表征。以紫外灯为光源、甲基橙溶液为目标污染物研究了所制备产物的光催化活性。结果表明:微波辅助法制备的负载型纳米TiO2样品经XRD分析均为锐钛矿相;TEM的分析结果显示,树脂表面负载的TiO2颗粒呈棒状结构,粒径为5～10 nm;样品的XPS分析进一步表明树脂表面负载了TiO2颗粒;光催化实验结果显示微波辅助法制备的负载型纳米TiO2经多次使用仍具有较高的光催化活性。

关键词：微波 TiO2 负载树脂

从国际著名引文数据库的比较分析看DISC的建设与发展

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数字图书馆论坛 2020年第7期 67-72页

作者：杨增秀张桂玲杨海超冯艳君赵卫华张欣机械工业信息研究院北京100037

本文选取Scopus、Web of Science、CiteSeerX等国际著名的引文数据库,从文献收录范围、元数据描述、检索功能、统计分析功能4个维度与国际科学引文数据库(Database of International Science Citation,DISC)进行对比分析,总结归纳当下... 详细信息

本文选取Scopus、Web of Science、CiteSeerX等国际著名的引文数据库,从文献收录范围、元数据描述、检索功能、统计分析功能4个维度与国际科学引文数据库(Database of International Science Citation,DISC)进行对比分析,总结归纳当下国际先进引文数据库在收录范围、检索途径、分析评价等方面所具有的特点,为进一步完善DISC的建设与服务工作提出相关建议。

关键词：引文数据库 Scopus Web of Science CiteSeerX DISC

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微波辅助萃取马齿苋抑菌活性成分的工艺优化

湖南农业科学 2015年第6期 70-73页

作者：曾秀桃马佳凤林颖朱晨王慧敏徐丽珊浙江师范大学化学与生命科学学院浙江金华321004

以马齿苋(Portulaca oleracea L.)为材料,以蒸馏水为萃取试剂,以对大肠杆菌的抑制活性为考察指标,通过单因素和正交试验优化了微波辅助萃取马齿苋抑菌活性成分的工艺。结果表明,各因素对马齿苋抑菌活性成分萃取的影响由大到小依次为:萃... 详细信息

以马齿苋(Portulaca oleracea L.)为材料,以蒸馏水为萃取试剂,以对大肠杆菌的抑制活性为考察指标,通过单因素和正交试验优化了微波辅助萃取马齿苋抑菌活性成分的工艺。结果表明,各因素对马齿苋抑菌活性成分萃取的影响由大到小依次为:萃取温度>微波功率>萃取时间>料液比,并得到最佳萃取工艺为微波功率800 W、萃取时间9 min、萃取温度50℃、料液比1︰50,在此工艺下制得马齿苋抑菌活性成分粗品的得率为15.94%。对细菌的抑制活性测定结果表明,马齿苋对沙门氏菌和枯草杆菌无抑制作用,但对金黄色葡萄球菌和大肠杆菌有抑制作用,最小抑制浓度(MIC)分别为6.25和1.56 mg/m L。

关键词：马齿苋抑菌活性微波辅助萃取工艺优化