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Acta Oceanologica Sinica 2011年第6期30卷 95-103页

作者： ZHU Yanbing LI Hebin ZHANG Xuqin ZHANG Chunyan XIANG Jionghua LIU Guangming School of Biotechnology Engineering Jimei University Xiamen 361021 China Xiamen Medical College Xiamen 361008 China

A thermostable superoxide dismutase （SOD） from the inshore thermophile Thermus sp. JM1.was purified to homogeneity by steps of fractional ammonium sulfate precipitation, DEAE-Sepharose chromatography and Phenyl-Sepharose chromatography. The specific activity of the purified native enzyme was 1.656 U/mg. A sod gene from this strain was cloned and overexpressed in Escherichia coli （E. coli）. The prepared apo-enzyme of the purified recombinant SOD （rSOD） was reconstituted with either Fe or Mn by means of incubation with appropriate metal salts. As a result, only Mn 2＋ - reconstituted rSOD （Mn-rSOD） exhibited the specific activity of 1.598 U/mg. SOD from Thermus sp. JM1.was Mn-SOD, judging by the specific activities analysis of Fe or Mn reconstituted rSODs and the insensitivity of the native SOD to both cyanide and H 2 O 2 . Both the native SOD and Mn- rSOD were determined to be homotetramers with monomeric molecular mass of 26 kDa and 27.5 kDa, respectively. They had high thermostability at 50 ° C and 60 ° C, and showed striking stability across a wide pH span from 4.0 to 1..0.

关键词： manganese superoxide dismutase thermostability purification reconstitution

2,6-二-O-戊基-β-环糊精涂渍Symmetry C_8柱拆分扁桃酸及其类似物对映体

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色谱 2004年第4期22卷 420-423页

作者：阮源萍张雪曼陈安齐邱海源黄培强厦门大学化学系

利用合成的 2 ,6 二 O 戊基 β 环糊精涂渍SymmetryC8色谱柱 ,研究了扁桃酸及其类似物等 6种外消旋对映体的反相高效液相色谱拆分。优化了色谱分离条件 ,探讨了扁桃酸的手性拆分机理。结果表明 ,采用优化后的甲醇水或甲醇 0 5 %... 详细信息

利用合成的 2 ,6 二 O 戊基 β 环糊精涂渍SymmetryC8色谱柱 ,研究了扁桃酸及其类似物等 6种外消旋对映体的反相高效液相色谱拆分。优化了色谱分离条件 ,探讨了扁桃酸的手性拆分机理。结果表明 ,采用优化后的甲醇水或甲醇 0 5 %三乙胺乙酸缓冲液流动相等色谱条件 ,扁桃酸、扁桃酸甲酯、苯基甘氨酸、苯基琥珀酸和安息香等 5种外消旋对映体达到或接近基线分离 ,其中前 4种对映体均为 (S) 构型先出峰。该法可用于实际样品的对映体纯度测定。

关键词：高效液相色谱法手性固定相 2,6-二-O-戊基-β-环糊精对映体分离手性分离扁桃酸

Effects of clinorotation on COL1.1. EGFP gene expression

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Science China(Life Sciences) 2004年第3期47卷 203-210页

作者： DAI Zhongquan1. LI Yinghui1. DING Bai1. ZHANG Yuguo2, LIU Weiquan2 & LIU Pengpeng2 1. Laboratory of Space Cellular and Molecular Biology, Institute of Space Medico-Engineering, Beijing 1.0094, China 2. College of Biological Science, Chinese Agriculture University, Beijing 1.0094, China 1. Laboratory of Space Cellular and Molecular Biology Institute of Space Medico-Engineering 100094 Beijing China 2. College of Biological Science Chinese Agriculture University 100094 Beijing China

Bone-formation related gene plays a critical role in bone loss induced by space mi-crogravity, however the exact mechanism is unclear. In this study, we aim to investigate the effect of microgravity on the activity of ?1.I) collagen (COL1.1. gene promoter and the expression of osteoblast-related genes. COL1.1.promoter was digested by restriction enzymes resulting in three DNA fragments. The fragments were ligated with the enhanced green fluorescent protein report gene, and subcloned into expression vectors. ROS1./2.8 cells transfected by these vec-tors were screened by G41., and enhanced green fluorescent protein (EGFP) positive colonies were isolated and cultured under clinostat condition. EGFP and Collagen type I expression level were detected by fluorescence intensity analysis and immunocytochemistry methods respectively. The results showed that the expression of EGFP and collagen type I was increased 24 h, 48 h after the cells were cultured under stimulated microgravity, illustrating that the activity of COL1.1.promoter might be increased. In conclusion, osteoblasts can compensatively increase the ex-pression of type I collagen by enhancing the activity of COL1.1.promoter under short-term simulated microgravity conditions.

关键词： simulated microgravity, osteoblast, gene expression, COL1.1 promoter.

Exogenous carbon acquisition of photosynthesis in Porphyra haitanensis (Bangiales, Rhodophyta) under emersed state

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Progress in Natural Science:Materials International 2004年第2期 42-48页

作者： ZOU Dinghui 1.* and GAO Kunshan 1.2 (1. Marine Biology Institute, Science Center, Shantou University, Guangdong 51.063, China 2. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China) Marine Biology Institute，Science Center，Shantou University，Guangdong 51.063，China Marine Biology Institute，Science Center，Shantou University，Guangdong 51.063，China Institute of Hydrobiology，Chinese Academy of Sciences，Wuhan 430072，China

The photosynthetic performances of Porphyra haitanensis thalli were investigated in order to understand its mechanisms for exogenous carbon acquisition during emersion at low tide. The emersed photosynthesis was studied by altering the pH value in the water film on the thalli surface, treating them with carbonic anhydarase inhibitors (acetazolamide and 6-ethoxyzolamide), adjusting the CO 2 concentrations in the air, and comparing the theoretical maximum CO 2 supply rates within the adherent water film with the observed photosynthetic CO 2 uptake rates. It was found that the principal exogenous inorganic carbon source for the photosynthesis of P. haitanensis during emersion was atmospheric CO 2. The driving force of CO 2 flux across the water film was the CO 2 concentration gradient within it. Carbonic anhydrase accelerated both extracellular and intracellular CO 2 transport. The emersed photosynthesis of P. haitanensis was limited by the present atmospheric CO 2 level, and would be enhanced by atmospheric CO 2 rise that would trigger global warming.

关键词： Porphyra haitanensis carbon dioxide photosynthesis emersion carbonic anhydrase.

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人类基因组的单核苷酸多态性及其研究进展

色谱 2003年第2期21卷 110-114页

作者：赵春霞石先哲吕申马坚妹杨青许国旺中国科学院大连化学物理研究所辽宁大连116011 大连医科大学辽宁大连116023

对单核苷酸多态性(SNP)的概念及特性作了简要说明,介绍了1.种检测SNP的方法,讨论了各种方法的原理及优缺点,并对目前SNP在遗传图的绘制、遗传病致病基因的搜寻、药物设计及法医学等方面的应用及其研究进展进行了综述。引用文献29篇。

关键词：人类基因组单核苷酸多态性检测技术医学应用综述 DNA序列

Principal Component-Discrimination Model and Its Application

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Transactions of Tianjin University 2004年第4期10卷 315-318页

作者：韩天锡魏雪丽蒋淳张玉琍 Tianjin University of Technology Tianjin 300191 China Tianjin Earthquake Bureau Tianjin 300201 China

Having researched for many years, seismologists in China presented about 80 earthquake prediction factors which reflected omen information of earthquake. How to concentrate the information that the 80 earthquake prediction factors have and how to choose the main factors to predict earthquakes precisely have become one of the topics in seismology. The model of principal component-discrimination consists of principal component analysis, correlation analysis, weighted method of principal factor coefficients and Mahalanobis distance discrimination analysis. This model combines the method of maximization earthquake prediction factor information with the weighted method of principal factor coefficients and correlation analysis to choose earthquake prediction variables, applying Mahalanobis distance discrimination to establishing earthquake prediction discrimination model. This model was applied to analyzing the earthquake data of Northern China area and obtained good prediction results.

关键词： principal component analysis discrimination analysis correlation analysis weighted method of principal factor coefficients

Temporal species distributions of planktonic protist communities in semi-enclosed mariculture waters and responses to environmental stress

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Acta Oceanologica Sinica 2010年第3期29卷 74-83页

作者： XU Henglong ZHU Mingzhuang JIANG Yong AL-RASHEID Khaled A S Laboratory of Protozoology Institute of Evolution and Marine Biodiversity Ocean University of China Qingdao 266003 China Zoology Department King Saud University Riyadh 11451 Saudi Arabia

In order to evaluate the relationships between temporal species succession of planktonic protists and physical-chemical parameters in semi-enclosed mariculture waters, species distributions in response to environmental stress were investigated in a shrimp-farming pond near Qingdao, China during a complete shrimp-culture cycle （May to October 2002）. A clear temporal succession in species distribution was found over the complete farming cycle. For example, before the introduction of the shrimp larvae there was low-variability of species distribution in the protist communities whereas during the stages immediately after, there was higher variability in species composition. Multiple linear/logistic regression analyses demonstrate that 1. protist taxa （e.g., Gyrodinium spirale, Teleaulax acuta, Prorocentrum spp. and Mesodinium pupula） were related to the nutrients, in particular ammonia and phosphates, alone or in combination with water temperature. These results suggest that temporal variations in species distribution of planktonic protist communities might be used in assessing water quality of semi-enclosed mariculture waters.

关键词： environmental stress mariculture planktonic protist microbial ecology

Several significant hydrographic characteristics and their formation mechanism in the South China Sea during the spring and summer of 1.98

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Acta Oceanologica Sinica 2003年第4期22卷 491-502页

作者： Xu Jianping 1.2,Chai Fei 2,3,Liu Zenghong 1.2,Zhu Bokang 1.2 1.Second Institute of Oceanography,State Oceanic Administration,Hangzhou 31.01.,China. 2.Key Laboratory of Ocean Dynamic Processes and Satellite Oceanography of State Oceanic Administration, Hangzhou 31.01., China 3.School of Marine Science, University of Maine, Orono, ME 04469-5741.USA State Ocean Adm Inst Oceanog 2 Hangzhou 310012 Peoples R China Kay Lab Ocean Dynam Proc Hangzhou 310012 Peoples R China Sattelite Oceanog & State Ocean Adm Hangzhou 310012 Peoples R China Univ Maine Sch Marine Sci Orono ME 04469 USA

dOn the basis of the CTD data obtained by the two cruises before and after summer monsoon burst (25th May, 1.98), several significant hydrographic characteristics, their variation and formation mechanism in the South China Sea (SCS) are analyzed. The results show that the Kuroshio water intrudes into the SCS in the form of loop current, and its main part is limited to the east of 1.8degreesE near the Luzon Strait. Then it continuously extends westwards under the influence of the large-scale circulation, and has a distinct impact on the area north of 1.degreesN. There are two high temperature and low salinity regions: west off Luzon and east off Vietnam respectively. One region west off Luzon strengthens obviously after summer monsoon burst, the other east off Vietnam shows a tendency of weakening in the north and strengthening in the south. The formation of these two high temperature and low salinity regions is related to the anti-cyclonic eddies existing in this area, besides, it is also related to the high temperature feature west of Luzon, the influx of Sulu Sea water and the influence of the large-scale air circulation. There is a cold region below the depth of 50 m in the south of Zhongsha Islands, and it strengthens after monsoon burst. The formation of cold water is related to the upwelling there.

关键词： monsoon burst hydrographic characteristics Kuroshio Luzon Strait South China Sea

Simulation study of phase retrieval for hard X-ray in-line phase contrast imaging

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Science China(Physics,Mechanics & Astronomy) 2005年第4期48卷 450-458页

作者： YU Bin1.2, PENG Xiang1.2, TIAN Jindong1. NIU Hanben1. DIAO Luhong3 & LI Hua3 1. Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education, Institute of Optoelec- tronics, Shenzhen University, Shenzhen 51.060, China 2. State Key Laboratory of Precision Measuring Technology and Instruments, Tianjin University, Tianjin 300072,China 3. Key Laboratory of Intelligent Information Processing, Institute of Computing Technology, Chinese Academy of Sciences, Beijing 1.0080,China 1. Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education Institute of Optoelectronics Shenzhen University 518060 Shenzhen China 2. State Key Laboratory of Precision Measuring Technology and Instruments Tianjin University 300072 Tianjin China 3. Key Laboratory of Intelligent Information Processing Institute of Computing Technology Chinese Academy of Sciences 100080 Beijing China

Two algorithms for the phase retrieval of hard X-ray in-line phase contrast imaging are presented. One is referred to as Iterative Angular Spectrum Algorithm (IASA) and the other is a hybrid algorithm that combines IASA with TIE (transport of intensity equation). The calculations of the algorithms are based on free space propagation of the angular spectrum. The new approaches are demonstrated with numerical simulations. Comparisons with other phase retrieval algorithms are also performed. It is shown that the phase retrieval method combining the IASA and TIE is a promising technique for the application of hard X-ray phase contrast imaging.

关键词： X-ray optics, phase contrast imaging, phase retrieval, angular spectrum, hard X-ray.

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杨联陞、李约瑟与《中国科学技术史》

科技导报 2019年第9期37卷 94-96页

作者：任增强山东大学国际汉学研究中心

1.54年,英国剑桥大学生物化学家李约瑟(Joseph Needham,1.00-1.95年)的皇皇巨著《中国科学技术史》(图1.的第一卷《导论》,由剑桥大学出版社出版发行。这套多卷本的著作,上迄中国上古时期,下至明清之交欧洲传教士来华,涉及科学思想史、... 详细信息

1.54年,英国剑桥大学生物化学家李约瑟(Joseph Needham,1.00-1.95年)的皇皇巨著《中国科学技术史》(图1.的第一卷《导论》,由剑桥大学出版社出版发行。这套多卷本的著作,上迄中国上古时期,下至明清之交欧洲传教士来华,涉及科学思想史、数学、天文学、物理学、化学、生物学等众多中国科技门类,又加之是出自外国学者之手,可谓是史无前例.故而《导论》卷甫一出版便引发了海内外学界的瞩目和评论。

关键词：《中国科学技术史》李约瑟英国剑桥大学生物化学家科学思想史上古时期中国科技出版社