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Construction of a regulable gene therapy vector targeting for hepatocellular carcinoma

World Journal of Gastroenterology 2003年第4期9卷 688-691页

作者： Shao-YingLu Yan-FangSui zeng-shanli Cheng-EnPan JingYe Wen-YongWang DepartmentofGeneralSurgery FirstHospitalofXi'anJiaotongUniversityXi'an710061ShaanxiProvinceChina DepartmentofPathology FourthMilitaryMedicalUniversityXi'an710032ShaanxiProvinceChina DepartmentofGeneralSurgery FirstHospitalofXi'anJiaotongUniversityXi'an710061ShaanxiProvinceChina

AIM: To construct a gene modified hepatocellular carcinoma (HCC) specific EGFP expression vector regulated by abbreviated cis-acting element of AFP ***: The minimal essential DNA segments of AFP gene enhancer and promoter were synthesized through PCR from Genome DNA of HepG2 cells. Gene fragments were then cloned into the multiple cloning site of non-promoter EGFP vector pEGFP-t. Recombinant plasmid was transferred into positive or negative AFP cell lines by means of lipofectamine. The expression of EGFP was tested by fluorescence microscope and flow cytometry. The effect of all-trans retinoic acid (ATRA) on the expression of EGFP was tested in different ***: By the methods of restriction digestion and sequence analyses we confirmed that the length, position and orientation of inserted genes of cis-acting element of AFP were all correct. The transcription of EGFP was under the control of AFP cis-acting element. The expressing EGFP can only been detected in AFP producing hepatoma *** expression rate of EGFP in G418 screened cell line was 34.9±4.1%. 48 h after adding 1×10-7M retinoic acid, EGFP expression rate was 14.7±3.5%. The activity of AFP gene promoter was significantly suppressed by addition of 1×10-7M retinoic acid (P＜0.05, P=0.003, t=6.488).CONCLUSION: This recombinant expression vector can be used as a gene therapy vector for HCC. The expression of tumor killing gene will be confined within the site of tumor and the activity of which can be regulated by retinoic acid.

关键词：肝细胞癌可控基因疗法载体聚合酶链反应 DNA扩增

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Superantigen-SEA gene modified tumor vaccine for hepatocellular carcinoma:An in vitro study

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World Journal of Gastroenterology 2004年第1期10卷 53-57页

作者： Shao-YingLu Yan-FangSui zeng-shanli Jing-Ye Hai-LongDong PingQu Xiu-MinZhang Yu-SongLi DepartmentofPathology FourthMilitaryMedicalUniversityXi'an710032ShaanxiProvinceChina DepartmentofPathology FourthMilitaryMedicalUniversityXi'an710032ShaanxiProvinceChina

AIM: To construct an eukaryotic superantigen gene expression vector containing the recombinant gene of SEA and CD80 molecule transmembrane region (CD80TM), and to expresss taphylococcus enterotoxin A (SEA) on the membrane of hepatocellular carcinoma (HCC) cell to form a superantigen gene modified tumor vaccine for HCC. METHODS: SEA and linker-CD80TM gene were amplified through PCR from plasmid containing cDNA of SEA and *** fragments were then subcloned into the multiple cloning sites of retroviral vector pLXSN. Recombinant plasmid was transferred into HepG2 cells mediated with lipofectamine,positive clones were selected in culture medium containing G418. RT-PCR and indirect immunofluorescence studies confirmed that SEA was expressed specifically on HCC cell membrane. INFT-ELISPOT study demonstrated that SEA protein was expressed on the membrane of HCC *** of HepG2-SEA primed CTLs (SEA-T) was analyzed by^51Cr release assay. T cells cultured with rhIL-2 (IL-2-T) were used as control. RESULTS: Restriction digestion and sequence analyses confirmed the correctness of length, position and orientation of inserted fusion genes. SEA was expressed on the surface of HepG2 cells, HepG2-SEA had strong stimulating effect on production of HepG2 specific CTL (P<0.001). SEA-T had enhanced cytotoxicity to HepG2 cells (P<0.05). CONCLUSION: Tumor cell membrane expressed superarfdgen can be used to reinforce the immune effect of tumor cell vaccine for HCC, which provides a new method of the enhanced active immunotherapy for HCC.

关键词：超抗原-SEA基因肿瘤疫苗肝细胞癌体内试验免疫疗法

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地塞米松联合地佐辛超前镇痛对外科术后镇痛效果的Meta-分析

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药物评价研究 2019年第4期42卷 768-774页

作者：陈瑞阳赵君涂志超曾珊丽湖北省孝感市中心医院药学部湖北孝感432100

目的系统评价地塞米松联合地佐辛超前镇痛用于外科术后镇痛效果和安全性,为临床应用提供循证医学证据。方法计算机检索PubMed、Embase、中国生物医学文献数据库(CBM)、中国知网(CNKI)、维普数据库(VIP)和万方数据库,收集地塞米松联合地... 详细信息

目的系统评价地塞米松联合地佐辛超前镇痛用于外科术后镇痛效果和安全性,为临床应用提供循证医学证据。方法计算机检索PubMed、Embase、中国生物医学文献数据库(CBM)、中国知网(CNKI)、维普数据库(VIP)和万方数据库,收集地塞米松联合地佐辛(试验组)和单用地佐辛(对照组)超前镇痛用于外科术后镇痛的随机对照研究,检索时限为从建库—2018年5月,提取有效数据后,应用RevMan 5.2软件进行Meta-分析。结果共纳入13项研究,893例患者。Meta-分析结果显示:试验组术后的VAS评分在2 h (MD=-0.63, 95%CI=-0.86～-0.41, P<0.01)、 4 h (MD=-0.31,95%CI=-0.41～-0.21,P<0.01)、8 h(MD=-0.85,95%CI=-1.08～-0.62,P<0.01)、12 h(MD=-0.89,95%CI=-1.05～-0.72,P<0.01)、24 h(MD=-1.16,95%CI=-1.49～-0.82,P<0.01)均小于对照组;镇痛药追加率(RR=0.08,95%CI=0.02～0.32,P<0.01)小于对照组;患者镇静满意度(RR=1.18,95%CI=1.07～1.31,P<0.01)高于对照组;恶心、呕吐发生率(RR=0.41,95%CI=0.30～0.56,P<0.01)低于对照组。结论地塞米松联合地佐辛具有良好的协同作用,可有效减轻术后疼痛,减少恶心呕吐发生,值得临床推广。

关键词：地塞米松地佐辛超前镇痛外科手术 Meta-分析

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A DERIVATIVE-HILBERT OPERATOR ACTING FROM LOGARITHMIC BLOCH SPACES TO BERGMAN SPACES

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Acta Mathematica Scientia 2024年第5期44卷 1916-1930页

作者： shanli YE Yun XU School of Science Zhejiang University of Science and TechnologyHangzhou310023China

Letμbe a positive Borel measure on the interval[0,1).The Hankel matrix■with entriesμn,k=μn+k,whereμn=■[0,1)tndμ(t),induces,formally,the operator■where■is an analytic function *** characterize the measuresμfor which■is bounded(resp.,compact)operator from the logarithmic Bloch space■into the Bergman space■,where 0≤α<∞,0

关键词： derivative-Hilbert operator logarithmic Bloch space Carleson measure

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Prediction of network public opinion based on bald eagle algorithm optimized radial basis function neural network

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International Journal of Intelligent Computing and Cybernetics 2022年第2期15卷 184-200页

作者： Jialiang Xie shanli Zhang Ling Lin College of Science Jimei UniversityXiamenChina

Purpose-In the new era of highly developed Internet information,the prediction of the development trend of network public opinion has a very important reference significance for monitoring and control of public opinion by relevant government ***/methodology/approach-Aiming at the complex and nonlinear characteristics of the network public opinion,considering the accuracy and stability of the applicable model,a network public opinion prediction model based on the bald eagle algorithm optimized radial basis function neural network(BES-RBF)is *** research is conducted with Baidu indexes such as“COVID-19”,“Winter Olympic Games”,“The 100th Anniversary of the Founding of the Party”and“Aerospace”as samples of network public ***-The experimental results show that the model proposed in this paper can better describe the development trend of different network public opinion information,has good stability in predictive performance and can provide a good decision-making reference for government public opinion control ***/value-A method for optimizing the central value,weight,width and other parameters of the radial basis function neural network with the bald eagle algorithm is given,and it is applied to network public opinion trend *** example verifies that the prediction algorithm has higher accuracy and better stability.

关键词： Network public opinion COVID-19 Bald eagle algorithm RBF neural network

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An accurate selectivity estimation method for window queries and an implementation thereof

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Geo-Spatial Information Science 2015年第2期18卷 81-89页

作者： Changxiu CHENG Jing YANG Xiaomei SONG shanli YANG Lijun WANG Academy of Disaster Reduction and Emergency Management Beijing Normal UniversityBeijing 100875China State Key Laboratory of Resources and Environmental Information Systems Institute of Geographic Science and Natural Resources ResearchChinese Academy of SciencesBeijing 100101China China Internet Network Information Center Beijing 100101China

Spatial selectivity estimation is crucial to choose the cheapest execution plan for a given query in a query *** article proposes an accurate spatial selectivity estimation method based on the cumulative density(CD)histograms,which can deal with any arbitrary spatial query *** this method,the selectivity can be estimated in original logic of the CD histogram,after the four corner values of a query window have been accurately interpolated on the continuous surface of the elevation *** the interpolation of any corner points,we first identify the cells that can affect the value of point(x,y)in the CD *** cells can be categorized into two classes:ones within the range from(0,0)to(x,y)and the other overlapping the range from(0,0)to(x,y).The values of the former class can be used directly,whereas we revise the values of any cells falling in the latter class by the number of vertices in the corresponding cell and the area ratio covered by the range from(0,0)to(x,y).This revision makes the estimation method more *** CD histograms and estimation method have been implemented in *** results show that the method can accurately estimate the selectivity of arbitrary query windows and can help the optimizer choose a cheaper query plan.

关键词： cumulative density(CD)histogram selectivity estimation window queries spatial database spatial query optimization

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A DERIVATIVE-HILBERT OPERATOR ACTING ON HARDY SPACES

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Acta Mathematica Scientia 2023年第6期43卷 2398-2412页

作者：叶善力冯光豪 School of Science Zhejiang University of Science and TechnologyHangzhou 310023China

Letμbe a positive Borel measure on the interval[0,1).The Hankel matrixHμ=(μn,k)n,k≥0 with entries μn,k=μn+k,whereμn=∫[0,1)tndμ(t),induces formally the operator asDHμ(f)(z)=∞∑n=0(∞∑k=0 μn,kak)z^(n),z∈D,where f(z)=∞∑n=0a_(n)z^(n) is an analytic function in *** characterize the positive Borel measures on[0,1)such thatDHμ(f)(z)=f[0,1)f(t)/(1-tz)^(2)dμ(t) for all f in the Hardy spaces Hp(0p and q≥1).We also study the analogous problem in the Hardy spaces H^(p)(1≤p≤2).

关键词： Derivative-Hilbert operators Hardy spaces Carleson measures

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Construction of van der Waals substrates for largely mismatched heteroepitaxy systems using first principles

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Science China(Physics,Mechanics & Astronomy) 2019年第12期62卷 89-95页

作者： ZhiMing Shi XiaoJuan Sun YuPing Jia XinKe Liu shanli Zhang ZhanBin Qi DaBing Li State Key Laboratory of Luminescence and Applications Changchun Institute of OpticsFine Mechanics and PhysicsChinese Academy of SciencesChangchun 130033China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

The high density of defects induced by large strains in largely mismatched heteroepitaxy systems, such as AlN and GaN, because of the large lattice and thermal mismatch between substrates and epilayers is the bottleneck problem. Graphene-assisted van der Waals(vdW) heteroepitaxy offers a new opportunity to resolve this problem. However, it suffers from the difficulty of nucleation. Here we theoretically assess the effects of five 2D materials for vdW heteroepitaxy of AlN and GaN, including graphene, hBN, MoS2, gC3N, and gC3N4, and provide physical insights using first-principle calculations. MoS2 and gC3N exhibit significant potential to overcome the shortcomings of graphene owing to their appropriate binding strengths and Al(or Ga)diffusion barriers. Moreover, the interface behavior between the epilayers and the substrates are carefully analyzed. Our findings are helpful not only for obtaining high-quality AlN and GaN films but also for developing new criterions to discover effective 2D materials for vdW heteroepitaxy.

关键词： van der Waals epitaxy 2D materials first principles

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ON　CONNECTIVITY　OF　THE　ESSENTIAL　SPECTRA　OF　TOEPLITZ　OPERATORS　WITH　SYMBOLS　IN　H∞＋C

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Chinese Annals of Mathematics,Series B 1995年第1期16卷 93-102页

作者： CAO GUANGFU SUN shanli(Department of Mathematics, Jinn University Changchun 130023, China.) JILIN UNIV DEPT MATHCHANGCHUN 130023PEOPLES R CHINA

This paper discusses the connectivity of the essential spectra of Toeplitz operators with symbols in H∞ +C on Hardy spaces and weighted Bergman spaces for several complex variables.

关键词： Hardy space Weighted Bergman space Toeplitz operator Essential spectrum.

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Differential physiological responses of the coastal cyanobacterium Synechococcus sp. PCC7002 to elevated pCO2 at lag, exponential,and stationary growth phases

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Science China Earth Sciences 2018年第10期61卷 1397-1405页

作者： shanli MOU Gang LI Hongmei LI Fazhong LI Zhisheng SHAO Jinsong LI Changfeng QU Yongyu ZHANG Key Laboratory of Biofuels Shandong Provincial Key Laboratory of Energy Genetics Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of SciencesQingdao 266101China Key Laboratory of Tropical Marine Bio-resources and Ecology South China Sea Institute of Oceanology Chinese Academy of SciencesGuangzhou 510301 China Zhanjiang Branch of China National Offshore Oil Corporation Ltd. Zhanjiang 524000 China Key Laboratory of Marine Bioactive Substance the First Institute of OceanographyState Oceanic AdministrationQingdao 266061 China

We studied the effects of expected end-of-the-century p CO_2(1000 ppm)on the photosynthetic performance of a coastal marine cyanobacterium Synechococcus ***7002 during the lag,exponential,and stationary growth *** p CO_2significantly stimulated growth,and enhanced the maximum cell density during the stationary *** ambient p CO_2conditions,the lag phase lasted for 6 days,while elevated p CO_2shortened the lag phase to two days and extended the exponential phase by four *** elevated p CO_2increased photosynthesis levels during the lag and exponential phases,but reduced them during the stationary ***,the elevated p CO_2reduced the saturated growth light(Ik)and increased the light utilization efficiency(α)during the exponential and stationary phases,and elevated the phycobilisome:chlorophyll a(Chl a)***,the elevated p CO_2reduced the particulate organic carbon(POC):Chl a and particulate organic nitrogen(PON):Chl a ratios during the lag and stationary phases,but enhanced them during the exponential ***,Synechococcus showed differential physiological responses to elevated p CO_2during different growth phases,thus providing insight into previous studies that focused on only the exponential phase,which may have biased the results relative to the effects of elevated p CO_2in ecology or aquaculture.

关键词： Elevated pCO2 Lag Exponential and stationary phases Photosynthetic performance Synechococcus

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