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Highly efficient and genotype-independent barley gene editing based on anther culture

Plant Communications 2021年第2期2卷 89-101页

作者： Yong Han Sue Broughton Li Liu Xiao-Qi Zhang jianbin zeng Xiaoyan He Chengdao Li Western Barley Genetics Alliance College of ScienceHealthEngineering and EducationMurdoch UniversityMurdochWA 6150Australia Department of Primary Industries and Regional Development 3 Baron-Hay CourtSouth PerthWA 6151Australia Western Australian State Agricultural Biotechnology Centre Murdoch UniversityMurdochWA 6150Australia College of Agronomy Qingdao Agricultural UniversityQingdaoShandong 266109China

Recalcitrance to tissue culture and genetic transformation is the major bottleneck for gene manipulation in *** barley,immature embryos of Golden Promise have typically been used as explants for ***,the genotype dependence of this approach limits the genetic modification of commercial ***,we developed an anther culture-based system that permits the effective creation of transgenic and gene-edited plants from commercial barley *** protocol was tested in Golden Promise and four Australian varieties,which differed in phenology,callus induction,and green plant regeneration ***-mediated transformation was performed on microspore-derived callus to target the HvPDS gene,and T0 albinos with targeted mutations were successfully obtained from commercial *** editing of three targets was achieved with an average mutation rate of 53%in the five *** 51 analyzed T0 individuals,Cas9 induced a large proportion(69%)of single-base indels and two-base deletions in the target sites,with variable mutation rates among targets and *** ontarget and off-target activities were detected in T1 *** with immature embryo protocols,this genotype-independent platformcan deliver a high editing efficiency and more regenerant plants within a similar time *** shows promise for functional genomics and the application of CRISPR technologies for the precise improvement of commercial varieties.

关键词： Agrobacterium CRISPR genetic transformation Hordeum vulgare off-target targeted mutation

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Transcriptome Analysis Reveals a Novel High-Affinity Potassium Transporter HvHKT7 involved in Low Potassium in Barley(Hordeum vulgare L.)

Transcriptome Analysis Reveals a Novel High-Affinity Potassi...

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2014年中国作物学会学术年会

作者： jianbin zeng Department of Agronomy Key Laboratory of Crop Germplasm Resource of Zhejiang ProvinceZhejiang University

Potassium(K)deficiency is quite common and becomes more severe in crop growth and *** of low-K tolerant crops is an effective approach of overcoming the nutritional *** annual wild barley is rich in genetic diversity and can grow normally under poor soils,including low-K ***,the molecular mechanism underlying is still poorly *** the previous study,high-throughput Next Generation Sequencing(NGS)technique was employed to investigate

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Identification of proteins,Metabolites and Metabolic Pathway Associated with Low Potassium Tolerance in Barley

Identification of proteins,Metabolites and Metabolic Pathway...

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中国作物学会——2015年学术年会

作者： jianbin zeng Guoping Zhang College of Agriculture & Biotechnology Zhejiang University

In this study,TMT based high-throughput proteomic and GC-MS based metabolomics methods were performed on the two wild barley genotypes(XZ153,tolerant and XZ141,sensitive),and a low-K tolerance cultivar(Ludaomai),to investigate the genotypic difference in response to low-K *** 288differentially expressed proteins were identified between low-K and normal K treated *** them,129 proteins related to low-K tolerance were mainly involved in defense,transcription,signal transduction,energy,and protein *** proteomics results showed that XZ153 is higher capable of rearranging ion homeostasis and developing antioxidant defense system under low-K ***,ethylene response and phenylpropanoid pathways could determine the genotypic difference in low-K *** addition,a total of 61 key metabolites were identified and their concentrations were significantly affected by low potassium *** general,amino acids accumulation was enhanced in both roots and leaves,while TCA cycle was *** addition,the concentration of the negatively charged amino acids(Asp and Glu)and the negatively charged organic acid(Citric acid)were decreased in leaves and roots,but more positive charged amino acids(Lys)were increased in order to maintain the balance of charges in the ***,the probable physiological basis of barley responding to low potassium was the accumulation of glucose and other sugars by ensuring the energy ***153 showed increase in the content of active oxygen scavenger(such as proline and ascorbic acid)and the capacity of anti-oxidation stress,resulting in a higher low K *** current results confirmed the possibility of Tibetan wild barley in providing low-K tolerant genetic resource and identified some candidate proteins and metabolites for use in developing the cultivars with low-K tolerance.

关键词： Barley Low Potassium Tolerance Metabolic Pathway

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HYSTERESIS LOSSES AND THERMAL ANALYSIS OF GIANT MAGNETOSTRICTIVE HIGH POWER ULTRASONIC TRANSDUCER

HYSTERESIS LOSSES AND THERMAL ANALYSIS OF GIANT MAGNETOSTRIC...

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The 2011 International Conference on Mechanical Engineering and Technology

作者： zeng Haiquan zeng Gengxin zeng jianbin Yan Ming Department of Mechanical Engineering Xiamen University of Technology School of Mechanical and Automotive Engineering South China University of Technology Shenyang University of Technology

Giant magnetostrictive high power transducers usually work under a high intensity and frequency magnetic field. Hysteresis losses and eddy current losses are very heavy. At the same time, giant magnetostrictive materials（GGM） is sensitive to temperature. Therefore heat generation and temperature distribution is very important for the transducer’s design. In this paper a high power ultrasonic transducer was designed. The magnetization behavior of GGM in the transducer was characterized by the modified Jiles Atherton hysteresis model which considers eddy current losses and anomalous losses. Model parameters were determined from the least square fit between experimental values and model values. Various losses were calculated. Temperature distribution of the transducer was calculated with finite element method. Calculation results and experimental results show a good agreement.

关键词： high power ultrasonic transducer giant magnetostrictive materials(GGM) hysteresis loss finite element temperature analysis

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In silico curation of QTL-rich clusters and candidate gene identification for plant height of bread wheat

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The Crop Journal 2023年第5期11卷 1480-1490页

作者： Dengan Xu Chenfei Jia Xinru Lyu Tingzhi Yang Huimin Qin Yalin Wang Qianlin Hao Wenxing Liu Xuehuan Dai jianbin zeng Hongsheng Zhang Xianchun Xia Zhonghu He Shuanghe Cao Wujun Ma College of Agronomy Qingdao Agricultural UniversityQingdao 266109ShandongChina National Wheat Improvement Center Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijing 100081China

Many genetic loci for wheat plant height(PH) have been reported, and 26 dwarfing genes have been catalogued. To identify major and stable genetic loci for PH, here we thoroughly summarized these functionally or genetic verified dwarfing loci from QTL linkage analysis and genome-wide association study published from 2003 to 2022. A total of 332 QTL, 270 GWAS loci and 83 genes for PH were integrated onto chromosomes according to their locations in the IWGSC RefSeq v2.1 and 65 QTL-rich clusters(QRC) were defined. Candidate genes in each QRC were predicted based on IWGSC Annotation v2.1 and the information on functional validation of homologous genes in other species. A total of 38 candidate genes were predicted for 65 QRC including three GA2ox genes in QRC-4B-IV, QRC-5A-VIII and QRC-6A-II(Rht24) as well as GA 20-oxidase 2(TaSD1-3A) in QRC-3A-IV. These outcomes lay concrete foundations for mapbased cloning of wheat dwarfing genes and application in breeding.

关键词： QTL-rich clusters Plant height Semi-dwarfism Reduced height genes Candidate genes

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表面异物对252kV气体绝缘组合电器盆式绝缘子绝缘性能的影响

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电工技术学报 2019年第20期34卷 4208-4216页

作者：庄丞曾建斌袁传镇厦门理工学院电气工程与自动化学院厦门361000 许继(厦门)智能电力设备股份有限公司厦门361000

针对252kV气体绝缘组合电器(GIS)盆式绝缘子表面常见的五类异物(金属屑、毛发、纸屑、硅脂和沙粒)对其局部电场的影响建立模型,并利用三维有限元分析软件对其进行仿真计算。为验证理论计算的有效性,搭建一个盆式绝缘子绝缘试验平台。在... 详细信息

针对252kV气体绝缘组合电器(GIS)盆式绝缘子表面常见的五类异物(金属屑、毛发、纸屑、硅脂和沙粒)对其局部电场的影响建立模型,并利用三维有限元分析软件对其进行仿真计算。为验证理论计算的有效性,搭建一个盆式绝缘子绝缘试验平台。在此平台上,对异物附着在盆式绝缘子表面不同位置(高、中、低电位区)和不同排列方式(线性、片状)下进行雷电冲击、工频耐压及局部放大试验。试验结果表明:当体积很小的单一金属屑附着在绝缘子凸面高电位区时,未引起击穿现象;当金属屑位于绝缘子凸面低电位区时,片状排列较线性排列更容易引起击穿;四类非金属异物则对绝缘子的绝缘性能影响较小;试验中测得沙粒的局部放电量明显高于其他三类非金属异物,表明沙粒类异物长久积累后诱发绝缘故障的可能性会显著提高。

关键词：表面异物绝缘性能局部电场盆式绝缘子气体绝缘组合电器

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体育支教实习和集中实习的利弊分析研究

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闽南师范大学学报（自然科学版） 2017年第3期30卷 63-68页

作者：曾剑斌闽南师范大学体育学院福建漳州363000

为了探索体育实习的新途径,提升学生适应基础教育教学改革与社会发展的需要.采用文献资料法、问卷调查法、访谈法、观察法和数理统计法等,对闽南师范大学2012-2014级体育教育专业本科生教育实习状况进行调研,剖析体育支教实习和集中实... 详细信息

为了探索体育实习的新途径,提升学生适应基础教育教学改革与社会发展的需要.采用文献资料法、问卷调查法、访谈法、观察法和数理统计法等,对闽南师范大学2012-2014级体育教育专业本科生教育实习状况进行调研,剖析体育支教实习和集中实习模式的利弊,探寻解决体育实习中存在的问题,并就如何提高教育实习质量提出了相应的对策.

关键词：体育教育支教实习集中实习利弊

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高压交流输电线工频电磁场对人体头部的影响

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辐射研究与辐射工艺学报 2021年第4期39卷 72-83页

作者：龚赞陈志英曾建斌厦门理工学院电气工程与自动化学院厦门361024

高压交流输电线是产生工频电磁暴露环境问题的重要因素之一。为了研究高压交流输电线下人体头部周围电磁场的安全性,本研究以220 kV交流输电线为主要研究对象,利用COMSOL Multiphysics有限元软件建立交流输电线下的三维人体头部工频电... 详细信息

高压交流输电线是产生工频电磁暴露环境问题的重要因素之一。为了研究高压交流输电线下人体头部周围电磁场的安全性,本研究以220 kV交流输电线为主要研究对象,利用COMSOL Multiphysics有限元软件建立交流输电线下的三维人体头部工频电磁场有限元仿真模型,计算不同距离下人体头部的电磁场分布情况并判断其安全性。结果表明:头部形状的不同会影响电磁场的分布情况,头部的电磁场强度也与输电线之间的距离有关,输电线距离头部越远,其下方头部电磁场数值越小。根据国际非电离辐射防护委员会(International Commissionon Non-Ionizing Radiation Protection,ICNIRP)导则和GB 8702—2014标准,接地时头部电场强度最小安全距离分别为11.0 m和11.5 m;不接地时头部电场强度最小安全距离分别为10.5 m和12.0 m,头部磁通密度则均小于这两个标准限值。

关键词： 220 kV交流输电线工频电磁暴露有限元仿真三维人体头部模型

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汽轮机滑销系统卡涩原因分析与处理

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华电技术 2011年第1期33卷 18-20,30页

作者：曾健斌中国石化集团公司广州分公司广东广州510725

热电站汽轮发电机组滑销系统出现卡涩现象,造成了机组开、停过程胀差严重超标,危及机组设备和运行安全。对滑销系统卡涩部分的销槽进行了加工并重新配销,滑销系统卡涩现象基本消除。

关键词：汽轮机滑销系统卡涩胀差处理方法

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新形势下建筑工程造价的动态管理与控制

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城市建筑 2020年第18期17卷 195-196页

作者：曾剑斌广东天正工程咨询有限公司广东广州510000

随着我国的不断进步与发展,社会各界对于建筑工程事业又提出了新的要求,在新形势下,为了提高经营管理水平,实现自身经济效益的稳定增长,建筑工程企业的创新改革势在必行。造价管理是建筑工程企业管理的重要组成部分,加强造价管理工作水... 详细信息

随着我国的不断进步与发展,社会各界对于建筑工程事业又提出了新的要求,在新形势下,为了提高经营管理水平,实现自身经济效益的稳定增长,建筑工程企业的创新改革势在必行。造价管理是建筑工程企业管理的重要组成部分,加强造价管理工作水平,有助于提高工程资源利用率,节约工程成本,为企业可持续发展提供有效保障,因此,在造价管理工作的完善过程中,必须提高工作的全面性与有效性,本文针对新形势下建筑工程造价的动态管理与控制进行分析,以供参考。

关键词：建筑工程造价管理控制

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