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Identification of Differentially Expressed Genes During Anther Abortion of Taigu Genic Male Sterile Wheat by Combining Suppression Subtractive Hybridization and cDNA Array

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Journal of Integrative Plant Biology 2006年第11期48卷 1348-1354页

作者： Qing-Shan Chang Rong-Hua Zhou Xiu-Ying Kong zeng-Liang Yu ji-zeng jia Key Laboratory of Crop Germplasm and Biotechnology Ministry of Agriculture Institute of Crop Sciences Chinese Academy of Agricultural Sciences the National Key Facility for Crop Gene Resources and Genetic Improvement Beijing 100081 China Ion Beam Bioengineering Laboratory Institute of Plasma Physics the Chinese Academy of Sciences Hefei 230031 China

Talgu Genlc Male Sterile Wheat （TGMSW; Trltlcum aestlvum L.）, a dominant genic male sterile germplasm, is of considerable value in the genetic Improvement of wheat because of Its stable Inherence, complete male abortion, and high cross-fertilization rate. To Identify specially transcribed genes In sterile anther, a suppression subtractlve hybridization （aSH） library was constructed with sterile anther as the tester and fertile anther as the driver. A total of 2 304 SSH Inserts amplified by polymerase chain reaction were arrayed using robotic printing. The cDNA arrays were hybridized with 32P-labeled probes prepared from the RNA of forward- and reverse-subtracted anthers. Ninety-six clones were scored as upregulated in sterile anthers compared with the corresponding fertile anthers and some clones were selected for sequencing and analysis In GenBank. Based on their putative functions, 87 non-redundant clones were classified Into the following groups：（i） eight genes Involved In metabolic processes; （11） four material transportation genes; （iii） three signal transductlon-assoclated genes; （iv） four stress response and senescence-associated protein genes; （v） seven other functional protein genes; （vi） five genes with no known function; and （vii） another 56 genes with no match to the databases. To test the hybridization efficiency, eight genes were selected and analyzed by Northern blot. The results of the present study provide a comprehensive overview of the genes and gene products Involved In anther abortion In TGMSW.

关键词： anther cDNA array suppression subtractive hybridization Taigu Genic Male Sterile Wheat.

Cellular adaptation to hypoxia and p53 transcription regulation

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第5期10卷 404-410页

作者： Yang ZHAO Xue-qun CHEN ji-zeng DU Lab of Neurobiology and Physiology Department of Physiology School of Medicine Zhejiang University Hangzhou 310058 China

Tumor suppressor p53 is the most frequently mutated gene in human tumors. Meanwhile, under stress conditions, p53 also acts as a transcription factor, regulating the expression of a series of target genes to maintain the integrity of genome. The target genes of p53 can be classified into genes regulating cell cycle arrest, genes involved in apoptosis, and genes inhibiting angiogenesis, p53 protein contains a transactivation domain, a sequence-specific DNA binding domain, a tetramerization domain, a non-specific DNA binding domain that recognizes damaged DNA, and a later identified proline-rich domain. Under stress, p53 proteins accumulate and are activated through two mechanisms. One, involving ataxia telangiectasia-mutated protein （ATM）, is that the interaction between p53 and its down-regulation factor murine double minute 2 （MDM2） decreases, leading to p53 phosphorylation on Serl 5, as determined by the post-translational mechanism; the other holds that p53 increases and is activated through the binding of ribosomal protein L26 （RPL26） or nucleolin to p53 mRNA 5＇ untranslated region （UTR）, regulating p53 translation. Under hypoxia, p53 decreases transactivation and increases transrepression. The mutations outside the DNA binding domain of p53 also contribute to tumor progress, so further studies on p53 should also be focused on this direction. The subter- ranean blind mole rat Spalax in Israel is a good model for hypoxia-adaptation. The p53 of Spalax mutated in residue 172 and residue 207 from arginine to lysine, conferring it the ability to survive hypoxic conditions. This model indicates that p53 acts as a master gene of diversity formation during evolution.

关键词： p53 Transcription factor Hypoxia Adaptation

Compressed wormlike chain moving out of confined space: A model of DNA ejection from bacteriophage

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Acta Mechanica Sinica 2012年第4期28卷 1219-1226页

作者： ji-zeng Wang Long Li Hua-jian Gao Key Laboratory of Mechanics on Disaster and Environment in Western China Ministry of EducationSchool of Civil Engineering and MechanicsLanzhou UniversityLanzhou730000 GansuChina School of Engineering Brown UniversityProvidence02912 Rhode IslandUSA

The molecular biomechanics of DNA ejection from bacteriophage is of interest to not only fundamental biological understandings but also practical applications such as the design of advanced site-specific and controllable drug delivery systems. In this paper, we analyze the viscous motion of a semiflexible polymer chain coming out of a strongly confined space as a model to investigate the effects of various structure confinements and frictional resistances encountered during the DNA ejection process. The theoretically predicted relations between the ejection speed, ejection time, ejection length, and other physical parameters, such as the phage type, total genome length and ionic state of external buffer solutions, show excellent agreement with in vitro experimental observations in the literature.

关键词： DNA ejection Bacteriophage. Wormlike chain model Confined semi-flexible polymer. Drag coefficient

Allelic Variation in Loci for Adaptive Response and Its Effect on Agronomical Traits in Chinese Wheat(Triticum aestivum L.)

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Journal of Integrative Agriculture 2014年第7期13卷 1469-1476页

作者： GAO Li-feng LIU Pan GU Yan-chun jiA ji-zeng National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences Chinese Academy of Agricultural Sciences

Heading date was an important trait that decided the adaptation of wheat to environments. It was modiifed by genes involved in vernalization response, photoperiod response and development rate. In this study, four loci Xgwm261, Xgwm219, Xbarc23 and Ppd-D1 which were previously reported related to heading time were analyzed based on three groups of wheat including landraces （L）, varieties bred before 1983 （B82） and after 1983 （A83） collected from Chinese wheat growing areas. Generally, heading date of landrace was longer than that of varieties. Signiifcant differences in the heading time existed within the groups, which implied that diversiifcation selection was much helpful for adaptation in each wheat zone. Photoperiod insensitive allele Ppd-D1a was the ifrst choice for both landrace and modern varieties, which promoted the heading date about four days earlier than that of sensitive allele Ppd-D1b. The three SSR loci had different characters in the three groups. Predominant allele combination for each zone was predicted for wheat group L and A83, which made great contribution to advantageous traits. Xgwm219 was found to be signiifcantly associated with heading date in Yellow and Huai River Winter Wheat Zone （Zone II） and spike length in Middle and lower Yangtze Valley Winter Wheat Zone （Zone III）, which implied functional diversiifcation for adaption. Variation for earliness genes provided here will be helpful for whet breeding in future climatic change.

关键词： allele variation adaptation wheat (Triticum aestivum L.)

Impact of lymph node micrometastasis on gastric carcinoma prognosis:A meta-analysis

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World Journal of Gastroenterology 2015年第5期21卷 1628-1635页

作者： Yong-ji zeng Chun-Dong Zhang Dong-Qiu Dai Department of Gastrointestinal Surgery the Fourth Affiliated Hospital of China Medical University

AIM:To investigate the prognostic significance of lymph node micrometastasis(LNMM) in patients with gastric ***:Two reviewers independently searchedelectronic databases including Pub Med,EMBASE,the Cochrane Database of Systematic Reviews,the Cochrane Controlled Studies Register,and the China National Knowledge Infrastructure electronic database between January 1996 and January *** literature retrieval and data extraction were performed to extract relevant *** analysis was conducted using Rev Man 5.2.4 software,and relative risks(RRs) for patient death in five years and recurrence were calculated.A fixed- or random-effects model was selected to pool and a forest plot was used to display ***:Twelve cohort studies containing a total of 1684 patients were *** positivity was worse than LNMM negativity with regards to the number of patients who died in five *** effects of LNMM positivity in patients with gastric cancer of different T-stages remain *** in patients with gastric carcinoma was also associated with a higher recurrence *** regards to the number of patients who died in five years,Asian patients were worse than European and Australian ***:We recommend that LNMM should not be used as a gold standard for prognosis evaluation in patients with gastric cancer in clinical settings until more high quality trials are available.

关键词： Gastric carcinoma Survival Lymph node micrometasta

Regulation of Leaf Senescence and Crop Genetic Improvement

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Journal of Integrative Plant Biology 2012年第12期54卷 936-952页

作者： Xiao-Yuan Wu Ben-Ke Kuai ji-zeng jia Hai-Chun jing The Key Laboratory of Plant Resources Institute of BotanyChinese Academy of Sciences State Key Laboratory of Genetic Engineering and Institute of Plant Biology School of Life SciencesFudan University Institute of Crop Sciences Chinese Academy of Agricultural Sciences

Leaf senescence can impact crop production by either changing photosynthesis duration, or by modifying the nutrient remobiliza- tion efficiency and harvest index. The doubling of the grain yield in major cereals in the last 50 years was primarily achieved through the extension of photosynthesis duration and the increase in crop biomass partitioning, two things that are intrinsically coupled with leaf senescence. In this review, we consider the functionality of a leaf as a function of leaf age, and divide a leaf＇s life into three phases： the functionality increasing phase at the early growth stage, the full functionality phase, and the senescence and functionality decreasing phase. A genetic framework is proposed to describe gene actions at various checkpoints to regulate leaf development and senescence. Four categories of genes contribute to crop production： those which regulate （Ⅰ） the speed and transition of early leaf growth, （Ⅱ） photosynthesis rate, （Ⅲ） the onset and （Ⅳ） the progression of leaf senescence. Current advances in isolating and characterizing senescence regulatory genes are discussed in the leaf aging and crop production context. We argue that the breeding of crops with leaf senescence ideotypes should be an essential part of further crop genetic improvement.

关键词： Leaf Senescence photosynthesis and photoassimilates nutrient remobilisation senescence-associated genes crop ideotypes.

Shock Wave Solution for a Class of Nonlocal Nonlinear Singularly Perturbed Boundary Value Problems with Turning Point

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Acta Mathematicae Applicatae Sinica 2015年第3期31卷 701-706页

作者： Xiang-lin HAN Wan-tao LIN zeng-ji DU jia-qi MO Huzhou Teachers College State Key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamic Institute of Atmospheric Physics Chinese Academy of Sciences School of Mathematical Sciences Xuzhou Normal University Department of Mathematics Anhui Normal University

The nonlocal nonlinear boundary value problem with turning point is considered. Leading the stretched variables the formal asymptotic solution of the original problem is constructed. And by using the theory of differential inequalities the existence and uniformly validity of solution for the original boundary value problems are proved. The contribution of this paper is that, the asymptotic behavior of solution is studied by using a simple and special method.

关键词： turning point nonlocal nonlinear singular perturbation shock wave

A new method for position-sensitive measurement of beta surface contamination

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Nuclear Science and Techniques 2017年第2期28卷 49-56页

作者： Yan-Tao Qu Hui Wang Yang Liu Ling Chen jie Hou Zhe Liu ji-zeng Ma Department of Radiation Protection China Institute of Atomic Energy Beijing 102413 China Nuclear and Radiation Safety Center Ministry of Environment Protection Beijing 100082 China Occupational Radiation Protection Unit Radiation Safety and Monitoring Section NSRW IAEA PO Box 1001400 Vienna Austria

In this paper, we propose a new method for position-sensitive measurement of beta surface contamination. With a position-sensitive detector of enlarged sensitive detection area, accurate information of the contamination distribution can be obtained. The positionsensitive detection is based on a large-area plastic scintillator and the wavelength shifting(WLS) fibers and adopts the ‘‘light center of gravity'' method. Optical transmission of the detector is simulated with a preliminary detector model, and feasibility of the detector design and measurement method is evaluated using an experiment *** simulation and experiment results at different beta-ray incident points on the scintillator surface show that there is a polynomial relationship between the average amplitude ratio of the output pulses from the two parallel WLS fibers in the same fiber layer and the relative distance from the incident point to the WLS fiber.

关键词： Beta surface contamination Position-sensitive measurement Wavelength shifting fibers Plastic scintillator

The Characterization and Geographical Distribution of the Genes Responsible for Vernalization Requirement in Chinese Bread Wheat

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Journal of Integrative Plant Biology 2009年第4期51卷 423-432页

作者： Qing-Ming Sun Rong-Hua Zhou Li-Feng Gao Guang-Yao Zhao ji-zeng jia College of Biological Sciences China Agricultural University Beijing 100094 China Department of Crop Molecular Biology Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 China

The frequency and distribution of the major vernalization requirement genes and their effects on growth habits were studied. Of the 551 bread wheat genotypes tested, seven allelic combinations of the three Vrn-1 genes were found to be responsible for the spring habit, three for the facultative habit and one for the winter habit. The three Vrn-1 genes behaved additiveiy with the dominant allele of Vrn-A1 exerting the strongest effect. The allele combinations of the facultative genotypes and the discovery of spring genotypes with ＂winter＂ allele of Vrn-1 implied the presence of as yet unidentified alleles/genes for vernalization response. The dominant alleles of the three Vrn-1 genes were found in all ten ecological regions where wheat is cultivated in China, with Vrn-D1 as the most common allele in nine and Vrn-A1 in one. The combination of vrn-A1vrn-B1Vrn-D1 was the predominant genotype in seven of the regions. Compared with landraces, improved varieties contain a higher proportion of the spring type. This was attributed by a higher frequency of the dominant Vrn-A1 and Vrn-B1 alleles in the latter, Correlations between Vrn-1 allelic constitutions and heading date, spike length, plant type as well as cold tolerance were established.

关键词： common wheat genotyping geographical distribution vernalization.