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Genome-wide identification, classification, and expression profiling of serine esterases and other esterase-related proteins in the tobacco hornworm, Manduca sexta

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Insect Science 2023年第2期30卷 338-350页

作者： zelong miao Chao Xiong Xiaolong Cao Tisheng Shan Qiao Jin Haobo Jiang Department of Entomology and Plant Pathology Oklahoma State UniversityOklahomaStillwaterUSA

Serine esterases(SEs)are hydrolases that catalyze the conversion of carboxylic esters into acids and *** and carboxylesterases constitute two major groups of *** over a hundred of insect genomes are known,systematic identification and classification of SEs are rarely performed,likely due to large size and complex composition of the gene family in each *** their key roles in lipid metabolism and other physiological processes,we have categorized 144 *** SEs and SE homologs(SEHs),114 of which contain a motif of *** sequence alignment and phylogenetic tree analysis have revealed 39 neutral lipases(NLs),3 neutral lipase homologs(NLHs),11 acidic lipases(ALs),3 acidic lipase homologs(ALHs),a lipase-3,a triglyceride lipase,a monoglyceride lipase,a hormone-sensitive lipase,and a GDSL ***-three carboxylesterase genes encode 29α-esterases(AEs),12 AEHs(e.g.,SEH4-1–3),20 feruloyl esterases(FEs),2 FEHs,2β-esterases(BEs),2 integument esterases(IEs),1 IEH,4 juvenile hormone esterases,2 acetylcholinesterases,gliotactin,6 neuroligins,neurotactin,and an uncharacteristic esterase *** addition to these GXSXG proteins,we have identified 26 phospholipases and 13 *** profiling of these genes in specific tissues and stages has provided insights into their functions including digestion,detoxification,hormone processing,neurotransmission,reproduction,and developmental *** summary,we have established a framework of information on SEs and related proteins in *** to stimulate their research in the model species and comparative investigations in agricultural pests or disease vectors.

关键词： carboxylesterase esterase lipase phospholipase phylogenetic analysis RNA-Seq thioesterase

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The effect of a secretion-enhanced heavy chain on improving intein-based dual-vector co-delivery of a full-length factor Ⅷ gene

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Chinese Science Bulletin 2011年第2期56卷 158-163页

作者： ZHU FuXiang YANG ShuDe LIU zelong miao Jing QU HuiGe CHI XiaoYan College of Life Sciences Ludong University Yantai 264025 China

Treatment of hemophilia A by gene therapy is adversely affected by inefficient FVIII secretion and the large FVIII gene,which is difficult to package in the promising adeno-associated virus (AAV) *** secretion of FVIII is caused mainly by inefficient secretion of its heavy ***,we have employed a protein splicing-based dual-vector to co-transfer a B-domain-deleted FVIII (BDD-FVIII) gene,suggesting that the light chain,covalently ligated to a co-expressed heavy chain can improve the secretion of spliced ***,its level of secretion was affected by inefficient secretion the heavy ***,we studied the effect of a mutant heavy chain with L303E/F309S substitutions,which enhance FVIII secretion on the heavy chain itself and spliced FVIII when using a protein splicing-based split-delivery of a full-length FVIII *** vectors expressing Ssp DnaB intein-fused mutant heavy and light chains were transiently co-transfected into cultured COS-7 cells.A spliced FVIII protein was seen in co-transfected cells by Western blot *** heavy chain was secreted by cells transfected with the mutant heavy chain gene alone at (39±11) ng/mL and this secretion increased to (123±13) ng/mL when cells were co-transfected with the light chain gene,which was greater than the secretion of wild-type heavy *** amount of spliced FVIII in the culture supernatant of co-transfected cells was (86±14) ng/mL,with an activity of (0.61±0.08) IU/mL,which was greater than that of wild-type FVIII co-transfected *** FVIII and bioactivity were also detected in the combined culture supernatant of cells individually transfected with mutant heavy and light chain gene at a higher level than that of combined wild-type heavy and light chain *** suggested that the heavy chain with improved secretion markedly increased the efficacy of protein splicing-based split delivery of the full-length FVIII gene using a *** result

关键词：分泌作用基因传递因子和内含肽轻链载体基础蛋白质剪接

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Why the abnormal phenomena of D-band center theory exist?A new BASED theory for surface catalysis and chemistry

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Chinese Journal of Catalysis 2024年第9期64卷 44-53页

作者： zelong Qiao Run Jiang Jimmy Yun Dapeng Cao State Key Laboratory of Organic‐Inorganic Composites Beijing University of Chemical TechnologyBeijing 100029China Qingdao International Academician Park Research Institute Qingdao 266000ShandongChina School of Chemical Engineering The University of New South WalesSydneyNSW 2052Australia

Since the D-band center theory was proposed,it has been widely used in the fields of surface chemistry by almost all researchers,due to its easy understanding,convenient operation and relative ***,with the continuous development of material systems and modification strategies,researchers have gradually found that D-band center theory is usually effective for large metal particle systems,but for small metal particle systems or semiconductors,such as single atom systems,the opposite conclusion to the D-band center theory is often *** solve the issue above,here we propose a bonding and anti-bonding orbitals stable electron intensity difference(BASED)theory for surface *** newly-proposed BASED theory can not only successfully explain the abnormal phenomena of D-band center theory,but also exhibits a higher accuracy for prediction of adsorption energy and bond length of intermediates on active ***,a new phenomenon of the spin transition state in the adsorption process is observed based on the BASED theory,where the active center atom usually yields an unstable high spin transition state to enhance its adsorption capability in the adsorption process of intermediates when their distance is about 2.5Å.In short,the BASED theory can be considered as a general principle to understand catalytic mechanism of intermediates on surfaces.

关键词： Surface chemistry Surface catalysis D-band center theory Bonding orbital Anti-bonding orbital

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Nano metal-photosensitizer based on Aza-BODIPY-Cu complex for CDT-enhanced dual phototherapy

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Chinese Chemical Letters 2024年第7期35卷 296-300页

作者： Wenjuan Jin zelong Chen Yi Wang Jiaxuan Li Jiahui Li Yuxin Pei Zhichao Pei College of Chemistry&Pharmacy Northwest A&F UniversityYangling 712100China

Chemodynamic therapy(CDT)combined with dual phototherapy(photothermal therapy(PTT)and photodynamic therapy(PDT))is an efficient way to synergistically improve anti-tumor ***,the combination of multiple modes often makes the composition of the system more complex,which is not conducive to clinical *** this study,a dual phototherapy ligand carboxyl-modified Aza-BODIPY(BOD-COOH)and metal active center Cu^(2+)were used to construct multiple-modes metalphotosensitizer nanoparticles(BOD-Cu NPs)via one-step coordination self-assembly for combination therapy of CDT/PDT/*** order to improve delivery efficiency,the targeted hydrophilic molecule pyridinemodified glucose derivative(G-Py)was synthesized and coated onto the BOD-Cu NPs to form a glycosylated nano metal-photosensitizer BOD-Cu@G by electrostatic *** Cu^(2+)in BOD-Cu@G could not only be used as a coordination node for metal-driven self-assembly but also consume intracellular glutathione(GSH),and then catalyze Fenton-like reaction to generate hydroxyl radical(·OH)for *** vitro and in vivo studies revealed that BOD-Cu@G could achieve excellent anti-tumor efficiency by CDTenhanced dual phototherapy.

关键词： Nano metal-photosensitizer Aza-BODIPY-Cu Coordination self-assembly Chemodynamic therapy Dual phototherapy

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The Ca^(2+)/CaN/ACC and cAMP/PKA/HK signal pathways are required for PBAN-mediated sex pheromone biosynthesis in Conogethes punctiferalis

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Journal of Integrative Agriculture 2024年第8期23卷 2735-2751页

作者： Yao Zhang zelong She Ruolan He Shuangyan Yao Xiang Li Xiaoguang Liu Xinming Yin Jizhen Wei Mengfang Du Shiheng An State Key Laboratory of Wheat and Maize Crop Science/Henan International Laboratory for Green Pest Control/College of Plant Protection Henan Agricultural UniversityZhengzhou 450046China

Conogethes punctiferalis is a crop and fruit pest that has caused serious economic losses to agricultural *** pest relies heavily on its sex pheromone to ensure sexual encounters and subsequent mating ***,the molecular mechanism underlying sex pheromone biosynthesis in this species remains *** present study investigated the detailed mechanism underlying PBAN-regulated sex pheromone biosynthesis in *** by transcriptome sequencing of the *** pheromone glands(PGs)and subsequent functional identification of the target *** results showed that female mating started from the first scotophase,and peaked at the second to fifth scotophases in accordance with the release of sex *** regulated sex pheromone biosynthesis by employing Ca^(2+)and cAMP as secondary messengers,as demonstrated by RNA interference(RNAi),pharmacological inhibitors,and behavioral *** investigation revealed that calcineurin(CaN)and acetyl-CoA carboxylase(ACC)were activated by PBAN/Ca^(2+)signaling,and the RNAimediated knockdown of CaN and ACC transcripts significantly reduced sex pheromone production,ultimately leading to a significantly reduced ability of females to attract ***,hexokinase(HK)was found to regulate sex pheromone biosynthesis in response to the PBAN/cAMP/PKA signaling pathway,as demonstrated by RNAi,enzyme activity,and pharmacological inhibitor ***,Far2 and Desaturase1 were found to participate in PBAN-regulated sex pheromone ***,our findings revealed that PBAN regulates sex pheromone biosynthesis through the PBANR/Ca^(2+)/CaN/ACC and PBANR/cAMP/PKA/HK pathways in ***,which enriches our comprehension of the details of sex pheromone biosynthesis in moths.

关键词： Conogethes punctiferalis sex pheromone cAMP/PKA calcineurin acetyl-CoA carboxylase hexokinase

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Tailoring the Microstructure and Mechanical Property of Mg-Zn Matrix Composite via the Addition of Al Element

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Acta Metallurgica Sinica(English Letters) 2024年第3期37卷 438-452页

作者： Chong Wang Fuyuan Liu Xuejian Wang Enyu Guo zelong Du Kunkun Deng Zongning Chen Huijun Kang Guohao Du Tongmin Wang Key Laboratory of Solidification Control and Digital Preparation Technology(Liaoning Province) School of Materials Science and EngineeringDalian University of TechnologyDalian 116024China School of Mechanical Engineering Guizhou UniversityGuiyang 550025China Ningbo Institute of Dalian University of Technology Ningbo 315000China Shanxi Key Laboratory of Advanced Magnesium-Based Materials College of Materials Science and EngineeringTaiyuan University of TechnologyTaiyuan 030024China Shanghai Synchrotron Radiation Facility Shanghai Advanced Research Institute CASShanghai 201204China

The semi-solid stir casting method is adopted to prepare 10 wt%SiC_(p)/Mg-6Zn-0.5Ca-xAl(x=0,1,3 and 5 wt%)composites,and the microstructure evolution and mechanical property of composites with various Al content are *** results show that the addition of 3 wt%Al improves the distribution of SiC_(p),whereas the SiC_(p) cluster occurs again with Al content greater than 3%.An abnormal phenomenon of twinning is observed in the cast composites in this *** SiC_(p)/Mg-6Zn-0.5Ca composite possesses the highest twin content of~23%,for which tension twins(TTW)and compression twins(CTW)account for~19%and~3%,*** CTW is only observed in ZXA600 *** addition of Al has an inhibiting effect for the generation and growth of *** content of twin decreases firstly and then increases with increase of Al *** lowest twin content is obtained as Al increases to 3 wt%.It is found the existence of twin is detrimental to the mechanical property of ***-cast SiC_(p)/Mg-6Zn-0.5Ca-3Al composite with the lowest twin content exhibits the optimal mechanical property of yield strength,ultimate tensile strength and elongation for 100 MPa,188 MPa and 4.4%,*** outstanding mechanical property is attributed to the uniform distribution of SiC_(p),the low twin content and the well-distributed fine second phases.

关键词： Mg matrix composite Synchrotron tomography Twinning Liquid melt processing Mechanical property

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Construction of Dynamic Alloy Interfaces for Uniform Li Deposition in Li-Metal Batteries

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Energy & Environmental Materials 2024年第3期7卷 68-75页

作者： Qingwen Li Yulu Liu Ziheng Zhang Jinjie Chen zelong Yang Qibo Deng Alexander V.Mumyatov Pavel A.Troshin Guang He Ning Hu Tianjin Key Laboratory of Advanced Functional Porous Materials Institute for New Energy Materials and Low-Carbon TechnologiesSchool of Materials Science and EngineeringTianjin University of Technology Tianneng Co.Ltd Key Laboratory of Hebei Province on Scale-span Intelligent Equipment Technology Tianjin Key Laboratory of Power Transmission and Safety Technology for New Energy Vehicles and School of Mechanical EngineeringHebei University of Technology Federal Research Center for Problems of Chemical Physics and Medicinal Chemistry Zhengzhou Research Institute of HIT Harbin Institute of Technology

It is well accepted that a lithiophilic interface can effectively regulate Li deposition behaviors,but the influence of the lithiophilic interface is gradually diminished upon continuous Li deposition that completely isolates Li from the lithiophilic ***,we perform in-depth studies on the creation of dynamic alloy interfaces upon Li deposition,arising from the exceptionally high diffusion coefficient of Hg in the amalgam solid *** a comparison,other metals such as Au,Ag,and Zn have typical diffusion coefficients of 10-20 orders of magnitude lower than that of Hg in the similar solid solution *** difference induces compact Li deposition pattern with an amalgam substrate even with a high areal capacity of55 mAh *** finding provides new insight into the rational design of Li anode substrate for the stable cycling of Li metal batteries.

关键词： diffusion coefficient dynamic alloy interfaces Li dendrites Li solid solution uniform Li deposition

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Universal,predominant exciton transfer in perovskite nanocrystal solids

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Science China Materials 2024年第9期67卷 2818-2827页

作者： Yuqing Luo Zhiya Dang Lingchen Meng zelong Chen Zihao Li Tongtong Lu Xiaobin Rao Shuyuan Zhao Qi Sun Pingqi Gao School of Materials Shenzhen Campus of Sun Yat-sen UniversityShenzhen518107China State Key Laboratory of Optoelectronic Materials and Technologies Sun Yat-sen UniversityGuangzhou510006China State Key Laboratory of Molecular Reaction Dynamics and the Dynamic Research Center for Energy and Environmental Materials Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian116023China

Perovskite nanocrystal(PNC)solids are promising materials for optoelectronic *** studies have shown that exciton diffusion in PNC solids occurs via alternate exciton hopping(EH)and photon recycling(PR).The energy disorder induced by the size distribution is a common factor in PNC solids,and the impact of this energy disorder on the exciton diffusion remains ***,we investigated the exciton diffusion in CsPbBr3 NC solids with a Gaussian size distribution of 11.2±6.8 nm via steady and time-resolved photoluminescence(PL)spectroscopy with multiple detection bands in transmission *** results indicated that exciton diffusion was controlled by a downhill transfer among the different energy sites through the disordered energy landscape,as confirmed by the accompanying low-temperature PL analysis.A detailed examination revealed that the acceptor distribution in tandem with the reabsorption coefficient determined the contribution of EH and PR to exciton transfer between different energy ***,the exciton diffusion mechanism varied in PNC solids of different thicknesses:in a thin solid with a thickness of several hundred nanometers,the exciton transfer was dominated by efficient EH and PR from the high-energy sites to the lower-energy sites;in a few-micrometer-thick solid,transfer from the medium-energy sites toward the lower-energy sites also became prominent and occurred mainly through *** findings enhance the understanding of the vital role that the acceptor distribution plays in the exciton diffusion process in PNC solids,providing important insights for optoelectronic applications based on PNC *** work also exploits the use of commonly available tools for in-depth exciton diffusion studies,which reveals the interior diffusion information that is usually hidden in surface sensitive PL imaging methods.

关键词： photoluminescence spectroscopy exciton diffusion exciton transfer perovskite nanocrystals photon recycling exciton hopping reabsorption

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日本沼虾蜕皮过程中头胸甲外骨骼超微结构的改变

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中国水产科学 2018年第2期25卷 301-307页

作者：杜娟张俊芳郑征帆黄亚龙苗泽龙宁黔冀河南师范大学生命科学学院新乡医学院基础医学院

采用HE染色及石蜡切片的扫描电镜观察方法,描述了日本沼虾(Macrobrachium nipponense)蜕皮间期(C期,intermolt stage)及新旧表皮更替变化剧烈的蜕皮前晚期(D4期,late premolt stage)和蜕皮后期(postmolt stage)A-B期头胸甲外骨骼的形态... 详细信息

采用HE染色及石蜡切片的扫描电镜观察方法,描述了日本沼虾(Macrobrachium nipponense)蜕皮间期(C期,intermolt stage)及新旧表皮更替变化剧烈的蜕皮前晚期(D4期,late premolt stage)和蜕皮后期(postmolt stage)A-B期头胸甲外骨骼的形态结构变化特点。结果显示,日本沼虾外骨骼分为上表皮、外表皮、内表皮三层,D4期出现新的上表皮和外表皮,B期出现新内表皮,外表皮强嗜碱性而内表皮弱嗜酸性。扫描电镜观察发现,C期头胸甲内、外表皮均为几丁质-蛋白质纤维构成的平行板层结构且板层内有发达的孔道系统(pore canals,pc),但内外表皮板层间排列紧密程度不同,外表皮板层切面边缘较整齐,结构致密,板层内pc大小均一、近似圆形,内表皮板层切面边缘粗糙,结构较疏松,板层内pc大小不等、多为梭形。在蜕皮前后,新外表皮结构变化显著,与C期相比,D4期新外表皮超微结构与旧内表皮结构相似,而在A期,外表皮超微结构由疏松变为致密,这可能与蜕皮后表皮的钙化相关。本研究旨为阐明沼虾的蜕皮机制提供基础资料。

关键词：日本沼虾外骨骼蜕皮周期 HE染色扫描电镜

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A statistical study on the correlation between sporadic Ca+ layer and Es in Beijing（40.5°N, 116°E）

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Earth and Planetary Physics 2024年第5期8卷 753-761页

作者： YuChang Xun ZhiShan Chen LiFang Du HaoRan Zheng Jing Jiao zelong Wang ShaoHua Gong GuoTao Yang College of Physics College of Electronic Information and Optical Engineering Taiyuan University of Technology State Key Laboratory of Space Weather National Space Science Center Chinese Academy of Sciences School of Science Jiangsu University of Science and Technology School of Physics and Electronics Engineering Hainan Normal University

From April 2020 to June 2022, a Ca+lidar with dual-wavelength and narrow-band emitting lasers utilizing all-solid-state technology was employed to conduct observations in Beijing（40.41°N, 116.01°E） for a total duration of 1797.79 hours over 280 nights. A total of 286 sporadic Ca+layers were observed, with heights ranging from 88 to 120 km and densities varying from 5.33 to 2200 cm-3. We simultaneously observed the ionosonde, located 28 km from the lidar, and found a correlation of 61.89% between the sporadic Ca+ layer and the sporadic E layer. When the sporadic Ca+layer has a downward phase trend, there is a correlation of 76.84%. By excluding the influence of ionosonde sensitivity, we focus only on calcium ions that have a density exceeding 140 cm-3, which exhibit a correlation of82.25%. Above 100 km, the correlation significantly increases, reaching approximately 90%. Furthermore, the correlation between the sporadic Ca+ layer and the sporadic E layer is particularly noticeable during the summer season, reaching around 90%. This phenomenon can be attributed to the variations in metal ions that occur during different seasons.

关键词： the Meridian Project lidar sporadic Ca+ layer sporadic E layer

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