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The microbial community,nutrient supply and crop yields differ along a potassium fertilizer gradient under wheat-maize double-cropping systems

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Journal of Integrative Agriculture 2024年第10期23卷 3592-3609页

作者： zeli li Fuli Fang liang Wu Feng Gao Mingyang li Benhang li Kaidi Wu Xiaomin Hu Shuo Wang Zhanbo Wei Qi Chen Min Zhang Zhiguang liu Shandong Provincial Key Laboratory of Water and Soil Conservation and Environmental Protection College of Resources andEnvironmentLinyi UniversityLinyi 276000China National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources College of Resources and theEnvironmentShandong Agricultural UniversityTai’an 271018China Key Laboratory of Crop Specific Fertilizer Ministry of Agriculture and Rural Affairs/Xinyangfeng Agricultural Technology Co.Ltd.Jingmen 448001China Institute of Applied Ecology Chinese Academy of SciencesShenyang 110000China

Soil microorganisms play critical roles in ecosystem ***,the relative impact of the potassium(K)fertilizer gradient on the microbial community in wheat-maize double-cropping systems remains *** this long-term field experiment(2008-2019),we researched bacterial and fungal diversity,composition,and community assemblage in the soil along a K fertilizer gradient in the wheat season(K0,no K fertilizer;K1,45 kg ha^(-1) K_(2)O;K_(2),90 kg ha^(-1)K_(2)O;K3,135 kg ha^(-1)K_(2)O)and in the maize season(K0,no K fertilizer;K_(1),150 kg ha^(-1) K_(2)O;K_(2),300 kg ha^(-1)K_(2)O;K_(3),450 kg ha^(-1)K_(2)O)using bacterial 16S rRNA and fungal internally transcribed spacer(ITS)*** observed that environmental variables,such as mean annual soil temperature(MAT)and precipitation,available K,ammonium,nitrate,and organic matter,impacted the soil bacterial and fungal communities,and their impacts varied with fertilizer treatments and crop ***,the relative abundance of bacteria involved in soil nutrient transformation(phylum Actinobacteria and class Alphaproteobacteria)in the wheat season was significantly increased compared to the maize season,and the optimal K fertilizer dosage(K2 treatment)boosted the relative bacterial abundance of soil nutrient transformation(genus Lactobacillus)and soil denitrification(phylum Proteobacteria)bacteria in the wheat *** abundance of the soil bacterial community promoting root growth and nutrient absorption(genus Herbaspirillum)in the maize season was improved compared to the wheat season,and the K2 treatment enhanced the bacterial abundance of soil nutrient transformation(genus MND1)and soil nitrogen cycling(genus Nitrospira)genera in the maize *** results indicated that the bacterial and fungal communities in the double-cropping system exhibited variable sensitivities and assembly mechanisms along a K fertilizer gradient,and microhabitats explained the largest amount of the variation in crop yields,a

关键词： potassium fertilizer gradient microbial community wheat-maize double cropping climate change yield

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基于流域模型与遥感解译方法的农业面源污染精细化源解析技术研究

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中国生态农业学报（中英文） 2024年第9期32卷 1534-1543页

作者：李琪云李继峰李泽利沈彦军刘夏中国科学院遗传与发育生物学研究所农业资源研究中心/中国科学院农业水资源重点实验室/河北省节水农业重点实验室石家庄050022 河北师范大学地理科学学院/河北省环境演变与生态建设实验室石家庄050024 天津市生态环境监测中心天津300191

农业面源污染源解析是面源污染防治的基础。农作物种植是农业面源污染源的重要组成,然而传统的面源污染源解析方法不能量化不同农作物对农业面源污染负荷的贡献,其解析精度也难以满足环境管理部门精细化管理的客观需求。本研究以天津于... 详细信息

农业面源污染源解析是面源污染防治的基础。农作物种植是农业面源污染源的重要组成,然而传统的面源污染源解析方法不能量化不同农作物对农业面源污染负荷的贡献,其解析精度也难以满足环境管理部门精细化管理的客观需求。本研究以天津于桥水库上游沙河流域为研究区,采用流域模型与遥感解译的方法,解析流域总磷负荷来源与贡献,旨在建立一种农业面源污染源精细化源解析技术。研究结果显示:基于Google Earth Engine(GEE)平台的农作物遥感总体分类精度在88%以上,Kappa系数均大于0.81,整体分类结果可信。沙河流域主要作物类型包括冬小麦-夏玉米、板栗、果树与其他作物,其中冬小麦-夏玉米的种植面积最大,占比为44%~67%,板栗种植面积次之,占比为11%~29%。冬小麦-夏玉米种植面积总体呈下降趋势,板栗种植面积则呈快速上升趋势。沙河流域Generalized Watershed Loading Function(GWLF)模型对溪流量与总磷负荷的模拟表现良好,其模型校准期与验证期的NSE在0.59以上,R^(2)在0.79以上。耕地为沙河流域最大的面源总磷负荷来源,占总磷负荷总量的61%;在耕地中,冬小麦-夏玉米总磷负荷占比最大(52%),板栗次之(20%);考虑到板栗种植面积近年来不断上升,未来沙河流域面源总磷负荷仍有升高的风险。

关键词：农业面源污染源解析遥感解译流域模型

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于桥水库浮游植物群落时空动态及影响因素分析

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农业资源与环境学报 2024年第1期41卷 125-137页

作者：高锴李泽利赵兴华张晓绪梅鹏蔚张震天津市生态环境监测中心天津300191 天津天滨瑞成环境技术工程有限公司天津300191 天津天滨同盛环境科技有限公司天津300191

为探究于桥水库浮游植物群落结构时间与空间变化及其与环境因子的相关性,本研究于2020年5—12月,对于桥水库5个采样点浮游植物群落结构及水体理化因子进行调查分析,运用浮游植物种类多样性指数及综合营养状态指数(Tli)对水体营养状态进... 详细信息

为探究于桥水库浮游植物群落结构时间与空间变化及其与环境因子的相关性,本研究于2020年5—12月,对于桥水库5个采样点浮游植物群落结构及水体理化因子进行调查分析,运用浮游植物种类多样性指数及综合营养状态指数(Tli)对水体营养状态进行评价,同时应用Pearson相关性分析探究影响浮游植物群落结构的主要环境因子。结果表明:于桥水库浮游植物共隶属于7门86属194种,以绿藻门、蓝藻门及硅藻门为主,7月种类数最多,12月最少。优势种共有17种,蓝藻门最多。于桥水库全年浮游植物平均丰度为1.35×10^(8)cells·L^(-1),年均生物量为67.73 mg·L^(-1),从浮游植物群落组成时间差异来看,蓝藻门丰度始终最高,占比为78.75%~95.17%。甲藻门、金藻门及裸藻门占比均较小,合计占比在0.10%~1.48%之间。空间分布上,库中心浮游植物丰度最高,水库入口三岔口最低。Tli评价结果表明,于桥水库2020年处于轻度富营养状态,且夏季评价结果较好。在空间上,库区北部东马坊评价结果较差,水库入口三岔口较好。Pearson相关性分析结果表明,水温、pH、总氮浓度、高锰酸盐指数及叶绿素a浓度是影响于桥水库浮游植物群落结构的重要因子。

关键词：于桥水库浮游植物群落结构多样性指标 Pearson相关性

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于桥水库4种经济鱼类肌肉营养成分分析与评价

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饲料工业 2024年第16期45卷 92-98页

作者：张志华张达娟李泽利卞少伟张树林天津农学院水产学院天津市水产生态及养殖重点实验室天津300392 天津市生态环境监测中心天津300191

为全面了解于桥水库鲤鱼(Cyprinus carpio)、鲢鱼(Hypophthalmichthys molitrix)、鲫鱼(Carassius auratus)和鳙鱼(Aristichthys nobilis)的营养成分特征,对4种鱼肌肉的水分、灰分、粗脂肪、粗蛋白、矿物质元素含量、氨基酸及脂肪酸组... 详细信息

为全面了解于桥水库鲤鱼(Cyprinus carpio)、鲢鱼(Hypophthalmichthys molitrix)、鲫鱼(Carassius auratus)和鳙鱼(Aristichthys nobilis)的营养成分特征,对4种鱼肌肉的水分、灰分、粗脂肪、粗蛋白、矿物质元素含量、氨基酸及脂肪酸组成进行分析与评价。结果表明,鲢鱼粗蛋白含量最高(16.13%),鲫鱼含量最低(15.00%);鲢鱼粗脂肪含量最高(1.60%),鲫鱼含量最低(0.70%)。4种鱼富含矿物质K、Na、Ca、Mg、P及Fe、Zn、Se,为优质的高钾低钠膳食来源。人体必需氨基酸含量均占氨基酸总量的37.54%以上,鲜味氨基酸占40.51%~41.04%,谷氨酸含量均为最高。鲤鱼、鲫鱼、鲢鱼和鳙鱼肌肉中分别检出11、10、16和15种脂肪酸,饱和脂肪酸、单不饱和脂肪酸和多不饱和脂肪酸相对含量差异较小。4种鱼蛋白质含量较高,富含16种氨基酸,矿物质元素和脂肪酸含量丰富,具有较高的营养价值和食用风味。

关键词：于桥水库鲤鱼鲫鱼鲢鱼鳙鱼营养成分

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Rational design of hydroxytricyanopyrrole-based probes with high affinity and rapid visualization for amyloid-β aggregates in vitro and in vivo

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Chinese Chemical Letters 2024年第5期35卷 290-295页

作者： Jiajia Lv Jie Gao Hongyu li zeli Yuan Nan Dong School of Chemistry and Chemical Engineering Guizhou UniversityGuiyang 550025China School of Pharmacy Zunyi Medical UniversityZunyi 563003China Guizhou International Scientific and Technological Cooperation Base for Medical Photo-Theranostics Technology and Innovative Drug Development Zunyi 563003China

As key biomarkers,amyloid-β(Aβ)plaques are frequently used to diagnose Alzheimer’s disease(AD).Although fluorescence imaging has proven to be effective in detecting these plaques,the gold standard probe thioflavin T(ThT),used for Aβaggregates,cannot be applied in vivo owing to its invasive ***,the development of novel fluorescent probes capable of identifying Aβplaques in situ is *** on the ThT structure,twoπ-conjugated heterocyclic D-π-A probes were designed bearing the hydroxytricyanopyrrole acceptor and N,N-dimethylaminophenyl *** probes exhibited red to near-infrared fluorescence emission(λ_(max)=732 nm),large Stokes shifts(>100 nm),exceptional signal-to-noise ratio,rapid response(<30 s),and high binding affinity(NT-HTCP=33.32 nmol/L;NF-HTCP=53.35 nmol/L)for Aβ*** the best candidate,NT-HTCP was used for in situ imaging of Aβplaques in AD mouse ***,in vivo research demonstrated that NT-HTCP could cross the blood-brain barrier and continue imaging the Aβplaques with a good signal-to-noise ***,the outcomes of the docking computations helped guide the development of the Aβ*** study expands the family of N,N-dimethylaminophenyl-based Aβ-sensitive fluorophores,with NTHTCP emerging as a highly promising imaging agent.

关键词： Amyloid-β Fluorescent probe Hydroxytricyanopyrrole Fluorescence imaging Alzheimer’s disease

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Enhanced energy-storage density and temperature stability of Pb_(0.89)La_(0.06)Sr_(0.05)(Zr_(0.95)Ti_(0.05))O_(3) anti-ferroelectric thin film capacitor

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Journal of Materiomics 2022年第1期8卷 239-246页

作者： Zhenhua Tang Songcheng Hu Dijie Yao zeli li Zhigang liu Xiaobin Guo Biao Lu Jingmin Fan Xin-Gui Tang Sheng-Guo Lu Ju Gao School of Physics and Optoelectric Engineering Guangdong University of TechnologyGuangzhou Higher Education Mega CenterGuangzhou510006China Guangdong Provincial Key Laboratory of Functional Soft Condensed Matter School of Materials and EnergyGuangdong University of TechnologyGuangzhou510006China School of Automation Guangdong University of TechnologyGuangzhou510006China Department of Physics The University of Hong KongPokfulam RoadHong KongChina

As the fundamental energy storage components in electronic systems,dielectric capacitors with high power densities were *** this work,the anti-ferroelectric Pb_(0.89)La_(0.06)Sr_(0.05)(Zr_(0.95)Ti_(0.05))O_(3)(PLSZT)ceramics and thin film capacitor were successfully fabricated by a solid-state reaction route and pulsed laser deposition method,*** ferroelectric,dielectric,energy-storage properties,and temperature stability of anti-ferroelectric PLSZT capacitor were investigated in *** compared with the PLSZT ceramic(energy storage density is 1.29 J/cm^(3) with an efficiency of 78.7%under 75 kV/cm),the anti-ferroelectric PLSZT thin film capacitors exhibited the enhanced energy storage density of 52.6 J/cm^(3) with efficiency of 67.7%under an electric field as high as 2068.9 kV/cm,and the enhanced energy-storage temperature stabilities from room temperature(RT)to more than 200℃ were demonstrated,and the oxygen defects mechanism and size effect were ***,the fast charging(~0.05 μs)and discharging(~0.15 μs)time were certified for the anti-ferroelectric PLSZT film *** findings broaden the horizon for PLSZT anti-ferroelectrics in high energy storage properties and show promising for manufacturing pulse power capacitor.

关键词： PLSZT anti-Ferroelectrics Pulsed laser deposition(PLD) Ferroelectric/dielectric properties Energy storage Temperature stability

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Emerging probing perspective of two-dimensional materials physics:terahertz emission spectroscopy

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light(Science & Applications) 2024年第7期13卷 1278-1298页

作者： Yifei Wu Yuqi Wang Di Bao Xiaonan Deng Simian Zhang lin Yu-chun Shengxian Ke Jianing liu Yingjie liu zeli Wang Pingren Ham Andrew Hanna Jiaming Pan Xinyue Hu Zhengcao li Ji Zhou Chen Wang State Key Laboratory of New Ceramics and Fine Processing Key Laboratory of Advanced Materials of Ministry of EducationSchool of Materials Science and EngineeringTsinghua University100084 BeijingChina Beijing Advanced Innovation Center for Integrated Circuits 100084 BeijingChina

Terahertz(THz)emission spectroscopy(TES)has emerged as a highly effective and versatile technique for investigating the photoelectric properties of diverse materials and nonlinear physical processes in the past few ***,research on two-dimensional(2D)materials has experienced substantial growth due to their atomically thin structures,exceptional mechanical and optoelectronic properties,and the potential for applications in flexible electronics,sensing,and ***,these materials offer advantages such as tunable bandgap,high carrier mobility,wideband optical absorption,and relatively short carrier *** applying TES to investigate the 2D materials,their interfaces and heterostructures,rich information about the interplay among photons,charges,phonons and spins can be unfolded,which provides fundamental understanding for future *** it is timely to review the nonlinear processes underlying THz emission in 2D materials including optical rectification,photon-drag,high-order harmonic generation and spin-to-charge conversion,showcasing the rich diversity of the TES employed to unravel the complex nature of these *** applications based on THz emissions,such as THz lasers,ultrafast imaging and biosensors,are also *** further,we analyzed the unique advantages of spintronic terahertz emitters and the future technological advancements in the development of new THz generation mechanisms leading to advanced THz sources characterized by wide bandwidth,high power and integration,suitable for industrial and commercial *** continuous advancement and integration of TES with the study of 2D materials and heterostructures promise to revolutionize research in different areas,including basic materials physics,novel optoelectronic devices,and chips for post-Moore’s era.

关键词： optoelectronic versatile exceptional

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澳大利亚SINO选矿厂运行调试问题分析

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矿山机械 2014年第8期42卷 105-109页

作者：李葆生李泽理洛阳矿山机械工程设计研究院有限责任公司河南洛阳471039 矿山重型装备国家重点实验室(中信重工机械股份有限公司) 河南洛阳471039

针对澳大利亚SINO铁矿选矿厂调试中出现的精矿产量、品位不达标,运转率低等问题,经分析认为,自磨回路循环负荷率偏高是影响工艺指标的关键因素;工程细节设计问题对运转率影响很大。通过减小顽石破碎粒度和旋流分级返砂量,平衡自磨-球磨... 详细信息

针对澳大利亚SINO铁矿选矿厂调试中出现的精矿产量、品位不达标,运转率低等问题,经分析认为,自磨回路循环负荷率偏高是影响工艺指标的关键因素;工程细节设计问题对运转率影响很大。通过减小顽石破碎粒度和旋流分级返砂量,平衡自磨-球磨处理负荷是工程整改的核心。最后对该项目的发展方向进行了预测,从项目策划和管理角度提出了海外选矿项目工程设计应注意的事项。

关键词： SINO 铁矿运行调试问题分析设计改进

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超声清洗石油污染海滨土的效果与影响因素

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化工学报 2011年第5期62卷 1448-1454页

作者：温军杰贾永刚陈欢林李泽利中国海洋大学环境科学与工程学院海洋环境与生态教育部重点实验室山东青岛266100 浙江大学材料与化工学院教育部膜材料与水处理工程中心浙江杭州310027

土壤的石油类有机污染物污染是近几年继农药和重金属之后的又一大污染。石油主要是由烷烃、环烷烃和芳香烃组成的混合物,具有致癌、致畸、致突变的潜在性。常规技术净化酸性的落地油泥的效果并不理想,而声化学处理以前就被认为是修复碳... 详细信息

土壤的石油类有机污染物污染是近几年继农药和重金属之后的又一大污染。石油主要是由烷烃、环烷烃和芳香烃组成的混合物,具有致癌、致畸、致突变的潜在性。常规技术净化酸性的落地油泥的效果并不理想,而声化学处理以前就被认为是修复碳氢化合物污染土的一个有效方法。基于此,本研究对石油污染的海滨土开展了以声化学为技术手段的修复实验。在本实验研究中,泥浆浓度、清洗时间、温度、泥浆pH值、投加碱性添加剂与微量自由基试剂H2O2等因素对石油烃从细粒海滨土中的解吸效果的影响得到考虑。根据实验结果,在采用声化学工艺净化海滨细粒土时,建议工艺操作参数控制在液固比6∶1、超声作用时间5 min、常温20℃、pH值9、Na2CO3的投加量为0.15%。另外,在处理过程中有必要采取措施及时避免清洗过程油泥造成的固结现象。

关键词：声化学清洗落地油泥海滨土影响因素

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Anti-EpCAM Antibody lipid Directly-encapsulated Iron Oxide Magnetic Nano-vesicles for Highly Efficient Capture of Circulating Tumor Cells

Anti-EpCAM Antibody Lipid Directly-encapsulated Iron Oxide M...

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2017年中国肿瘤标志物学术大会暨第十一届肿瘤标志物青年科学家论坛

作者： Xiaofei liang Kai Wang Yiping He Peiyong li Xiangdong Kang Fenfen Xiang Junping Ao Jingying Hu Bizhi Shi zeli li Jiefeng Gao Hebai Shen Sanjun Cai Junjie Peng State Key Laboratory of Oncogenes and Related Genes Shanghai Cancer InstituteRenji HospitalShanghai Jiaotong University School of Medicine Key Laboratory of Medical Molecular Virology of Ministries of Education and Health Shanghai Medical College and Institute of Medical MicrobiologyFudan University Department of Colorectal Surgery Fudan University Shanghai Cancer Center Department of endoscopy Fudan University Shanghai Cancer Center Rui Jin Hospital Shanghai Jiaotong University School of Medicine Putuo Hospital Shanghai University of Traditional Chinese Medicine Department of Oncology Shanghai Medical CollegeFudan University

The selection of methods for construction of antibody-modified magnetic beads greatly affects their ability to recognize and sort cancer cells. In contrast to the traditional method of modifying magnetic spheres with antibodies on the surface, we directly coated magnetic beads with a lipid derivative of antiepithelial cell adhesion molecule(EpCAM) antibody to create an antibody magnetic lipid nanovesicle(AMLVs) with a shell membrane structure, which is similar to a cell membrane. The AMLVs had a higher efficiency on capturing different EpCAM-expressing circulating tumor cells(CTCs). The interaction process and mechanism of AMLVs with different cancer cells were analyzed. Compared with the immunomagnetic beads( IMBs) in the Cellsearch system, the AMLVs captured more cancer cell types in blood(4.4±1.2-fold for AMLVs vs Cellsearch TM, n=5, Pliver cancer patients, which showed that the AMLVs could be effectively capture CTCs with a positive rate of 74.84%(238/318), 62.82%(49/78), 83.12%(64/77) and 89.09%(49/55), respectively. These results were consistent with the clinical diagnosis and pathological analysis of 55 colorectal and 18 breast cancer patients. The isolated CTCs could also be used for the Kras gene mutation test by the conventional PCR amplification method. The AMLVs platform provides a potential application in the CTCs analysis.

关键词： immunomagnetic beads cell isolation lipid magnetic vesicle Circulating tumor cells cancer detection

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