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MicroRNA-638 inhibits cell proliferation by targeting phospholipase D1 in human gastric carcinoma

Protein & Cell 2015年第9期6卷 680-688页

作者： Jiwei Zhang zehua bian Jialiang Zhou Mingxu Song Zhihui Liu Yuyang Feng Li Zhe Binbin Zhang Yuan Yin Zhaohui Huang Wuxi Oncology Institute The Affiliated Hospital of Jiangnan University Wuxi 214062 China Department of Radiation Oncology The Affiliated Hospital of Jiangnan University Wuxi 214062 China Fudan University Shanghai Cancer Center and Institutes of Biomedical Sciences Shanghai Medical CollegeFudan University Shanghai 200032 China

MicroRNAs （miRNAs） are a type of small non-coding RNAs that are often play important roles in carcinogene- sis, but the carcinogenic mechanism of miRNAs is still unclear. This study will investigate the function and the mechanism of miR-638 in carcinoma （GC）. The expres- sion of miR-638 in GC and the DNA copy number of miR- 638 were detected by real-time PCR. The effect of miR-638 on cell proliferation was measured by counting kit-8 assay. Different assays, including bioinformatics algo- rithms （TargetScan and miRanda）, luciferase report assay and Western blotting, were used to identify the target gene of miR-638 in GC. The expression of miR-638 target gene in clinical CRC tissues was also validated by immunohistochemical assay. From this research, we found that miR-638 was downregulated in GC tissues compared with corresponding noncancerous tissues （NCTs）, and the DNA copy number of miR-638 was lower in GC than NCTs, which may induce the corresponding downregulation of miR-638 in GC. Ectopic expression of miR-638 inhibited GC cell growth in vitro. Subsequently, we identified that PLD1 is the target gene of miR-638 in GC, and silencing PLD1 expression phenocopied the inhibitory effect of miR-638 on GC cell proliferation. Fur- thermore, we observed that PLD1 was overexpressed inGC tissues, and high expression of PLDt in GC predicted poor overall survival. In summary, we revealed that miR- 638 functions as a tumor suppressor in GC through inhibiting PLDI.

关键词： gastric cancer miR-638 cell proliferation,PLD1

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MiR-139-5p inhibits migration and invasion of colorectal cancer by downregulating AMFR and NOTCH1

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Protein & Cell 2014年第11期5卷 851-861页

作者： Mingxu Song Yuan Yin Jiwei Zhang Binbin Zhang zehua bian Chao Quan Leyuan Zhou Yaling Hu Qifeng Wang Shujuan Ni Bojian Fei Weili Wang Xiang Du Dong Hua Zhaohui Huang Wuxi Oncology Institute the Affiliated Hospital of Jiangnan University Wuxi 214062 China Department of Surgical Oncology the Affiliated Hospital of Jiangnan University Wuxi 214062 China Department of Radiation Oncology the Affiliated Hospital of Jiangnan University Wuxi 214062 China Department of Pathology Fudan University Shanghai Cancer Center Shanghai 200032 China

MicroRNAs （miRNAs） that exert function by posttran- scriptional suppression have recently brought insight in our understanding of the role of ***-coding RNAs in carcinogenesis and metastasis. In this study, we described the function and molecular mechanism of miR-139-5p in colorectal cancer （CRC） and its potential clinical application in CRC. We found that miR-139-5p was significantly downregulated in 73.8% CRC samples compared with adjacent noncancerous tissues （NCTs）, and decreased miR-139-5p was associated with poor prognosis. Functional analyses demonstrated that ectopic expression of miR-139-5p suppressed CRC cell migration and invasion in vitro and metastasis in vivo. Mechanistic investigations revealed that miR-139-5p suppress CRC cell invasion and metastasis by targeting AMFR and NOTCH1. Knockdown of the two genes phe- nocopied the inhibitory effect of miR-139-5p on CRC metastasis. Furthermore, the protein levels of the two genes were upregulated in CRC samples compared with NCTs, and inversely correlated with the miR-139-5p expression. Increased NOTCH1 protein expression was correlated with poor prognosis of CRC patients. Together, our data indicate that miR-139-5p is a potentialtumor suppressor and prognostic factor for CRC, and targeting miR-139-5p may repress the metastasis of CRC and improve survival.

关键词： miR-139-5p AMFR NOTCH1 colorectalcancer metastasis prognosis

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Correction to:MiR-139-5p inhibits migration and invasion of colorectal cancer by downregulating AMFR and NOTCH1

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Protein & Cell 2021年第8期12卷 667-669页

作者： Mingxu Song Yuan Yin Jiwei Zhang Binbin Zhang zehua bian Chao Quan Leyuan Zhou Yaiing Hu Qifeng Wang Shujuan Ni Bojian Fei Weiii Wang Xiang Du Dong Hua Zhaohui Huang Wuxi Oncology Institute the Affiliated Hospital of Jiangnan UniversityWuxi 214062China Department of Surgical Oncology the Affiliated Hospital of Jiangnan UniversityWuxi 214062China Department of Radiation Oncology the Affiliated Hospital of Jiangnan UniversityWuxi 214062China Department of Pathology Fudan University Shanghai Cancer CenterShanghai 200032China

CORRECTION TO:PROTEIN CELL(2014)5(11):851-861 HTTPS://***/10.1007/S13238-014-0093-5 In the original publication the display of Fig.1 is in *** correct Fig.1 is available in this correction.

关键词： invasion colorectal cancer

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长链非编码RNA-CASC11在结直肠癌中表达下调预示不良预后

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国际遗传学杂志 2015年第4期38卷 179-183页

作者：边泽华全超黄朝晖江南大学附属医院肿瘤研究所

目的本研究对人结直肠癌中长链非编码RNA-CASC11的表达情况进行检测,同时分析其表达水平与临床病理因素之间的关系,探讨CASC11在结直肠癌诊断、预后分析等方面的临床意义和价值。方法运用qRTPCR检测76对结直肠癌及癌旁正常组织中CASC11... 详细信息

目的本研究对人结直肠癌中长链非编码RNA-CASC11的表达情况进行检测,同时分析其表达水平与临床病理因素之间的关系,探讨CASC11在结直肠癌诊断、预后分析等方面的临床意义和价值。方法运用qRTPCR检测76对结直肠癌及癌旁正常组织中CASC11的表达水平。通过Logistic回归和Log-Rank方法分别分析其表达水平与临床病理因素和预后的关系。结果发现CASC11在结直肠癌组织中存在明显的表达下调(P=0.001),并且与肿瘤转移存在负相关(P=0.037,OR=0.321)。同时CASC11的低表达患者表现出更差的预后(P=0.017)。结论结直肠癌中CASC11的表达下调预示不良预后。

关键词：结直肠癌长链非编码RNA 肿瘤易感候选基因11

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微塑料过滤器|THE REBIRTH OF KAREZ

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设计 2021年第6期34卷 10-10页

作者： Yuxuan Cai zehua bian Yuxuan Dai 武汉轻工大学

此解决方案旨在解决灌溉暗渠(kariz)干涸的问题,解决方案在中国新疆吐鲁番盆地施行,利用吐鲁番地区日夜温差大的优势,利用MOF的材料和风力来凝结水并将其供应给灌溉暗渠。设备顶部有一个可折叠的地下水补充器,可确保从空气中收集水。该... 详细信息

此解决方案旨在解决灌溉暗渠(kariz)干涸的问题,解决方案在中国新疆吐鲁番盆地施行,利用吐鲁番地区日夜温差大的优势,利用MOF的材料和风力来凝结水并将其供应给灌溉暗渠。设备顶部有一个可折叠的地下水补充器,可确保从空气中收集水。该设计获得2020if设计新秀奖评委点评:由于水资源的短缺,这是一项得以广泛应用的伟大举措,灌溉暗渠的重生具有巩固这一概念的技术基础。

关键词：暗渠吐鲁番盆地微塑料吐鲁番地区水资源可折叠过滤器地下水

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Engineered DNase-inactive Cpf1 variants to improve targeting scope for base editing in ***

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Synthetic and Systems Biotechnology 2021年第4期6卷 326-334页

作者： zehua Chen Jinyuan Sun Ying Guan Ming Li Chunbo Lou bian Wu CAS Key Laboratory of Microbial Physiological&Metabolic Engineering and State Key Laboratory of Microbial Resources Institute of MicrobiologyChinese Academy of SciencesBeijing100101China College of Life Sciences University of Chinese Academy of SciencesBeijing100149China Tsinghua University Beijing100084China

The development of base editing(BE)technology has opened a new avenue for research studies in bacteriology,particularly for bacterial species in which the DNA double-strand breaks(DSBs)introduced by CRISPR/Cas system would lead to cell ***,a major limitation of BE-mediated gene editing is the restricted editable sites in the target bacterial genome due to highly diverse genomic compositions,such as GC ***,we developed a broad-spectrum DNase-inactive Cpf1(dCpf1)variant from Francisella novicida(bsdFnCpf1)through directed *** resulting optimized mutant showed a substantially expanded targeting range,including previously non-canonical protospacer-adjacent motifs(PAMs),especially the GC-rich *** deaminase APOBEC1 and uracil DNA glycosylase inhibitor(UGI)were fused with bsdFnCpf1 to achieve specific C to T mutations at multiple target sites with canonical or non-canonical PAMs in the *** genome without compromising cell *** anticipate that bsdFnCpf1 could be applied for multiplex gene regulation and BE in species that have been reported to be suitable for Cpf1.

关键词： Base editing Directed evolution Broad-spectrum dFnCpf1(bsdFnCpf1) Multiple gene editing

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Preparation and lithium storage performances of g-C_(3)N_(4)/Si nanocomposites as anode materials for lithium-ion battery

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Frontiers in Energy 2020年第4期14卷 759-766页

作者： Zhengxu bian zehua TANG Jinfeng XIE Junhao ZHANG Xingmei GUO Yuanjun LIU Aihua YUAN Feng ZHANG Qinghong KONG School of Environmental and Chemical Engineering Jiangsu University of Science and TechnologyZhenjiang 212003China Marine Equipment and Technology Institute Jiangsu University of Science and TechnologyZhenjiang 212003China Key Laboratory for Advanced Technology in Environmental Protection of Jiangsu Province Yancheng Institute of TechnologyYancheng 224051China School of the Environment and Safety Engineering Jiangsu UniversityZhenjiang 212013China

As the anode material of lithium-ion battery,silicon-based materials have a high theoretical capacity,but their volume changes greatly in the charging and discharging *** ameliorate the volume expansion issue of silicobased anode materials,g-C_(3)N_(4)/Si nanocomposites are prepared by using the magnesium thermal reduction *** is well known that g-C_(3)N_(4)/Si nanocomposites can not only improve the electronic transmission ability,but also ameliorate the physical properties of the material for adapting the stress and strain caused by the volume expansion of silicon in the lithiation and delithiation *** g-C_(3)N_(4)/Si electrode is evaluated,the initial discharge capacity of g-C_(3)N_(4)/Si nanocomposites is as high as 1033.3 mAh/g at 0.1 A/g,and its reversible capacity is maintained at 548 mAh/g after 400 ***,the improved rate capability is achieved with a relatively high reversible specific capacity of 218 mAh/g at 2.0 A/*** superior lithium storage performances benefit from the unique g-C_(3)N_(4)/Si nanostructure,which improves electroconductivity,reduces volume expansion,and accelerates lithiumion transmission compared to pure silicon.

关键词： magnesium thermal reduction g-C_(3)N_(4)/Si nanocomposites volume expansion electroconductivity lithium-ion battery

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基于利益相关者视角的矿山企业社会责任研究

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生态经济 2014年第8期30卷 111-117页

作者：卞娜苗泽华石家庄经济学院商学院河北石家庄050031

企业社会责任是现代公司理论的重要组成部分,日益受到理论界与学术界的关注。作为我国的基础产业,矿山企业在国民经济与社会发展中具有重要的地位和作用,在追求利润的同时也应在地方和国家发展中承担相应的社会责任。文章以利益相关者... 详细信息

企业社会责任是现代公司理论的重要组成部分,日益受到理论界与学术界的关注。作为我国的基础产业,矿山企业在国民经济与社会发展中具有重要的地位和作用,在追求利润的同时也应在地方和国家发展中承担相应的社会责任。文章以利益相关者理论为基础,从生态、经济及社会角度对我国矿山企业社会责任承担进行了研究,并提出一定的建议。

关键词：利益相关者矿山企业社会责任

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微观视角下的企业生态责任国外研究述评

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生态经济 2013年第8期29卷 105-110页

作者：卞娜苗泽华石家庄经济学院商学院河北石家庄050031

企业生态责任日益受到国内外学者的关注。文章从微观的角度出发,基于国外的相关研究成果,对企业生态责任的演进、伦理层面的研究与发展、对企业的影响研究及企业生态责任履行的方式进行了分析和述评,并对相关研究成果及其启示进行了总结。

关键词：企业生态责任伦理绩效竞争优势

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喷油润滑聚醚醚酮的接触疲劳性能

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中国机械工程 2024年第2期35卷 221-228页

作者：吴若魏沛堂谢怀杰边疆卢泽华刘怀举重庆大学机械传动国家重点实验室重庆400044 吉林中研高分子材料股份有限公司长春130113

聚醚醚酮(PEEK)是一种高强度、耐热的工程塑料,但其接触疲劳基础数据的缺失制约了它在重载场合下的高可靠、长寿命服役。开展了喷油润滑下的PEEK滚子滚动接触疲劳试验与PEEK齿轮接触疲劳试验,绘制了接触疲劳S-N曲线。对比发现,PEEK滚子... 详细信息

聚醚醚酮(PEEK)是一种高强度、耐热的工程塑料,但其接触疲劳基础数据的缺失制约了它在重载场合下的高可靠、长寿命服役。开展了喷油润滑下的PEEK滚子滚动接触疲劳试验与PEEK齿轮接触疲劳试验,绘制了接触疲劳S-N曲线。对比发现,PEEK滚子滚动接触疲劳极限比齿轮接触疲劳极限高14%,接触压力135 MPa下的滚子滚动接触疲劳寿命比齿轮接触疲劳寿命长58%,进而提出PEEK的滚子-齿轮接触疲劳寿命转换公式。

关键词：聚醚醚酮齿轮滚子接触疲劳应力-寿命曲线

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