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Metabolomics mechanism of traditional soy sauce associated with fermentation time

Food Science and Human Wellness 2022年第2期11卷 297-304页

作者： Li Zhu Siyu He Ying Lu Jianhong Gan Ningping Tao Xichang Wang zaoli jiang Yuanxiang Hong Changhua Xu College of Food Science and Technology Shanghai Ocean UniversityShanghai 201306China Shanghai Engineering Research Center of Aquatic-Product Processing&Preservation Shanghai 201306China Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation(Shanghai) Ministry of AgricultureShanghai 201306China Department of Pharmacology Yale UniversityNew HavenCT06520US Xiamen Gulong Food Co. Ltd.Xiamen 361000China National R&D Branch Center for Freshwater Aquatic Products Processing Technology(Shanghai) Shanghai 201306China

Given that fermentation time has significant impact on quality and flavor of soy sauce,a global understanding of the metabolic mechanism as fermentation time prolonged is essential for producing satisfactory and consistent quality of traditional soy sauce.Herein,the metabolic compounds changes of soy sauce associated with fermentation time up to 10 years were comprehensively investigated by using Chinese traditional fermented soy sauce(CTFSS)as a demonstration.Results showed that formaldehyde nitrogen,total soluble nitrogen(TSN),non-salt soluble solids,amino acids,free 5’-nucleotides and volatile compounds in CTFSS changed obviously during fermentation.Specifically,glutamic acid and aspartic acid were prominent in CTFSS.Continuous decrease in content of hypoxanthine(Hx)was found from 1 M(1-month soy sauce)to7 M(7-months soy sauce).Furthermore,a significant opposite tendency for changes between some volatile compounds and amino acids was indicated that there was a transformation between these two components.Therefore,a better understanding to the influence of fermentation time on soy sauce had been proposed.As the formation and conversion of amino acids and sugars might be mainly responsible for flavor formation in CTFSS,the ratio of these two reactions rate led the metabolism to be divided into three steps,degradation,conversion and balance.

关键词： Soy sauce Fermentation Amino acids Volatile compounds Flavor Metabolomics

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Chemosynthesis Pathway and In Vitro Bioactivities of Saponins in Radix and Flower of Panax Notoginseng

Chemosynthesis Pathway and In Vitro Bioactivities of Saponin...

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第十四届全国中药和天然药物学术研讨会

作者： Xin Zhou Linlin Chen Wing Lam Zijia Zhang zaoli jiang Ruifang Xie Yungchi Cheng Yale University School of Medicine

Panax notoginseng is a well known Chinese medicinal plant.Its radix is used in the history while its flower is recently used for health care.In this study we compared chemical ingredients and bioactivities in cell culture for radix and flower of panax notoginseng.The liquid chromatography-mass spectrometry system was applied to determine the contents of saponins in flower and radix of panax notoginseng.Transcription specific luciferase reporter assay and qPCR method for selected RNA were carried out to assess the impacts of flower and radix extract on the transcription signal pathways.The results of chemical analysis showed that the contents of saponins in flower and radix are very different:the contents of Rg1,Rb1,Re,R1,Rg3-20 R,Rh1 and Rf in radix are abundant;in contrast,the contents of Rb3,Fc,Ft1,Rb2 and Rh2-20 s in flowers are plentiful.There are substantial variations of those saponin contents from one source vs another.Based on relative content of saponins,the chemosynthesis pathway of ingredients in radix and flower are proposed:for radix,both PPT(Protopanaxatriol) and PPD(Protopanaxadiol) type triterpenoids are involved,the main pathway is PPT→Rb →Rg→R or PPD→Rh 20s→Rg3(20s) →R→Rb;for flowers,only PPD is main passage with PPD →Rh(20s)→Rg(20s) →Rd→Rb→Fc.The results of signal transcription assays demonstrated that herb water extract of radix and flower had no significant influences on most of transcription activities.However,total saponins of radix and flower which have highly content of saponins were able to inhibit the inflammatory related transcriptional activities and their related mRNA expression of IFNα,TNFα,il-6 and TGFβ as well as induce anti-oxygen NrF2 activities.In summary,although chemical ingredients and chemosynthesis pathway of flower and radix for panax notoginseng were different,these differences might result in their differences of pharmacological activities although our transcription pathway analysis in cell culture study may not reflect the biological activities.

关键词： flower of Panax notoginseng radix of panaxnotogiseng chemosynthesis pathway saponins luciferase assay LC-MS

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The Number of Intestinal Bacteria Is Not Critical For the Enhancement of the Antitumor Activity and Reduction of Intestinal Toxicity of Irinotecan by Chinese Medicinal Formula PHY906（KD018）

The Number of Intestinal Bacteria Is Not Critical For the En...

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第十四届全国中药和天然药物学术研讨会

作者： Wing Lam zaoli jiang Fulan Guan Rong Hu Shwu-Huey Liu Edward Chu Yung-Chi Cheng Yale University School of Medicine University of Pittsburgh Cancer InstitutePittsburgh

＜正＞The four-herb Chinese Medicine PHY906（KD108）could enhance irinotecan’s antitumor activity on colon cancer and promote the recovery of irinotecan-damaged intestines.Here,we access whether intestinal bacteria play critical role for PHY906 to achieve the above activities in murine colon-38 tumor bearing mice.Intestinal bacteria was depleted using

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The Effects of Chinese Herbal Medicine for Miscarriage on Decidual Micro-environment

The Effects of Chinese Herbal Medicine for Miscarriage on De...

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第十四届全国中药和天然药物学术研讨会

作者： Longzhu Piao Chie-Pein Chen Yung-Chi Cheng Rachel Masch Frederick Schatz Charles J.Lockwood Shwu-Huey Liu jiang zaoli jiang S.Joseph Huang The Ohio State University

Objective Spontaneous abortion(SA)complicates about 15%of clinically recognized pregnancies and displays a pro-inflammatory decidual environment.To date,there is no effective prevention for SA.JLFC01 is a multi-herbal...

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肛门内括约肌侧方半切结扎塑型术治疗陈旧性肛裂的临床观察

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上海中医药杂志 2018年第6期52卷 56-58页

作者：蒋伟冬殷立新申早立陆金根王佳雯上海中医药大学附属龙华医院肛肠科上海200032

目的观察肛门内括约肌侧方半切结扎塑型术治疗陈旧性肛裂的疗效。方法将60例陈旧性肛裂患者随机分为治疗组和对照组,每组30例。对照组采用肛门内括约肌后切开术治疗,治疗组采用肛门内括约肌侧方半切结扎塑型术治疗。观察比较两组患者术... 详细信息

目的观察肛门内括约肌侧方半切结扎塑型术治疗陈旧性肛裂的疗效。方法将60例陈旧性肛裂患者随机分为治疗组和对照组,每组30例。对照组采用肛门内括约肌后切开术治疗,治疗组采用肛门内括约肌侧方半切结扎塑型术治疗。观察比较两组患者术后疼痛、术后出血、创面面积、创面愈合时间等情况。结果 (1)组间比较,治疗组术后第1天、第3天、第7天疼痛、出血积分均低于同期对照组(P<0.05)。(2)组间比较,治疗组术后第1天、第3天、第7天、第14天创面面积评分均低于同期对照组(P<0.05)。(3)两组创面愈合时间比较,差异有统计学意义(P<0.05)。结论肛门内括约肌侧方半切结扎塑型术治疗陈旧性肛裂,能减少术后出血、减轻术后疼痛、缩小创面面积并缩短创面愈合时间。

关键词：肛裂内括约肌挂线结扎

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维普期刊数据库

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中果早栗不同采收期主要营养成分测定

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湖北农业科学 2012年第22期51卷 5176-5179页

作者：程华李琳玲姜德志许锋王燕程水源经济林木种质改良与资源综合利用湖北省重点实验室/黄冈师范学院化学与生命科学学院湖北黄冈436100 湖北省林业厅科技教育处武汉430079

以罗田板栗主栽品种中果早栗为试材,研究其不同采收期主要营养成分含量的变化。结果表明,随着板栗的成熟,其水分逐渐降低,而淀粉、蛋白质、可溶性糖、脂肪和维生素C都呈上升趋势,成熟Ⅰ期和成熟Ⅱ期的水分、可溶性糖、脂肪和维生素C含... 详细信息

以罗田板栗主栽品种中果早栗为试材,研究其不同采收期主要营养成分含量的变化。结果表明,随着板栗的成熟,其水分逐渐降低,而淀粉、蛋白质、可溶性糖、脂肪和维生素C都呈上升趋势,成熟Ⅰ期和成熟Ⅱ期的水分、可溶性糖、脂肪和维生素C含量差异不显著,淀粉和蛋白质含量差异显著;而未成熟中果早栗与成熟Ⅰ期和成熟Ⅱ期的中果早栗各营养成分均具有显著差异。充分成熟的板栗,果实代谢处于最低水平,具有较好的商业贮藏性能。为防止板栗充分成熟后栗苞开裂致使栗果落地造成损失,建议在成熟期Ⅰ时一次性打落进行采收。

关键词：中果早栗采收期成熟度主要营养成分

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维普期刊数据库

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‘早金酥’梨授粉品种选择及果实品质分析

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北方果树 2018年第6期 10-12页

作者：姜晓艳李俊才王家珍沙守峰蔡忠民李宏军辽宁省果树科学研究所辽宁熊岳115009

为筛选适合‘早金酥’梨的授粉品种,选用10个梨品种为‘早金酥’梨授粉,测定坐果率、单果重、果实硬度、总糖含量等指标。结果表明,除‘蔗梨’外,其余9个授粉品种授粉的坐果率均在62.5%以上;不同授粉品种对‘早金酥’梨果实的外观和内... 详细信息

为筛选适合‘早金酥’梨的授粉品种,选用10个梨品种为‘早金酥’梨授粉,测定坐果率、单果重、果实硬度、总糖含量等指标。结果表明,除‘蔗梨’外,其余9个授粉品种授粉的坐果率均在62.5%以上;不同授粉品种对‘早金酥’梨果实的外观和内在品质均有影响,花粉直感效应明显。综合坐果率、单果重、产量、果实硬度、总糖含量等各项指标,以及授粉品种在当地的适应性,认为‘金酥’‘早酥’‘华酥’‘早梨18’为适宜‘早金酥’梨的授粉品种。

关键词： '早金酥’梨授粉品种果实品质

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