检索结果-南通市图书馆

Case Reports in Clinical Medicine 2017年第10期6卷 274-280页

作者： Pierre-Antoine Pradervand Roland Antaki Simon Phillips C. Sylvestre zaki el haffaf Ariane Godbout Isaac Jacques Kadoch Pierre Lehmann OVO Fertility OVO Clinic Montreal Canada Assisted Procreation Clinic Centre Hospitalier de l’Université de Montréal (CHUM) Montreal Canada Division of Reproductive Endocrinology and Infertility Centre Hospitalier Universitaire (CHU) Ste-Justine Montreal Canada Department of Obstetrics and Gynecology H&ocircpital Sacré Coeur de Montréal Montreal Canada

A 19 years old patient with primary amenorrhea was referred to our center. Based on discrepancy between high follicle stimulating hormone (FSH) level and normal ovarian reserve parameters, follicle stimulating hormone receptor (FSHR) mutation was screened. The patient was homozygous in exon 6 of the FSHR gene for the new variant c.479T > C and predicted to result in an aminoacid substitution ***160Thr. One year later, her anti-müllerian hormone (AMH) level inexplicably decreased. Oocyte vitrification was thus offered for fertility preservation. After 17 days of recombinant follicle stimulating hormone (recFSH) (900 IU daily), no follicular growth was seen and estradiol levels remained low. In vitro maturation (IVM) was then suggested. Ten oocytes were successfully vitrified.

关键词： FSH Receptor Mutation Fertility Preservation in Vitro Maturation AMH Primary Ovarian Insufficiency

Study of enteropathogens associated with paediatric gastroenteritis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2009年第3期2卷 63-69页

作者： Maysaa el Sayed zaki Clinical Pathology Faculty of MedicineMansoura UniversityEgyt

Objective:To determine the etiology of acute diarrhea in children under 5 years of age and to improve- knowledge of the etiology of gastrointestinal pathogens using traditional and molecular diagnostic ***: Various common enteropathogens(viral,bacterial and parasites) associated with diarrhea were investigated by conventional and molecular techniques(PCR) for verotoxin present in Escherichia coli in 218 children less than 5 years of age admitted to Mansoura University Children hospital- ***:The occurrence of enteropathogens identified was as follows:*** O157:H7 38.8%followed by Salmonella Spp 29.4%,Aeromonas 20%and Shigella Spp 11.8%.Rotavirus was found in of samples 17.1%.Rotavirus was statistically significant in ageella isolates were tested for their susceptibility to common antimicrobial agents and most of the isolates were resistant to ampicillin and trimethoprim /***:This study demonstrated that rotavirus,*** O157:H7,Salmonella Spp,and Aeromonas were significant *** was significantly associated with infantile *** results highlight the value of using a combination of traditional and PCR techniques in the diagnosis of enteropathogens related to acute gastroenteritis in children.

关键词： Acute diarrhea Children Parasites Bacteria

Evaluation of molecular and immunological methods for diagnosis of tuberculosis pleurisy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2008年第4期1卷 14-19页

作者： Maysaa el Sayed zaki Clinical Pathology Faculty of MedicineMansoura UniversityEgypt

Objective:The inefficiency of conventional laboratory methods for diagnosis of Pleural tuberculosis(TBP) and the reliance on pleural biopsy have motivated the evaluation of alternative diagnostic *** goal was to evaluate different laboratory techniques Ziehl Neelsen,Mantoux skin test,determination of interferon gamma in serum and pleural fluid,polymerase chain reaction and serological study of specific IgG,IgM and IgA beside bacteriological culture by BACTEC 460 TB for rapid and accurate diagnosis of tuberculosis pleurisy. Methods:Patients presented with pleural effusions were subjected to study by ZN,PCR,serological study By specific IgG,IgM and IgA for A60 of tuberculosis compared to culture by BACTEC460 *** gamma was determined both in serum and pleural ***:Mantoux skin test was positive in 19 TBP patients and four patients with exudative pleural effusion.Z.N staining results of pleural biopsy specimens were positive in only 1 of 23 patients(4.3%) in the tuberculous pleural effusion *** was positive in 20 cases of group 1(87%).Serum and pleural fluid interferon had significantly elevated levels(Pelation in TBP(PelISA test was positive in 7/23(30.4%),IgM was positive in 17/23(73.9%) patients and IgG was positive in 16/23(69%) *** of the non TBP had either ZN,PCR or positive serum IgA,IgG,or *** the positive results for IgG and IgM were combined together the serological tests correcdy identified 20/23(87%) of patients. Conclusion:We suggest that in TBP serological diagnosis by combined use of IgG and IgM for A60 antigen with serum determination of interferon gamma can provide rapid and non invasive diagnostic tool that can justify the starting of chemotherapy while awaiting the results of culture.

关键词： Tuberculous pleurisy Molecular diagnosis Tuberculin Serology Interferon gamma

Molecular study of astrovirus, adenovirus and norovirus in community acquired diarrhea in children: One Egyptian center study

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2017年第11期7卷 987-990页

作者： Maysaa el Sayed zaki Nermen Abo el Kheir Clinical Pathology Department Mansoura Faculty of MedicineMansoura UniversityEgypta

Objective: To determine the prevalence of astrovirus, norovirus, adenovirus in children below five years old with diarrhea by multiplex reverse transcriptase polymerase chain reaction(RT-PCR) along with rotavirus antigen detection by enzyme linked immunosorbant ***: The study was conducted on children below five years old complaining of acute diarrhea. The study included stool examination by molecular method for detection of norovirus, adenovirus and astrovirus by multiplex RT-PCR. Rotavirus antigen was detected in the stool by enzyme linked immunosorbant ***: The study included 100 children below 5 years old with acute *** RT-PCR was positive in 34% of the children. The most frequently detected virus was rotavirus(44%), followed by norovirus(30%), adenovirus(20%) and astrovirus(14%). The clinical symptoms were more significantly associated with viral diarrhea such as fever(P = 0.03), bloody diarrhea(P = 0.025), vomiting(P = 0.000 1) and watery diarrheas(P = 0.05). The frequency of diarrhea with viral pathogen was significantly presented in winter season(39.7%). There were significant frequencies of norovirus and adenovirus in age ranging 1–2 years old(P = 0.04, P = 0.01 respectively).Conclusions: The present study spotlights on the prevalence of viral pathogens as an important etiology in diarrhea in children below five years old. Astrovirus, norovirus and adenovirus are common along with rotavirus in this group of patients. Multiplex PCR leads to improve the laboratory diagnosis of these viruses along with antigen detection method. Further longitudinal studies are required to evaluate the epidemiological data associated with these viruses and for proper management of such drastic infection.

关键词： Diarrhea Astrovirus Adenovirus Norovirus Multiplex-RT PCR

Correlation of biofilm formation of uropathogenic Escherichia coli(UPEC) and fimbriae genotypes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2009年第5期2卷 49-53页

作者： Maysaa el Sayed zaki Clinical Pathology Department Faculty of Medicine-Mansoura UniversityEgypt

Objective:In the present study we try to correlate between pathogenic intrinsic factor of Escherichia coli(***) presented with different fimbria genotyes and biofilm formation with host immune factor entitled interleukin-6(IL-6) secretion as defense ***:A total of 91 pediatrics complaining of pyuria were included in the present *** addition,20 healthy control children were *** microbiological study was performed for isolated *** alleles were studied by multiple alleles PCR and biofilm formation was ***-6 was measured in ***:IL-6 had statistically significant elevation in patients’urine compared to *** biofilm study, it was found that 19 isolated *** had formed biofilm in ***,urine samples with positive biofilm formation of *** had statistically significant lower IL-6 secretion than those with negative *** for *** distribution of fimbria genotypes showed that the frequent genotype was for alleleⅠ(34.3%) followed by mixed allelesⅠandⅡ(24.1%).There was significant correlation between mixed alleles(Ⅰ&Ⅱ)and biofilm ***: The present study highlights the presence of significant strains of *** causing urinary tract infections capable of biofilm *** formation is associated with less innate immunity manifested by lower urinary IL- *** majority of isolates had fimbria *** appears that mixed allelesⅠandⅡhave prominent virulence effect with tendency for biofilm formation.

关键词： Uropathogenic Escherichia coli Interleukin -6(IL-6) Urinary tract infection Biofilm

Value of avidity index of IgG and polymerase chain reaction in diagnosis of herpes simplex 2 virus in pregnancy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2009年第2期2卷 30-34页

作者： Maysaa el Sayed zaki Clinical Pathology Faculty of MedicineMansoura UniversityEgypt

Objective:The goal of the present study is to highlight the significance of the determination of IgG avidity to herpes simplex2virus(HSV2) compared to detection of viremia by polymerase chain reaction(PCR) in relation to recurrent ***:Serum samples from pregnant patients with bad obstetric histories and control subjects were analyzed for quantitative determination of IgG specific for HSV2 by using elISA with and without sample treatment with 8 M urea as protein-denaturing agents for measurement of avidity index(AI).PCR was performed for samples to determine HSV2 ***:Regarding avidity index 11.5%had avidity index = 100.0,11.5%has avidity index = 47%and 76.9%had avidity index>60%.Control group had AI>70%.There was statistically insignificant difference between OD of IgG in patients with viremia before urea treatment and after urea treatment compared to healthy *** there was statistically insignificant difference in OD before urea and after urea in patients with ***:From this study we can conclude that viremia for HSV2 is common finding in patients with bad obstetric *** avidity immunoglobulin G was not common finding in those *** may be explain that even reactivation of latent HSV 2 infection is associated with fetal loss.

关键词： Herpes simplex 2 PCR IgG avidity test

Course Evaluation Based on Deep Learning and SSA Hyperparameters Optimization

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Computers, Materials & Continua 2022年第4期71卷 941-959页

作者： Alaa A.el-Demerdash Sherif E.Hussein John FW zaki Department of Computer and Systems Faculty of EngineeringMansoura UniversityMansoura35516Egypt

Sentiment analysis attracts the attention of Egyptian Decisionmakers in the education *** offers a viable method to assess education quality services based on the students’feedback as well as that provides an understanding of their *** machine learning techniques offer automated strategies to process big data derived from social media and other digital channels,this research uses a dataset for tweets’sentiments to assess a few machine learning *** dataset preprocessing to remove symbols,necessary stemming and lemmatization is performed for features *** is followed by several machine learning techniques and a proposed Long Short-Term Memory(LSTM)classifier optimized by the Salp Swarm Algorithm(SSA)and measured the corresponding ***,the validity and accuracy of commonly used classifiers,such as Support Vector Machine,Logistic Regression Classifier,and Naive Bayes classifier,were ***,LSTM based on the SSA classification model was compared with Support Vector Machine(SVM),Logistic Regression(LR),and Naive Bayes(NB).Finally,as LSTM based SSA achieved the highest accuracy,it was applied to predict the sentiments of students’feedback and evaluate their association with the course outcome evaluations for education quality purposes.

关键词： Sentiment analysis course evaluation deep learning Bi-LSTM opinion mining students feedback natural language processing machine learning tweets analysis SSA

Dietary effects of A zolla pinnata combined with exogenous digestive enzyme(Digestin^(TM)) on growth and nutrients utilization of freshwater prawn, Macrobrachium rosenbergii(de Man 1879)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Oceanology and Limnology 2018年第4期36卷 1434-1441页

作者： GODA Ashraf SAAD Amal HANAFY Mohamed SHARAWY zaki el-HAROUN Ehab Aquaculture Division National Institute of Oceanography and Fisheries (NIOF) Institute of Oceanology Chinese Academy of Sciences Division of Aquaculture College of Agriculture Food Science and Sustainable Systems Kentucky State University

The present study was conducted to evaluate the effect of either individual or combined wheat bran(WB) replacement with Azolla pinnata supplemented with Digestin^(TM) in the diet of freshwater prawn, Macrobrachium rosenbergii Postlarvae(PL) on growth performance, nutrient utilization, chemical body composition and survival(%). Experimental diets were a wheat bran-soybean based diet with no Azolla and Digestin^(TM)(control, T_1), and diets containing 17% Azolla supplemented with Digestin^(TM) 0%(T_2), 1%(T_3), 2%(T_4) and 3%(T 5). Each experimental diet was allocated into three tanks(6 m^3/tank) fed for 12 wks. Each tank was subdivided into three equal pens by nets(2 m^3) and stoked with 84 PL/m^2. The experimental diets were readily consumed by prawns PLs where both high growth and good feed efficiency were achieved for all diets. The results showed that the diets containing A. pinnata supplemented with Digestin? at the level up to 3% have the higher growth and better nutrient utilization than the control diet. No differences were observed for moisture and protein content among the experimental diets. However, the highest protein content was observed on prawns fed on diets T_1 and T 5 respectively, while the lowest value was recorded for T_4 diet. The results also show that prawn PLs fed the diets contain A. pinnata and supplemented with Digestin^(TM) recorded the highest values of body lipid content compared to the control diet. Feed efficiency and economic conversion rate(ECR) values show that economic performance and the cost-effectiveness of the A. pinnata supplemented with up to 3% Digestin^(TM) recorded the highest net return, and therefore it is recommended for prawn, M. rosenbergii PL's. These results are clearly indicating that A. pinnata have a good potential for use in prawn diets at reasonable levels than other conventional diets.

关键词： Azolla meal enzymes growth performance economic analysis feed efficiency

Dipper Throated Optimization Algorithm for Unconstrained Function and Feature Selection

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Computers, Materials & Continua 2022年第7期72卷 1465-1481页

作者： Ali E.Takieldeen el-Sayed M.el-kenawy Mohammed Hadwan Rokaia M.zaki Faculty of Artificial Intelligence Delta University for Science and TechnologyMansoura35712Egypt Department of Communications and electronics Delta Higher Institute of Engineering and TechnologyMansoura35111Egypt Department of Information Technology College of ComputerQassim UniversityBuraydah51452Saudi Arabia Department of Computer Science College of Applied SciencesTaiz UniversityTaizYemen Intelligent Analytics Group(IAG) College of ComputerQassim UniversityBuraydahSaudi Arabia Higher Institute of Engineering and Technology KafrelsheikhEgypt Department of electrical Engineering Shoubra Faculty of EngineeringBenha UniversityEgypt

Dipper throated optimization(DTO)algorithm is a novel with a very efficient metaheuristic inspired by the dipper throated *** has its unique hunting technique by performing rapid bowing *** show the efficiency of the proposed algorithm,DTO is tested and compared to the algorithms of Particle Swarm Optimization(PSO),Whale Optimization Algorithm(WOA),Grey Wolf Optimizer(GWO),and Genetic Algorithm(GA)based on the seven unimodal benchmark ***,ANOVA and Wilcoxon rank-sum tests are performed to confirm the effectiveness of the DTO compared to other optimization ***,to demonstrate the proposed algorithm’s suitability for solving complex realworld issues,DTO is used to solve the feature selection *** strategy of using DTOs as feature selection is evaluated using commonly used data sets from the University of California at Irvine(UCI)*** findings indicate that the DTO outperforms all other algorithms in addressing feature selection issues,demonstrating the proposed algorithm’s capabilities to solve complex real-world situations.

关键词： Metaheuristic optimization swarmoptimization feature selection function optimization

Evaluation of DNA damage profile in obese women and its association to risk of metabolic syndrome,polycystic ovary syndrome and recurrent preeclampsia

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2018年第4期5卷 367-373页

作者： Moushira zaki Walaa Basha Hala T.el-Bassyouni Safinaz el-Toukhy Tamer Hussein Biological Anthropology Department Medical Research DivisionNational Research CentreCairoEgypt Clinical Genetics Department National Research CentreCairoEgypt Medical Biochemistry Department Medical Research DivisionNational Research CentreCairoEgypt Reproductive Health Research Department Medical Research DivisionNational Research CentreCairoEgypt

Metabolic syndrome(MS)is a cluster of metabolic *** and MS are always accompanied by elevated oxidative stress which might affect cellular biomolecules such as *** aim of the present study is to investigate DNA damage profile in obese premenopausal women and its relation to the risk of MS,polycystic ovary syndrome(PCOS)and history of recurrent *** study included 90 obese women included cases with MS(nZ30),PCOS(n Z30)and previous history of recurrent preeclampsia(nZ30)and,age-matched healthy non-obese control women(n Z 50).The assessment of leukocyte DNA damage was done by comet assay for all cases and *** and biochemical parameters have been *** showed that mean percent of DNA damage was significantly higher in MS,PCOS as well as in women with the recurrent preeclampsia as compared to healthy *** high level of mean DNA damage frequency in obese women was significantly associated with the increased number of metabolic syndrome *** with 2,3 and 3e5 components showed significantly higher levels of DNA damage than ***,cases with 3e5 MS components showed significant higher DNA compared to those with the two *** PCOS,significant positive association between the mean frequency of DNA damage and waist circumference was *** study suggests that metabolic abnormalities,PCOS and recurrent pre-eclampsia might be contributed in development of DNA damage in obese *** damage can serve as an early marker for obesity complications in premenopausal women.

关键词： DNA damage Metabolic components Obesity PCOS Recurrent preeclampsia