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Evaluation of Disk Potentiation Test (DPT) and Double Disk Synergy Test (DDST) for The Detection of Metallo-β-Lactamases (MBLs) in Clinical Isolates of Bangladesh

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Advances in Infectious Diseases 2023年第4期13卷 609-626页

作者： Sumon Kumar Das Afzal Sheikh nikhat Ara Suma Mita Biswas Abhinandan Chowdhury Fatimah Az zahra Chaman Ara Keya Department of Biochemistry & Microbiology North South University Dhaka Bangladesh Department of Microbiology & Immunology Evercare Hospital Dhaka Bangladesh Department of Biochemistry and Molecular Biology Bangabandhu Sheikh Mujibur Rahman Agricultural University Dhaka Bangladesh Institute of Food Safety and Processing (IFSP) Bangabandhu Sheikh Mujibur Rahman Agricultural University Dhaka Bangladesh

Objective: Increasing the emergence of Metallo-β-lactamase (MBL) producing gram-negative bacteria and their dexterous horizontal transmission demands rapid and accurate detection. This study was conducted to determine a suitable method to promptly detect MBL-producing gram-negative bacteria. Methods: A total of 103 gram-negative bacteria were identified from various clinical samples at a tertiary care hospital in Dhaka city. MBL producers were detected by two phenotypic methods, the Disk Potentiation Test (DPT) and the Double Disk Synergy Test (DDST) based on β-lactam chelator combinations where EDTA/SMA has been used as an inhibitor and Imipenem, Ceftazidime as substrates. Results: 103 isolates which were identified as Escherichia coli spp, Klebsiella spp, Pseudomonas spp, Acinetobacter spp, Proteus spp, Providencia spp were found to be multidrug-resistant in antibiogram test. Isolates showed complete resistance (100%) to Imipenem, Meropenem, and Amoxiclav. The highest carbapenem-resistant etiological agents were Acinetobacter spp 40 (38.8%) followed by Pseudomonas spp 27 (26.2%), Klebsiella spp 26 (25.2%), Escherichia coli 8 (7.8%), Proteus spp 1 (1%) and Providencia spp 1 (1%). DPT method detected significantly (p = 0.000009) a higher number of MBL-producers (Imipenem with 0.5 M EDTA n = 61, 59.2% & Ceftazidime with 0.5 M EDTA n = 56, 54.4%) compared to the DDST method (Imipenem -0.5 M EDTA n = 43, 41.7%, Imipenem – SMA n = 38, 36.9% & Ceftazidime -0.5 M EDTA n = 15, 14.6%). Conclusion: Pieces of evidence suggest that DPT is a more sensitive method than DDST and could be recommended for identifying MBL-producing bacteria in Bangladeshi hospitals for the proper management of patients, to reduce time constraints and treatment costs.

关键词： Disk Potentiation Test (DPT) Double Disk Synergy Test (DDST) Metallo-β-Lactamase (MBL) Sodium Mercaptoacetate (SMA) and Ethylenediaminetetraacetic Acid (EDTA)

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Mapping and Characterization of Agricultural Systems from Time Series of normalized Difference Vegetation Index (nDVI) in the northeast Area of Tadla, Morocco

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natural Resources 2017年第1期8卷 24-30页

作者： Didi Salahddine Fatima Ezzahra Housni Abdessamad najine Amina Wafik Mohamed Aadraoui Fatima zahra Hafiane Humberto Bracamontes del Toro Valuation Laboratory and natural Resources Management Faculty of Science and Technology University Sultan Moulay Slimane Beni Mellal Morocco Southern Region University Center University of Guadalajara Ciudad Guzm&aacute n M&eacute xico Dynamic Laboratory of the Lithosphere and the Genesis of Mineral and Energy Resources Department of Geology Faculty of Science Semlalia University Cadi Ayyad Marrakech Morocco Laboratory of Geosciences and Environment Faculty of Science and Technology University Sultan Moulay Slimane Beni Mellal Morocco Unit Pollution Ecotoxicology and Risk Sanitary Scientific Institute Mohammed V University Rabat Morocco Cd. Guzmá n Institute of Technology Ciudad Guzm&aacute n M&eacute xico

Introduction: Agriculture is the focus of sustainable development and food security in Morocco, contributing 14% to the national economy and its socio-economic impact depends on agro-climatic conditions and the availability of water resources. The objective of this work is to demonstrate that agriculture in Morocco, and especially in the Tadla region, is distributed according to the availability of underground water. Method: This work was carried out in the transition zone between the phosphate plateau and the plain of Tadla, a site of more than 124,000 ha. To achieve the objectives set, we used Landsat-8 OLI (Operational Land Imager) data sharpened to 15 m. The Support Vector Machine (SVM) classifications were applied to the 10-period normalized Difference Vegetation Index (nDVI) series. Results: It is shown that agriculture in Morocco is highly dependent on irrigation. In the classified map, it can be observed that the southern region, a zone with a semi-arid climate, which generally relies on irrigation, is very developed in agriculture, whereas in the north most of the soil is bared with cereals that mainly depend on rainfall. Conclusion: The possible water shortage in the region puts food availability in Morocco at risk of inadequacy. Food security depends on the availability of water, a natural resource at risk of extinction. Therefore, strategies to reduce the risk of agricultural drought must be at the forefront of Moroccan policies.

关键词： Water Availability Landsat-8 OLI Crop Mapping Plain de Tadla Morocco

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蜡状芽胞杆菌nos基因簇基因克隆及生物信息学分析

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氨基酸和生物资源 2015年第1期37卷 41-48页

作者：陈元磊李丹丹李景龙 Amina zahra 袁永泽熊丽耿辉刘德立华中师范大学生命科学学院/湖北省遗传调控与整合生物学重点实验室湖北武汉430079

为了探究革兰氏阳性菌蜡状芽胞杆菌HS-n10 nos基因簇相关基因的特点,PCR扩增获得了HS-n10 nos基因簇相关基因,其中包括nosZ全长1 914bp、nosR部分片段1 749bp、nosD部分片段946bp和nosF部分片段495bp。经BLASTn分析,HS-n10 nosZ序列和n... 详细信息

为了探究革兰氏阳性菌蜡状芽胞杆菌HS-n10 nos基因簇相关基因的特点,PCR扩增获得了HS-n10 nos基因簇相关基因,其中包括nosZ全长1 914bp、nosR部分片段1 749bp、nosD部分片段946bp和nosF部分片段495bp。经BLASTn分析,HS-n10 nosZ序列和nosD序列分别与施氏假单胞菌(Pseudomonas stutzeri)相应序列相似性最高;HS-n10 nosR序列和nosF序列分别与裂环无色杆菌(Achromobacter cycloclastes)相应序列相似性最高。通过在线数据库与分析工具分别对HSn10nos基因簇相关基因进行了生物信息学分析。

关键词： nos基因簇蜡状芽胞杆菌克隆表达生物信息学分析

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An analytical model for the cut off frequency and noise of AIGan/Gan high electron mobility transistor （HEMTs）

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材料科学与工程（中英文版） 2009年第12期3卷 22-25页

作者： Rajab Yahyazadeh zahra Hashempour Manouchehr Kalafi Mohammad Reza Aboulhasani Department of Physics Islamic Azad University of KhoyBranch Khoy 58135/175 Iran Research Institute for Applied Physics Tabriz University Tabriz 51665/163 Iran Department of Physics Tarbiyat Modarres University Tehran 14115/111 Iran

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First United Arab Emirates consensus on diagnosis and management of inflammatory bowel diseases:A 2020 Delphi consensus

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World Journal of Gastroenterology 2020年第43期26卷 6710-6769页

作者： Maryam Alkhatry Ahmad Al-Rifai Vito Annese Filippos Georgopoulos Ahmad n Jazzar Ahmed M Khassouan Zaher Koutoubi Rahul nathwani Mazen S Taha Jimmy K Limdi Gastroenterology and Endoscopy Department Ibrahim Bin Hamad Obaid Allah HospitalMinistry of Health and PreventionRas Al KhaimanUnited Arab Emirates Department of Gastroenterology Sheikh Shakbout Medical CityAbu DhabiUnited Arab Emirates Department of Gastroenterology Valiant ClinicDubaiUnited Arab Emirates Department of Gastroenterology and Endoscopy American HospitalDubaiUnited Arab Emirates Gastroenterology and Endoscopy Unit Al Zahra HospitalDubaiUnited Arab Emirates Gastroenterology Division Sheikh Khalifa Medical CityAbu DhabiUnited Arab Emirates Digestive Disease Unit Rashid HospitalDubaiUnited Arab Emirates Digestive Disease Institute Cleveland ClinicAbu DhabiUnited Arab Emirates Department of Gastroenterology Mediclinic City HospitalDubaiUnited Arab Emirates Gastroenterology and Hepatology Tawam HospitalAl AinUnited Arab Emirates Department of Gastroenterology The Pennine Acute Hospitals NHS TrustManchester Academic Health SciencesUniversity of ManchesterManchester M85RBUnited Kingdom

Ulcerative colitis and Crohn’s disease are the main entities of inflammatory bowel disease characterized by chronic remittent inflammation of the gastrointestinal *** incidence and prevalence are on the rise worldwide,and the heterogeneity between patients and within individuals over time is *** progressive advance in our understanding of the etiopathogenesis coupled with an unprecedented increase in therapeutic options have changed the management towards evidence-based interventions by clinicians with *** guideline was stimulated and supported by the Emirates Gastroenterology and Hepatology Society following a systematic review and a Delphi consensus process that provided evidence-and expert opinion-based *** up-to-date guidance is provided regarding diagnosis,evaluation of disease severity,appropriate and timely use of different investigations,choice of appropriate therapy for induction and remission phase according to disease severity,and management of main complications.

关键词： Ulcerative colitis Crohn’s disease Infliximab Adalimumab Vedolizumab Ustekinumab Tofacitinib

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Fungal diversity notes 1611-1716: taxonomic and phylogenetic contributions on fungal genera and species emphasis in south China

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Fungal Diversity 2023年第5期122卷 161-403页

作者： Indunil C.Senanayake Walter Rossi Marco Leonardi Alex Weir Mark McHugh Kunhiraman C.Rajeshkumar Rajnish K.Verma Samantha C.Karunarathna Saowaluck Tibpromma nikhil Ashtekar Sreejith K.Ashtamoorthy Sanjay Raveendran Gurmeet Kour Aishwarya Singh Saúl De la Peña-Lastra Antonio Mateos Miroslav Kolařík Vladimír Antonín HanaŠevčíková Fernando Esteve-Raventós Ellen Larsson Fermín Pancorbo Gabriel Moreno Alberto Altés Yolanda Turégano Tian-Ye Du Li Lu Qi-Rui Li Ji-Chuan Kang Sugantha Gunaseelan Kezhocuyi Kezo Malarvizhi Kaliyaperumal Jizhen Fu Milan C.Samarakoon Yusufjon Gafforov Shakhnoza Teshaboeva Pradeep C.Kunjan Arya Chamaparambath Adam Flakus Javier Etayo Pamela Rodriguez-Flakus Mikhail P.Zhurbenko nimali Ide Silva Danushka S.Tennakoon KPDeepna Latha Patinjareveettil Manimohan KnAnil Raj Mark S.Calabon Abdollah Ahmadpour Zeinab Heidarian zahra Alavi Fatemeh Alavi Youbert Ghosta Razmig Azizi Mei Luo Min-Ping Zhao nuwan D.Kularathnage Li Hua Yun-Hui Yang Chun-Fang Liao Hai-Jun Zhao Anis S.Lestari Subashini C.Jayasiri Feng-Ming Yu Lei Lei Jian-Wei Liu Omid Karimi Song-Ming Tang Ya-Ru Sun Yong Wang Ming Zeng Zin H.Htet Benedetto T.Linaldeddu Artur Alves Alan J.L.Phillips Carlo Bregant Lucio Montecchio AndréDe Kesel Vincent P.Hustad Andrew n.Miller Anna G.Fedosova Viktor Kučera Mubashar Raza Muzammil Hussain Yan-Peng Chen Vinodhini Thiyagaraja Deecksha Gomdola Achala R.Rathnayaka Asha J.Dissanayake nakarin Suwannarach Sinang Hongsanan Sajeewa S.n.Maharachchikumbura Lakmali S.Dissanayake nalin n.Wijayawardene Rungtiwa Phookamsak Saisamorn Lumyong E.B.Gareth Jones neelamanie Yapa Dhanushka n.Wanasinghe ning Xie Mingkwan Doilom Ishara S.Manawasinghe Jian-Kui(Jack)Liu Qi Zhao Biao Xu Kevin D.Hyde Jiage Song Innovative Institute for Plant Health/Key Laboratory of Green Prevention and Control On Fruits and Vegetables in South China Ministry of Agriculture and Rural AffairsZhongkai University of Agriculture and EngineeringHaizhu DistrictGuangzhou 510225GuangdongChina Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand Guangdong Provincial Key Laboratory for Plant Epigenetics College of Life Science and OceanographyShenzhen University3688Nanhai AvenueNanshanShenzhen 518055GuangdongChina Sect.Environmental Sciences Department MeSVAUniversity of L’AquilaVia Vetoio67100 CoppitoAQItaly Department of Environmental Biology SUNY-ESF241 Illick Hall1 Forestry DriveSyracuseNY 13210USA national Fungal Culture Collection of India MACS Agharkar Research InstitutePuneMaharashtra 411004India Department of Plant Pathology Punjab Agricultural UniversityLudhianaPunjab 141004India Center for Yunnan Plateau Biological Resources Protection and Utilization College of Biological Resource and Food EngineeringQujing Normal UniversityQujing 655011YunnanChina Department of Forest Ecology Kerala Forest Research InstitutePeechiThrissurKerala 680653India Department of Botany The American CollegeTallakkulamMaduraiTamil Nadu 625002India Department of Zoology Savitribai Phule Pune UniversityGaneshkhind RdGaneshkhindPuneMaharashtra 411007India University of Santiago de Compostela 15782 Santiago de CompostelaSpain Sociedad Micológica Extremeña C/Sagitario 1410001 CáceresSpain Institute of Microbiology of the Czech Academy of Sciences Vídeňská108314220 Prague 4Czech Republic Department of Botany Moravian MuseumZelnýtrh 665937 BrnoCzech Republic Department of Life Sciences(Botany Unit) University of Alcalá28805 Alcaláde HenaresMadridSpain Biological and Enviromental Sciences University of Gothemburgand Gothemburg Global Biodiversity CentreBox 46140530 GöteborgSweden Madrid Mycological Society Real Jardín BotánicoC/Claudio Moyano 128014 MadridSpain State Ke

This article is the 15th contribution in the Fungal Diversity notes series,wherein 115 taxa from three phyla,nine classes,28 orders,48 families,and 64 genera are *** taxa described and illustrated in the present study include a new family,five new genera,61 new species,five new combinations,one synonym,one new variety and 31 records on new hosts or new geographical *** *** introduced and accommodated in *** new genera introduced in this study are Ageratinicola,Kevinia,Pseudomultiseptospora(Parabambusicolaceae),Marasmiellomycena,and Vizzinia(Porotheleaceae).newly described species are Abrothallus altoandinus,Ageratinicola kunmingensis,Allocryptovalsa aceris,Allophoma yuccae,Apiospora cannae,***,***,Boeremia wisteriae,Calycina papaeana,Clypeo-coccum lichenostigmoides,Coniochaeta riskali-shoyakubovii,Cryphonectria kunmingensis,Diaporthe angustiapiculata,***,***,Diatrypella guangdongense,Dothiorella franceschinii,Endocalyx phoenicis,Epicoc-cum terminosporum,Fulvifomes karaiensis,***,Ganoderma ghatensis,Hysterobrevium baoshanense,Inocybe avellaneorosea,***,Jahnula oblonga,Kevinia lignicola,Kirschsteiniothelia guangdongensis,Laboulbenia caprina,***,***,***,***,***,***,***,***,Laccaria rubriporus,Lasiodiplodia morindae,Lyophyllum agnijum,Marasmiellomycena pseudoomphaliiformis,Melomastia beihaiensis,nemania guangdongensis,nigrograna thailandica,nigrospora ficuum,Oxydothis chinensis,***,Petriella thailandica,Phaeoacremonium chinensis,Phialocephala chinensis,Phytophthora debattistii,Polyplosphaeria nigrospora,Pronectria loweniae,Seriascoma acutispora,Setoseptoria bambusae,Stictis anomianthi,Tarzetta tibetensis,Tarzetta urceolata,Tetraploa obpyriformis,Trichoglossum beninense,and Tricoderma *** provide an emendation for Urnula ailaoshanensis Agaricus duplocingulatoides *** i

关键词： Agaricomycetes Ascomycota Basidiomycota Dothideomycetes Eurotiomycetes Fungal diversity Geoglossomycetes Geographical distribution Host records Incertae sedis Laboulbeniomycetes Lecanoromycetes Leotiomycetes novel taxa new combination Oomycota Peronosporea Pezizomycetes Phylogeny Sordariomycetes South Chinese fungi Taxonomy

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中国秃疮花属和海罂粟属的细胞学研究（英文）

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植物分类与资源学报 2015年第6期37卷 721-726页

作者：向春雷董洪进胡国雄纳迪亚.扎赫拉船本常男中国科学院东亚植物多样性与生物地理学重点实验室昆明650201 国家林业局昆明勘察设计院昆明650216 伊斯兰堡真纳大学生物技术系巴基斯坦伊斯兰堡45320 昭和药科大学药学基础教育与研究中心生物研究所

报道了秃疮花属3种,即苣叶秃疮花、秃疮花和宽果秃疮花共11个居群及其姐妹类群海罂粟属1种(海罂粟)一个居群的染色体数目。研究结果表明,秃疮花属全部三种的染色体数目均为2n=12;海罂粟的染色体数目为2n=10,并且是该属中新发现的最小数目。

关键词：秃疮花属海罂粟属罂粟科染色体数目

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Mobilizing Crop Biodiversity

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Molecular Plant 2020年第10期13卷 1341-1344页

作者： Susan McCouch zahra Katy navabi Michael Abberton noelle L.Anglin Rosa Lia Barbieri Michael Baum Kirstin Bett Helen Booker Gerald L.Brown Glenn J.Bryan Luigi Cattivelli David Charest Kellye Eversole Marcelo Freitas Kioumars Ghamkhar Dario Grattipaglia Robert Henry Maria Cleria Valadares Inglis Tofazzal Islam Zakaria Kehel Paul J.Kersey Graham J.King Stephen Kresovich Emily Marden Sean Mayes Marie noelle ndjiondjiop Henry T.nguyen Samuel Rezende Paiva Roberto Papa Peter W.B.Phillips Awais Rasheed Christopher Richards Mathieu Flouard Maria Jose Amstalden Sampaio Uwe Scholz Paul D.Shaw Brad Sherman S.Evan Staton nils Stein Jan Svensson Mark Tester Jose Francisco Montenegro Valls Rajeev Varshney Stephen Visscher Eric von Wettberg Robbie Waugh Peter Wenzl Loren H.Rieseberg Plant Breeding and Genetics School of Integrated Plant SciencesCornell UniversityIthacaNY14853USA DivSeek Global Institute for Food Security110 Gymnasium PlaceUniversity of SaskatchewanSaskatoonSKS7N 0W9Canada lnternational Institute of Tropical Agriculture(IITA) PMB 5320Oyo RdIbadanNigeria International Potato Center(CIP)1895 Avenida La Molina Lima Peru 12Lima 15023Peru Embrapa Genetic Resources and Biotechnology Parque Estacjao BiologicaFinal Av W5 NorteCaixa Postal 0237270770-917-Brasilia DFBrazil International Center for Agricultural Research in the Dry Areas(I CAR DA) Station Exp.INRA-Quich.Rue Hafiane Cherkaoui.Agdal.Rabat-Instituts10111RabatMorocco Department of Plant Sciences University of Saskatchewan51 Campus Dr.SaskatoonSK S7N 5A8Canada Department of Plant Agriculture University of GuelphRm 316Crop Science Bldg50 Stone Rd EGuelphON N1G 2W1Canada Genome Prairie 111 Research DriveSuite 101SaskatoonSKS7N 3R2Canada The James Hutt on Institute Errol RoadInvergowrieDundeeDD25DAUK CREA Research Centre for Genomics and Bioinformaticsvia San Protaso 302Fiorenzuola d'Arda29017Italy Genome British Columbia 400-575 West 8th AvenueVancouverBCV5Z 0C4Canada International Wheat Genome Sequencing Consortium 2841 NE Marywood CtLee's SummitMO.64086USA Forage Science Grasslands Research CentreAgResearchPalmerston North4410New Zealand Queensla nd Alliance for Agriculture and Food Inn ovation University of QueenslandBrisbaneQLD 4072Australia Institute of Biotechnology and Genetic Engineering(IBGE) Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur 1706Bangladesh Southern Cross University PO Box 157LismoreNSW 2480Australia Royal Botanic Gardens KewRichmondSurreyTW93AEUK Feed the Future Innovation Lab for Crop Improvement 431 Weill HallCornell UniversityIthacaNY14853USA Department of Botany and Biodiversity Research Centre University of British ColumbiaVancouverBC V6R 2A5Canada Crops For the Future(UK)CIC 76-80 Baddow Road ChelmsfordEs

Over the past 70 years,the world has witnessed extraordinary growth in crop productivity,enabled by a suite of technological advances,including higher yielding crop varieties,improved farm management,synthetic agrochemicals,and agricultural *** this"Green Revolution"intensified crop production,and is credited with reducing famine and malnutrition,its benefits were accompanied by several undesirable collateral effects(Pingali,2012).These include a narrowing of agricultural biodiversity,stemming from increased monoculture and greater reliance on a smaller number of crops and crop varieties for the majority of our *** reduction in diversity has created vulnerabilities to pest and disease epidemics,climate variation,and ultimately to human health(Harlan,1972).

关键词： intensified crops agricultural

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