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Identification of Panton-Valentine leukocidin virulence gene in methicillin-resistant Staphylococcus aureus isolated from clinical specimens of burn patients in Zare Hospitals of Sari, Iran

引用

Journal of Acute Disease 2020年第3期9卷 121-125页

作者： Atefeh Ranjkesh zahra salari Mehrab Nejati Emad Behboudi Ali Ramezani Milad Zandi Mahmood Marzban Hadis Kiani Mina Bavi Mehdi Rabiei Roodsari Yousef Esvand Zibaee Samaneh Abbasi Maryam Mohammadi Elyasi Mehdi Parsanahad Shahram Jalilian SiminMohammadi Gorji Mina Owrang Faculty of Medicine Sari BranchIslamic Azad UniversitySariIran School of Medicine Bam University of Medical SciencesBamIran Department of Microbiology Faculty of MedicineGolestan University of Medical SciencesGorganIran Virology Department School of MedicineAhvaz Jundishapur University of Medical SciencesAhvazIran Nursing School of Tabas Birjand University of Medical SciencesBirjandIran Department of Virology School of Public HealthTehran University of Medical SciencesTehranIran Department of Laboratory Sciences Borujerd Branch Islamic Azad UniversityBorujerdIran Abadan Faculty of Medical Sciences AbadanIran School of Paramedical Sciences Dezful University of Medical SciencesDezfulIran

Objective:To identify the pathogenic gene of Panton-Valentine leukocidin in methicillin-resistant Staphylococcus aureus strain isolated from clinical specimens of burn patients in Shaheed Zare Burn ***:A total of 104 samples of Staphylococcus were collected and 78 aureus samples were isolated from Zare Hospital patients from November 2016 to July *** isolates were identified using a standard biochemical and laboratory methodology in accordance with CLSI principles,and disk agar diffusion antibiogram were performed to identify methicillin resistant *** the presence of the Panton-Valentine leukocidin gene was tested by ***:Of the methicillin-resistant Staphylococcus aureus samples,80%were negative for the Panton-Valentine leukocidin gene,and only 20%of the samples had Panton-Valentine leukocidin *** and female patients showed no significant difference in the positivity rate of Panton-Valentine leukocidin gene(16.12%vs.33.33%)(P=0.25).Besides,there was no significant difference in bacterial resistance or susceptibility to antibiotics between samples with or without the Panton-Valentine leukocidin ***:In recent years,increased resistance has been a serious *** resistance or susceptibility of Staphylococcus aureus strains to different antibiotics is different in different geographical locations.

关键词： Methicillin Resistance Panton-Valentine leukocidin PCR Staphylococcus aureus

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