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Isolation and Identification of Multi-Drug Resistant Strains of Non-Lactose Fermenting Bacteria from Clinical Isolates

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Open Journal of Medical Microbiology 2014年第2期4卷 115-123页

作者： Minhas Akbar Muhammad Zahid Pir Asmat Ali Aftab Alam Sthanadar Mudassir Shah Iram Alam Sthanadar Muhammad Kaleem Muhammad Aslam Khayyam zahirullah Syeda Mahreen Ul Hassan Noor Jehan Muhammad Ismail Khan Department of Microbiology Shaheed Benazir Bhutto Women University Peshawar Pakistan Department of Zoology Islamia College University Peshawar Pakistan Department of Zoology Islamia College University Peshawar Pakistan Department of Zoology and Animal Sciences Post Graduate College Dargai Malakand Pakistan Department of Zoology Islamia College UniversityPeshawar Pakistan Government Degree College Dara Adam Kheil FR Kohat Pakistan Lady Reading Hospital Peshawar Pakistan

Purpose: We studied the drug resistance of different microbes from clinical isolates. The morphological characteristics of bacteria were observed through culture characteristics and by carrying out gram staining techniques while the biochemical characteristics of bacteria were carried out by biochemical test. Methods: A total of 324 samples were collected from suspected patients visiting different hospitals at district Peshawar. For morphological identification, samples of clinical isolates were analyzed by blood agar, MacConkey agar and Eosine Methylene Blue, identified by gram staining and characterized by different biochemical tests. Antibiotic Sensitivity test by Modified Kirby-Bauer Disc diffusion method was used to test the in-vitro susceptibility of the identified isolates to different antibiotics such as Ceftazidime, Ceftazidime, Ceftriaxone, Cefepime and Imipenem. Results: These resistant non-lactose fermenting gram negative bacteria were isolated from samples of pus/wound (33.30%, n = 108/324), blood (33.30%, n = 108/324), urine (23.30%, n = 75/324) and from ascetic/pleural fluids (10.20%, n = 33/324). The study revealed that the percentage of non-fermenting bacterial infection was higher in females (53%) as compared to males (47%) along with higher infection observed in the age group of 11 - 30 years. Pseudomonas aeroginosa showed high resistance against Cefepime (88.80%), followed by Cefoperazone (55.50%), Ceftazidime (48.10%), Ceftriaxone (33.30%). Imipenem was active with low resistance (7.40%). More resistance was seen in Morganella morganii against Imipenem (66.70%) followed by Cefoperazone (55.50%), Ceftriaxone (55.50%). Cefepime showed low resistance (11%). Multi-drug resistant Proteus mirabillis was highly resistance to Ceftriaxone (74.07%), followed by Cefepime (59.20%), Cefoperazone (44.40%) and low resistance for Imipenem (25.90%). Salmonella typhi demonstrated high resistance against Imipenem (74.07%), followed by Ceftriaxone (40.70%), Ceftazi

关键词： Multidrug Resistant Non-Lactose Fermenting Gram Negative Bacteria Disc Diffusion Technique

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