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Molecular characterization of sweet potato(Ipomoea batatas[L.]Lam)germplasms for desirable traits by using simple sequence repeats markers

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BIOCELL 2023年第1期47卷 227-237页

作者： KHANDAKAR ABUMDMOSTAFIZAR RAHMAN ABDUL SHUKOR JURAIMI MDREZWAN MOLLA MUHAMMAD ASYRAFMD HATTA zulkefly bin sulaiman SHAMIMA SULTANA AHMED GABER BENUKAR BISWAS AKBAR HOSSAIN Tuber Crop Research Centre Bangladesh Agricultural Research InstituteGazipur1701Bangladesh Department of Crop Science Faculty of AgricultureUniversiti Putra MalaysiaUPMKuala Lumpur43400Malaysia Plant Genetic Resources Centre Bangladesh Agricultural Research InstituteGazipur1701Bangladesh Department of Agriculture Technology Faculty of AgricultureUniversiti Putra MalaysiaUPMKuala Lumpur43400Malaysia Regional Agricultural Research Station Bangladesh Agricultural Research InstituteCumilla3500Bangladesh Department of Biology College of ScienceTaif UniversityTaif21944Saudi Arabia Department of Agronomy Bidhan Chandra Krishi ViswavidyalayaNadiaWB741252India Division of Agronomy Bangladesh Wheat and Maize Research InstituteDinajpur5200Bangladesh

Every breeding program that aims to create new and improved cultivars with desired traits mostly relies on information related to genetic ***,molecular characterization of germplasms is important to obtain target cultivars with desirable *** potato[Ipomoea batatas(L.)Lam]is widely considered the world’s most important crop,with great diversity in morphological and phenotypic *** genetic diversity of 20 sweet potato germplasms originating from Bangladesh,CIP,Philippines,Taiwan,and Malaysia were compared,which was accomplished by genetic diversity analysis by exploring 20 microsatellite DNA markers for germplasm characterization and *** information was effective in differentiating or clustering the sweet potato genotypes.A total of 64 alleles were generated using the 20 primers throughout the 20 germplasm samples,with locus IBS97 having the highest number of alleles(5),whereas locus IbU33 had the fewest alleles(2).The alleles varied in size from 105(IbU31)to 213 base pairs(IBS34).The Polymorphism Information Content(PIC)values for the loci IbL46 and IBS97 varied from 0.445 to ***97 has the highest number of effective alleles(3.704),compared to an average of *** average Shannon’s diversity index(H)was 1.003,ranging from 0.673 in IbU3 to 1.432 in *** value of gene flow(Nm)varied between 0.000 and 0.005,with an average of 0.003,whereas genetic differentiation(FST-values)ranged between 0.901 and *** sweet potato germplasm included in this study had a broad genetic ***1 ***9 and SP12 ***18 germplasm pairings had the greatest genetic distance(GD=0.965),while SP1 ***2 germplasm couples had the least genetic diversity(GD=0.093).Twenty genotypes were classified into two groups in the UPGMA dendrogram,with 16 genotypes classified as group“A”and the remaining four genotypes,SP10,SP18,SP19,and SP20,classified as group“B.”According to cluster analysis,the anticipated heterozygosity(gene diversity)of Nei

关键词： Sweet potato Simple sequence repeats(SSRs) Genetic diversity Dendrogram

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An Effective Hybrid Model of ELM and Enhanced GWO for Estimating Compressive Strength of Metakaolin-Contained Cemented Materials

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Computer Modeling in Engineering & Sciences 2024年第5期139卷 1521-1555页

作者： Abidhan Bardhan Raushan Kumar Singh Mohammed Alatiyyah sulaiman Abdullah Alateyah Department of Civil Engineering National Institute of Technology PatnaPatnaIndia Department of Computer Engineering and Applications GLA UniversityMathuraIndia Department of Computer Science College of Sciences and Humanities-AflajPrince Sattam Bin Abdulaziz UniversityAl-KharjSaudi Arabia Department of Computer Science College of Science and ArtsQassim UniversityUnaizahSaudi Arabia

This research proposes a highly effective soft computing paradigm for estimating the compressive strength(CS)of metakaolin-contained cemented *** proposed approach is a combination of an enhanced grey wolf optimizer(EGWO)and an extreme learning machine(ELM).EGWO is an augmented form of the classic grey wolf optimizer(GWO).Compared to standard GWO,EGWO has a better hunting mechanism and produces an optimal *** EGWO was used to optimize the ELM structure and a hybrid model,ELM-EGWO,was *** train and validate the proposed ELM-EGWO model,a sum of 361 experimental results featuring five influencing factors was *** on sensitivity analysis,three distinct cases of influencing parameters were considered to investigate the effect of influencing factors on predictive *** consequences show that the constructed ELM-EGWO achieved the most accurate precision in both training(RMSE=0.0959)and testing(RMSE=0.0912)*** outcomes of the ELM-EGWO are significantly superior to those of deep neural networks(DNN),k-nearest neighbors(KNN),long short-term memory(LSTM),and other hybrid ELMs constructed with GWO,particle swarm optimization(PSO),harris hawks optimization(HHO),salp swarm algorithm(SSA),marine predators algorithm(MPA),and colony predation algorithm(CPA).The overall results demonstrate that the newly suggested ELM-EGWO has the potential to estimate the CS of metakaolin-contained cemented materials with a high degree of precision and robustness.

关键词： Metakaolin-contained cemented materials compressive strength extreme learning machine grey wolf optimizer swarm intelligence uncertainty analysis artificial intelligence

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Antioxidants in aqueous extract of Myristica fragrans(Houtt.) suppress mitosis and cyclophosphamide-induced chromosomal aberrations in Allium cepa ***

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第11期12卷 915-922页

作者： Akeem AKINBORO Kamaruzaman bin MOHAMED Mohd Zaini ASMAWI Shaida Fariza sulaiman Othman Ahmad SOFIMAN School of Biological Sciences Universiti Sains Malaysia School of Pharmaceutical Sciences Universiti Sains Malaysia

In this study, freeze-dried water extract from the leaves of Myristica fragrans （Houtt.） was tested for mutagenic and antimutagenic potentials using the Allium cepa assay. Freeze-dried water extract alone and its combination with cyclophosphamide （CP）（50 mg/kg） were separately dissolved in tap water at 500, 1000, 2000, and 4000 mg/kg. Onions （A. cepa） were suspended in the solutions and controls for 48 h in the dark. Root tips were prepared for microscopic evaluation. 2,2-Diphenyl-l-picrylhydrazyl （DPPH） free radicals＇ scavenging power of the extract was tested using butylated hydroxyanisole （BHA） and butylated hydroxytoluene （BHT） as standards. Water extract of Myristica fragrans scavenged free radicals better than BHA, but worse than BHT. The extract alone, as well as in combination with CP suppressed cell division, and induced chromosomal aberrations that were insignificantly different from the negative control （P≤0.05）. However, cytotoxic and mutagenic actions of CP were considerably suppressed. The observed effects on cell division and chromosomes of A. cepa may be principally connected to the antioxidant properties of the extract. The obtained results suggest mitodepressive and antimutagenic potentials of water extract of the leaves of M. fragrans as desirable properties of a promising anticancer agent.

关键词： Allium cepa Antioxidants Chromosomal aberration Cyclophosphamide Mitotic index

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Offshore Software Maintenance Outsourcing Process Model Validation:A Case Study Approach

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Computers, Materials & Continua 2023年第3期74卷 5035-5048页

作者： Atif Ikram Masita Abdul Jalil Amir bin Ngah Adel sulaiman Muhammad Akram Ahmad Salman Khan Faculty of Ocean Engineering Technology and Informatics University Malaysia TerengganuKuala TerengganuMalaysia Department of Computer Science&Information Technology Faculty of Information TechnologyThe University of LahoreLahore54000Pakistan Department of Computer Science College of Computer Science and Information SystemsNajran UniversityNajranSaudi Arabia Department of Software Engineering Faculty of Information TechnologyThe University of LahoreLahore54000Pakistan

The successful execution and management of Offshore Software Maintenance Outsourcing(OSMO)can be very beneficial for OSMO vendors and the OSMO *** a lot of research on software outsourcing is going on,most of the existing literature on offshore outsourcing deals with the outsourcing of software development *** frameworks have been developed focusing on guiding software systemmanagers concerning offshore software ***,none of these studies delivered comprehensive guidelines for managing the whole process of *** is a considerable lack of research working on managing OSMO from a vendor’s ***,to find the best practices for managing an OSMO process,it is necessary to further investigate such complex and multifaceted phenomena from the vendor’s *** study validated the preliminary OSMO process model via a case study research *** results showed that the OSMO process model is applicable in an industrial setting with few *** industrial data collected during the case study enabled this paper to extend the preliminary OSMO process *** refined version of the OSMO processmodel has four major phases including(i)Project Assessment,(ii)SLA(iii)Execution,and(iv)Risk.

关键词： Offshore outsourcing process model model validation vendor challenges case study

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Assessment of <i>Stevia rebaudiana</i>Bertoni Genotypes via Morpho-Agronomic Traits under Two Light Conditions

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American Journal of Plant Sciences 2018年第7期9卷 1403-1413页

作者： Dhulfiqar Aamer Abdulameer Mohamad bin Osman zulkefly sulaiman Mohd Rafi Yusop Shamsiah Abdullah Parisa Azizi Qusay Abdulhamza Muttaleb Department of Crop Science Faculty of Agriculture Universiti Putra Malaysia Serdang Malaysia Institute of Plantation Studies Universiti Putra Malaysia Serdang Malaysia Laboratory of Food Crops Institute of Tropical Agriculture Universiti Putra Malaysia Serdang Malaysia Faculty of Plantation and Agrotechnology Universiti Teknologi MARA Shah Alam Malaysia Technical Institute of Babel Al-Furat Al-Awsat Technical University Al Hillah Iraq

Stevia rebaudiana Bertoni, a non caloric natural sweetner is currently the available substitute to sugar. It is a genus of about 150 species of herbs and shrubs, a compositae and native to Paraguay. However, stevia is a short day plant with almost a constant day length 12 hours in Malaysia stevia generated to flower early. Therefore, the aim of the study was to investigate the effect of different day length on Stevia rebaudiana genotypes in Malaysia. The experiment was undertaken during December 2016 to April 2017 in the Faculty of Design and Architecture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia. Eight stevia genotypes namely (CHV1, Morita2, MS012, MS012 (4x), SA18, TPU, and hybrids IIUM-F1 and UiTM-H2) were evaluated for the agronomic traits under two conditions: sun light (14 hours) using a factorial experiment. The agronomic performance of Stevia rebaudiana was significantly in long day length (>14 hours) environment comparing with short day length (14 hours) environments.

关键词： Stevia rebaudiana Propagation Sun Light Simulated Light

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Culture and identification of neonatal rat brain-derived neural stem cells

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World Journal of Stem Cells 2023年第6期15卷 607-615页

作者： Qing-Zhong Zhou Xiao-Lan Feng Xu-Feng Jia Nurul Huda binti Mohd Nor Mohd Hezery bin Harun Da-Xiong Feng Wan Aliaa Wan sulaiman Department of Orthopedics The Affiliated Hospital of Southwest Medical UniversityLuzhou 646000Sichuan ProvinceChina Department of Neurology Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaSerdang 43400SelangorMalaysia Department of Radiology The Affiliated Hospital of Southwest Medical UniversityLuzhou 646000Sichuan ProvinceChina Department of Orthopedics The Peoples’Hospital of Jianyang CityJianyang 641400Sichuan ProvinceChina Department of Human Anatomi Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaSerdang Selangor43400Malaysia Department of Orthopedics Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaSerdang 43400SelangorMalaysia

BACKGROUND Timing of passaging,passage number,passaging approaches and methods for cell identification are critical factors influencing the quality of neural stem cells(NSCs)*** to effectively culture and identify NSCs is a continuous interest in NSCs study while these factors are comprehensively *** To establish a simplified and efficient method for culture and identification of neonatal rat brain-derived *** First,curved tip operating scissors were used to dissect brain tissues from new born rats(2 to 3 d)and the brain tissues were cut into approximately 1 mm^(3)*** the single cell suspension through a nylon mesh(200-mesh)and culture the sections in *** was conducted with TrypLTM Express combined with mechanical tapping and pipetting ***,identify the 5th generation of passaged NSCs as well as the revived NSCs from *** incorporation method was used to detect self-renew and proliferation capabilities of *** NSCs specific antibodies(anti-nestin,NF200,NSE and GFAP antibodies)were used to identify NSCs specific surface markers and muti-differentiation capabilities by immunofluorescence *** Brain derived cells from newborn rats(2 to 3 d)proliferate and aggregate into spherical-shaped clusters with sustained continuous and stable *** BrdU was incorporated into the 5th generation of passaged cells,positive BrdU cells and nestin cells were observed by immunofluorescence *** induction of dissociation using 5%fetal bovine serum,positive NF200,NSE and GFAP cells were observed by immunofluorescence *** This is a simplified and efficient method for neonatal rat brain-derived neural stem cell culture and identification.

关键词： Neonatal rats Brain-derived neural stem cells Culture Identification

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Secure Communication Scheme based on A New Hyperchaotic System

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Computers, Materials & Continua 2022年第10期73卷 1019-1035页

作者： Khaled Benkouider Aceng Sambas Ibrahim Mohammed sulaiman Mustafa Mamat Kottakkaran Sooppy Nisar Non Destructive Testing Laboratory Automatic DepartmentJijel UniversityBP.9818000JijelAlgeria Department of Mechanical Engineering Universitas Muhammadiyah TasikmalayaTasikmalayaWest Java46196Indonesia School of Quantitative Sciences College of Art and SciencesUniversiti Utara MalaysiaSintok06010KedahMalaysia Faculty of Informatics and Computing Universiti Sultan Zainal AbidinBesut Campus22200Kuala BesutTerengganuMalaysia Department of Mathematics College of Arts and SciencesPrince Sattam bin Abdulaziz UniversityWadi Aldawaser11991Saudi Arabia

This study introduces a new continuous time differential system,which contains ten terms with three quadratic *** new system can demonstrate hyperchaotic,chaotic,quasi-periodic,and periodic behaviors for its different parameter *** theoretical and numerical analysis are investigated to confirm the complex hyperchaotic behavior of our proposed model using many tools that include Kaplan-Yorke dimension,equilibrium points stability,bifurcation diagrams,and Lyapunov *** means of Multisim software,the authors also designed an electronic circuit to confirm our proposed systems’physical *** and Multisim simulation results excellently agree with each other,which validate the feasibility of our new ten terms hyperchaotic system and make it very desirable to use in different domains especially in chaotic-based ***,by employing the drive response synchronisation,we developed a secure communication strategy for the proposed *** from the proposed scheme show that the proposed approach was successful in completing the encryption and decryption procedure.

关键词： Hyperchaotic system synchronization circuit design secure communication system

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Phytochemical Determination and Antibacterial Activity of Punica granatum Peel Extracts against Plant Pathogenic Bacteria

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American Journal of Plant Sciences 2016年第1期7卷 159-166页

作者： Ayad Ismael Khaleel Kamaruzaman Sijam Tavga sulaiman Rashid Khairulmazmi bin Ahmad Plant Protection Department Faculty of Agriculture Universiti Putra Malaysia Serdang Malaysia

Plant pathogenic bacteria are recognized to be harmful microbes able to decrease the quantity and quality of crop production in the world. Punica granatum peel was screened for its potential use as biological control agent for plant pathogenic bacteria. P. granatum peel was successfully extract using n-hexane, methanol and ethyl acetate by maceration. The highest yield obtained by ethyl acetate showed that ethyl acetate extracted more compounds that readily soluble to methanol and n-hexane. For in-vitro antibacterial activity, three different species of plant pathogenic bacteria were used namely Erwinia carotovorum subsp. Carotovorum, Ralstonia solanacearum, and Xanthomonas gardneri. For all crude extracts, four different concentrations 25, 50, 100 and 200 mg/ml were used in cup-plate agar diffusion method. Streptomycin sulfate at concentration 30 μg/ml was used as positive control while each respective solvent used for peel extraction was used as negative control. The results obtained from in vitro studies showed only ethyl acetate extract possessed antibacterial activity tested on the plant pathogenic bacteria. Methanol and n-hexane did not show any antibacterial activity against plant pathogenic bacteria selected where no inhibition zones were recorded. R. solanacearum recorded the highest diameter of inhibition zones for all range of concentrations introduced followed by E. carotovorum subsp. Carotovorum and X. gardneri. For the minimum inhbitory concentration (MIC) and minimum bactericidal concentration (MBC), only the ethyl acetate extract was subjected to the assay as only ethyl acetate extract exhibited antibacterial activity. The minimum concentration of ethyl acetate extract that was able to inhibit plant pathogenic bacteria was recorded at a concentration of 3.12 mg/ml which inhibited R. solancearum and E. carotovorum subsp. Carotovorum, followed by X. gardneri at concentration 6.25 mg/ml. For the minimum bactericidal concentration (MBC), the results show

关键词： Punica granatum Plant Extraction Pathogenic Bacteria Phytochemical Screening

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Gc-Ms Analysis and Antibacterial Activity of Mangosteen Leaf Extracts against Plant Pathogenic Bacteria

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American Journal of Plant Sciences 2016年第7期7卷 1013-1020页

作者： Qamar Mohammed Naji Alsultan Kamaruzaman Sijam Tavga sulaiman Rashid Khairulmazmi bin Ahmad Department of Plant Protection Faculty of Agriculture University Putra Malaysia UPM Serdang Malaysia

The potential of Garcinia mangostana as a biological control agent against plant pathogenic bacteria which decrease the quality and volume of crop production worldwide was assessed. Mangosteen leaves were extracted by maceration using chloroform, n-hexane, and methanol. For the in vitro antibacterial activity, two dissimilar species of plant pathogenic bacteria: Pseudomonas syringe pv. tomato and Xanthomonas oryzae pv. oryzae were acquired. Four different concentrations, 12.5, 25, 50, and 100 mg/ml were obtained through the cup-plate agar diffusion technique. Streptomycin sulphate at 30 μg/ml concentration was set as the positive control, whereas every respective solvent used in the leaf extraction was set as the negative control. The results have shown that, only methanol extract demonstrated antibacterial activity when tested on the plant pathogenic bacteria. The highest diameter of inhibition zones was observed in X. oryzae pv. oryzae, at all range of concentrations, followed by P. syringae pv. tomato. The least methanol extract concentration utilised in determination of minimum inhibitory concentration (MIC) assay was at 1.562 mg/ml, inhibiting X. oryzae pv. oryzae, followed by P. syringe pv. tomato at a concentration 3.125 mg/ml. Antibacterial impacts of the most effectual extract of mangosteen crude were supported by the existence of chemical components identified by GC-MS. Cycloartenol, Caryophyllene, Docosane, Phenol, 4,4-Methylenebis (2,6-di-tert-butylphenol) and Chromium were noted as key compounds in the mangosteen leaf extract, which were perhaps causing the antibacterial activity.

关键词： Pseudomonas syringae Xanthomonas oryzae Garcinia mangostana and Methanol Extract

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Performance Framework for Virtual Machine Migration in Cloud Computing

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Computers, Materials & Continua 2023年第3期74卷 6289-6305页

作者： Tahir Alyas Taher M.Ghazal Badria sulaiman Alfurhood Munir Ahmad Ossma Ali Thawabeh Khalid Alissa Qaiser Abbas Department of Computer Science Lahore Garrison UniversityLahore54000Pakistan School of Information Technology Skyline University CollegeUniversity City SharjahSharjah1797UAE Center for Cyber Security Faculty of Information Science and TechnologyUniversiti Kebansaan Malaysia(UKM)Bangi43600SelangorMalaysia Department of Computer Sciences College of Computer and Information SciencesPrincess Nourah Bint Abdulrahman UniversitySaudi Arabia School of Computer Science National College of Business Administration&EconomicsLahore54000Pakistan Dean of Student Affairs Skyline University CollegeUniversity City SharjahSharjah1797UAE Networks and Communications Department College of Computer Science and Information TechnologyImam Abdulrahman Bin Faisal UniversityDammam31441Saudi Arabia Faculty of Computer and Information Systems Islamic University Madinah Madinah42351Saudi Arabia

In the cloud environment,the transfer of data from one cloud server to another cloud server is called *** can be delivered in various ways,from one data centre to *** research aims to increase the migration performance of the virtual machine(VM)in the cloud *** allow cloud customers to store essential data and ***,server usage has grown dramatically due to the virtualization of computer systems,resulting in higher data centre power consumption,storage needs,and operating *** VMs on one data centre manage share resources like central processing unit(CPU)cache,network bandwidth,memory,and application ***-cloud,VMmigration addresses the performance degradation due to cloud server configuration,unbalanced traffic load,resource load management,and fault situations during data *** speed is influenced by the size of the VM,the dirty rate of the running application,and the latency ofmigration *** a result,evaluating VM migration performance while considering all of these factors becomes a difficult *** effort of this research is to assess migration problems on *** simulation results in Matlab show that if the VMsize grows,themigration time of VMs and the downtime can be impacted by three orders *** dirty page rate decreases,themigration time and the downtime grow,and the latency time decreases as network bandwidth increases during the migration time and post-migration overhead calculation when the VMtransfer is *** the simulated cases of VMs migration were performed in a fuzzy inference system with performance graphs.

关键词： Latency cloud computing dirty page ratio storage migration performance

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