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Long non-coding RNA H19 regulates neurogenesis of induced neural stem cells in a mouse model of closed head injury

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Neural Regeneration Research 2024年第4期19卷 872-880页

作者： Mou Gao Qin Dong zhijun Yang Dan zou Yajuan Han Zhanfeng Chen Ruxiang Xu Department of Neurosurgery Sichuan Academy of Medical Sciences and Sichuan Provincial People's HospitalSchool of MedicineUniversity of Electronic Science and Technology of China Department of Neurosurgery Chinese PLA General Hospital Zhongsai Stem Cell Genetic Engineering Co. Ltd. Department of Neurology Fu Xing HospitalCapital Medical University

Stem cell-based therapies have been proposed as a potential treatment for neural regeneration following closed head injury.We previously reported that induced neural stem cells exert beneficial effects on neural regeneration via cell replacement.However,the neural regeneration efficiency of induced neural stem cells remains limited.In this study,we explored differentially expressed genes and long non-coding RNAs to clarify the mechanism underlying the neurogenesis of induced neural stem cells.We found that H19 was the most downregulated neurogenesis-associated lnc RNA in induced neural stem cells compared with induced pluripotent stem cells.Additionally,we demonstrated that H19 levels in induced neural stem cells were markedly lower than those in induced pluripotent stem cells and were substantially higher than those in induced neural stem cell-derived neurons.We predicted the target genes of H19 and discovered that H19 directly interacts with mi R-325-3p,which directly interacts with Ctbp2 in induced pluripotent stem cells and induced neural stem cells.Silencing H19 or Ctbp2 impaired induced neural stem cell proliferation,and mi R-325-3p suppression restored the effect of H19 inhibition but not the effect of Ctbp2 inhibition.Furthermore,H19 silencing substantially promoted the neural differentiation of induced neural stem cells and did not induce apoptosis of induced neural stem cells.Notably,silencing H19 in induced neural stem cell grafts markedly accelerated the neurological recovery of closed head injury mice.Our results reveal that H19 regulates the neurogenesis of induced neural stem cells.H19 inhibition may promote the neural differentiation of induced neural stem cells,which is closely associated with neurological recovery following closed head injury.

关键词： closed head injury Ctbp2 induced neural stem cell lncRNA H19 miR-325-3p neurogenesis

Induced neural stem cells regulate microglial activation through Akt-mediated upregulation of CXCR4 and Crry in a mouse model of closed head injury

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Neural Regeneration Research 2025年第5期20卷 1416-1430页

作者： Mou Gao Qin Dong Dan zou zhijun Yang Lili Guo Ruxiang Xu Department of Neurosurgery Sichuan Academy of Medical Sciences and Sichuan Provincial People’s HospitalSchool of MedicineUniversity of Electronic Science and Technology of ChinaChengduSichuan ProvinceChina Department of Neurosurgery Chinese PLA General HospitalBeijingChina Department of Neurology Fu Xing HospitalCapital Medical UniversityBeijingChina

Microglial activation that occurs rapidly after closed head injury may play important and complex roles in neuroinflammation-associated neuronal damage and repair.We previously reported that induced neural stem cells can modulate the behavior of activated microglia via CXCL12/CXCR4 signaling,influencing their activation such that they can promote neurological recovery.However,the mechanism of CXCR4 upregulation in induced neural stem cells remains unclear.In this study,we found that nuclear factor-κB activation induced by closed head injury mouse serum in microglia promoted CXCL12 and tumor necrosis factor-αexpression but suppressed insulin-like growth factor-1 expression.However,recombinant complement receptor 2-conjugated Crry(CR2-Crry)reduced the effects of closed head injury mouse serum-induced nuclear factor-κB activation in microglia and the levels of activated microglia,CXCL12,and tumor necrosis factor-α.Additionally,we observed that,in response to stimulation(including stimulation by CXCL12 secreted by activated microglia),CXCR4 and Crry levels can be upregulated in induced neural stem cells via the interplay among CXCL12/CXCR4,Crry,and Akt signaling to modulate microglial activation.In agreement with these in vitro experimental results,we found that Akt activation enhanced the immunoregulatory effects of induced neural stem cell grafts on microglial activation,leading to the promotion of neurological recovery via insulin-like growth factor-1 secretion and the neuroprotective effects of induced neural stem cell grafts through CXCR4 and Crry upregulation in the injured cortices of closed head injury mice.Notably,these beneficial effects of Akt activation in induced neural stem cells were positively correlated with the therapeutic effects of induced neural stem cells on neuronal injury,cerebral edema,and neurological disorders post–closed head injury.In conclusion,our findings reveal that Akt activation may enhance the immunoregulatory effects of induced neural stem cells on microglial activation via upregu

关键词： Akt signaling cerebral edema closed head injury Crry CXCR4 induced neural stem cell microglia neuroinflammation

四川盆地南缘昭通示范区罗布向斜志留系龙马溪组页岩储层裂缝建模

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岩性油气藏 2024年第3期36卷 137-145页

作者：计玉冰郭冰如梅珏尹志军邹辰中国石油浙江油田公司勘探开发一体化中心杭州310023 中国石油大学(北京)地球科学学院北京102249

基于岩心分析、成像测井分析和三维地震资料,对四川盆地南缘昭通示范区罗布向斜龙一1亚段和五峰组的多尺度离散裂缝网络建模方法进行了研究。研究结果表明:(1)将蚂蚁体属性中的强响应部分确定性提取成面,可得到大尺度离散裂缝网络模型;... 详细信息

基于岩心分析、成像测井分析和三维地震资料,对四川盆地南缘昭通示范区罗布向斜龙一1亚段和五峰组的多尺度离散裂缝网络建模方法进行了研究。研究结果表明:(1)将蚂蚁体属性中的强响应部分确定性提取成面,可得到大尺度离散裂缝网络模型;用蚂蚁体沿层切片显示的带走向趋势的裂缝参与小尺度离散裂缝网络建模,降低了小尺度裂缝走向模拟的不确定性。(2)罗布向斜龙一1亚段和五峰组裂缝以高角度剪切缝为主,多被方解石充填,裂缝走向为北东—南西向,成像测井解释的裂缝走向与附近地震解释的断层走向一致。(3)大尺度离散裂缝网络模型与钻井的断层位置相符,小尺度裂缝发育部位与钻井液漏失部位吻合,多尺度离散裂缝网络模型可较好地表征罗布向斜龙一1亚段和五峰组的裂缝发育特征。(4)罗布向斜Y12H井应用多尺度离散裂缝网络模型优化压裂方案,压裂缝网复杂度高,试气产量较邻井提高了近41%,优化小尺度裂缝走向后的多尺度离散裂缝网络模型实际应用效果较好。

关键词：多尺度裂缝建模离散裂缝网络模型蚂蚁体页岩储层龙马溪组志留系罗布向斜昭通示范区四川盆地

Pet-keeping and its impact on asthma and allergies among preschool children in Shanghai, China

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Chinese Science Bulletin 2013年第34期58卷 4203-4210页

作者： HUANG Chen HU Yu LIU Wei zou zhijun SUNDELL Jan School of Environment and Architecture University of Shanghai for Science and Technology Department of Building Science Tsinghua University

Whether early pet-keeping is a risk factor for children’s asthma and allergies remains controversial.To investigate associations between asthma,allergies and airway symptoms among children and the indoor environment,a cross-sectional study was conducted in 5 districts of Shanghai.A number of 13335 questionnaires(response rate:85.3%)of 4–6 year old children were analyzed.Families in urban areas have more pets except for dogs than families in suburbs.Fish are the most common pets in urban districts.The prevalence of doctor-diagnosed asthma was 10.3%,wheeze(ever)28.3%,rhinitis(ever)54.1%,doctor-diagnosed hay fever 12.6%and eczema(ever)22.9%.In logistic regression analyses,early furred pet-keeping was positively associated with most of the symptoms and significantly with rhinitis(ever,adjusted OR=1.41,95%CI=1.14–1.76)and doctor-diagnosed hay fever(1.38,1.02–1.88).Current furred pet-keeping was significantly negatively associated with doctor-diagnosed asthma(0.57,0.39–0.83).Persistent furred pet-keeping was significantly positively associated with rhinitis on pet or pollen exposure.However,current pet-keeping is not randomly distributed in the population.Children in families with"allergy"or with"pet avoidance behavior"(due to allergies in the family)have more symptoms,but have avoided cats and dogs,leading to the conclusion that such animals are"protective",namely the"Healthy Pet-Keeping"effect.Moreover,rodents and birds are risks for children’s health.Fish-keeping is also seemingly a risk.This study indicates that early pet-keeping is a risk factor for asthma and allergies in families with a history of allergies,and part of residents in Shanghai have pet-avoidance behavior.

关键词：学龄前儿童过敏症哮喘宠物上海 Logistic回归分析呼吸道症状饲养

Associations between indoor environmental smoke and respiratory symptoms among preschool children in Shanghai, China

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Chinese Science Bulletin 2013年第34期58卷 4211-4216页

作者： LIU Wei HUANG Chen HU Yu zou zhijun SUNDELL Jan School of Environment and Architecture University of Shanghai for Science and Technology Department of Building Science Tsinghua University

Whether indoor environmental smoke is harmful for preschool children’s respiratory health in a society where female smoking is rare has not been determined.This study is part of a cross-sectional study(CCHH study-phase one in Shanghai)and investigated associations between parental smoking and incense-burning and respiratory symptoms among 4–6 year old children in Shanghai,China.A number of 13335 valid questionnaires(response rate:85.3%)were analyzed.A number of 56.1%(as reported by a parent)of preschool children in Shanghai are exposed to environmental tobacco smoke(ETS).A number of 40.3%of fathers and0.9%of mothers are smokers.A number of 53.7%and 12.6%of Shanghai residents have used mosquito-repellent incense and incensation respectively.Children exposed to any parental smoking have higher prevalence of wheeze and croup than those not exposed.Current maternal smoking has a significant and positive association with wheeze(in the last 12 months,AOR,95%CI:1.83,1.11–2.99).However,paternal smoking either currently or at child’s birth had only weak associations with wheeze and croup.Incense-burning(mosquito-repellent incense and incensation)had significant and negative association with doctor-diagnosed asthma(AOR,95%CI:0.85,0.73–0.99)and hay fever(AOR,95%CI:0.80,0.70–0.93).The results indicate that maternal smoking perhaps is a stronger risk factor for children’s respiratory health than paternal smoking.

关键词：学龄前儿童呼吸道症状室内环境烟草烟雾弱关联上海中国呼吸系统健康

船用空调阻性消声器消声量计算方法优化的实验研究

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洁净与空调技术 2024年第2期 15-19页

作者：王盛刁永发邹志军东华大学上海理工大学

在船用空调系统中,由于已有的阻性消声器消声量的经验公式忽视了高频失效效应的影响,使得原有的消声量计算方法在高频段不再适用,为了探究出一个符合船用空调阻性消声器消声量的计算公式,通过分析实验数据,选择一个与模型相适应的经验公... 详细信息

在船用空调系统中,由于已有的阻性消声器消声量的经验公式忽视了高频失效效应的影响,使得原有的消声量计算方法在高频段不再适用,为了探究出一个符合船用空调阻性消声器消声量的计算公式,通过分析实验数据,选择一个与模型相适应的经验公式,最终在此基础上结合实际工况对经验公式进行修正与检验。结果表明:当频率处于中低频时,罗杰斯公式的消声量计算值与插入损失值的变化趋势十分接近,误差在4dB左右,但在高频段,由于高频失效效应,经验公式不再适用;其次拟合出来的经验公式修正式,在倍频程下的消声量值与实际工况相互一致,误差低于3dB,满足实验预期要求。

关键词：阻性消声器消声系数插入损失消声量经验公式

In situ kinetics and flocs conformation studies of kaolinite flocculated by Chi-g-CPAM

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International Journal of Minerals,Metallurgy and Materials 2023年第5期30卷 813-823页

作者： Ruijing Xu Wenjie zou Bo Rao Wei Zhao Ting Wang zhijun Zhang State Key Laboratory of High-Efficient Mining and Safety of Metal Mines of Ministry of Education University of Science and Technology BeijingBeijing 100083China School of Civil and Resources Engineering University of Science and Technology BeijingBeijing 100083China School of Chemical and Environmental Engineering China University of Mining and TechnologyBeijing 100083China

This study presents a comparative study of the flocculation behavior of kaolinite induced by chitosan-graft-poly(acrylamide-dimethyl diallyl ammonium chloride)(Chi-g-CPAM) and a commercial cationic polyacrylamide(CPAM).The flocculation behaviour was characterised in terms of both flocculation kinetics and the corresponding morphology changes during flocculation.Both Chi-g-CPAM and CPAM were grafted from silica wafers by means of atom transfer radical polymerization(ATRP).The quartz crystal microbalance with dissipation(QCM-D) tests were conducted.The equilibrium time flocculated by Chi-g-CPAM was found to be 0.46 times as that of CPAM,together with a larger total mass of kaolinite layer.The flocculation behaviour by Chi-g-CPAM can be well captured by a pseudo-first-order model.In contrast,the presence of CPAM leads to a more complex kinetics.A relatively larger fitting slope(0.4663) was obtained at the initial stage but the fitting slope droped to 0.2026 after 800 min,indicating a densification process caused by CPAM.The flocculation kinetics of CPAM can be captured by the Elovich model for the inital stage but the combination of pseudo-first-order and pseudo-second-order models for the latter stages,which can be attributed to the long chain of CPAM.With a dosage of 75 g/t,the settling test with Chi-g-CPAM exhibits the same turbidity in the supernatant but a smaller layer thickness of the settlement compared to CPAM.The study enables a better understanding of the flocculation behavior and contributes to the development of efficient flocculants in mineral processing and tailings treatment.

关键词： Chi-g-CPAM flocculation QCM-D kinetics conformation

Flexible piezoelectric PVDF nanofiber film sensor by blow spinning

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Science China(Technological Sciences) 2023年第3期66卷 863-868页

作者： HE Wei DAI zhijun zou KangKang LI XueYing HAO SongHua WANG HaoLun School of Aeronautics and Astronautics Sichuan UniversityChengdu 610065China

Flexible sensors can be widely applied in the area of lightweight,wearable and intelligent electronic devices.In aerospace,for example,flexible sensors are suitable for measuring pressure in complex aircraft structures.In recent years,flexible sensors have been well developed;however,most of them lack circuit parts,meaning that the sensors have to be connected to an external electrical source.Additionally,the current traditional design limits their flexibility.Thus,this article proposes a self-powered flexible sensor based on PVDF nanofiber film,which is created through an efficient blow spinning method.The piezoelectric PVDF nanofiber films were prepared,and the flexible pressure sensor was assembled.The PVDF nanofiber film sensor showed good sensitivity and stability,and it can be applied for the detection of human physiological signals.

关键词： PVDF nanofiber network blow spinning flexible sensor

人工湿地对水体中TDS、Turb及FDOM的净化效果研究

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中南林业调查规划 2024年第2期43卷 58-63页

作者：邹雨函刘志君谢思妤李伟中国林业科学研究院湿地研究所北京100091 北京一零一中学北京100080 北京汉石桥湿地生态系统国家定位观测研究站北京101300

以北京市野生动物救护中心的人工湿地为案例,评估人工湿地作为一种生态净化系统在不同环境条件下对水体中溶解性总固体(TDS)、浊度(Turb)和荧光溶解性有机质(FDOM)的去除效果。采用定位观测与对比分析方法,于2023年5月至7月期间,在北京... 详细信息

以北京市野生动物救护中心的人工湿地为案例,评估人工湿地作为一种生态净化系统在不同环境条件下对水体中溶解性总固体(TDS)、浊度(Turb)和荧光溶解性有机质(FDOM)的去除效果。采用定位观测与对比分析方法,于2023年5月至7月期间,在北京市野生动物救护中心人工湿地选取不同的样点进行水样采集与监测,使用YSI6820多参数水质监测仪监测TDS、Turb及FDOM的浓度变化,并与2009年同期数据进行比较。研究结果表明:随着样点距离的增加,人工湿地对TDS、Turb和FDOM等污染物的去除率呈上升趋势,且同一样点不同月份之间的去除率存在差异;与2009年人工湿地浊度去除率对比分析可知,2023年浊度去除率显著提升;水体温度的升高能够提高人工湿地对TDS、Turb及FDOM的去除率。

关键词：人工湿地水体净化去除率 TDS Turb FDOM