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High-yielding cultivation techniques for hybrid rice Xieyou 46

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Rice Science 1991年第3期 4-4页

作者： FEI Huailin JIN Qianyu XU Dehai CNRRI,hangzhou 310006,china zou qingdi zhejiang provincial bureau of agriculture,hangzhou 310009,china

High-yielding cultivation techniques for hybridrice Xieyou 46 newly bred by CNRRI were studiedwhen it was grown as a 2nd crop of rice in 1988 inLongyou County,zhejiang *** results areshown as follows:Suitable yield components According to thedata from investigating 43 high-yielding test sitesand the results of the authors' experiments con-

关键词： High-yielding cultivation techniques for hybrid rice Xieyou 46 high

Coated sodium butyrate ameliorates high‑energy and low‑protein diet induced hepatic dysfunction via modulating mitochondrial dynamics, autophagy and apoptosis in laying hens

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Journal of Animal Science and Biotechnology 2024年第3期15卷 1190-1206页

作者： Sasa Miao Tianming Mu Ru Li Yan Li Wenyan Zhao Jiankui Li Xinyang Dong Xiaoting zou Key Laboratory of Animal Feed and Nutrition of zhejiang Province Key Laboratory of Animal Nutrition and Feed Science(Eastern of China)Ministry of Agriculture and Rural AffairsThe Key Laboratory of Molecular Animal NutritionMinistry of EducationCollege of Animal SciencesZhejiang UniversityHangzhou 310058China.

Background Fatty liver hemorrhagic syndrome(FLHS),a fatty liver disease in laying hens,poses a grave threat to the layer industry,stemming from its ability to trigger an alarming plummet in egg production and usher in acute mortality among laying *** evidence suggests that the onset and progression of fatty liver was closely related to mitochondria *** butyrate was demonstrated to modulate hepatic lipid metabolism,alle-viate oxidative stress and improve mitochondrial dysfunction in vitro and mice ***,there is limited existing research on coated sodium butyrate(CSB)to prevent FLHS in laying hens,and whether and how CSB exerts the anti-FLHS effect still needs to be *** this experiment,the FLHS model was induced by administering a high-energy low-protein(HELP)diet in laying *** objective was to investigate the effects of CSB on alleviating FLHS with a focus on the role of CSB in modulating mitochondrial *** A total of 288 healthy 28-week-old Huafeng laying hens were arbitrarily allocated into 4 groups with 6 replicates each,namely,the CON group(normal diet),HELP group(HELP diet),CH500 group(500 mg/kg CSB added to HELP diet)and CH750 group(750 mg/kg CSB added to HELP diet).The duration of the trial encompassed a period of 10 *** The result revealed that CSB ameliorated the HELP-induced FLHS by improving hepatic steatosis and patho-logical damage,reducing the gene levels of fatty acid synthesis,and promoting the mRNA levels of key enzymes of fatty acid *** reduced oxidative stress induced by the HELP diet,upregulated the activity of GSH-Px and SOD,and decreased the content of MDA and *** also mitigated the HELP diet-induced inflammatory response by blocking TNF-α,IL-1β,and F4/*** addition,dietary CSB supplementation attenuated HELP-induced activation of the mitochondrial unfolded protein response(UPRmt),mitochondrial damage,and decline of ATPase *** diet decreased th

关键词： Autophagy Coated sodium butyrate Laying hens Lipid metabolism Mitochondria

Effects of metabolizable energy and crude protein levels on laying performance,egg quality and serum biochemical indices of Fengda-1 layers

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Animal Nutrition 2016年第2期2卷 93-98页

作者： Yang Ding Xingchen Bu Nannan Zhang Lanlan Li Xiaoting zou Key Laboratory of Animal Nutrition and Feed Science Ministry of Agriculture Zhejiang Provincial Laboratory of Feed and Animal Nutrition Feed Science Institute Zhejiang University

This study was conducted to investigate the effects of dietary ME and CP levels on laying performance, egg quality and serum biochemical indices of Fengda-1 layers. In a 2 × 3 factorial arrangement, 2,400 Fengda-1 layers(32 wk of age) were randomly assigned to 6 experimental diets with 2,650 and 2,750 kcal of ME/kg of diet, each containing 14.50%, 15.00% and 15.50% CP, respectively. Each dietary treatment was replicated 5 times, and feed and water were provided ad libitum. The trial lasted for 10 wk, including a2-week acclimation period and an 8-week experimental period. Our results showed that ADFI decreased as the ME level of diet increased from 2,650 to 2,750 kcal/kg(P 0.05). Based on the data under the experimental conditions, the optimal dietary ME and CP levels of Fengda-1 layers are 2,650 kcal/kg and 15.00%(33 to 41 wk of age).

关键词： Energy Protein Laying performance Egg quality Fengda-1 layers

Graphitic carbon nitride nanosheets mitigate cadmium toxicity in Glycine max *** promoting cadmium retention in root and improving photosynthetic performance

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Journal of Environmental Sciences 2024年第5期139卷 543-555页

作者： Kai Xu Yunyu Guo Chenghua Xing Ronglong Fu Bin zou Rongchuan Liu Luyi Cai Jianfang Yan Xi-Lin Wu Miaozhen Cai College of Geography and Environmental Sciences Zhejiang Normal UniversityJinhua 321004China College of agriculture Jinhua PolytechnicJinhua 321007China

Cadmium(Cd)pollution poses a serious threat to plant growth and *** have shown great application potential for alleviation of Cd toxicity to *** this study,we applied graphitic carbon nitride nanosheets(g-C_(3)N_(4)NSs)for alleviation of Cd-toxicity to soybean(Glycine max L.).The g-C_(3)N_(4)NSs supplementation significantly improved plant growth and reduced oxidative damage in the Cd-toxicated soybean seedlings through hydroponic ***,the g-C_(3)N_(4)NSs dynamically regulated the root cell wall(RCW)components by increasing pectin content and modifying its demethylation via enhancing pectin methylesterase(PME)activity,therefore greatly enhanced stronger RCW-Cd retention(up to 82.8%)and reduced Cd migration to the ***,the g-C_(3)N_(4)NSs reversed the Cd-induced chlorosis,increased photosynthetic efficiency because of enhancement in F_v/F_mration,Y(Ⅱ)and sugars *** results provide new insights into the alleviation of Cd toxicity to plants by g-C_(3)N_(4)NSs,and shed light on the application of low-cost and environmental-friendly carbon-based NMs for alleviating heavy metal toxicity to plants.

关键词： Cadmium Carbon nitride nanosheets Cell wall Pectin Pectin methylesterase Soybean

Cynaroside regulates the AMPK/SIRT3/Nrf2 pathway to inhibit doxorubicin-induced cardiomyocyte pyroptosis

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Journal of zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第9期25卷 756-772页

作者： Hai zou Mengyu ZHANG Xue YANG Huafeng SHOU Zhenglin CHEN Quanfeng ZHU Ting LUO Xiaozhou MOU Xiaoyi CHEN Department of Critical Care Medicine Fudan University Shanghai Cancer CenterShanghai200032China Department of Oncology Shanghai Medical CollegeFudan UniversityShanghai200032China Xianghu Laboratory Hangzhou311231China Clinical Research Institute Key Laboratory of Tumor Molecular Diagnosis and Individualized Medicine of Zhejiang ProvinceZhejiang Provincial People’s Hospital(Affiliated People’s Hospital)Hangzhou Medical CollegeHangzhou310014China Center for Reproductive Medicine Department of GynecologyZhejiang Provincial People’s Hospital(Affiliated People’s Hospital)Hangzhou Medical CollegeHangzhou310014China Graduate School of zhejiang Chinese Medical University Hangzhou310053China State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Laboratory(Hangzhou)for Risk Assessment of Agricultural Products of Ministry of AgricultureInstitute of Agro-product Safety and NutritionZhejiang Academy of Agricultural SciencesHangzhou310021China General Surgery Cancer CenterDepartment of Hepatobiliary&Pancreatic Surgery and Minimally Invasive SurgeryZhejiang Provincial People’s Hospital(Affiliated People’s Hospital)Hangzhou Medical CollegeHangzhou310014China

Doxorubicin(DOX)is a commonly administered chemotherapy drug for treating hematological malignancies and solid tumors;however,its clinical application is limited by significant ***(Cyn)is a flavonoid glycoside distributed in honeysuckle,with confirmed potential biological functions in regulating inflammation,pyroptosis,and oxidative ***,the effects of Cyn were evaluated in a DOX-induced cardiotoxicity(DIC)mouse model,which was established by intraperitoneal injections of DOX(5 mg/kg)once a week for three *** mice in the treatment group received dexrazoxane,MCC950,and Cyn every two *** biochemistry,histopathology,immunohistochemistry,reverse transcription-quantitative polymerase chain reaction(RT-qPCR),and western blotting were conducted to investigate the cardioprotective effects and potential mechanisms of Cyn *** results demonstrated the significant benefits of Cyn treatment in mitigating DIC;it could effectively alleviate oxidative stress to a certain extent,maintain the equilibrium of cell apoptosis,and enhance the cardiac function of *** effects were realized via regulating the transcription levels of pyroptosis-related genes,such as nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3),caspase-1,and gasdermin D(GSDMD).Mechanistically,for DOX-induced myocardial injury,Cyn could significantly modulate the expression of pivotal genes,including adenosine monophosphate-activated protein kinase(AMPK),peroxisome proliferator-activated receptorγcoactivator-1α(PGC-1α),sirtuin 3(SIRT3),and nuclear factor erythroid 2-related factor 2(Nrf2).We attribute it to the mediation of AMPK/SIRT3/Nrf2 pathway,which plays a central role in preventing DOX-induced cardiomyocyte *** conclusion,the present study confirms the therapeutic potential of Cyn in DIC by regulating the AMPK/SIRT3/Nrf2 pathway.

关键词： Cynaroside Doxorubicin Pyroptosis Cardiotoxicity Oxidative stress

COP9 signalosome subunit PfCsnE regulates secondary metabolism and conidial formation in Pestalotiopsis fici

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Science china(Life Sciences) 2017年第6期60卷 656-664页

作者： Yanjing Zheng Xiuna Wang Xiaoling Zhang Wei Li Gang Liu Shihua Wang Xiufeng Yan Huixi zou Wen-Bing Yin zhejiang provincial(Wenzhou) Key Lab for Water Environment and Marine Biological Resources Protection College of Life and Environmental Science Wenzhou University State Key Laboratory of Mycology Institute of Microbiology Chinese Academy of Sciences School of Life Sciences Fujian Agriculture and Forestry University College of Life Sciences University of Chinese Academy of Sciences

The COP9 signalosome(CSN) is a highly conserved multiprotein complex in all eukaryotes and involved in regulation of organism development. In filamentous fungi, several lines of evidence indicate that fungal development and secondary metabolism(SM) are mediated by the fifth subunit of CSN, called CsnE. Here we uncover a connection with CsnE and conidial formation as well as SM regulation in the plant endophytic fungus Pestalotiopsis fici. A homology search of the P. fici genome with CsnE, involved in sexual development and SM in Aspergillus nidulans, identified PfCsnE. Deletion of PfcsnE resulted in a mutant that stopped conidial production, but the conidia are recovered in a PfcsnE complemented strain. This indicates that PfCsnE is required for the formation of conidia. Secondary metabolite analysis demonstrated that the ΔPfcsnE strain produced more chloroisosulochrin, less ficiolide A production in comparison to wild type(WT). Transcriptome analysis of WT andΔPfcsnE strains indicated that PfcsnE impacts the expression levels of 8.37% of 14,797 annotated genes. Specifically, nine biosynthetic gene clusters(BGCs) were up-regulated and three BGCs were down-regulated by PfCsnE. Our results suggest that PfCsnE plays major roles in SM regulation and conidial development in P. fici.

关键词： COP9 signalosome complex CSN secondary metabolism conidial development endophytic fungi

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杜鹃花属植物花香代谢研究进展

植物遗传资源学报 2024年第5期25卷 727-736页

作者：滕新蕾胡国伟邹荣娴陶楚冰孟羽肖政赵宏波浙江农林大学风景园林与建筑学院/浙江省园林植物种质创新与利用重点实验室/南方园林植物创新与利用国家林业和草原局重点实验室杭州311300 物产中大长乐林场有限公司杭州311300

花香是观赏植物重要的观赏性状,杜鹃花作为世界闻名的木本花卉,以花色丰富、花型多样闻名于世,其花香是评价杜鹃花品质的重要指标之一。研究表明萜烯类化合物、醇类、酯类、酮类化合物等是杜鹃花属植物花香化合物的主要成分,这些化合物... 详细信息

花香是观赏植物重要的观赏性状,杜鹃花作为世界闻名的木本花卉,以花色丰富、花型多样闻名于世,其花香是评价杜鹃花品质的重要指标之一。研究表明萜烯类化合物、醇类、酯类、酮类化合物等是杜鹃花属植物花香化合物的主要成分,这些化合物的释放受到花发育状态、释放部位以及环境条件的影响。萜烯类化合物是杜鹃花属植物最主要的花香成分,萜类合成酶基因是杜鹃花花香物质代谢途径中的主要调控基因,利用基因组结合代谢组学研究发现马银花TPS家族基因远多于其他无香型杜鹃花属植物。深入研究杜鹃花不同种和品种特征花香成分及其生物合成途径,对杜鹃花的芳香育种和综合利用有重要意义。本文论述了杜鹃花属植物香气成分的测定与分析方法、不同亚属杜鹃花资源的香气成分、香气合成释放规律以及香气物质生物合成途径和关键基因,为开展杜鹃花主要花香物质合成代谢的遗传规律研究和芳香品种选育提供参考。

关键词：杜鹃花花香成分生物合成萜烯类化合物

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不同杜鹃花品种对杜鹃冠网蝽的抗虫性评价

北方园艺 2024年第19期 62-72页

作者：陶楚冰胡国伟邹荣娴滕新蕾肖政赵宏波浙江农林大学风景园林与建筑学院浙江省园林植物品种创新与利用重点实验室南方园林植物创新与利用国家林业和草原局重点实验室浙江杭州311300 物产中大长乐林场有限公司浙江杭州311300

以不同类型杜鹃花品种为试材,采用田间调查法、相关性分析、系统聚类分析以及主成分分析综合评价不同杜鹃花品种对杜鹃冠网蝽的抗虫性差异,以期为杜鹃花抗虫品种的选育提参考依据。结果表明:不同杜鹃花品种对杜鹃冠网蝽(Stephanitis pyr... 详细信息

以不同类型杜鹃花品种为试材,采用田间调查法、相关性分析、系统聚类分析以及主成分分析综合评价不同杜鹃花品种对杜鹃冠网蝽的抗虫性差异,以期为杜鹃花抗虫品种的选育提参考依据。结果表明:不同杜鹃花品种对杜鹃冠网蝽(Stephanitis pyriodes)的抗虫性存在显著差异,杜鹃花抗虫性与虫害指数、叶片受害率、虫口数、叶绿素变化量负相关;而与栅栏组织厚度、栅栏组织与海绵组织比值、可溶性糖含量、可溶性蛋白质含量正相关。基于综合评价数据进行聚类分析,将供试材料分为5类,映山红、‘琉球红’和‘喜鹊登枝’为高抗品种;‘小青莲’‘兰花雨’‘紫萼’‘杨贵妃’‘康乃馨’为中抗品种;‘明朝’‘紫蝴蝶’‘王妃’‘红樱桃’‘沙浪’‘纺绸’‘立山之舞’‘御代之荣’‘天章’为低抗品种;‘白印’‘红苹果’‘粉白大叶’‘埃尔西李’为低感品种;‘红珍珠’‘凤冠’‘粉红泡泡’‘胭脂蜜’‘晚霞’为高感品种。

关键词：杜鹃花杜鹃冠网蝽抗虫性生理性状

季节变化对珠江口棘头梅童鱼肠道和水体中细菌群落结构的影响

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南方水产科学 2024年第4期20卷 77-87页

作者：郑秋实刘永李纯厚吴鹏肖雅元林琳刘玉邹剑浙江海洋大学水产学院浙江舟山316022 中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室/广东珠江口生态系统野外科学观测研究站/广东省渔业生态环境重点实验室广东广州510300

棘头梅童鱼(Collichthys lucidus)是珠江口常见的优势种和经济鱼类物种。为揭示棘头梅童鱼的生物生态学特征,利用16S rRNA基因的高通量测序技术,探究了季节变化对珠江口棘头梅童鱼肠道和水体中细菌群落结构的影响。结果表明,鱼类肠道细... 详细信息

棘头梅童鱼(Collichthys lucidus)是珠江口常见的优势种和经济鱼类物种。为揭示棘头梅童鱼的生物生态学特征,利用16S rRNA基因的高通量测序技术,探究了季节变化对珠江口棘头梅童鱼肠道和水体中细菌群落结构的影响。结果表明,鱼类肠道细菌以变形菌门居多(78.89%),其次为厚壁菌门(6.70%)和拟杆菌门(5.45%);水体细菌以变形菌门(40.32%)和拟杆菌门(37.97%)为主。棘头梅童鱼肠道中的优势菌属存在明显季节变化,春、夏季鱼类肠道中优势细菌为嗜冷杆菌(Psychrobacter),秋季为发光杆菌(Photobacterium),而冬季为假单胞菌(Pseudomonas)、Sva0996_marine_group、弧菌(Vibrio)和甲基杆菌(Methylobacterium)。冬季棘头梅童鱼肠道菌群的α多样性指数最高,秋季最低。春季和夏季鱼类肠道细菌组成无显著性差异(p>0.05),而其余季节间存在显著性差异(p0.4,p<0.01)。细菌共现网络分析显示冬季鱼类肠道和水环境的共有菌群比例最低,而夏季最高。研究表明,季节变化对珠江口棘头梅童鱼的肠道菌群和水体细菌群落组成均存在影响。

关键词：棘头梅童鱼肠道菌群共现网络 16S rRNA基因珠江口