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Effects of Ca^(2+) channel blockers on store-operated Ca^(2+) channel currents of Kupffer cells after hepatic ischemia/reperfusion injury in rats

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World Journal of Gastroenterology 2006年第29期12卷 4694-4698页

作者： Nan Jiang zong-ming Zhang Liang Liu Chi Zhang Yan-Lu Zhang Zi-Chao Zhang Department of General Surgery Digestive Medical Center First Affiliated Hospital of TsinghuaUniversity Beijing 100016 China Department of 1st General Surgery of Tongji Hospital Tongji University Shanghai 200065 China

AIM： To study the effects of hepatic ischemia/ reperfusion （I/R） injury on store-operated calcium channel （SOC） currents （Isoc） in freshly isolated rat Kupffer cells, and the effects of Ca^2＋ channel blockers, 2-aminoethoxydiphenyl borate （2-APB）, SK＆F96365, econazole and miconazole, on Isoc in isolated rat Kupffer cells after hepatic I/R injury.METHODS： The model of rat hepatic I/R injury was established. Whole-cell patch-clamp techniques were performed to investigate the effects of 2-APB, SK＆F96365, econazole and miconazole on Isoc in isolated rat Kupffer cells after hepatic I/R injury.RESULTS： I/R injury significantly increased Isoc from -80.4±25.2pA to -159.5±34.5pA （^bp 〈 0.01, n = 30）. 2-APB （20, 40, 60, 80, 100 pmol/L）, SK＆F96365 （5, 10, 20, 40, 50 pmol/L）, econazole （0.1, 0.3, 1, 3, 10 μmol/L） and miconazole （0.1, 0.3, 1, 3, 10 μmol/L） inhibited Isoc in a concentration-dependent manner with IC50 of 37.41 μmol/L （n = 8）, 5.89 μmol/L （n = 11）, 0.21 μmol/L （n = 13）, and 0.28 μmol/L （n = 10）. The peak value of Isoc in the I-V relationship was decreased by the blockers in different concentrations, but the reverse potential of Isoc was not transformed. CONCLUSION： SOC is the main channel for the influx of Ca^2＋ during hepatic I/R injuries. Calcium channel blockers, 2-APB, SK＆F96365, econazole and miconazole,have obviously protective effects on I/R injury, probably by inhibiting Isoc in Kupffer cells and preventing the activation of Kupffer cells.

关键词： Kupffer cell Ischemia/reperfusion Storeoperated calcium channel currents 2-aminoethoxydiphenylborate SK&F96365 Econazole Miconazole

Unprecedented α-Octamolybdate Derivative Containing Hexad-track Cu―bpe Chains,2{[Cu(bpe)]_6[α-Mo_8O_(26)]_(3/2)}·[Cu(bpe)]_3·3H_2O

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Chemical Research in Chinese Universities 2011年第2期27卷 166-169页

作者： SHA Jing-quan LI Xin PENG Jun zong Xi-ming ZHANG Yu-hong YAN Hong Heilongjiang Provincial Key Laboratory of Biomaterials and Biological Medicine Formulation School of Pharmacy Jiamusi University Jiamusi 154007 P. R. China Key Laboratory of Polyoxometalate Science Ministry of Education Faculty of Chemistry Northeast Normal University Changchun 130024 P. R. China

A new inorganic-organic hybrid constructed from α-octamolybdate polyoxometalates modified by hexad- track Cu―bpe chains, 2{[Cu（bpe）]6[α-Mo8O26]3/2}·[Cu（bpe）]3·3H2O[1, bpe=bis（4-pyridyl）ethylene], has been synthesized and structurally characterized. X-Ray diffraction analysis reveals that each α-octamolybdate cluster links six transition metal complex chains and the title compound has represented the highest connective number of M―N chains modified octamolybdate POMs to date. Note that there are two crystallographically unique [α-Mo8O26]n- clusters and eight unique copper sites, which play an important role in the overall architecture of hybrid compounds. Additionally, the electrochemical properties of the title compound were studied.

关键词： Octamolybdate Hydrothermal synthesis Multi-track chain Electrochemical property

C^3:Consensus Cancer Driver Gene Caller

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Genomics, Proteomics & Bioinformatics 2019年第3期17卷 311-318页

作者： Chen-Yu Zhu Chi Zhou Yun-Qin Chen Ai-zong Shen zong-ming Guo Zhao-Yi Yang Xiang-Yun Ye Shen Qu Jia Wei Qi Liu Department of Endocrinology&Metabolism Shanghai Tenth People’s HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China Department of Ophthalmology Ninghai First HospitalNinghai 315600China R&D Information Innovation Center ChinaAstraZenecaShanghai 201203China Shanghai Chest Hospital Shanghai Jiaotong UniversityShanghai 200240China Department of Pharmacy The First Affiliated Hospital of University of Science and Technology of ChinaHefei 230036China

Next-generation sequencing has allowed identification of millions of somatic mutations in human cancer cells.A key challenge in interpreting cancer genomes is to distinguish drivers of cancer development among available genetic *** address this issue,we present the first webbased application,consensus cancer driver gene caller(C^3),to identify the consensus driver genes using six different complementary strategies,i.e.,frequency-based,machine learning-based,functional bias-based,clustering-based,statistics model-based,and network-based *** application allows users to specify customized operations when calling driver genes,and provides solid statistical evaluations and interpretable visualizations on the integration results.C^3 is implemented in Python and is freely available for public use at http://***/c3.

关键词： Somatic mutation Cancer driver genes Consensus Data integration Web server

Using CropSyst to Simulate Spring Wheat Growth in Black Soil Zone of Northeast China

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Pedosphere 2006年第3期16卷 354-361页

作者： WANG zong-ming, ZHANG Bai LI Xiao-Yan SONG Kai-Shan LIU Dian-Wei ZHANG Shu-Qing Northeast Institute of Geography and Agricultural Ecology Chinese Academy of Sciences Changchun 130012 （China）

Available water and fertilizer have been the main limiting factors for yields of spring wheat, which occupies a large area of the black soil zone in northeast China; thus, the need to set up appropriate models for scenario analysis of cropping system models has been increasing. The capability of CropSyst, a cropping system simulation model, to simulate spring wheat growth of a widely grown spring cultivar, 'Longmai 19', in the black soil zone in northeast China under different water and nitrogen regimes was evaluated. Field data collected from a rotation experiment of three growing seasons (1992-1994) were used to calibrate and validate the model. The model was run for 3 years by providing initial conditions at the beginning of the rotation without reinitializing the model in later years in the rotation sequence. Crop input parameters were set based on measured data or taken from CropSyst manual. A few cultivar-specific parameters were adjusted within a reasonable range of fluctuation. The results demonstrated the robustness of CropSyst for simulating evapotranspiration, aboveground biomass, and grain yield of 'Longmai 19' spring wheat with the root mean square errors being 7%, 13% and 13% of the observed means for evapotranspiration (ET), grain yield and aboveground biomass, respectively. Although CropSyst was able to simulate spring production reasonably well, further evaluation and improvement of the model with a more detailed field database was desirable for agricultural systems in northeast China.

关键词： black soil zone of northeast China cropping system simulation model validation spring wheat

The Putative Ser/Thr Protein Kinase Gene GmAAPKfrom Soybean is Regulated by Abiotic Stress

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Journal of Integrative Plant Biology 2006年第3期48卷 327-333页

作者： Guang-Zuo Luo Yong-Jun Wang Jin-Song Zhang zong-ming Xie Jun-Yi Gai Shou-Yi Chen State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology the Chinese Academy of Sciences Beijing 100101 China National Center for Soybean Improvement Nanjing Agricultural University Nanjing 210095 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China

Osmotic stress caused by dehydration or high-salt conditions poses a major environmental challenge for plant growth and development. Many studies have shown that protein kinases are important regulators during osmotic stress in plants. In the present study, we describe GmAAPK, a soybean （Glycine max （L.） Merr.） gene coding for a putative serine/threonine protein kinase （abscisic acid （ABA）-activated protein kinase （AAPK））. The cDNA for GmAAPKis 1 409 bp long and contains a single long open reading frame representing a complete coding region of 359 amino acids. GmAAPKwas mapped onto the D1a＋Q linkage group. Its transcripts are expressed in all tissues, but at high levels in the cotyledon. GmAAPKmRNA was induced by polyethylene glycol, ABA, Ca^2＋ and Na^＋, but not cold （4℃） treatments in soybean leaves. The results suggest that GmAAPK may participate in the regulatory process during osmotic stresses in soybean.

关键词： abiotic stress protein kinase SnRK2 soybean.

Therapeutic options for intermediate-advanced hepatocellular carcinoma

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World Journal of Gastroenterology 2011年第13期17卷 1685-1689页

作者： zong-ming Zhang Jin-Xing Guo Zi-Chao Zhang Nan ]iang Zhen-Ya Zhang Li-Jie Pan Department of General Surgery Digestive Medical Center The First Affiliated Hospital Schoolof Medicine Tsinghua University Beijing 100016 China

Hepatocellular carcinoma (HCC) is one of the most common malignancies, ranking the sixth in the world, with 55% of cases occurring in China. Usually, patients with HCC did not present until the late stage of the disease, thus limiting their therapeutic options. Although surgical resection is a potentially curative modality for HCC, most patients with intermediate-advanced HCC are not suitable candidates. The current therapeutic modalities for intermediate-advanced HCC include: (1) surgical procedures, such as radical resection, palliative resection, intraoperative radiofrequency ablation or cryosurgical ablation, intraoperative hepatic artery and portal vein chemotherapeutic pump placement, two-stage hepatectomy and liver transplantation; (2) interventional treatment, such as transcatheter arterial chemoembolization, portal vein embolization and image-guided locoregional therapies; and (3) molecularly targeted therapies. So far, how to choose the therapeutic modalities remains controversial. Surgeons are faced with the challenge of providing the most appropriate treatment for patients with intermediate-advanced HCC. This review focuses on the optional therapeutic modalities for intermediateadvanced HCC.

关键词： Hepatocellular carcinoma Intermediateadvanced Surgical procedure Interventional treatment Molecularly targeted therapy

Diagnostic value and safety of medical thoracoscopy for pleural effusion of different causes

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World Journal of Clinical Cases 2022年第10期10卷 3088-3100页

作者： Xiao-Ting Liu Xi-Lin Dong Yu Zhang Ping Fang Hong-Yang Shi zong-Juan ming Department of Respiratory Medicine The Second Affiliated Hospital of Xi'an Jiaotong UniversityXi'an 710004Shaanxi ProvinceChina Department of Respiratory The Second Affiliated Hospital of Xi'an Jiaotong UniversityXi'an 710004Shaanxi ProvinceChina

BACKGROUND Pleural effusions occur for various reasons,and their diagnosis remains challenging despite the availability of different diagnostic *** thoracoscopy(MT)can be used for both diagnostic and therapeutic purposes,especially in patients with undiagnosed pleural *** To assess the diagnostic efficacy and safety of MT in patients with pleural effusion of different *** Between January 1,2012 and April 30,2021,patients with pleural effusion underwent MT in the Department of Respiratory Medicine,The Second Affiliated Hospital of Xi’an Jiaotong University(Shaanxi,China).According to the discharge diagnosis,patients were divided into malignant pleural effusion(MPE),tuberculous pleural effusion(TBPE),and inflammatory pleural effusion(IPE)*** information,and tuberculosis-and effusion-related indices of the three groups were *** diagnostic yield,diagnostic accuracy,performance under thoracoscopy,and complications of patients were compared among the three ***,the significant predictive factors for diagnosis between the MPE and TBPE groups were *** Of the 106 patients enrolled in this 10-year study,67 were male and 39 female,with mean age of 57.1±14.184 *** the 74 thoracoscopy-confirmed patients,41(38.7%)had MPE,21 had(19.8%)TBPE,and 32(30.2%)were *** diagnostic yield of MT was 69.8%(MPE:75.9%,TBPE:48.8%,and IPE:75.0%,with diagnostic accuracies of 100%,87.5%,and 75.0%,respectively).Under thoracoscopy,single or multiple pleural nodules were observed in 81.1%and pleural adhesions in 34.0%with pleural *** most common complication was chest pain(41.5%),followed by chest tightness(11.3%)and fever(10.4%).Multivariate logistic regression analyses showed effusion appearance[odds ratio(OR):0.001,95%CI:0.000-0.204;P=0.010]and carcinoembryonic antigen(OR:0.243,95%CI:0.081-0.728;P=0.011)as significant for differentiating MPE and TBPE,with area under the receiver operating cha

关键词： Medical thoracoscopy Pleural effusion Diagnostic value Safety Thoracoscopic performance Differential diagnosis

液相色谱串联质谱法测定青少年抑郁症患者血清和尿液中色氨酸代谢物的水平

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临床检验杂志 2024年第8期42卷 561-566页

作者：马萌萌滕晓明陆柳叶致含宗明同济大学附属妇产科医院生殖医学中心上海201204 同济大学附属东方医院检验科上海200120

目的观察并比较液相色谱串联质谱技术检测青少年抑郁症患者血清和尿液中色氨酸-犬尿氨酸(TRP-KP)通路代谢物的浓度。方法对该通路中主要代谢物如色氨酸(TRP)、犬尿氨酸(KYN)、犬尿酸(KYNA)和3-羟基犬尿氨酸(3-HK)采用同位素标记法进行... 详细信息

目的观察并比较液相色谱串联质谱技术检测青少年抑郁症患者血清和尿液中色氨酸-犬尿氨酸(TRP-KP)通路代谢物的浓度。方法对该通路中主要代谢物如色氨酸(TRP)、犬尿氨酸(KYN)、犬尿酸(KYNA)和3-羟基犬尿氨酸(3-HK)采用同位素标记法进行定量分析。使用岛津C18色谱柱,0.2%甲酸水溶液和乙腈进行梯度洗脱分离,7 min内采用正离子和多反应监测模式进行检测。考察线性范围、精密度等参数。用该方法检测143例青少年抑郁症患者和110例体检健康者血液和尿液样本并比较。结果以TRP为例,血清和尿液的线性范围分别为0.54~107.84μmol/L和0.74~147.06μmol/L。批内变异系数(CV)≤6.3%,批间CV≤3.22%,实验室总CV≤6.5%。抑郁组和对照组血清和尿液检测结果表明,抑郁组KYNA、KYN和TRP水平低于对照组,而3-HK水平高于对照组,差异具有统计学意义(P<0.01)。除TRP外,其他代谢物水平在尿液中显著高于血清中,差异具有统计学意义(P<0.01)。结论与血清相比,尿液中TRP-KP的代谢物即KYN、KYNA和3-HK浓度更高,由于尿液收集过程是无创的,因此检测尿液中TRP-KP代谢物具有更大的优势。

关键词：色氨酸-犬尿氨酸通路 3-羟基犬尿氨酸抑郁症串联质谱

Photocatalytic oxidation of nitric oxide from simulated flue gas by wet scrubbing using ultraviolet/TiO_2/H_2O_2 process

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Journal of Central South University 2015年第1期22卷 82-87页

作者：张波仲兆平付宗明 Key Laboratory of Energy Thermal Conversion and Control of Ministry of Education(School of Energy and Environment Southeast University)

Nitric oxide(NO) from flue gas is hard to remove because of low solubility and reactivity. A new technology for photocatalytic oxidation of NO using ultraviolet(UV)/TiO2/H2O2 process is studied in an efficient laboratory-scale reactor. Effects of several key operational parameters on NO removal efficiency are studied, including TiO2 content, H2O2 initial concentration, UV lamp power, NO initial content, oxygen volume fraction and TiO2/H2O2 solution volume. The results illustrate that the NO removal efficiency increases with the increasing of H2O2 initial concentration or UV lamp power. Meanwhile, a lower NO initial content or a higher TiO2/H2O2 solution volume will result in higher NO removal efficiency. In addition, oxygen volume fraction has a little *** highest NO removal efficiency is achieved at the TiO2 content of 0.75 g/L, H2O2 initial concentration of 2.5 mol/L, UV lamp power of 36 W, NO initial content of 206×10-6 and TiO2/H2O2 solution volume of 600 m L. It is beneficial for the development and application of NO removal from coal-fired flue gas with UV/TiO2/H2O2 process.

关键词： photocatalytic oxidation nitric oxide UV/TiO2/H2O2 process