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Maize ABP2 enhances tolerance to drought and salt stress in transgenic Arabidopsis

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Journal of Integrative Agriculture 2018年第11期17卷 2379-2393页

作者： zong na LI Xing-juan WANG Lei WANG Ying WEN Hong-tao LI Ling ZHANG Xia FAN Yun-liu ZHAO Jun national Key Facility for Crop Gene Resources and Genetic Improvement/Faculty of Maize Functional Genomics Biotechnology Research Institute Chinese Academy of Agricultural Sciences Guangdong Key Laboratory of Biotechnology for Plant Development/College of Life Science South China Normal University

Abiotic stresses, especially drought and salt, severely affect maize production, which is one of the most important cereal crops in the world. Breeding stress-tolerant maize through biotechnology is urgently needed to maintain maize production. Therefore, it is important to identify new genes that can enhance both drought and salt stress tolerance for molecular breeding. In this study, we identified a maize ABA （abscisic acid）-responsive element （ABRE） binding protein from a 17-day post-pollination （dpp） maize embryo cDna library by yeast one-hybrid screen using the ABRE2 sequence of the maize Cat1 gene as bait. This protein, designated, ABRE binding protein 2 （ABP2）, belongs to the bZIP transcription factor family. Endogenous expression of ABP2 in maize can be detected in different tissues at various development stages, and can be induced by drought, salt, reactive oxygen species （ROS）-generating agents, and ABA treatment. Constitutive expression of ABP2 in transgenic Arabidopsis plants enhanced tolerance to drought and salt stress, and increased sensitivity to ABA. In exploring the mechanism by which ABP2 can stimulate abiotic stress tolerance, we found that ROS levels were reduced and expression of stress-responsive and carbon metabolism-related genes was enhanced by constitutive ABP2 expression in transgenic plants. In Short, we identified a maize bZIP transcription factor which can enhance both drought and salt tolerance of plants.

关键词： ABP2 maize transcription factor drought salt transgenic Arabidopsis

RoGFP1 is a quantitative biosensor in maize cells for cellular redox changes caused by environmental and endogenous stimuli

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RoGFP1 is a quantitative biosensor in maize cells for cellul...

Genome-wide association study and metabolic pathway prediction of barrenness in maize as a response to high planting density

第一届全国玉米生物学学术研讨会

作者： Xiaoning Liu Jiamei Wu Hao Liu na zong 赵军 Faculty of Maize Functional Genomics Department of Crop Genome Research and Genetic ImprovementBiotechnology Research InstituteChinese Academy of Agricultural Sciences

Reduction-oxidation-sensitive green fluorescent proteins(roGFPs)have been demonstrated to be valuable tools in sensing cellular redox changes in mammalian cells and model plants,yet have not been applied in crops such as *** we report the characteristics of roGFP1 in transiently transformed maize mesophyll protoplasts in response to environmental stimuli and knocked-down expression of

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Journal of Integrative Agriculture 2022年第12期21卷 3514-3523页

作者： ZHANG Xu-huan LIU Hao MA Xu-hui ZHOU Gu-yi RUAN Hong-qiang CUI Hong-wei PANG Jun-ling KHAN Ullah Siffat zong na WANG Ren-zhong LENG Peng-fei ZHAO Jun Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081P.R.China State Key Laboratory of Vegetation and Environmental Change Institute of BotanyChinese Academy of SciencesBeijing 100093P.R.China

Increasing the planting density is one way to enhance grain production in ***,high planting density brings about growth and developmental defects such as barrenness,which is the major factor limiting grain *** this study,the barrenness was characterized in an association panel comprising 280 inbred lines under normal(67500 plants ha–1,ND)and high(120000 plants ha–1,HD)planting densities in 2017 and *** population was genotyped using 776254 single nucleotide polymorphism(SNP)markers with criteria of minor allele frequency>5%andnalysis indicated that the metabolic pathways of amino acids,such as glutamate and arginine,and the mitogen-activated protein kinase(MAPK)signaling pathway might play important roles in tolerance to high planting *** results provide insights into the genetic basis of high planting density tolerance and will facilitate high yield maize breeding.

关键词： Zea mays L high planting density barrenness genome-wide association study MAPK pathway

Induction of nicotine in tobacco by herbivory and its relation to glucose oxidase activity in the labial gland of three noctuid caterpillars

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Chinese Science Bulletin 2004年第15期49卷 1596-1601页

作者： zong na1,2 & WANG Chenzhu1 1.State Key Laboratory of Integrated Management of Insects & Rodents,Institute of Zoology,Chinese Academy of Sciences,Beijing 100080,China 2.Graduate School of Chinese Academy of Sciences,Beijing 100080,China Correspondence should be addressed to Wang Chenzhu(e-mail: czwang@***) State Key Laboratory of Integrated Management of Insects & Rodents Institute of Zoology Chinese Academy of Sciences Beijing China Graduate School of Chinese Academy of Sciences Beijing China

Tobacco Nicotiana tabacum L. is a host plant of Helicoverpa armigera (Hübner), Helicoverpa assulta Guenée and Spodoptera litura (Fabricius) (Lepidoptera, Noctuidae). The difference in leaf nicotine response to the feeding by these three larvae and the mechanical simulation of their feeding was examined by HPLC. Results indicated that nico- tine induction was suppressed by H. armigera and H. assulta larvae feeding or by simulated damage treated with their labial glands extracts. The production of nicotine was also suppressed by the glucose oxidase from Aspergillus niger when it was treated on mechanically wounded leaf area. On the contrary, the nicotine production was stimulated by S. litura larva feeding or by simulated damage treated with its labial gland extract. Heat denature can not counteract the stimulation effect of the S. litura labial gland extracts to to- bacco nicotine production. The glucose oxidase activity was detected in labial gland extracts of both H. armigera and H. assulta, but the activity in H. armigera was significantly higher than that in H. assulta. No glucose oxidase activity was detected in labial gland extracts of S. litura. It is shown that the glucose oxidase activity in labial glands of caterpil- lars plays an important role in the nicotine response to her- bivory. The glucose oxidase was mainly contained in the la- bial gland of H. armigera larva, and had the highest activity at pH 7.0. D-Glucose was the optimal substrate of the glucose oxidase. Labial gland glucose oxidase activities varied daily during larval development with high activities found when larvae were actively feeding.

关键词：烟碱烟草葡萄糖氧化酶三夜蛾毛虫棉铃虫烟毒虫斜纹夜蛾

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人才在饲料企业发展中的影响因素分析

中国饲料 2021年第6期 147-150页

作者：张淼宗娜河北地质大学华信学院河北石家庄050700

人才是第一生产力,随着各地人才引进政策的相继出台,人才在社会上的地位逐步提升,也从另一方面反映出人才的重要作用。作为传统行业,饲料企业要想取得更加长远的发展,就要加强企业人才引进。本文从人才的影响因素入手进行分析,从内部环... 详细信息

人才是第一生产力,随着各地人才引进政策的相继出台,人才在社会上的地位逐步提升,也从另一方面反映出人才的重要作用。作为传统行业,饲料企业要想取得更加长远的发展,就要加强企业人才引进。本文从人才的影响因素入手进行分析,从内部环境、外部政策、整体水平3个方面分析了目前人才在饲料企业中的发展现状,指出了目前在人才政策、人才环境、管理层意识及技术资金投入方面的不足,提出了加强饲料企业人才政策建设、创优饲料企业内部人才发展环境、提升管理层人才引进意识以及加大相关技术资金投入等措施,旨在使人才在饲料企业内部有更好的发展,也能提升饲料企业的竞争力及发展潜力。

关键词：人才饲料企业影响因素人才引进

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中蒙刑事被害人权利保护比较研究

内蒙古大学学报（哲学社会科学版） 2008年第4期40卷 17-21页

作者：宗那生麻俐内蒙古大学法学院内蒙古呼和浩特010021

中国和蒙古国对被害人的保护在刑事实体法和刑事程序法方面都有很大差异,应当借鉴蒙古国先进的立法经验,从实体法和程序法角度确立被害人的知情权和上诉权,并进一步完善被害人的自诉权,从而构建我国的被害人权利保护机制。

关键词：刑事诉讼被害人权利保护

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天然多糖及衍生物在金属缓蚀剂中的研究进展

表面技术 2021年第2期50卷 221-231页

作者：郑云香王向鹏宗丽娜中国石油大学胜利学院化学工程学院山东东营257061

缓蚀剂是一种减缓金属在外界环境中发生腐蚀反应的有效物质。随着人类绿色环保意识的增强,来源广、可再生、易降解、含有大量活性吸附中心的天然大分子链多糖物质已成为目前环境友好型缓蚀剂的研究热点之一。概述了近几年国内外天然多... 详细信息

缓蚀剂是一种减缓金属在外界环境中发生腐蚀反应的有效物质。随着人类绿色环保意识的增强,来源广、可再生、易降解、含有大量活性吸附中心的天然大分子链多糖物质已成为目前环境友好型缓蚀剂的研究热点之一。概述了近几年国内外天然多糖及衍生物和复合材料在抑制金属腐蚀方面的研究,重点综述了淀粉、纤维素、壳聚糖和其他天然多糖类物质的分子结构特点及在不同腐蚀环境中对金属的缓蚀性能。在此基础上,梳理了各类天然多糖物质的化学改性合成方法及改性后各衍生物的缓蚀能力,简要介绍了部分基于多糖物质或其衍生物制备的复合材料在缓蚀领域的研究进展。同时,从吸附模型及吸附方式(物理吸附、化学吸附)等方面,归纳了多糖类物质保护金属的作用机制。最后,分析了各类多糖及衍生物在金属缓蚀方面所存在的几点不足,如单一未改性的天然物质缓蚀效果差,部分改性产品溶解性差,部分改性方法复杂,缓蚀机理研究较少等,并展望了各类多糖及衍生物在未来缓蚀剂领域的发展趋势。

关键词：缓蚀剂天然多糖淀粉纤维素壳聚糖衍生物缓蚀机制缓蚀性能

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玉米生长条件对玉米原生质体转化的影响研究

中国农业科技导报 2014年第5期16卷 147-153页

作者：谢礼宗娜赵军中国农业科学院生物技术研究所作物基因组与遗传改良研究室玉米功能基因组创新团队农作物基因资源与基因改良国家重大科学工程北京100081

植物原生质体瞬时转化体系是一种高效、快捷的基因表达分析工具。为研究玉米生长条件对玉米原生质体活力和转化效率的影响,以玉米自交系Q319幼苗为研究对象,利用正交试验L9（3^4）探讨了玉米的生长条件（光照强度、光照周期、生长温度... 详细信息

植物原生质体瞬时转化体系是一种高效、快捷的基因表达分析工具。为研究玉米生长条件对玉米原生质体活力和转化效率的影响,以玉米自交系Q319幼苗为研究对象,利用正交试验L9（3^4）探讨了玉米的生长条件（光照强度、光照周期、生长温度和龄期）对玉米叶肉细胞原生质体转化的影响。实验结果显示上述四个因素都能够影响玉米叶肉细胞原生质体活力与转化效率,而光照强度的影响最大;进一步研究发现光照强度与玉米叶肉细胞原生质体的活力及转化效率呈负相关关系,玉米黄化苗原生质体转化效果最好,其原生质体活力及转化效率分别达到了100%和85%。另外,龄期为13 d的玉米黄化苗原生质体转化效率最高,龄期过小或过大,黄化苗的原生质体转化效率都会下降。

关键词：玉米Q319 原生质体转化生长条件转化效率

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格构增强复合材料圆筒的侧向抗冲击性能研究

玻璃钢／复合材料 2019年第11期 45-49,57页

作者：宗莉娜方海周辉江苏城市职业学院建筑工程学院南京210036 南京工业大学土木工程学院南京211816

本文采用试验的方法对格构增强复合材料桥梁防撞装置的侧向抗冲击性能进行研究。研究横向格构间距对复合材料圆筒体冲击极限荷载、冲击最大位移、耗能性能的影响,并根据试验数据进行数值拟合,得出格构间距对极限荷载的拟合曲线。试验中... 详细信息

本文采用试验的方法对格构增强复合材料桥梁防撞装置的侧向抗冲击性能进行研究。研究横向格构间距对复合材料圆筒体冲击极限荷载、冲击最大位移、耗能性能的影响,并根据试验数据进行数值拟合,得出格构间距对极限荷载的拟合曲线。试验中横向格构越密,极限压缩荷载或极限冲击荷载越大。

关键词：格构增强圆筒侧向冲击最大位移耗能