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The dynamic arms race during the early invasion of woodland strawberry by Botrytis cinerea revealed by dual dense high-resolution RNA-seq analyses

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Horticulture Research 2023年第12期10卷 175-189页

作者： Yibo Bai Haibin Wang Kaikai Zhu zong-ming Cheng College of Horticulture Nanjing Agricultural UniversityNanjing 210095China Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China Ministry of AgricultureTropical Crops Genetic Resources InstituteChinese Academy of Tropical Agricultural SciencesHaikou 571101China Co-innovation Center for Sustainable Forestry in Southern China Nanjing Forestry UniversityNanjingJiangsu 210037China

Necrotrophic pathogens replicate massively upon colonizing plants,causing large-scale wilting and death of plant ***-standing both mechanisms of pathogen invasion and host response processes prior to symptom appearance and their key regulatory networks is therefore important for defense against pathogen ***,we investigated the mechanisms of interaction between woodland strawberry(Fragaria vesca)leaves and gray mold pathogen(Botrytis cinerea)at 14 infection time points during the first 12 hours of the infection period using a dense,high-resolution time series dual transcriptomic analysis,characterizing the arms race between strawberry *** and *** before the appearance of localized *** leaves rapidly initiated strong systemic defenses at the first sign of external stimulation and showed lower levels of transcriptomic change later in the infection *** the host plants,*** showed larger-scale transcriptomic changes that persisted throughout the infection *** gene co-expression network analysis identified highly correlated genes in 32 gene expression modules between *** and *** two-hybrid and bimolecular fluorescence complementation assays revealed that the disease response protein FvRLP2 from woodland strawberry interacted with the cell death inducing proteins BcXYG1 and BcPG3 from *** of FvRLP2 in both strawberry and Arabidopsis inhibited *** infection,confirming these genes’respective *** findings shed light on the arms race process by which *** invades host plants and strawberry to defend against pathogen infection.

关键词： straw fir invasion

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Inaugural editorial

Horticulture Research 2014年第1期1卷 281-282页

作者： zong-ming(Max)Cheng Professor College ofHorticulture NanjingAgriculturalUniversityNanjingChina Department of Plant Sciences University of TennesseeKnoxvilleTNUSA

Horticulture is one of the oldest agricultural practices,dating back to the beginning of human civilization when humans collected naturally grown fruits,brought them to their settlements and purposely cultivated *** today plays crucial roles in our ***,nuts and vegetables are important foods that supplement staples like rice and corn and provide many essential *** trees,shrubs and flowers are essential for beautifying our living ***-cut flowers are associated with public congratulatory events like business openings and personal celebratory occasions like weddings,anniversaries and ***,herbs and specialty local fruits are inseparable parts of cultures and religious ***,horticulture is being used in combatting nutritional imbalance,obesity and mental health issues,providing educational materials for teaching biological sciences,remediating environmental disorders,and conserving biodiversity and environmental integrity.

关键词： flowers religious fruits

Analytical model for straight hemming based on minimum energy method

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2011年第7期12卷 532-542页

作者： Qian WANG Xiang-huai DONG He-zong LI Hai-ming ZHANG National Engineering Research Center of Die & Mold CAD Shanghai Jiao Tong University Shanghai 200030 China

An analytical model for straight hemming was developed based on minimum energy method to study the effect of flanging die corner radius on hemming *** order to calculate plastic strain and strain energy more exactly,the neutral layer of specimen corner after hemming is assumed to be a half ellipse with its major semi-axis *** hardening rule is adopted to describe bending and reverse bending processes neglecting Bauschinger *** model takes into account the material property parameters in order to satisfy a wide application range of different *** profile,creepage/growing(roll-in/roll-out) and maximum equivalent strain are predicted,which are greatly influenced by the flanging die corner *** facilities were designed and hemming experiments were *** predicted results of the present analytical model were compared to experimental data as well as finite element(FE) simulation *** was confirmed that they are in good agreement,and the model can be used to evaluate whether the material used as an outer panel for hemming is appropriate and to optimize process parameters when the material used for hemming is changed.

关键词： Analytical model Minimum energy method Defects Large deformation Hemming

Theoretical study on the pressurization characteristics of disc-seal single screw pump used in high viscosity oily sludge conveying field

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Petroleum Science 2022年第3期19卷 1361-1370页

作者： Zeng-Li Wang Hou-Wei Shi Shu-Yu Wang zong-ming Wang Mu-ming Hao Jun Wang National Engineering Laboratory of Offshore Geophysical and Exploration Equipment China University of Petroleum(East China) College of New Energy China University of Petroleum(East China)

The disc-seal single screw pump(DSSP)used in the field of high viscosity oily sludge transport has a huge ***,there is no research on the pressurization characteristics of the DSSP at present,which makes its application *** view of this,the pressurization process mathematical model of the DSSP was established based on the geometric model of the *** using this model,the pressurization characteristics of DSSP and the influence of working parameters on the pressurization process were studied combined with the principle of back-flow *** results show that the instantaneous pressurization process could be realized mainly depending on the reflux pressurization from the outlet chamber to the pressurization chamber when the screw rotor rotating angle is located at-5°to+5°.The pressure in the pressurization chamber will increase with the increase of working parameters which include inlet pressure,outlet pressure,screw rotation velocity and dynamic viscosity of fluid medium in the area of flow-back *** screw rotation velocity and the viscosity of the conveying medium have significant effects on the peak pressure in the pressurization chamber,and the peak pressure in the pressurization chamber is proportional to the screw rotation velocity and the dynamic viscosity coefficient of the conveying *** proportional coefficient between the peak pressure and the screw rotation velocity is 6.29×10~*** proportional coefficient between the peak pressure and the dynamic viscosity of the conveying medium is 6.28×10~6.

关键词： Disc-seal single screw pump Oily sludge Pressurization characteristics Mathematical model Reflux pressurization

Prevention and forecasting of rock burst hazards in coal mines

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Mining Science and Technology 2009年第5期19卷 585-591页

作者： DOU Lin-ming LU Cai-ping MU zong-long GAO ming-shi State Key Laboratory for Coal Resource and Mine Safety China University of Mining ＆ Technology Xuzhou Jiangsu 221008 China

Rock bursts signify extreme behavior in coal mine strata and severely threaten the safety of the lives of miners, as well as the effectiveness and productivity of miners. In our study, an elastic-plastic-brittle model for the deformation and failure of coal/rock was established through theoretical analyses, laboratory experiments and field testing, simulation and other means, which perfectly predict sudden and delayed rock bursts. Based on electromagnetic emission (EME), acoustic emission (AE) and microseism (MS) effects in the process from deformation until impact rupture of coal-rock combination samples, a multi-parameter identification of premonitory technology was formed, largely depending on these three forms of emission. Thus a system of classification for forecasting rock bursts in space and time was established. We have presented the intensity weakening theory for rock bursts and a strong-soft-strong (3S) structural model for controlling the impact on rock surrounding roadways, with the objective of laying a theoretical foundation and establishing references for parameters for the weakening control of rock bursts. For the purpose of prevention, key technical parameters of directional hydraulic fracturing are revealed. Based on these results, as well as those from deep-hole controlled blasting in coal seams and rock, integrated control techniques were established and anti-impact hydraulic props, suitable for roadways subject to hazards from rockbursts have also been developed. These technologies have been widely used in most coal mines in China, subject to these hazards and have achieved remarkable economic and social benefits.

关键词： rock bursts elastic-plastic-brittle model multi-parameter premonitory intensity weakening strong-soft-strong (3S) structure directional hydraulic fracturing anti-impact hydraulic prop

Auxin regulation and MdPIN expression during adventitious root initiation in apple cuttings

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Horticulture Research 2020年第1期7卷 955-966页

作者： Ling Guan Yingjun Li Kaihui Huang zong-ming(Max)Cheng College of Horticulture Nanjing Agricultural University210095 NanjingChina Institute of Pomology Jiangsu Academy of Agricultural SciencesJiangsu Key Laboratory for Horticultural Crop Genetic Improvement210014 NanjingChina Department of Plant Sciences University of TennesseeKnoxvilleTN 37831USA

Adventitious root(AR)formation is critical for the efficient propagation of elite horticultural and forestry *** decades of research,the cellular processes and molecular mechanisms underlying AR induction in woody plants remain *** examined the details of AR formation in apple(Malus domestica)M.9 rootstock,the most widely used dwarf rootstock for intensive production,and investigated the role of polar auxin transport in postembryonic *** formation begins with a series of founder cell divisions and elongation of the interfascicular cambium adjacent to vascular *** process is associated with a relatively high indole acetic acid(IAA)content and hydrolysis of starch *** auxin treatment promoted this cell division,as well as the proliferation and reorganization of the endoplasmic reticulum and Golgi *** contrast,treatment with the auxin transport inhibitor N-1-naphthylphthalamic acid(NPA)inhibited cell division in the basal region of the cuttings and resulted in abnormal cell divisions during the early stage of AR *** addition,PIN-FORMED(PIN)transcripts were differentially expressed throughout the whole AR development *** also detected upregulation of MdPIN8 and MdPIN10 during induction;upregulation of MdPIN4,MdPIN5,and MdPIN8 during extension;and upregulation of all MdPINs during AR *** research provides an improved understanding of the cellular and molecular underpinnings of the AR process in woody plants.

关键词： hydrolysis elite dwarf

Innovative Perspectives of Integrated Chinese Medicine on H. pylori

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Chinese Journal of Integrative Medicine 2018年第11期24卷 873-880页

作者： YE Hui SHI zong-ming CHEN Yao YU Jing ZHANG Xue-zhi Department of Traditional Chinese Medicine and Integrated Chinese Medicine Peking University First Hospital Department of Traditional Chinese Medicine Peking University International Hospital

Helicobacter pylori（H. pylori） treatment requires the development of more effective therapies, mainly owing to the challenges posed by the bacterial resistance to antibiotics. In China, critically high infection and antibiotic resistance rates have limited the application of classic H. pylori eradication therapies. Consequently, researchers are attempting to find new solutions by drawing from traditional medicine. This article reviews basic scientific and clinical progress in the use of integrated Chinese and Western medicine（IM） to treat H. pylori; describes the conflicting results between in vivo and in vitro studies in this regard; discusses the observed clinical effects of IM, with emphasis on traditional patent medicines; and proposes a role for IM in both the diagnosis and treatment of H. pylori, including the use of tongue manifestation as an early diagnostic method and capitalizing on IM＇s direct and indirect methods for enhancing antibiotic effect.

关键词： Helicobacter pylori integrated traditional Chinese and Western medicine phytomedicine

Expression level of miR-155 in peripheral blood

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Asian Pacific Journal of Tropical Medicine 2015年第3期8卷 214-219页

作者： Yu-Hui Zhang Liang-Hua Xia Jia-Mei Jin ming zong ming Chen Bo Zhang Department of Ultrasound East Hospital Affiliated to Medicine SchoolTongji University Department of Ultrasound Shanghai Huadong Hospital Affiliated to Medicine School.Fudan University

Objective:To investigate the relationship between the expression level of miR-155 and the severity of coronary lesion,and explore the action ***:Peripheral blood mononuclear cells(PBMC) were isolated form blood simple from patients with acute myocardial infarction(AMI),unstable angina(UAP).stable angina(SAP) and chesl pain syndrome(CPS).RT-PCR was performed to analysis the expression level of miR-155 in peripheral blood mononuclear cells,plasma and RAW264.7 *** was used to analyze the cell viability of OxLDL treated RAW264.7 ***:The expression level of miR-155 in blood sample from coronary heart disease patients was much lower than in the blood sample of non-coronary heart disease(P<0.05).The level of miR-155 in PBMCs was much higher in the blood sample from CPS group than the other three group,and the level of miR-155 in plasma was higher in the CPS group than in the UAP and the AMI group,the difference was statistically significant(P<0.05).The expression level of miR-155 in PBMCs is positively associated with the level in the plasma(r=0.861,P=0.000).OxLDL can induce the expression of miR-155 in RAW264.7 *** the cell viability of RAW264.7 *** with the concentration and the treatment time of OxLDL increased,the effort become more *** inhibition effort of OxLDL to RAW264.7macrophagocyte with high miR-155 expression is much lower than the control group,and it is statistically significant after treated for 12.24 and 48 ***:miR-155 plays a protective role in the progression of atherosclerosis,and it may be achieved by reducing the apoptosis effort of OxLDL to RAW264.7 macrophagocyte.

关键词： miR-155 Coronary heart disease RAW264.7 macrophagocyte

RESPONSES OF WETLAND ECO SECURITY TO LAND USE CHANGE IN WESTERN JILIN PROVINCE, CHINA

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Chinese Geographical Science 2005年第4期15卷 330-336页

作者： WANG Zhi-qiang ZHANG Bai YANG Guang WANG zong-ming ZHANG Shu-qing Northeast Institute of Geography and Agricultural Ecology Chinese Academy of Sciences Changchun 130012 P. R. China The Graduate School of Chinese Academy of Sciences Beijing 100039 P. R. China

By the use of the software of ARCGIS, dynamic changes of the landscape elements, landscape structure, conversion processes of the landscape gradients and the responses of wetland eco-security to land use/cover changes (LUCC) in the western Jilin Province were studied from 1930 to 2000. The results show that the landscape elements of grassland, wetland, forestland and water area shrank rapidly, and wetlands underwent huge losses in the study period due to the conversion from wetland into arable land and grassland in large quantities. The responses of wetland eco-security to LUCC were inverse evolvement of wetland vegetation, loss of biodiversity, water deficiency in wetland ecosystem, the changes of the heterogeneity of wetlands and the fragmentation of the wetland habitats. Suggestions were given for protection of wetlands and the regional sustainable development.

关键词： LUCC wetland eco-security RS GIS western Jilin Province