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Continuous Expression of Interferon Regulatory Factor 4 Sustains CD8^(+)T Cell Immunity against Tumor

Research 2024年第3期2023卷 433-444页

作者： Anze Yu Jinfei fu Zheng Yin Hui Yan xiang Xiao Dawei Zou Xiaolong Zhang Xiongbing Zu Xian C.Li Wenhao Chen Immunobiology and Transplant Science Center Department of SurgeryHouston Methodist Research Institute and Institute for Academic MedicineHouston Methodist HospitalHoustonTXUSA Department of Urology First Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdongChina Systems Medicine and Bioengineering Department Houston Methodist Neal Cancer CenterHoustonTXUSA Department of Radiology Houston Methodist HospitalWeill Cornell MedicineHoustonTXUSA Department of Urology Xiangya HospitalCentral South UniversityChangshaHunanChina Department of Surgery Weill Cornell MedicineCornell UniversityNew YorkNYUSA.

T-cell-based immunotherapy is gaining momentum in cancer treatment;however,our comprehension of the transcriptional regulation governing T cell antitumor activity remains *** objective of this study was to explore the function of interferon regulatory factor 4(IRF4)in antitumor CD8^(+)T cells using the TRAMP-C1 prostate cancer and B16F10 melanoma *** achieve this,we generated an Irf4^(GFP-DTR) mouse strain and discovered that CD8^(+)tumor-infiltrating lymphocytes(TILs)expressing high levels of *** exhibited a more differentiated PD-1high cell *** administering diphtheria toxin to tumor-bearing Irf4^(GFP-DTR) mice,we partially depleted ***^(+)TILs and observed an accelerated tumor *** specifically explore the function of IRF4 in antitumor CD8^(+)T cells,we conducted 3 adoptive cell therapy(ACT)***,depleting ***^(+)CD8^(+)TILs derived from ACT significantly accelerated tumor growth,emphasizing their crucial role in controlling tumor ***,deleting the Irf4 gene in antitumor CD8^(+)T cells used for ACT led to a reduction in the frequency and effector differentiation of CD8^(+)TILs,completely abolishing the antitumor effects of ***,we performed a temporal deletion of the Irf4 gene in antitumor CD8^(+)T cells during ACT,starting from 20 days after tumor implantation,which significantly compromised tumor ***,sustained expression of IRF4 is essential for maintaining CD8^(+)T cell immunity in the melanoma model,and these findings carry noteworthy implications for the advancement of more potent immunotherapies for solid tumors.

关键词： sustained immunity implantation

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Method for the extraction of circulating nucleic acids based on MOF reveals cell-free RNA signatures in liver cancer

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National Science Review 2024年第1期11卷 94-106页

作者： Yuqing Sun Haixin Yu Shaoqing Han Ruoxi Ran Ying Yang Yongling Tang Yuhao Wang Wenhao Zhang Heng Tang Boqiao fu Boshi fu Xiaocheng Weng Song-Mei Liu Hexiang Deng Shuang Peng xiang Zhou College of Chemistry and Molecular Sciences Key Laboratory of Biomedical Polymers-Ministry of Education Wuhan University Department of Digestive Surgical Oncology Union Hospital Tongji Medical College Huazhong University of Science and Technology Cancer Center Union HospitalTongji Medical CollegeHuazhong University of Science and Technology Department of Clinical Laboratory Center for Gene Diagnosis and Program of Clinical Laboratory Zhongnan Hospital of Wuhan University College of Chemistry and Materials Science Hubei Engineering University Department of Pharmacology School of Pharmacy China Medical University Taikang Center for Life and Medical Sciences Wuhan University Department of Hematology Zhongnan Hospital of Wuhan University

Cell-free RNA(cf RNA) allows assessment of health, status, and phenotype of a variety of human organs and is a potential biomarker to non-invasively diagnose numerous diseases. Nevertheless, there is a lack of highly efficient and bias-free cf RNA isolation technologies due to the low abundance and instability of cf ***, we developed a reproducible and high-efficiency isolation technology for different types of cell-free nucleic acids(containing cf RNA and viral RNA) in serum/plasma based on the inclusion of nucleic acids by metal-organic framework(MOF) materials, which greatly improved the isolation efficiency and was able to preserve RNA integrity compared with the most widely used research kit method. Importantly, the quality of cf RNA extracted by the MOF method is about 10-fold that of the kit method, and the MOF method isolates more than three times as many different RNA types as the kit method. The whole transcriptome mapping characteristics of cf RNA in serum from patients with liver cancer was described and a cf RNA signature with six cf RNAs was identified to diagnose liver cancer with high diagnostic efficiency(area under curve = 0.905 in the independent validation cohort) using this MOF method. Thus, this new MOF isolation technique will advance the field of liquid biopsy, with the potential to diagnose liver cancer.

关键词： MOF circulating cell-free RNA biomarker liver cancer diagnosis

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用低能离子溅射剥层和高能离子背散射组合方法进行薄膜原位分析

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物理学报 2000年第1期49卷 164-169页

作者：刘波姜蕾周筑颖贺勉鸿赵国庆宗祥福复旦大学现代物理研究所基于加速器的原子物理和原子核物理实验室上海200433 复旦大学材料科学系

介绍了将离子枪组合于卢瑟福背散射分析靶室中构成Ｓｐｕｔｔｅｒｉｎｇ／ＲＢＳ原位分析实验装置，用低能离子溅射剥层与高能离子背散射组合对薄膜样品进行成分和深埋层分析方法．给出了对样品分析的三个例子．对Ａｕ／Ｓｉ样品的分... 详细信息

介绍了将离子枪组合于卢瑟福背散射分析靶室中构成Ｓｐｕｔｔｅｒｉｎｇ／ＲＢＳ原位分析实验装置，用低能离子溅射剥层与高能离子背散射组合对薄膜样品进行成分和深埋层分析方法．给出了对样品分析的三个例子．对Ａｕ／Ｓｉ样品的分析着重讨论了Ａｕ在Ａｒ＋溅射剥层时的溅射速率；对Ｓｉ／ＧｅＳｉ／Ｓｉ和ＷＳｉｘ／ＳｉＯ２／Ｓｉ样品的深埋层分析，提高了样品分析的深度分辨率．

关键词：薄膜原位分析低能离子溅射剥层高能离子背散射

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活性铁含量对窖泥老化影响

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酿酒 2007年第4期34卷 83-85页

作者：向宗府湖南酒鬼酒股份有限公司湖南吉首416000

窖泥氧化还原电位(Eh值)决定着窖泥中铁存在形态。当窖泥处于中度还原状态时(Eh:0～200mv),活性铁含量最高,也最易出现老化现象;当窖泥处于强度还原状态时﹙Eh<0mv﹚,Fe2+转变为沉淀铁(FeS)老窖形成。通过人为控制条件,可促进窖泥微生... 详细信息

窖泥氧化还原电位(Eh值)决定着窖泥中铁存在形态。当窖泥处于中度还原状态时(Eh:0～200mv),活性铁含量最高,也最易出现老化现象;当窖泥处于强度还原状态时﹙Eh<0mv﹚,Fe2+转变为沉淀铁(FeS)老窖形成。通过人为控制条件,可促进窖泥微生物活动,加速新窖还原化进程,从而促成"百年老窖"形成。

关键词： Eh值活性铁沉淀铁老化窖泥百年老窖

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An efficient and safe strategy for germ cell-specific automatic excision of foreign DNA in F1 hybrid transgenic silkworms

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Insect Science 2024年第1期31卷 28-46页

作者： Dingpei Long Rongpeng Liu Yang Huang Anyao fu Yuli Zhang Zhanzhang Hao Qiang Li Hanfu Xu Zhonghuai xiang Aichun Zhao State Key Laboratory of Resource Insects Institute of Sericulture and Systems BiologySouthwest UniversityChongqingChina Department of Biology Georgia State UniversityAtlantaGeorgiaUSA Guangxi Institute of Sericulture Science NanningGuangxiChina Department of Mathematics and Statistics Georgia State UniversityAtlantaGeorgiaUSA

The safety of transgenic technology is a major obstacle in the popularization and use of transgenic silkworms and their *** sericulture,only the first filial generation(F1)hybrid eggs produced by cross-breeding Japanese and Chinese original strains are usually used for the large-scale breeding of silkworms,but this may result in uncontrolled transgene dispersal during the popularization and application of the F1 hybrid transgenic *** address this issue,we developed a safe and efficient strategy using the GAL4/Upstream activating sequence(UAS)system,the FLP/flippase recognition target(FRT)system,and the gonad-specific expression gene promoters(RSHP1p and Nanosp)for the germ cell-specific automatic excision of foreign DNA in the F1 hybrid transgenic *** established 2 types of activator strains,R1p::GAL4-Gr and Nsp::GAL4-Gr,containing the testis-specific GAL4 gene expression cassettes driven by RSHP1p or Nanosp,respectively,and 1 type of effector strain,UAS::FLP-Rg,containing the UAS-linked FLP gene expression *** FLP recombinase-mediated sperm-specific complete excision of FRT-flanked target DNA in the F1 double-transgenic silkworms resulting from the hybridization of R1p::GAL4-Gr and UAS::FLP-Rg was 100%,whereas the complete excision efficiency resulting from the hybridization of Nsp::GAL4-Gr and UAS::FLP-Rg ranged from 13.73%to 80.3%.Additionally,we identified a gene,sw11114,that is expressed in both testis and ovary of Bombyx mori,and can be used to establish novel gonad-specific expression systems in transgenic *** strategy has the potential to fundamentally solve the safety issue in the production of F1 transgenic silkworm eggs and provides an important reference for the safety of transgenic technology in other insect species.

关键词： Bombyx mori FLP/FRT system GAL4/UAS system germ cell-specific excision safety of transgenic technology transgenic silkworm

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Acceptor-Type Singlet Fission Material Based on Strong Absorption Tetracyanothienoquinoid Skeleton

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CCS Chemistry 2024年第9期6卷 2142-2149页

作者： Senhao Wang xiang-Yang Liu Mengfan Zhang Long Wang Ganglong Cui Hongbing fu Jiannian Yao Key Laboratory of Interface Science and Engineering in Advanced Materials Ministry of EducationTaiyuan University of TechnologyTaiyuan 030024 College of Chemistry and Material Science Sichuan Normal UniversityChengdu 610068 Key Laboratory of Theoretical and Computational Photochemistry Ministry of EducationCollege of ChemistryBeijing Normal UniversityBeijing 100875 Beijing Key Laboratory for Optical Materials and Photonic Devices Department of ChemistryCapital Normal UniversityBeijing 100048 Beijing National Laboratory for Molecules Science(BNLMS) Key Laboratory of PhotochemistryInstitute of ChemistryChinese Academy of SciencesBeijing 100190

The practical efficiency of singlet fission(SF)-based photovoltaic devices is still far from satisfactory due to the limited scope of SF materials suitable for device application and the scarcity of schemes available for triplet *** SF materials identi-fied to date are typically electron donors while acceptor-type SF materials remain largely ***,the combination of a conventional electron donor and SF-active electron acceptor could circumvent the competitive energy transfer channel and better play the unique advantages of the SF process,which might be an adequate alternative for practical *** this work,we presented a new acceptor-type SF material based on a tetracyanothienoquinoid *** a quinoid skeleton exhibited strong absorption,ultrafast SF process,and excellent *** transient spectroscopy and multireference calculations(XDWCASPT2),the SF dynamics were examined featuring the rapid generation and subsequent annihilation and/or partial dissociation of multiexciton ***,our results not only provide a robust acceptor-type SF material but also suggest an adequate donor–acceptor alternative for SF-based solar cells,which could pave the way for the practical application of such a potential process.

关键词： singlet fission acceptor quinoidal skeleton mutiexciton state multireference calculation transient spectroscopy

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Reduced macular thickness and vascular density in abnormal glucose metabolism patients:A meta-analysis of optical coherence tomography(OCT)and OCT angiography studies

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Chinese Medical Journal 2024年第9期137卷 1054-1068页

作者： xiangyu fu xiang Ren Wenyue Chen Danian Chen Department of Ophthalmology West China HospitalSichuan UniversityChengduSichuan 610041China Research Laboratory of Ophthalmology and Vision Sciences State Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengduSichuan 610041China Department of Ophthalmology The People’s Hospital of Guizhou ProvinceGuiyangGuizhou 550002China

Background:Alterations in macular thickness and vascular density before clinically visible diabetic retinopathy(DR)remain *** study aimed to determine whether retinal manifestations in abnormal glucose metabolism(AGM)patients differ from those in the healthy ***:PubMed,Embase,and Web of Science were searched between 2000 and *** eligibility criteria were AGM patients without *** and secondary outcomes measured by optical coherence tomography(OCT)and OCT angiography(OCTA)were analyzed and expressed as standardized mean differences(SMDs)with 95%confidence intervals(CIs).A random-effects model was used in the data *** potential publication bias for the variables was evaluated using Egger’s ***:A total of 86 observational studies involving 13,773 participants and 15,416 eyes were *** revealed that compared to healthy controls,the total macular thickness of AGM patients was thinner,including the thickness of fovea(-0.24,95%CI[-0.39,-0.08];P=0.002,I^(2)=87.7%),all regions of parafovea(-0.32,95%CI[-0.54,-0.11];P=0.003;I^(2)=71.7%)and the four quadrants of perifovea;the thickness of peripapillary retinal nerve fiber layer(pRNFL),macular retinal nerve fiber layer(mRNFL),and ganglion cell layer(GCL)also *** indicated that the superficial and deep vascular density decreased,the foveal avascular zone(FAZ)area enlarged,and the acircularity index(AI)reduced in AGM ***:Retinal thinning and microvascular lesions have occurred before the advent of clinically detectable DR;OCT and OCTA may have the potential to detect these preclinical ***:PROSPERO;http://***/prospero/;***42021269885.

关键词： Abnormal glucose metabolism Optical coherence tomography Optical coherence tomography angiography Diabetic retinopathy Prediabetes Meta-analysis

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Biological Evaluation of Lysionotin:a Novel Inhibitor of 5-Lipoxygenase for Anti-glioma

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Chinese Journal of Integrative Medicine 2024年第9期30卷 826-834页

作者： SHAO Xin-xin CHEN Cong LIU Jie LI Qing-jun HE Shan QI xiang-hua fu Xian-jun WANG Zhen-guo The First Clinical College of Medicine Shandong University of Traditional Chinese MedicineJinan250355China Institute for Literature and Culture of Chinese Medicine Shandong University of Traditional Chinese MedicineJinan250355China Key Laboratory of Classical Theory of Traditional Chinese Medicine Ministry of EducationShandong University of Traditional Chinese MedicineJinan250355China College of Pharmacy Shandong University of Traditional Chinese MedicineJinan250355China Experimental Center Shandong University of Traditional Chinese MedicineJinan250355China Neurology Department Affiliated Hospital of Shandong University of Traditional Chinese MedicineJinan250014China Marine Traditional Chinese Medicine Research Center Qingdao Academy of Traditional Chinese MedicineShandong University of Traditional Chinese MedicineQingdao266114Shandong ProvinceChina

Objective:To explore the potential mechanism of lysionotin in treating ***:First,target prediction based on Bernoulli Naïve Bayes profiling and pathway enrichment was used to predict the biological activity of *** binding between 5-lipoxygenase(5-LO)and lysionotin was detected by surface plasmon resonance(SPR)and molecular docking,and the inhibitory effects of lysionotin on 5-LO and proliferation of glioma were determined using enzyme inhibition assay in vitro and cell viability analysis,***,the pharmaceutical effect of lysionotin was explored by cell survival rate analysis and liquid chromatography with tandem mass spectrometry(LC-MS/MS).The protein expression,intracellular calcium ion concentration and cytoskeleton detection were revealed by Western blot,flow cytometry and fluorescence labeling,***:Target prediction and pathway enrichment revealed that lysionotin inhibited 5-LO,a key enzyme involved in the arachidonic acid metabolism pathway,to inhibit the proliferation of *** docking results demonstrated that 5-LO can be binding to lysionotin through hydrogen bonds,forming bonds with His600,Gln557,Asn554,and *** analysis further confirmed the interaction between 5-LO and ***,enzyme inhibition assay in vitro and cell survival rate analysis revealed that 50%inhibition concentration of lysionotin and the median effective concentration of lysionotin were 90 and 16.58µmol/L,respectively,and the results of LC-MS/MS showed that lysionotin inhibited the production of 5S-hydroperoxy-eicosatetraenoic acid(P<0.05),and moreover,the LC-MS/MS results indicated that lysionotin can enter glioma cells well(P<0.01)and inhibit their *** blot analysis demonstrated that lysionotin can inhibit the expression of 5-LO(P<0.05)and downstream leukotriene B4 receptor(P<0.01).In addition,the results showed that lysionotin affected intracellular calcium ion concentration by inh

关键词： 5-lipoxygenase inhibitor lysionotin 5-lipoxygenase leukotriene B4 calcium ion cytoskeleton

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Intermediate-mass black holes in Green pea galaxies(IMBH-GP)I:A candidate sample from LAMOST and SDSS

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Science China(Physics,Mechanics & Astronomy) 2024年第10期67卷 147-161页

作者： Ruqiu Lin Zhen-Ya Zheng Fang-Ting Yuan Jun-Xian Wang Chunyan Jiang Ning Jiang Lingzhi Wang Linhua Jiang xiang Ji Shuairu Zhu Xiaodan fu Key Laboratory for Research in Galaxies and Cosmology Shanghai Astronomical ObservatoryChinese Academy of SciencesShanghai 200030China School of Astronomy and Space Sciences University of Chinese Academy of SciencesBeijing 100049China CAS Key Laboratory for Research in Galaxies and Cosmology Department of AstronomyUniversity of Science and Technology of ChinaHefei 230026China Chinese Academy of Sciences South America Center for Astronomy(CASSACA) National Astronomical ObservatoriesChinese Academy of SciencesBeijing 100101China CAS Key Laboratory of Optical Astronomy National Astronomical ObservatoriesChinese Academy of SciencesBeijing 100101China Kavli Institute for Astronomy and Astrophysics Peking UniversityBeijing 100871China Department of Astronomy School of PhysicsPeking UniversityBeijing 100871China

The scaling relation of central massive black holes(M_(BH)s)and their host galaxies is well-studied for supermassive BHs(SM_(BH)s,M_(BH)≥10^(6)M_(⊙)).However,this relation has large uncertainties in the mass range of the intermediate-mass BHs(IM_(BH)s,M_(BH)~10^(3)-10^(6)M_(⊙)).Since Green pea(GP)galaxies are luminous compact dwarf galaxies,which may be likely to host less massive SM_(BH)s or even IM_(BH)s,we systematically search for M_(BH)s in a large sample of 2190 GP galaxies at z<0.4,selected from LAMOST and SDSS spectroscopic ***,we report a newly discovered sample of 59 M_(BH) candidates with broad Hα*** sample has a median stellar mass of 108.83±0.11M_(⊙)and hosts M_(BH)s with single-epoch virial masses ranging from M_(BH)~10^(4.7)to 10^(8.5)M_(⊙)(median 105.85±0.64M_(⊙)).Among the 59 M_(BH) candidates,36 have black hole masses M_(BH)≤10^(6)M_(⊙)(IM_(BH) candidates),one of which even has M_(BH)■10^(5)M_(⊙).We find that the M_(BH)-M_(*)relation of our M_(BH) sample is consistent with the M_(BH)-M_(bulge)relation for SM_(BH)s,while is above the M_(BH)-M_(*)relation for M_(BH)s in dwarf galaxies in the same mass ***,we show that 25 M_(BH) candidates,including 4 IM_(BH) candidates,have additional evidence of black hole activities,assessed through various methods such as the broad-line width,BPT diagram,mid-infrared color,X-ray luminosity,and radio *** studies show that it is very promising to find IM_(BH)s in GP galaxies,and the BH sample so obtained enables us to probe the connection between the M_(BH)s and compact dwarf galaxies in the low-redshift Universe.

关键词： SMBHs IMBHs Green pea galaxies compact dwarf galaxies

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亚微米IC器件中接触孔的填充和铝金属化工艺的技术

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Journal of Semiconductors 2000年第6期21卷 614-619页

作者：梁京黄榕旭郑国祥林健庞海舟宗祥福复旦大学材料科学系上海200433 上海先进半导体制造有限公司上海200233

在亚微米 IC器件的铝金属化工艺中 ,采用了阻挡层和硅化物后 ,发现随着铝淀积温度的升高 ,铝的阶梯覆盖率有所提高 .为克服高温淀积带来的问题 ,采用了两步的冷 /热铝溅射和浸润层等工艺代替原来的铝溅射 .通过研究和一系列的比较实验 ... 详细信息

在亚微米 IC器件的铝金属化工艺中 ,采用了阻挡层和硅化物后 ,发现随着铝淀积温度的升高 ,铝的阶梯覆盖率有所提高 .为克服高温淀积带来的问题 ,采用了两步的冷 /热铝溅射和浸润层等工艺代替原来的铝溅射 .通过研究和一系列的比较实验 ,发现影响铝填充性能的主要因素为 :热铝淀积温度、热铝淀积功率、Ti浸润层厚度及冷热铝厚度比 .由此得到了适合于实际亚微米

关键词：填充接触孔金属化工艺亚微米集成电路

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