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黄瓜-酸黄瓜异附加系鉴定、果实长度变异及其分子机制

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南京农业大学学报 2025年第1期48卷 108-120页

作者：刘紫薇冯贤波赵勤政夏磊娄群峰南京农业大学园艺学院/作物遗传与种质创新利用全国重点实验室江苏南京210095

[目的]本文旨在通过分析不同黄瓜-酸黄瓜异附加系的果实差异,探究附加系中导入酸黄瓜6号染色体引起黄瓜果实变短的分子机制,为黄瓜近缘野生种优异性状挖掘和利用提供理论依据。[方法]采用分子标记和Oligo-FISH鉴定黄瓜-酸黄瓜异附加系... 详细信息

[目的]本文旨在通过分析不同黄瓜-酸黄瓜异附加系的果实差异,探究附加系中导入酸黄瓜6号染色体引起黄瓜果实变短的分子机制,为黄瓜近缘野生种优异性状挖掘和利用提供理论依据。[方法]采用分子标记和Oligo-FISH鉴定黄瓜-酸黄瓜异附加系中的外源染色体,对栽培黄瓜‘北京截头'(CC3)和不同黄瓜-酸黄瓜异附加系的果实性状进行统计,开展石蜡切片和转录组测序分析,并利用实时荧光定量PCR技术(RT-qPCR)验证转录组测序结果。[结果]共鉴定出7种异附加系材料:附加酸黄瓜6号染色体CC-H06、8号染色体CC-H08、9号染色体CC-H09及10号染色体CC-H10的单体附加系;附加酸黄瓜6和10号染色体CC-H06+H10的双单体附加系;附加酸黄瓜6、7和11号染色体CC-H06+H07+H11以及附加酸黄瓜6、7、10、11和12号染色体CC-H06+H07+H10+H11+H12的多单体附加系。表型统计发现,CC-H08、CC-H09和CC-H10的子房和果实长度均与CC3相似,而CC-H06较CC3明显变短,表明酸黄瓜6号染色体导入会引起CC3果实变短。石蜡切片结果显示,CC-H06的果实细胞相对于CC3较大,但细胞数目却明显减少。转录组分析表明,CC-H06中分别有695和2 770个酸黄瓜及CC3来源的差异表达基因,主要富集在植物激素信号转导、玉米素生物合成、二萜生物合成、色氨酸代谢等激素合成通路。RT-qPCR 验证分析与转录组测定结果一致,说明结果真实可靠。[结论]酸黄瓜6号染色体导入会引起CC3果实变短和果实细胞数量减少,CC-H06的果实变短与生长素、细胞分裂素和赤霉素等植物激素共同调控有关。

关键词：黄瓜酸黄瓜异附加系(ALL) 果实转录组

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gPhone重力仪漂移改正方法研究

大地测量与地球动力学 2025年第1期45卷 104-110页

作者：王乐行刘子维江颖张晓彤李晖中国地震局地震研究所武汉市430071 武汉引力与固体潮国家野外科学观测研究站武汉市430071

对秭归站gPhone重力仪2017年全年数据进行预处理和各项改正后,利用4种方法进行漂移改正的对比研究,对多项式改正阶次的选取、各方法去漂移的效果评定、BEADS算法是否有效等问题进行计算分析。结果表明,用多项式拟合的方法去除gPhone重... 详细信息

对秭归站gPhone重力仪2017年全年数据进行预处理和各项改正后,利用4种方法进行漂移改正的对比研究,对多项式改正阶次的选取、各方法去漂移的效果评定、BEADS算法是否有效等问题进行计算分析。结果表明,用多项式拟合的方法去除gPhone重力仪数据漂移时,当阶数达到4阶、5阶左右便趋于稳定,对于一般研究推荐采用5阶多项式去漂移即可满足数据处理要求;用滤波法去漂移时需要关注边际效应,EEMD方法可操作性强,相较于小波滤波更具优势;BEADS算法能够实现趋势项拟合从而去除漂移的功能,但能否在各项研究中加以应用还需要进一步分析。该研究成果可为相关弹簧型重力仪漂移改正提供参考。

关键词： gPhone重力仪漂移改正多方法对比

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Flexible electrochemical fiber sensor for intracranial nitric oxide monitoring

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Science China Chemistry 2024年第8期67卷 2431-2433页

作者： ziwei liu Huisheng Peng State Key Laboratory of Molecular Engineering of Polymers Department of Macromolecular ScienceInstitute of Fiber Materials and Devicesand Laboratory of Advanced MaterialsFudan UniversityShanghai 200438China

Real-time and accurate monitoring of vital biomarkers in vivo forms a crucial foundation for health assessment,early intervention,and disease ***,continuous monitoring of intracranial nitric oxide(NO)levels plays a pivotal role in promptly assessing immune responses,neurovirulence,and cerebrovascular diseases[1].Differing from non-real-time monitoring methods like chemiluminescence[2]and fluorescent probes[3],implantable electrochemical sensors excel in simultaneously high temporal resolution,sensitivity,and cost-effectiveness[4].

关键词： prompt electrochemical monitoring

Studying the urban hierarchical pattern and spatial structure of China using a synthesized gravity model

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Science China Earth Sciences 2018年第12期61卷 1818-1831页

作者： Ruibo HAN Huhua CAO ziwei liu Department of Geographical Sciences University of Maryland College Park Department of Geography University of Ottawa

The Gravity Model(GM), one of the most classic models adopted by the domain of social sciences from physics, has been widely used to study the interactions between social identities. Previous research that has used the Gravity Model to study the interactions among cities have mostly used a single variable, such as population or GDP, to represent a city, which does not provide a comprehensive depiction of a city's influence. This paper develops a Synthesized Gravity Model(SGM) based on the traditional Gravity Model to study the evolution of the hierarchy of the Chinese urban system since the mid-1990 s. Under this model, socioeconomic variables are synthesized and are represented by the Influential Factor, while the Function Distance is derived from a Network Analysis that is based on multiple transportation methods. As an improvement on the GM, the SGM is used to accurately establish and represent the nodal structure of China's urban system, the evolution of its hierarchical structure,and the relationships that exist between the nodal structure and socioeconomic factors. The results based on the SGM indicate that China's national urban system is characterized by the emergence of urban clusters with stronger inter-city interactions since the 1990 s. However, the development among cities within certain urban clusters is not even, although the general pattern indicates a lessening inequality amongst cities. Spatially, while most cities at the top of the hierarchy are located in Eastern China, cities in the center and the west of the country are also gaining higher positions in the hierarchy over time. This paper is dedicated to improving the traditional GM in the applications of urban studies, while the system of Chinese cities is used to validate the SGM.

关键词： Chinese urban system Synthesized gravity model Principal component analysis Network analysis

Hybrid MARTE statecharts

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Frontiers of Computer Science 2013年第1期7卷 95-108页

作者： Jing liu ziwei liu Jifeng HE Freederic MALLET Zuohua DING Shanghai Key Laboratory of Trustworthy Computing East China Normal University Shanghai 200062 China INRIA Sophia Antipolis M6diterran6e Sophia Antipolis 06902 France Center of Math Computing and Software Engineering Zhejiang Sci-Tech University Hangzhou 310018 China

The specification of modeling and analysis of real-time and embedded systems （MARTE） is an extension of the unified modeling language （UML） in the domain of real-time and embedded systems. Even though MARTE time model offers a support to describe both discrete and dense clocks, the biggest effort has been put so far on the specifi- cation and analysis of discrete MARTE models. To address hybrid real-time and embedded systems, we propose to ex- tend statecharts using both MARTE and the theory of hybrid automata. We call this extension hybrid MARTE statecharts. It provides an improvement over the hybrid automata in that： the logical time variables and the chronometric time vari- ables are unified. The formal syntax and semantics of hybrid MARTE statecharts are given based on labeled transition sys- tems and live transition systems. As a case study, we model the behavior of a train control system with hybrid MARTE statecharts to demonstrate the benefit.

关键词： UML MARTE hybrid automata hybrid MARTE statechart train control system

Power-Domain Collision-Tolerant Random Access Method with Auxiliary Beam for Satellite Internet of Things:A New Solution

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China Communications 2024年第8期21卷 236-248页

作者： Xu Yuanyuan liu ziwei Bian Dongming Zhang Gengxin School of Communications and Information Engineering Nanjing University of Posts and TelecommunicationsNanjing 210003China Institute of Communications Engineering PLA University of Science and TechnologyNanjing 210007China

There are numerous terminals in the satellite Internet of Things(IoT).To save cost and reduce power consumption,the system needs terminals to catch the characteristics of low power consumption and light *** regular random access(RA)protocols may generate large amounts of collisions,which degrade the system throughout *** near-far effect and power control technologies are not applicable in capture effect to obtain power difference,resulting in the collisions that cannot be *** fact,the optimal design at the receiving end can also realize the condition of packet power domain separation,but there are few relevant *** this paper,an auxiliary beamforming scheme is proposed for power domain signal *** adds an auxiliary reception beam based on the conventional beam,utilizing the correlation of packets in time-frequency domain between the main and auxiliary beam to complete signal *** roll-off belt of auxiliary beam is used to create the carrier-to-noise ratio(CNR)*** paper uses the genetic algorithm to optimize the auxiliary beam *** results show that the proposed scheme outperforms slotted ALOHA(SA)in terms of system throughput per-formance and without bringing terminals additional control burden.

关键词： beamforming non-orthogonal multiple access random access satellite Internet of Things

Abnormal characteristics and effectiveness evaluation of the micro-seismic signal before the Debao MS4.8 earthquake

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Geodesy and Geodynamics 2023年第6期14卷 605-613页

作者： Jin Wei Huining Huang Ying Jiang ziwei liu Key Laboratory of Earthquake Geodesy China Earthquake AdministrationWuhan 430071China Earthquake Administration of Guangxi Zhuang Autonomous Region Nanning 530022China

The Debao MS4.8 earthquake occurred in western Guangxi on August 5,2021,near where the Jingxi MS5.2 earthquake occurred in *** study the increasing seismicity in western Guangxi,it is necessary to determine whether there was an anomaly related to the earthquake source near the Pingxiang gravity station,which is located approximately 100 km from the epicenter of the Debao MS4.8 *** this study,the R-value scoring method was used to analyze the anomaly and evaluate the prediction efficiency of the double frequency(DF)micro-seismic signal vertical displacement(referred to as vertical displacement,VD)and the absolute value of monthly extreme rate(referred to as the monthly rate).Results show that earthquakes larger than MS4.0 in the 350 km range from the Pingxiang station tend to coincide with yearly typhoons,and the VD of micro-seismic signals correspondingly changes from low to *** Debao MS4.8 earthquake occurred during a gradual VD increase from 0.05×10^(-6)to 0.10×10^(-6)*** discussing the relationships among R,the rate threshold,and the effective duration of prediction,the rate threshold of the micro-seismic signal converges from 0.00039×10^(-6)to 0.00031×10^(-6)m/month,the effective duration of prediction is approximately 6-10 months,and R also converges from 0.29 to *** comparing the results of three gPhone gravity stations in Guangxi,we found that the increase of short-term VD before the Debao earthquake was related to the enhancement of the DF micro-seismic signal excited by the *** the typhoon track was perpendicular to the coastline of China,the possibility of an earthquake occurring was *** study provides evidence and reference for the future occurrence period of earthquakes above MS4.0 in western Guangxi.

关键词： gPhone gravimeter ASSM Double frequency micro-seism R-value

Estimating the annual runoff frequency distribution based on climatic conditions and catchment characteristics:A case study across China

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International Soil and Water Conservation Research 2023年第3期11卷 470-481页

作者： ziwei liu Hanbo Yang Taihua Wang Dawen Yang State Key Laboratory of Hydro-Science and Engineering Department of Hydraulic EngineeringTsinghua UniversityBeijing100084China

Estimation of the annual runoff frequency distribution is an essential basis for water resource *** study proposes a framework for estimating the annual runoff frequency distribution across252 catchments in China based on climatic conditions and catchment characteristics from 1956 to *** Budyko land-specific parameter n,which intergrates influences other than the mean climate conditions,is firstly estimated based on easily ascertainable catchment characteristics without the requirementsof having long-term runoff ***,the annual runoff statistical parameters,namely,the mean value and standard deviation(STD),are derived based on the Budyko rainfall-runoffmodel with the central moment ***,the annual runoff on any recurrence interval is obtainedby the Pearson-Ill frequency *** show that the parameter n can be estimated fromthe catchment average slope,longitude,and climatic seasonality *** estimated statistical parametersof annual runoff have acceptable agreement with observed values(mean value:R^(2)-0.94,STD:R^(2)-0.91,and both relative errors<10%).In addition,estimated annual runoff at each catchment fortypical wet and dry years(25%and 75%ranked percentiles)coincides well with observed values,with R^(2) of 0.92-0.93 and relative erors less than 10%.This result indicates the robustness of this framework forestimating the annual runoff frequency distribution,which provides a simple and effective tool forungauged orpoorlygauged catchments.

关键词： runoff distribution climatic

Investigation of microstructure changes in Al_(2)O_(3)-YSZ coatings and YSZ coatings and their effect on thermal cycle life

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Journal of Advanced Ceramics 2022年第2期11卷 345-353页

作者： Meiqi DAI Xuemei SONG Chucheng LIN ziwei liu Wei ZHENG Yi ZENG The State Key Lab of High Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of SciencesShanghai 200050China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

Yttria-stabilized zirconia(YSZ)coatings and Al_(2)O_(3)–YSZ coatings were prepared by atmospheric plasma spraying(APS).Their microstructural changes during thermal cycling were investigated via scanning electron microscopy(SEM)equipped with electron backscatter diffraction(EBSD)and X-ray diffraction(XRD).It was found that the microstructure and microstructure changes of the two coatings were different,including crystallinity,grain orientation,phase,and phase *** differences are closely related to the thermal cycle life of the *** is a relationship between crystallinity and crack *** in grain orientation are related to microscopic strain and *** transition is the direct cause of coating *** this study,the relationship between the changes in the coating microstructure and the thermal cycle life is discussed in *** failure mechanism of the coating was comprehensively analyzed from a microscopic perspective.

关键词： thermal barrier coatings(TBCs) thermal cycling microstructure change microscopic strain failure mechanism