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Angelica sinensis polysaccharides ameliorate 5-flourouracil-induced bone marrow stromal cell proliferation inhibition via regulating Wnt/β-catenin signaling

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BIOCELL 2021年第4期45卷 1045-1058页

作者： HANXIANZHI XIAO RONGJIA QI ziling wang MINGHE XIAO YUE XIANG YAPING wang LU wang Laboratory of Stem Cell and Tissue Engineering Department of Histology and EmbryologyChongqing Medical UniversityChongqing40012China

Chemotherapy may cause cellular oxidative stress to bone *** damage of bone marrow hematopoietic microenvironment is closely related to chronic myelosuppression after chemotherapeutic *** sinensis polysaccharides(ASP)are major effective ingredients of traditional Chinese medicine Angelica with multi-target anti-oxidative stress *** the current study,we investigated the protective roles and mechanisms of ASP on chemotherapy-induced bone marrow stromal cell(BMSC)*** human bone marrow stromal cell line HS-5 cells were divided into control group,5-FU group,5-FU+ASP group,and 5-FU+LiCl group to investigate the mechanism of ASP to alleviate 5-FU-induced BMSC proliferation *** results showed that 5-FU inhibits the growth of HS-5 cells in a time and dose-dependent manner;however,ASP partially counteracted the 5-FU-induced decrease in cell viability,whereas Wnt signaling inhibitor Dkk1 antagonized the effect of ASP on HS-5 *** reversed the decrease in total cytoplasmicβ-catenin,p-GSK-3β,and CyclinD1 following 5-FU treatment and modulated nuclear expression ofβ-catenin,Lef-1,and C-myc ***,ASP also enhanced the antioxidant capacity of cells and reduced 5-FU-induced oxidative stress,attenuated FoxO1 expression,thus weakened its downstream apoptosis-related proteins and G0/G1 checkpoint-associated p27^(Kip1) expression to alleviate 5-FU-induced apoptosis and to promote cell cycle *** the results above suggest that the protective role of ASP in 5-FU-treated BMSCs proliferation for the chemotherapy may be related to its activating Wnt/β-catenin signaling and keeping homeostasis betweenβ-catenin and FoxO1 under oxidative *** study provides a potential therapeutic strategy for alleviating chemotherapeutic damage on BMSCs.

关键词： Angelica sinensis polysaccharides 5-Fluorouracil Wnt/β-catenin signaling pathway Oxidative stress Cell proliferation FoxO1

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Characterization of a Lacustrine Shale Reservoir and the Evolution of its Nanopores: A Case Study of the Upper Cretaceous Qingshankou Formation in the Songliao Basin, Northeastern China

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Acta Geologica Sinica(English Edition) 2020年第2期94卷 337-351页

作者： ZHANG Xu LIU Chenglin LI Bing WU Linqiang GUI Herong wang ziling ZHANG Zhihui LIANG Dexiu State Key Laboratory of Petroleum Resource and Prospecting China University of PetroleumBeijing 102249China College of Geosciences China University of Petroleum.Beijing 102249China National Engineering Research Center of Coal Mine Water Hazard Controlling(Suzhou University) SuzhouAnhui234000China Geological Exploration Technology Institute of Anhui Province Hefei 230031China Development and Research Center China Geological SurveyBeijing 100037China

The Songliao Basin is one of the most important petroliferous basins in northern China. With a recent gradual decline in conventional oil production in the basin, the exploration and development of unconventional resources are becoming increasingly urgent. The Qingshankou Formation consists of typical Upper Cretaceous continental strata, and represents a promising and practical replacement resource for shale oil in the Songliao Basin. Previous studies have shown that low-mature to mature Qingshankou shale mainly preserves type Ⅰ and type Ⅱ1 organic matter, with relatively high total organic carbon(TOC) content. It is estimated that there is a great potential to explore for shale oil resources in the Qingshankou Formation in this basin. However, not enough systematic research has been conducted on pore characteristics and their main controlling factors in this lacustrine shale reservoir. In this study, 19 Qingshankou shales from two wells drilled in the study area were tested and analyzed for mineral composition, pore distribution and feature evolution using Xray diffraction(XRD), scanning electron microscopy(SEM), low-pressure nitrogen gas adsorption(N2-GA), and thermal simulation experiments. The XRD results show that clay, quartz, and feldspar are the dominant mineral constituents of Qingshankou shale. The clay minerals are mostly illite/smectite mixed layers with a mean content of 83.5%, followed by illite, chlorite, and kaolinite. There are abundant deposits of clay-rich shale in the Qingshankou Formation in the study area, within which many mineral and organic matter pores were observed using SEM. Mineral pores contribute the most to shale porosity;specifically, clay mineral pores and carbonate pores comprise most of the mineral pores in the shale. Among the three types of organic matter pores, type B is more dominant the other two. Pores with diameters greater than 10 nm supply the main pore volume;most are half-open slits and wedge-shaped pores. The total por

关键词： lacustrine shale pore structure pore evolution Upper Cretaceous Songliao Basin

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Study on the thermal decomposition characteristics of C_(4)F_(7)N-CO_(2)mixture as ecofriendly gas-insulating medium

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High Voltage 2020年第1期5卷 46-52页

作者： Yi Li Xiaoxing Zhang Ji Zhang Cheng Xie Xianjun Shao ziling wang Dachang Chen Song Xiao School of Electrical Engineering and Automation Wuhan UniversityWuhan 430072People's Republic of China School of Electrical and Electronic Engineering Hubei University of TechnologyWuhan430068People's Republic of China State Grid Zhejiang Electric Power Research Institute Hangzhou310007People's Republic of China

The authors explored the thermal decomposition characteristics of perfluoroisobutyronitrile–carbon dioxide(C_(4)F_(7)N–CO_(2))gas mixture as eco-friendly dielectric *** main by-products and decomposition mechanism of C_(4)F_(7)N–CO_(2)gas mixture under different temperature and gas pressure conditions were revealed and *** was found that the thermal decomposition of 6%C_(4)F_(7)N–94%CO_(2)gas mixture starts at about 350°C(0.15 MPa),producing C_(3)F_(6) and CO *** other characteristic by-products such as CF_(4),C_(2)F_(6),CF_(3)CN,COF_(2) and(CN)_(2) could also be detected at higher *** yield of C_(3)F_(6),(CN)_(2) increased with the temperature(lower than 450℃)first and then decreased when it reached to 500℃.While the yield of CO,C3F8,COF_(2) and CF_(3)CN increased with temperature(350–550℃).The generation of CF_(4) and C_(2)F_(6) begins at temperatures higher than 500℃,which can be used as the feature component of severe overheating *** thermal decomposition amount and by-products yield of C_(4)F_(7)N–CO_(2)gas mixture slowed down with the increase of gas pressure,indicating that C_(4)F_(7)N–CO_(2)gas mixture is quite suitable used at high-pressure equipment,especially high-voltage devices such as gas insulated switchgear.

关键词： mixture thermal characteristics

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Career Planning Education Paths for Students of Aquatic Animal Medicine Discipline in the Context of the Belt and Road Initiative: A Case Study of Construction Achievement of Guangdong Ocean University

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Asian Agricultural Research 2019年第5期11卷 97-106页

作者： ziling wang Huanying PANG Shuanghu CAI Jichang JIAN Guangdong Ocean University

In order to improve the professional competitiveness of students in the Aquatic Animal Medicine Discipline,combined with the case tracking and theoretical practice for aquatic animal medicine students for three years,with the aid of SPSS software,this paper elaborated the actual level and existing problems in career planning of aquatic animal medicine students in Guangdong Ocean University from five dimensions:career planning awareness,self-awareness,environmental awareness,goal planning,and implementation correction. Then,it discussed the opportunities and challenges of the Aquatic Animal Medicine Discipline in the context of the Belt and Road Initiative. Finally,it came up with solutions and reform recommendations in education,teaching,and counseling paths from the students,university,families,government,and society.

关键词： Aquatic animal medicine Career planning The Belt and Road Initiative

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SEREX方法鉴定CTCL细胞肿瘤抗原

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解放军医学杂志 2007年第10期32卷 1057-1060页

作者：李方王字玲解放军总医院肿瘤科北京100853 军事医学科学院野战输血研究所

目的分离纯化皮肤T细胞淋巴瘤(CTCL)细胞系SeAx细胞膜蛋白并制备多克隆抗体。利用SEREX法从SeAx cDNA噬菌体表达文库中筛选肿瘤抗原。方法培养SeAx细胞至2×1010个,用组织匀浆器匀浆后差速离心法分离细胞各组分,富集细胞膜抗原。用细胞... 详细信息

目的分离纯化皮肤T细胞淋巴瘤(CTCL)细胞系SeAx细胞膜蛋白并制备多克隆抗体。利用SEREX法从SeAx cDNA噬菌体表达文库中筛选肿瘤抗原。方法培养SeAx细胞至2×1010个,用组织匀浆器匀浆后差速离心法分离细胞各组分,富集细胞膜抗原。用细胞器特异性抗体对纯化步骤中的各组分进行检测。用膜蛋白组分免疫家兔4次,每次免疫后用ELISA法测定血清抗体滴度。4次免疫后用Western blot方法测定多抗与SeAx细胞各组分蛋白反应的特异性。重组噬菌体感染大肠杆菌,将表达的重组蛋白转印到硝酸纤维素膜上,与SeAx细胞膜蛋白免疫的兔血清进行反应,阳性的克隆测定核苷酸序列,通过数据库搜索比较确定基因。结果用差速离心法得到SeAx细胞膜蛋白,免疫家兔4次后获得了效价为1×10-5的多克隆抗体。共筛选了1×106个噬菌体克隆,得到25个阳性克隆,经核苷酸序列测定和数据库分析,其中4个cDNA片段为膜蛋白成分,分别为integrin alpha 4,ligatin,matrix metalloproteinase 24和MHC-I molecule HLA-A。结论分离并纯化了CTCL细胞系SeAx细胞膜蛋白,成功制备了多克隆抗体,用SEREX方法从SeAxmRNA建立的cDNA噬菌体表达文库中筛选到4个肿瘤细胞膜蛋白基因,它们可以引起机体产生体液免疫反应,并可能成为潜在的免疫治疗靶点。

关键词：淋巴瘤 SEREX 抗体多克隆抗原肿瘤

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The inhibition of melanoma metastasis in C57BL/6 mice mediated by allo-reactive NK cell transfusion

The inhibition of melanoma metastasis in C57BL/6 mice mediat...

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中国生物化学与分子生物学会第十届会员代表大会暨全国学术会议

作者： Yongyong Tang Wenyuan Duan Jingxiang Zhao ziling wang Beijing Institute of Tansfusion Medicine Academy of Military Medical Sciences

Objective To Study the anti-tumor activity of the alloreactive NK cells(H-2d) in C57BL/6 H-2b *** NK cells were isolated and purified by anti-DX5 microbeads. The melanoma pulmonary metastatic mouse model was established by intravenous injection of B16F10(H-2b) cells to C57BL/6 H-2b *** the mice were randomly divided into three groups and the same volume of Syn-NK cells from C57BL/6 H-2b mice,Allo-NK cells from BALB/C H-2d mice and PBS which were regarded as control were transfused *** number of the melanoma

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RNA m^(5)C修饰调控病毒复制的研究进展

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科学通报 2022年第31期67卷 3654-3666页

作者：张婷王紫凌郑永唐中国科学院昆明动物研究所中国科学院动物模型与人类疾病机理重点实验室昆明650223 中国科学技术大学生命科学学院合肥230026

5-甲基胞嘧啶(5-methylcytosine,m^(5)C)作为一类重要的转录后修饰形式,在真核生物信使RNA(messenger RNA,mRNA)、转运RNA(transfer RNA,tRNA)和核糖体RNA(ribosomal RNA,rRNA)上普遍存在,并且参与调控RNA出核、翻译和稳定性维持等代谢加工过程.该修饰是动态可逆的,由甲基转移酶(methyltransferases)催化生成,并由去甲基化酶(demethylases)移除,通过招募特定的识别蛋白(reader proteins,readers)参与调控胚胎发育、肿瘤发展和干细胞分化等生理及病理过程.近期研究表明,除真核生物RNA外,病毒RNA上同样富含m^(5)C修饰,并且在转录、剪切和翻译等复制阶段扮演重要角色.此外,病毒感染可诱发宿主RNA的m^(5)C修饰改变,从而影响宿主对病毒感染的应答反应.随着RNA m^(5)C修饰测序技术的快速发展,病毒m^(5)C修饰的相关报道大量涌现,然而m^(5)C修饰在病毒感染中的具体调控机制至今尚未完全阐明.本文综述了近年来RNA m^(5)C修饰在逆转录病毒(retrovirus)、DNA病毒(DNA virus)、黄病毒(flavivirus)和冠状病毒(coronavirus)复制,以及宿主抗病毒免疫应答中的调控作用和分子机制,旨在为病毒表观遗传学研究提供参考.

关键词： 5-甲基胞嘧啶甲基转移酶去甲基化酶识别蛋白病毒复制宿主抗病毒免疫反应

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The selective regulation of NKG2D ligands by 5-FU and MG132

The selective regulation of NKG2D ligands by 5-FU and MG132

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中国生物化学与分子生物学会第十届会员代表大会暨全国学术会议

作者： Dan Luo Jingxiang Zhao Yan Zhang Guangzhi Wei ziling wang Beijing Institute of Tansfusion Medicine Academy of Military Medical Sciences

Objective:The research is to study the regulation effect of 5-FU and MG132 on the expression of NKG2D ligands on A549 cell lines and the mechanisms ***:The mRNA expression of NKG2D ligands as well as the cellsurface expression levels of NKG2D ligands were measured by Dual-Luciferase Reporter Assay,fluorescence quantitative polymerase chain reaction(PCR) and Flow cytometry *** influssion of 5-FU and MG132 on the NK cells-mediated killing of A549 cells was determined by MTT *** order to investigate whether DNA damage was occurred after the treatment with 5-FU and MG132,We also performed comet assay to observe the DNA *** for

关键词： 5-FU MG132 NKG2D ligands expression regulation

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The regulatory mechanism of MICA expression on the surface of Hela cells

The regulatory mechanism of MICA expression on the surface o...

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中国生物化学与分子生物学会第十届会员代表大会暨全国学术会议

作者： Yan Zhang Guangzhi Wei Jingxiang Zhao Dan Luo ziling wang Beijing Institute of Tansfusion Medicine Academy of Military Medical Sciences

Objective To investigate the regulatory mechanism of surface expression of MICA in Hela cells,focusing on the role of the cytoplasmic *** HeLa cells were treated with different concentrations of 5-Fu and then the changes of RNA and protein of MICA were detected by fluorescence quantitative PCR or flow *** MICA-full-length and cytoplasmic tail truncated MICA were amplified and inserted into expression *** cells were transfected by the constructed vectors and treated with 5-Fu(40ug/ml) for 24 hours or/and MG132 for 8 hours.

关键词： 5-fluorouraci MICA HeLa cells cytoplasmic tail

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Reform and Exploration of Integrating"Service Learning"into the Teaching of Pathogenic Biology of Aquatic Animals to Help Rural Revitalization

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Asian Agricultural Research 2023年第1期15卷 58-61页

作者： Huanying PANG Miao XIE Shuanghu CAI ziling wang Shiping YANG Yucong HUANG Jichang JIAN College of Fisheries Guangdong Ocean UniversityZhanjiang 524088China National Experimental Teaching Demonstration Center of Fishery Science and Technology Zhanjiang 524088China

This paper intends to combine the development trend of the subject,restructure and optimize the course content,and construct the service learning model of Pathogenic Biology of Aquatic Animals for *** the same time,through service practice,it is needed to further consolidate curriculum knowledge and skills,stimulate the learning initiative and enthusiasm of postgraduates,expand professional knowledge,improve professional quality,and lay a solid foundation for serving the national rural revitalization strategy in the future.

关键词： Pathogenic Biology of Aquatic Animals Service learning Rural revitalization

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