检索结果-南通市图书馆

Genomics, Proteomics & Bioinformatics 2021年第6期19卷 873-881页

作者： Yangyang Li Zonggui Chen Hairong Liu Qiming Li Xing Lin Shuhui Ji Rui Li Shaopeng Li Weiliang Fan Haiping Zhao zuoyan zhu Wei Hu Yu Zhou Daji Luo State Key Laboratory of Virology College of Life SciencesWuhan UniversityWuhan 430072China State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyInnovation Academy for Seed DesignChinese Academy of SciencesWuhan 430072China School of Basic Medical Science Wuhan UniversityWuhan 430071China University of Chinese Academy of Sciences Beijing 100049China Frontier Science Center for Immunology and Metabolism Wuhan UniversityWuhan 430072China

Sex reversal,representing extraordinary sexual plasticity during the life cycle,not only triggers reproduction in animals but also affects reproductive and endocrine system-related diseases and cancers in *** reversal has been broadly reported in animals;however,an integrated resource hub of sex reversal information is still ***,we constructed a comprehensive database named ASER(Animal Sex Reversal)by integrating sex reversal-related data of 18 species from teleostei to *** systematically collected 40,018 published papers and mined the sex reversal-associated genes(SRGs),including their regulatory networks,from 1611 core *** annotated homologous genes and computed conservation scores for whole genomes across the 18 ***,we collected available RNA-seq datasets and investigated the expression dynamics of SRGs during sex reversal or sex determination *** addition,we manually annotated 550 in situ hybridization(ISH),fluorescence in situ hybridization(FISH),and im-munohistochemistry(IHC)images of SRGs from the literature and described their spatial expression in the ***,ASER provides a unique and integrated resource for researchers to query and reuse organized data to explore the mechanisms and applications of SRGs in animal breeding and human *** ASER database is publicly available at http://***/.

关键词： Sex reversal Sex reversal-associated gene Database Omics Conservation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Single-cell analysis reveals an Angpt4-initiated EPDC-EC-CM cellular coordination cascade during heart regeneration

引用

Protein & Cell 2023年第5期14卷 350-368页

作者： Zekai Wu Yuan Shi Yueli Cui Xin Xing Liya Zhang Da Liu Yutian Zhang Ji Dong Li Jin Meijun Pang Rui-Ping Xiao zuoyan zhu Jing-Wei Xiong Xiangjun Tong Yan Zhang Shiqiang Wang Fuchou Tang Bo Zhang Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education Peking University Genome Editing Research CenterCollege of Life SciencesPeking UniversityBeijing 100871China Beijing Advanced Innovation Center for Genomics(ICG) College of Life SciencesPeking UniversityBeijing 100871China State Key Laboratory of Membrane Biology Institute of Molecular MedicineCollege of Future TechnologyPeking UniversityBeijing 100871China State Key Laboratory of Membrane Biology College of Life SciencesPeking UniversityBeijing 100871China Institute of Cardiovascular Sciences and Key Laboratory of Molecular Cardiovascular Sciences School of Basic Medical SciencesMinistry of EducationPeking University Health Science CenterBeijing 100191China Institute of Molecular Medicine College of Future TechnologyPeking UniversityBeijing 100871China

Mammals exhibit limited heart regeneration ability,which can lead to heart failure after myocardial *** contrast,zebrafish exhibit remarkable cardiac regeneration *** cell types and signaling pathways have been reported to participate in this ***,a comprehensive analysis of how different cells and signals interact and coordinate to regulate cardiac regeneration is *** collected major cardiac cell types from zebrafish and performed high-precision single-cell transcriptome analyses during both development and post-injury *** revealed the cellular heterogeneity as well as the molecular progress of cardiomyocytes during these processes,and identified a subtype of atrial cardiomyocyte exhibiting a stem-like state which may transdifferentiate into ventricular cardiomyocytes during ***,we identified a regeneration-induced cell(RIC)population in the epicardium-derived cells(EPDC),and demonstrated Angiopoietin 4(Angpt4)as a specific regulator of heart ***4 expression is specifically and transiently activated in RIC,which initiates a signaling cascade from EPDC to endocardium through the Tie2-MAPK pathway,and further induces activation of cathepsin K in cardiomyocytes through RA *** of angpt4 leads to defects in scar tissue resolution and cardiomyocyte proliferation,while overexpression of angpt4 accelerates ***,we found that ANGPT4 could enhance proliferation of neonatal rat cardiomyocytes,and promote cardiac repair in mice after myocardial infarction,indicating that the function of Angpt4 is conserved in *** study provides a mechanistic understanding of heart regeneration at single-cell precision,identifies Angpt4 as a key regulator of cardiomyocyte proliferation and regeneration,and offers a novel therapeutic target for improved recovery after human heart injuries.

关键词： scRNA-seq zebrafish heart regeneration Angpt4 EPDC

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

美国转基因大西洋鲑产业化对我国的启示

引用

中国工程科学 2016年第3期18卷 105-109页

作者：胡炜朱作言中国科学院水生生物研究所淡水生态与生物技术国家重点实验室武汉430072

2015年11月19日,美国食品药品监督管理局(FDA)批准了转全鱼生长激素基因(gh)大西洋鲑为第一种可供食用的转基因动物产品,从而取得世界转基因动物育种研究与产业化的历史性突破。经过了长达20年的严格审核,转gh大西洋鲑才获准产业化,不... 详细信息

2015年11月19日,美国食品药品监督管理局(FDA)批准了转全鱼生长激素基因(gh)大西洋鲑为第一种可供食用的转基因动物产品,从而取得世界转基因动物育种研究与产业化的历史性突破。经过了长达20年的严格审核,转gh大西洋鲑才获准产业化,不仅充分说明了对包括转基因鱼在内的创新产业的管理决策尤其需要尊重科学规律和客观事实;而且表明基础研究只是产业创新的因素之一,转基因知识的科学普及与企业的积极参与是推动高新技术发展和创新产业形成不可或缺的重要条件;高屋建瓴地制定我国基因改良农业品种市场准入的条例和操作细则已经刻不容缓。

关键词：转基因大西洋鲑产业化启示

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

斑马鱼胚胎中Cre/lox介导的定点整合

引用

高技术通讯 2007年第10期17卷 1072-1076页

作者：秦瑶汪亚平胡炜朱作言中国科学院水生生物研究所淡水生态与生物技术国家重点实验室武汉430072 中国科学院研究生院北京100039

采用两种类型的lox突变体和双色荧光报告基因,构建了双交换载体pCMVlox66-CreintEGFPlox2272和plox71RFPlox2272。将Cre酶基因插入载体pCS2＋,获得了体外转录模板质粒pCSCre。为检测Cre重组酶对斑马鱼胚胎发育的影响,将体外转录的Cre RN... 详细信息

采用两种类型的lox突变体和双色荧光报告基因,构建了双交换载体pCMVlox66-CreintEGFPlox2272和plox71RFPlox2272。将Cre酶基因插入载体pCS2＋,获得了体外转录模板质粒pCSCre。为检测Cre重组酶对斑马鱼胚胎发育的影响,将体外转录的Cre RNA稀释至不同浓度并分别注射入斑马鱼受精卵。结果显示,当Cre RNA浓度高于200ng/μL时会导致高死亡率和高畸形率;而浓度低于100ng/μL的Cre RNA对胚胎发育不造成影响。进一步将50ng/μL的Cre RNA同双交换载体共注射到斑马鱼受精卵中,筛选到发红色荧光的胚胎。PCR检测和测序鉴定证实,在Cre重组酶作用下,两个载体中lox位点间的荧光基因发生了置换反应。实验结果表明,Cre/lox系统介导的盒式交换反应是实现转基因鱼外源基因定点整合的有效策略。

关键词： Cre重组酶斑马鱼定点整合盒式交换

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Transgenes in F4 pMThGH-transgenic common carp (Cy-prinus carpio L.) are highly polymorphic

Transgenes in F4 pMThGH-transgenic common carp (Cy-prinus ca...

引用

第六届动物遗传学讨论会

作者： ZENG Zhiqiang & zhu zuoyan State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China Correspondence should be addressed to zhu zuoyan (e-mail: zyzhu@***)

To gain information on the integration pattern of pMThGH-transgene, 50 transgenes were recovered from F4 generation of pMThGH transgenic common carp (Cyprinus carpio L.) and 33 recovered genes were analyzed. The restriction maps of these recovered genes were constructed by digestion with five kinds of enzymes. These transgenes can be classified into 4 types according to their restriction maps. Only one type of transgenes maintains its original molecular form, whereas the other three types are very different from me original one and vary each other on both molecular weight and restriction maps. This implies that the sequences of most transgenes have been deleted and/or rearranged during integration and inheritance. The results of PCR amplification and Southern blot hybridization indicate mat MThGH in Type I transgene keeps intact but most of its sequence has been deleted in other three types. All these results suggest that transgenes in F4 generation of transgenic carp are highly polymorphic. Two DNA fragments concerning integration site of transgenes were cloned from recovered transgenes, and found to be homologous to the 5’UTR of B-actin gene of common carp and mouse mRNA for receptor tyrosine kinase (RTK), respectively.

关键词： transgenic common carp integration pattern transgene recovery.

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Molecular cloning of growth hormone receptor (GHR) from common carp(Cyprinus carpio L.)and identification of its two forms of mRNA transcripts

引用

Progress in Natural Science:Materials International 2006年第11期16卷 1156-1163页

作者： SUN Xiaofeng~ 1,2 , GUO Qionglin~1**, HU Wei~1, WANG Yaping~1 and zhu zuoyan~1(1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China 2. Graduate School of Chinese Academy of Sciences, Beijing 100039, China) State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Graduate School of Chinese Academy of Sciences Beijing 100039 China

The cDNA of growth hormone receptor (GHR) was cloned from the liver of 2-year common carp (Cyprinus carpio L.) by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE). Its open reading frame (ORF) of 1806 nucleotides is translated into a putative peptide of 602 amino acids, including an extracellular ligand-binding domain of 244 amino acids (aa), a single transmembrane domain of 24 aa and an intracellular signal-transduction domain of 334 aa. Sequence analysis indicated that common carp GHR is highly homologous to goldfish (Carassius auratus) GHR at both gene and protein levels. Using a pair of gene-specific primers, a GHR fragment was amplified from the cDNA of 2-year common carp, a 224 bp product was identified in liver and a 321 bp product in other tissues. The sequencing of the products and the partial genomic DNA indicated that the difference in product size was the result of a 97 bp intron that alternatively spliced. In addition, the 321 bp fragment could be amplified from all the tissues of 4-month common carp including liver, demonstrating the occurrence of the alternative splicing of this intron during the development of common carp. Moreover, a semi-quantitative RT-PCR was performed to analyze the expression level of GHR in tissues of 2-year common carp and 4-month common carp. The result revealed that in the tissues of gill, thymus and brain, the expression level of GHR in 2-year common carp was significantly lower than that of 4-month common carp.

关键词： growth hormone receptor (GHR) molecular cloning transcript expression analysis common carp.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

转基因鱼的研究进展

引用

农业生物技术学报 1999年第3期7卷 298-303页

作者：赵浩斌陈尚萍朱作言武汉430072 中国科学院水生生物研究所!淡水生态与生物技术国家重点实验室

自１９８４年第一例转基因鱼在我国诞生以来，转基因鱼研究取得长足进步。研究表明外源基因可以整合、表达，并能遗传给后代，然而外源基因整合位点难以控制，遗传不稳定等问题仍未解决。所幸我国鱼类核移植技术较为成熟，结合细胞基... 详细信息

自１９８４年第一例转基因鱼在我国诞生以来，转基因鱼研究取得长足进步。研究表明外源基因可以整合、表达，并能遗传给后代，然而外源基因整合位点难以控制，遗传不稳定等问题仍未解决。所幸我国鱼类核移植技术较为成熟，结合细胞基因转移、基因打靶和核移植技术，有望在不久将来获得稳定整合的纯化转基因鱼。

关键词：鱼细胞基因转移胚胎干细胞核移植学科分类号

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Genetic analysis of "all-fish" growth hormone gene transferred carp (Cyprinus carpio L.) and its F_1 generation

引用

Chinese Science Bulletin 2001年第14期46卷 1174-1178页

作者： WANG Yaping, HU Wei, WU Gang,SUN Yonghua, CHEN Shangping, ZHANG Fuying,zhu zuoyan, FENG Jianxin & ZHANG Xirui1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China 2. Henan Institute of Aquaculture, Zhengzhou 450044, China State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan China Henan Institute of Aquaculture Zhengzhou China

Recornbinant "all-fish" growth hormone gene (GH) was microinjected into the fertilized eggs of carp. A comparison between the growth traits of transgenics and non-transgenics was carried out, and the transgenic individuals with significant "fast-growing" effect were successfully gained. A comparison on the reproductivities was also given out between the transgenics and their non-transgenic siblings, and showed that the reproductive capacity of transgenics was substantially equivalent to those of the non-transgenics. On the other hand, the genetic separation and the characteristic distribution of the FI generation were genetically analyzed, which gave solid evidence for the hypothesis that 2-3 chromosomes are integrated with trans-gene. In addition, the distinct biological effects for multi-site-integrated transgenes were further discussed. The present study opens a door for the breeding of "fast-growing" transgenic fish.

关键词： all-fish" growth hormone gene carp genetic analysis breeding.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

我国科技期刊改革实践与思考——以《中国科学》系列和《科学通报》期刊为例

引用

编辑学报 2019年第6期31卷 638-641页

作者：黄延红严谨彭斌朱作言《中国科学》杂志社北京100717 科学出版社北京100717 中国科学院水生生物研究所武汉430072

如何加快中国品牌的世界一流科技期刊建设,已经成为新时代科技期刊建设的重要研究课题。本文以《中国科学》系列和《科学通报》(以下简称为"两刊")为例,从优化期刊布局、推动学部平台办刊、发挥专家办刊作用、提升学术质量和核心竞争能... 详细信息

如何加快中国品牌的世界一流科技期刊建设,已经成为新时代科技期刊建设的重要研究课题。本文以《中国科学》系列和《科学通报》(以下简称为"两刊")为例,从优化期刊布局、推动学部平台办刊、发挥专家办刊作用、提升学术质量和核心竞争能力等方面介绍"两刊"的改革举措与发展实践,并从集群化、国际化、专业化和数字化4个方面对世界一流科技期刊建设提出思考与建议。

关键词：科技期刊集群化建设国际影响力数字出版

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cloning of rainbow trout (Oncorhynchus mykiss) histone H3 promoter and the activity analysis in rare minnow (Gobiocypris rarus )

引用

Progress in Natural Science:Materials International 2004年第4期 36-40+91页

作者： MAO Weifeng, SUN Yonghua, WANG Yaping, WU Gang, CHEN Shangping and zhu zuoyan(State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China)

Rainbow trout histone H3 (RH3) promoter was cloned via high fidelity PCR. The cloned RH3 promoter was inserted into a promoter-lacked vector pEGFP-1, resulting in an expression vector pRH3EGFP-1. The linearized pRH3EGFP-1 was microinjected into fertilized eggs of rare minnows and the sequential embryogenetic processes were monitored under a fluorescent microscope. Strong green fluorescence was ubiquitously observed at as early as the gastrula stage and then in various tissues at the fry stage. The results indicate that RH3 promoter, as a piscine promoter, could serve in producing transgenic Cyprinoid such as rare minnow. Promoter activity of RH3, CMV and common carp β-actin (CA) were compared in rare minnow by the expression of respective recombinant EGFP vectors. The expression of pCMVEGFP occurred earlier than the following one, pRH3EGFP-1, and then pCAEGFP during the embryogenesis of the transgenics. Their expression activities demonstrated that the CMV promoter is the strongest one, followed by the

关键词： enhanced green fluorescent protein (EGFP) histone H3 promoter transgenic fish rare minnow.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：