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Long-term in vivo chimeric cells tracking in non-human primate

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Protein & Cell 2024年第3期15卷 207-222页

作者： Junmo Wu Yu Kang Xiang Luo Shaoxing Dai Yuxi Shi zhuoyao Li Zengli Tang Zhenzhen Chen Ran zhu Pengpeng Yang zifan Li Hong Wang Xinglong Chen Ziyi Zhao Weizhi ji Yuyu Niu State Key Laboratory of Primate Biomedical Research Institute of Primate Translational MedicineKunming University of Science and TechnologyKunming 650500China Yunnan Key Laboratory of Primate Biomedical Research Kunming 650500China Faculty of Life Science and Technology Kunming University of Science and TechnologyKunming 650500China

Non-human primates(NHPs)are increasingly used in preclinical trials to test the safety and efficacy of biotech-nology therapies.Nonetheless,given the ethical issues and costs associated with this model,it would be highly advantageous to use NHP cellular models in clinical studies.However,developing and maintaining the naive state of primate pluripotent stem cells(PSCs)remains difficult as does in vivo detection of PSCs,thus limiting biotech-nology application in the cynomolgus monkey.Here,we report a chemically defined,xeno-free culture system for culturing and deriving monkey PSCs in vitro.The cells display global gene expression and genome-wide hypometh-ylation patterns distinct from monkey-primed cells.We also found expression of signaling pathways components that may increase the potential for chimera formation.Crucially for biomedical applications,we were also able to integrate bioluminescent reporter genes into monkey PsCs and track them in chimeric embryos in vivo and in vitro.The engineered cells retained embryonic and extra-embryonic developmental potential.Meanwhile,we generated a chimeric monkey carrying bioluminescent cells,which were able to track chimeric cells for more than 2 years in living animals.Our study could have broad utility in primate stem cell engineering and in utilizing chimeric monkey models forclinical studies.

关键词： pluripotent stem cells bioluminescence imaging non-human primates

Increasing prediction performance of colorectal cancer disease status using random forests classification based on metagenomic shotgun sequencing data

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Synthetic and Systems Biotechnology 2022年第1期7卷 574-585页

作者： Yilin Gao zifan zhu Fengzhu Sun Department of Quantitative and Computational Biology University of Southern California1050 Childs WayRRI 201Los AngelesCAUnited States

Dysfunction of microbial communities in various human body sites has been shown to be associated with a variety of diseases raising the possibility of predicting diseases based on metagenomic samples.Although many studies have investigated this problem,there are no consensus on the optimal approaches for predicting disease status based on metagenomic samples.Using six human gut metagenomic datasets consisting of large numbers of colorectal cancer patients and healthy controls from different countries,we investigated different software packages for extracting relative abundances of known microbial genomes and for integrating mapping and as-sembly approaches to obtain the relative abundance profiles of both known and novel genomes.The random forests(RF)classification algorithm was then used to predict colorectal cancer status based on the microbial relative abundance profiles.Based on within data cross-validation and cross-dataset prediction,we show that the RF prediction performance using the microbial relative abundance profiles estimated by Centrifuge is generally higher than that using the microbial relative abundance profiles estimated by MetaPhlAn2 and Bracken.We also develop a novel method to integrate the relative abundance profiles of both known and novel microbial or-ganisms to further increase the prediction performance for colorectal cancer from metagenomes.

关键词： Microbiome Colorectal cancer Metagenomic shotgun sequencing Random forests

Unhealthy Eating Behaviors During Pregnancy and Gestational Weight Gain—Huai’an City,Jiangsu Province,China,2020–2021

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China CDC weekly 2023年第35期5卷 773-778,I0002-I0004页

作者： zifan Zhang Weijie Ding Hui Wang Yangyang Cheng Yue Zhang Xiaoqin zhu Xiaolin Xu School of Public Health The Second Affiliated HospitalZhejiang University School of MedicineHangzhou CityZhejiang ProvinceChina Health Care Department Affiliated Hospital of Yangzhou University Huai’an Maternal and Child Health Care CenterHuai’an CityJiangsu ProvinceChina School of Public Health Faculty of MedicineThe University of QueenslandBrisbaneAustralia.

Summary What is already known about this topic?Maintaining a healthy diet and appropriate weight during pregnancy is crucial for both the expectant mother and the fetus.Unhealthy eating behaviors(UEBs)such as eating out frequently are becoming increasingly prevalent across the globe.However,there is a dearth of research investigating the relationship between UEBs and gestational weight gain(GWG)specifically in the context of Chinese women.

关键词： becoming mother Jiangsu

麝香保心丸“异病同治”2型糖尿病和冠心病的网络药理学作用机制研究

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药学实践与服务 2024年第4期42卷 173-180页

作者：朱子凡王智聪谢彬柳润辉福建中医药大学福建福州350122 海军军医大学药学系上海200433

目的基于网络药理学方法研究麝香保心丸“异病同治”2型糖尿病(T2DM)和冠心病(CHD)的作用机制。方法运用中药系统药理学数据库与分析平台(TCMSP)、中药成分数据库(TCMID)、中医药百科全书数据库(ETCM)和BATMAN-TCM数据库筛选麝香保心... 详细信息

目的基于网络药理学方法研究麝香保心丸“异病同治”2型糖尿病(T2DM)和冠心病(CHD)的作用机制。方法运用中药系统药理学数据库与分析平台(TCMSP)、中药成分数据库(TCMID)、中医药百科全书数据库(ETCM)和BATMAN-TCM数据库筛选麝香保心丸中七味中药的化学成分和作用靶点,利用DisGeNET和GeneCards数据库获取与CHD和T2DM相关的疾病靶点。运用Cytoscape3.8.2软件构建“药物-成分-靶点”网络图,利用STRING数据库构建蛋白互作(PPI)网络图,利用DAVID在线数据库对麝香保心丸治疗T2DM和CHD的共同作用靶点进行GO生物过程分析和KEGG通路富集分析。结果共筛选出麝香保心丸治疗T2DM和CHD的潜在活性成分101个,对应229个靶点。网络分析结果表明,麝香保心丸治疗T2DM和CHD的共同主要成分可能是鹅去氧胆酸、熊去氧胆酸、肉桂醛、胆汁酸、肉桂酸及人参皂苷类成分。通路富集分析结果显示,麝香保心丸“异病同治”T2DM和CHD的作用机制可能与抑制炎症反应和氧化应激有关。结论麝香保心丸可通过多成分、多靶点、多途径发挥“异病同治”T2DM和CHD的作用,为麝香保心丸的临床应用及进一步研究提供理论基础。

关键词：麝香保心丸 2型糖尿病冠心病异病同治网络药理学

高海拔地区建筑施工项目应急能力建设评价方法研究

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技术与创新管理 2024年第3期45卷 305-312页

作者：张佳朱武全成连华李梓凡郭慧敏中国建筑土木建设有限公司北京100070 西安科技大学安全科学与工程学院陕西西安710054

为科学评价高海拔地区建筑施工项目应急能力建设水平,将应急能力建设过程划分为三个阶段、7个重点建设任务,构建了高海拔地区建筑施工项目应急能力建设评价指标体系;充分考虑高海拔地区建筑施工特点,通过使用AHP与熵权法的组合赋权法确... 详细信息

为科学评价高海拔地区建筑施工项目应急能力建设水平,将应急能力建设过程划分为三个阶段、7个重点建设任务,构建了高海拔地区建筑施工项目应急能力建设评价指标体系;充分考虑高海拔地区建筑施工特点,通过使用AHP与熵权法的组合赋权法确定各指标权重分布;在此基础上,采用模糊综合评价理论,构建了高海拔地区建筑施工项目应急能力评价模型,实现了多因素评估问题从定性到定量分析的转变。以高海拔地区某建筑施工项目为研究对象,进行实例验证。结果表明:该项目应急能力建设中仍存在待提升部分,该评价指标体系可以胜任高海拔地区建筑施工项目应急能力建设水平评估问题,为高海拔地区应急决策提供一定的理论依据。

关键词：高海拔地区建筑施工应急能力建设组合权重模糊综合评价

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Energy band alignment of 2D/3D MoS_(2)/4H-SiC heterostructure modulated by multiple interfacial interactions

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Frontiers of physics 2023年第1期18卷 153-164页

作者： Huili zhu zifan Hong Changjie Zhou Qihui Wu Tongchang Zheng Lan Yang Shuqiong Lan Weifeng Yang Xiamen Key Laboratory of Ultra-Wide Bandgap Semiconductor Materials and Devices Department of PhysicsSchool of ScienceJimei UniversityXiamen 361021China Department of Microelectronics and Integrated Circuit School of Electronic Science and Engineering(National Model Microelectronics College)Xiamen UniversityXiamen 361005China School of Marine Equipment and Mechanical Engineering Jimei UniversityXiamen 361021China

The interfacial properties of MoS_(2)/4H-SiC heterostructures were studied by combining first-principles calculations and X-ray photoelectron spectroscopy.Experimental(theoretical)valence band offsets(VBOs)increase from 1.49(1.46)to 2.19(2.36)eV with increasing MoS_(2) monolayer(1L)up to 4 layers(4L).A strong interlayer interaction was revealed at 1L MoS_(2)/SiC interface.Fermi level pinning and totally surface passivation were realized for 4H-SiC(0001)surface.About 0.96e per unit cell transferring forms an electric field from SiC to MoS_(2).Then,1L MoS_(2)/SiC interface exhibits type I band alignment with the asymmetric conduction band offset(CBO)and VBO.For 2L and 4L MoS_(2)/SiC,Fermi level was just pinning at the lower MoS_(2)1L.The interaction keeps weak vdW interaction between upper and lower MoS_(2) layers.They exhibit the type II band alignments and the enlarged CBOs and VBOs,which is attributed to weak vdW interaction and strong interlayer orbital coupling in the multilayer MoS_(2).High efficiency of charge separation will emerge due to the asymmetric band alignment and built-in electric field for all the MoS_(2)/SiC interfaces.The multiple interfacial interactions provide a new modulated perspective for the next-generation electronics and optoelectronics based on the 2D/3D semiconductors heterojunctions.

关键词： MoS_(2) SiC X-ray photoelectron spectroscopy band alignment first-principles calculations

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环境审计与绿色发展关系研究:一个文献综述

财务管理研究 2024年第4期 5-9页

作者：周健强朱妍宇夏思源牟子凡长江大学经济与管理学院荆州434000

随着绿色发展地位的逐渐提高,与之相对应的评估检验方法显得越来越重要。作为审计工作的一个分支,环境审计为绿色发展创造了丰厚价值,其内在价值备受关注。但由于环境审计尚处于发展初期,在实际应用上仍存在诸多空白。通过文献综述,首... 详细信息

随着绿色发展地位的逐渐提高,与之相对应的评估检验方法显得越来越重要。作为审计工作的一个分支,环境审计为绿色发展创造了丰厚价值,其内在价值备受关注。但由于环境审计尚处于发展初期,在实际应用上仍存在诸多空白。通过文献综述,首先梳理国内外学者对环境审计和绿色发展内涵的研究成果;其次对二者的分析评价方法进行综述;最后基于现有研究提出对环境审计和绿色发展研究的展望。

关键词：环境审计绿色发展相关性

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基于自抗扰采样控制的车辆转向跟踪

聊城大学学报（自然科学版） 2024年第4期37卷 14-22页

作者：刘亚辉高子凡朱淑倩王庭瑞山东大学数学学院山东济南250100

运用自抗扰采样控制策略研究四轮转向车辆的模型参考跟踪控制问题。设计一个采样数据驱动的扩展状态观测器来同时估计跟踪误差和侧风扰动,并基于估计跟踪误差和估计干扰构建自抗扰采样控制器。利用增广系统和输入延迟系统建模方法,并结... 详细信息

运用自抗扰采样控制策略研究四轮转向车辆的模型参考跟踪控制问题。设计一个采样数据驱动的扩展状态观测器来同时估计跟踪误差和侧风扰动,并基于估计跟踪误差和估计干扰构建自抗扰采样控制器。利用增广系统和输入延迟系统建模方法,并结合Lyapunov-Krasovskii泛函方法,得到采样周期依赖的H∞性能分析结果。进一步,运用锥补线性化技术提出优化控制设计算法,能够获得最小的H∞性能指标并实现控制器增益和观测器增益的协同设计。通过仿真结果验证,所提出的自抗扰采样控制方法通过前轮和后轮转向使得车辆的侧偏角和偏航角速度能够在数据采样环境下跟踪期望目标,而经典的基于干扰观测器的控制方法无法完成跟踪控制任务;所提控制方法相比于经典的比例控制降低95%的跟踪误差。

关键词：四轮转向自抗扰控制采样控制鲁棒控制

西藏高寒地区冷季圈舍环境参数及其与舍饲牦牛增重的关系

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中国草地学报 2024年第4期46卷 112-119页

作者：孙梦娇王梓凡孙光明洛桑顿珠次旦央吉朱彦宾巴桑旺堆张晓庆青岛农业大学草业学院山东青岛266109 中国农业科学院草原研究所内蒙古呼和浩特010010 西藏自治区农牧科学院畜牧兽医研究所西藏拉萨850000 西藏自治区农牧科学院草业科学研究所西藏拉萨850000

为深入了解西藏高寒地区冷季圈舍环境参数及其与舍饲牦牛增重的关系,本研究在拉萨市的林周县、当雄县和尼木县典型牦牛养殖场,分别随机挑选20头牦牛于1~3月进行为期79 d的全舍饲饲养,并监测牛舍内温度、湿度及CO_(2)、PM2.5和PM10浓度... 详细信息

为深入了解西藏高寒地区冷季圈舍环境参数及其与舍饲牦牛增重的关系,本研究在拉萨市的林周县、当雄县和尼木县典型牦牛养殖场,分别随机挑选20头牦牛于1~3月进行为期79 d的全舍饲饲养,并监测牛舍内温度、湿度及CO_(2)、PM2.5和PM10浓度等环境参数和牦牛日增重。结果表明:试验期内,3县牛舍内温度、湿度差异显著,尼木县牛舍内平均温度和湿度最高,分别为3.96℃和38.19%,林周县分别为1.50℃和25.33%,当雄县分别为-3.83℃和36.21%;尼木县牛舍内CO_(2)浓度最高(961.19 mg/m^(3)),当雄县次之(879.35 mg/m^(3)),林周县最低(837.65mg/m^(3));PM2.5和PM10浓度为林周县最高,尼木县次之,当雄县最低。各县牛舍内1~3月温度呈上升趋势,其他环境参数变化趋势不一致。各县牦牛日增重存在显著差异,其中尼木县最高(587.91g/d),当雄县次之(321.53g/d),林周县最低(262.14 g/d)。回归分析表明,牦牛日增重与牛舍内温度、CO_(2)浓度存在正相关关系,与湿度、PM2.5和PM10浓度无直接关系。由以上结果可见,在冷季漫长的西藏高寒地区改善牛舍环境有益于提高牦牛生产性能。

关键词：高寒地区牦牛畜舍环境日增重 CO_(2)排放