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大豆铝激活苹果酸转运家族基因GmALMT8的鉴定与功能验证

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植物遗传资源学报 2024年第6期25卷 1027-1034页

作者：刘慧许文静张红梅刘晓庆张威王琼朱月林陈华涛江苏省农业科学院经济作物研究所南京210014 南京农业大学园艺学院南京210095

鲜食风味是影响菜用大豆食味品质的关键因素,其形成与有机酸有着密切的关联,研究有机酸合成机制对于菜用大豆的品质改良具有重要的实际意义。本研究利用大豆毛状根系统,探究与苹果酸含量显著相关的候选基因GmALMT8、GmIF7GT5和GmAP在调... 详细信息

鲜食风味是影响菜用大豆食味品质的关键因素,其形成与有机酸有着密切的关联,研究有机酸合成机制对于菜用大豆的品质改良具有重要的实际意义。本研究利用大豆毛状根系统,探究与苹果酸含量显著相关的候选基因GmALMT8、GmIF7GT5和GmAP在调控苹果酸含量方面的功能,结果表明:在GmALMT8-OE毛状根中,GmALMT8基因表达量与苹果酸的含量均显著高于空载对照毛状根,而GmIF7GT5和GmAP毛状根中苹果酸的含量无显著变化。鉴于已经报道的ALMT家族基因的苹果酸转运功能,推测大豆中GmALMT8基因可能具有相似的功能,在调控苹果酸含量方面发挥重要作用。为验证GmALMT8-OE毛状根中苹果酸含量的变化是否由GmALMT8基因表达的改变所引起,本研究采用蘸花法在拟南芥中过表达GmALMT8基因。与阳性毛状根中苹果酸测定的结果相类似,过表达GmALMT8显著提高了T2.转基因拟南芥株系种子中的苹果酸含量,进一步证明GmALMT8的稳定表达能够提高苹果酸含量,明确GmALMT8基因在大豆中具有调控苹果酸含量的生物学功能,丰富了大豆有机酸的理论研究,对菜用大豆优质育种具有参考价值。

关键词：菜用大豆苹果酸候选基因功能分析

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Selectivity switching between CO and formate for CO_(2. reduction on Sb modified amorphous ZnO by electronic effect

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Nano Research 2023年第10期16卷 12144-12152页

作者： Hongyu Chen Shoufu Cao Lu wang Xiaojing Lin Qiuying zhu Yizhu Shang Shuxian Wei Siyuan Liu Zhaojie wang Baojun Wei xiaoqing Lu College of Science China University of PetroleumQingdao 266580China School of Materials Science and Engineering China University of PetroleumQingdao 266580China

The adjustable intermediate binding capacity in electrocatalytic carbon dioxide(CO_(2.)reduction is critical for varying the reaction pathways to desired ***,we first report the synthesis of boron-doped amorphous zinc oxide with(B-a-ZnO-Sb)or without antimony nanoparticles embedding(B-a-ZnO)via one-step wet chemical method,which is easy to scale up by enlarging the vessel and increasing *** successfully realizes the product switching from CO on B-a-ZnO to formate on *** experimental and theoretical results reveal that Sb weakens the charge interaction on Zn *** on the moderate adsorption of*COOH and strong adsorption of*OCHO and*HCOOH for B-a-ZnO,the foreign Sb weakens the adsorption of these intermediates and brings about a favor formate production instead of *** work points out a new direction for the synthesis of amorphous ZnO-based catalysts and provides advanced insights into the aimed selectivity switch for CO_(2.reduction by electronic effect.

关键词： carbon dioxide reduction amorphous ZnO electronic effect selectivity switching

Meteorological influences on co-occurrence of O_(3) and PM_(2.5)pollution and implication for emission reductions in Beijing-Tianjin-Hebei

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Science china Earth sciences 2023年第6期66卷 1258-1267页

作者： xiaoqing MA Zhicong YIN Bufan CAO Huijun wang key laboratory of Meteorological Disaster Ministry of Education/Joint International Research Laboratory of Climate and Environment Change(ILCEC)/Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters(CIC-FEMD)Nanjing University of Information Science&TechnologyNanjing 210044China Southern Marine Science and Engineering Guangdong laboratory(zhuhai) Zhuhai 519080China Nansen-zhu International Research Centre Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China

Co-occurrence of surface ozone(O_(3))and fine particulate matter(PM_(2.5))pollution(CP)was frequently observed in Beijing-Tianjin-Hebei(BTH).More than 50%of CP days occurred during April-May in BTH,and the CP days reached up to 11 in two months of *** PM_(2.5)or O_(3) concentration associated with CP was lower than but close to that in O_(3) and PM_(2.5)pollution,indicating compound harms during CP days with double-high concentrations of PM_(2.5)and O_(3).CP days were significantly facilitated by joint effects of the Rossby wave train that consisted of two centers associated with the Scandinavia pattern and one center over North china as well as a hot,wet,and stagnant environmental condition in *** 2.18,the number of CP days decreased sharply while the meteorological conditions did not change ***,changes in meteorological conditions did not really contribute to the decline of CP days in 2.19 and *** implies that the reduction of PM_(2.5)emission has resulted in a reduction of CP days(about 11 days in 2.19 and 2.2.).The differences in atmospheric conditions revealed here were helpful to forecast the types of air pollution on a daily to weekly time *** reduction in PM_(2.5)emission was the main driving factor behind the absence of CP days in 2.2.,but the control of surface O_(3) must be stricter and deeper.

关键词： Co-occurrence pollution Ozone PM_(2.5) Meteorology Emission reduction

乳酸菌的乙醇耐受机制及其在食醋生产中的应用

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现代食品科技 2024年第3期40卷 91-101页

作者：张霖夏程程王文悦余帆肖柯易弛樊鑫朱晓青肖俊锋李琴汪超穆杨周梦舟湖北工业大学发酵工程教育部重点实验室工业发酵省部协同创新中心湖北省食品发酵工程技术研究中心湖北武汉430068

传统固态发酵食醋因其丰富的菌群在风味、品质等方面有着不可比拟的优势,乳酸菌作为固态食醋发酵中重要的功能微生物,广泛应用于食品、生物等领域。然而,食醋在发酵过程中乳酸菌会受到高乙醇环境的胁迫,因此探究乳酸菌如何在高乙醇环境... 详细信息

传统固态发酵食醋因其丰富的菌群在风味、品质等方面有着不可比拟的优势,乳酸菌作为固态食醋发酵中重要的功能微生物,广泛应用于食品、生物等领域。然而,食醋在发酵过程中乳酸菌会受到高乙醇环境的胁迫,因此探究乳酸菌如何在高乙醇环境下生存具有积极意义。该研究选择耐乙醇副干酪乳杆菌(PC-5)和不耐乙醇植物乳杆菌(PR-7)作为实验对比菌株,通过检测生物指标发现:在体积分数为8%的乙醇环境下,PC-5的多糖含量占比和细胞膜通透性分别提高至0.56%和75%,且显著高于PR-7。此外,代谢途径中的己糖激酶(Hexokinase,HK)、6-磷酸果糖激酶(6-Phosphofructokinase,PFK)、丙酮酸激酶(Pyruvate Kinase,PK)的活性均高于PR-7,并分别提高至99.82.2.78、3.43 U/mg。最后,在食醋不同发酵阶段中加入PC-5使其与酵母菌实现共同发酵,结果发现:共发酵食醋体系中多酚的生成和总抗氧化能力比单菌发酵分别提升了32.14%和55.56%。因此乳酸菌对食醋发酵有着良好的促进作用,为乳酸菌参与食醋共发酵提供了良好的理论依据。

关键词：乳酸菌细胞膜乙醇胁迫混菌发酵

OPTIMIZATION ON ANTENNA PATTERN OF SPACEBORNE SAR WITH IMPROVED NSGA-Ⅱ

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Journal of Electronics(china) 2009年第4期26卷 443-447页

作者： Xiao Jiang wang xiaoqing {2. Minhui Xiao Liu National key laboratory of Microwave Imaging Technology Beijing 100190 China institute of Electronics Chinese Academy of Sciences Beijing 100190 China

Optimization of antenna array pattern used in a spaceborne Synthetic Aperture Radar (SAR) system is considered in this study. A robust evolutionary algorithm, Non-dominated Sorting Genetic Algorithms (the improved NSGA-Ⅱ), is applied on a spaceborne SAR antenna pattern design. The system consists of two objective functions with two constraints. Pareto fronts are generated as a result of multi-objective optimization. After being validated by a test problem ZDT4, the algorithms are used to synthesize spaceborne SAR antenna radiation pattern. The good results with low Ambi- guity-to-Signal Ratio (ASR) and high directivity are obtained in the paper.

关键词： Synthetic Aperture Radar (SAR) Radiation pattern Improved Non-dominated Sorting Genetic Algorithms (NSGA)-Ⅱ Ambiguity-to-Signal Ratio (ASR)

南黄海SYS90-1A孔第四纪沉积物磁性地层学研究

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海洋地质与第四纪地质 2024年第2期44卷 97-109页

作者：陆凯孙军李广雪杨慧良王忠蕾梅西陈晓辉祁江豪朱晓青强小科中国海洋大学海洋地球科学学院青岛266100 中国地质调查局青岛海洋地质研究所青岛266237 崂山实验室海洋矿产资源评价与探测技术功能实验室青岛266237 中国地质大学(北京)海洋学院北京100083 黄土与第四纪地质国家重点实验室中国科学院地球环境研究所西安210008

可靠的年代地层框架是开展南黄海地区第四纪环境演变研究的基础。目前南黄海南部地区缺乏可靠年代学控制的长序列钻孔。通过对南黄海南部SYS90-1A孔(孔深90.1 m)沉积物开展详细的磁性地层学研究,并结合AMS14C测年结果及区域已有磁性地... 详细信息

可靠的年代地层框架是开展南黄海地区第四纪环境演变研究的基础。目前南黄海南部地区缺乏可靠年代学控制的长序列钻孔。通过对南黄海南部SYS90-1A孔(孔深90.1 m)沉积物开展详细的磁性地层学研究,并结合AMS14C测年结果及区域已有磁性地层学结果,建立南黄海南部地区第四纪沉积物年代地层框架。结果显示,SYS90-1A孔岩芯记录了从布容(Brunhes)正极性时至松山(Matuyama)负极性时上部,包括Kamikatsura和Santa Rosa地磁漂移事件。早、中更新世界线即布容正极性时与松山负极性时界线(B/M界线)深度位于74.2.m,Kamikatsura和Santa Rosa地磁漂移事件深度分别位于79.75~82.47 m和85.2.~87.74 m。根据Kamikatsura和Santa Rosa地磁漂移事件的年代控制点和平均沉积速率,推算钻孔底部年龄为0.96 Ma。南黄海SYS90-1A孔早更新世晚期的沉积速率约为8.66 cm/ka,中更新世以来的沉积速率约为9.5 cm/ka,而全新世以来的沉积速率为12.8 cm/ka,沉积速率自早更新世晚期以来呈增加趋势。这一结果不仅为南黄海南部第四纪沉积物研究提供了有效的年代学约束,而且为南黄海地区第四纪地层划分和对比、沉积环境与气候变化、物源示踪等研究提供了重要的时间标尺。

关键词：古地磁学沉积速率第四纪中部泥质区南黄海

Hierarchical recognition on the taxonomy of Nitzschia closterium f. minutissima

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chinese Science Bulletin 2008年第2期53卷 245-250页

作者： SHI Juan PAN KeHou wang xiaoqing CHEN Fang ZHOU Mi {2. BaoHua QING RenWei key laboratory of Mariculture of Ministry of Education Ocean University of China Qingdao 266003 China key laboratory of Bioresources and Eco-environment of Ministry of Education School of Life Sciences Sichuan University Chengdu 610064 China

Nitzschia closterium f. minutissima, a marine eukaryotic unicellular diatom, originally classified as Bacillariophyta/Bacillariophyceae/Bacillariales/Bacillariaceae/Nitzschia, is one of the most important feed sources in mariculture. In this study, its morphological features were examined under DIC Microscopy (differential interference contrast microscope); its pigments and fatty acids composition were analyzed by using High Performance Liquid Chromatography (HPLC) and gas chromatography (GC); the complete Actin cDNA, part 18S rDNA, complete ITS1 and ITS2.sequences, part 2.S rDNA se- quences, and a putatively encoding ?5 fatty acid desaturase gene were cloned respectively and further functioned in transgenic yeast. The sequence alignments were separately conducted using the related sequences from Nitzschia closterium f. minutissima, Cylindrotheca closterium (Bacillariophyta/Baci- llariales/Bacillariaceae/Cylindrotheca) and Phaeodactylum tricornutum (Naviculales) with ClustalX 1.83. No distinct difference was discovered between N. closterium f. minutissima and P. tricornutum in both biochemical and molecular level. Their identity was more than 99.6% among 18S rDNA, 5.8S rDNA and actin-gene sequences, and is up to 98.6% even among ITS1 and ITS2.sequences. Their ?5 desaturase similarity was 99.4%. However, the lower similarity was disclosured between N. closterium f. minutis- sima and Cylindrotheca closterium, which shared less than 40% identity in the ITS1 and ITS2.se- quences. So, N. closterium f. minutissima should not be placed in Bacillariales, Bacillariaceae, Nitzschia, but in Naviculales, Phaeodactylaceae, Phaeodactylum, and it was actually a strain of P. tri- cornutum.

关键词：单细胞硅藻类真核状态食物源海产养殖

A step-by-step multiple stimuli-responsive metal-phenolic network prodrug nanoparticles for chemotherapy

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Nano Research 2022年第2期15卷 1205-1212页

作者： xiaoqing Yi Weijia Zeng Cui wang Ying Chen Liangyuan Zheng Xinlin {2. Yuqiu Ke Xiaoyan He Ying Kuang Qitong Huang key laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases Ministry of EducationKey Laboratory of Biomaterials and Biofabrication in Tissue Engineering of Jiangxi ProvinceGannan Medical UniversityGanzhou 341000China Faculty of Materials Metallurgy and Chemistry Jiangxi University of Science and TechnologyGanzhou 341000China School of Life sciences Anhui Medical UniversityHefei 230032China

Currently,chemotherapy is the main clinical therapy of ***,most chemotherapeutic drugs such as doxorubicin and paclitaxel(PTX)have poor water solubility,leading to low bioavailability and serious side *** now,although a variety of nanoparticulate drug delivery systems have been designed to ameliorate the above disadvantage of chemotherapy drugs,their application is still severely limited due to the complex preparation,poor stability,low drug loading,and premature drug ***,a metal phenolic network-based drug delivery system with superior stability,satisfactory drug loading capacity,good biocompatibility,reduced undesired premature release,and excellent anti-tumor ability has been established for achieving step-by-step multiple stimuli-responsive drug ***,the redox-responsive dimeric paclitaxel(diPTX)prodrug was *** diPTX@Fe&tannic acid(diPTX@Fe&TA)complex nanoparticles with satisfactory PTX loading capacity were obtained by deposition of Fe&TA network complex on the nanocore of diPTX rapidly with a simple *** diPTX@Fe&TA nanoparticles have a hydrodynamic diameter of 152.6±1.2.nm,long-term colloidal stability,and high PTX loading content of 2..7%.Besides,diPTX@Fe&TA could expose to the acidic lysosomal environment and the reduction cytoplasmic environment continuously,resulting in the sequential release of diPTX and PTX when it was phagocytosed by tumor ***,PTX showed almost no release under physiological condition(pH 7.4),which effectively inhibited the undesirable premature release of *** importantly,diPTX@Fe&TA could suppress the growth of tumor effectively in vivo,along with negligible toxicity for *** work developed a simple and novel approach for the construction of a stepwise multiple stimuli-responsive drug delivery system with superior stability and satisfactory drug loading capacity to inhibit tumor growth effectively.

关键词： drug delivery step-by-step multiple stimuli-responsive metal-phenolic network superior stability

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新疆后造山碱性花岗岩的地质特征

岩石学报 2006年第12期22卷 2945-2956页

作者：朱笑青王中刚王元龙毕华中国科学院地球化学研究所矿床地球化学国家重点实验室贵阳550002 中国科学院地质与地球物理研究所北京100029 海南师范学院海口571158

新疆地区碱性花岗岩分布很广泛,可以划分出十条岩带,它们主要分布在古生代岛弧、弧后盆地及隆起区,与板块缝合线、深断裂密切相关,并与蛇绿岩和陆相火山岩相伴生。同位素年代测定表明,本区碱性花岗岩的形成是紧随造山运动之后发生的,属... 详细信息

新疆地区碱性花岗岩分布很广泛,可以划分出十条岩带,它们主要分布在古生代岛弧、弧后盆地及隆起区,与板块缝合线、深断裂密切相关,并与蛇绿岩和陆相火山岩相伴生。同位素年代测定表明,本区碱性花岗岩的形成是紧随造山运动之后发生的,属于后造山花岗岩。因此,它们形成时的构造环境、岩石化学成分、矿物组成及微量元素特征等均与非造山花岗岩(A 型花岗岩)有一定的区别。在一个地区有这么多后造山碱性花岗岩分布,国内外并不多见,这是新疆地质的一个特色。

关键词：碱性花岗岩造山运动岩石学新疆