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Frontiers of Materials Science 2015年第1期9卷 51-65页

作者： Peng WANG Bin PI Jin-Ning WANG Xue-song zhu Hui-lin YANG Department of Orthopedic Surgery the First Affiliated Hospital of Soochow University Suzhou 215006 China

To search for new bioactive materials which can be used as the substitute of bone repairing and drug carriers, Sema3A-Ioaded chitosan microspheres （SLCM） and silk fibroin （SF） were mixed with calcium sulfate cement （CSC）. SEM, particle size analysis and swelling rate determination were performed to study properties of the microspheres. The drug loading, encapsulation efficiency and drug release rate were determined by ELISA. Microspheres with different SLCM weight contents （0.5%, 1% and 5%） were prepared to determine which one has the strongest mechanical properties and the appropriate setting time. It was revealed that CSC/SF/0.5SLCM has satisfactory mechanical properties, and its in vitro biocompatibility was assessed by MTS. Chitosan microspheres （5-18 μm） were globular, the surface was smooth, and the swelling rate is （77.02 ± 5.57）%. With this formula, the setting time was increased with the addition of SLCM in CSCISF, and the cumulative drug release rate is 44.62% in 28 d. XRD results demonstrate that the main component is calcium sulfate. Also it was found that CSCiSFI 0.5SLCM supports the growth of MC3T3 cells. Thus the preparation of CSCISFIO.5SLCM was reliable, and the products had good structures, physical properties and biocompatibility, appearing to be a promising bone substitute material.

关键词： Sema3A chitosan microsphere sustained release silk fibroin (SF) bone cement

Characteristics of nucleotides and amino acids in cDNA sequence of xyloglucan endotransglycosylase cloned from Anthocephalus chinensis

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Forestry Studies in China 2011年第1期13卷 45-51页

作者： LI Wei zhu song-lin LI Na CHEN Xiao-yang Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants of Ministry of Education National Engineer- ing Laboratory for Forest Tree Breeding College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 P. R. China State-owned Forest Administration of Lvliangshan Mountain Linfen 041000 P. R. China College of Forestry South China Agricultural University Guangzhou 510642 E R. China

Xyloglucan endotransglycosylase is an identified relaxation factor with functions of easing and extending of plant cell *** activities are directly related to plant growth and elongation of *** chinensis is a tall and fast growing evergreen tree *** cloned the full cDNA sequence of AcEXT genes which is abundantly expressed in the cambium of *** sequence analysis of nucleotides and amino acids revealed the presence of a 1396 bp full cDNA sequence,including a 960 bp complete open reading frame（ORF） encoding a 320 amino acid *** deduced amino acid sequence of AcXET was homologous to the other known XET proteins and contained the conserved EIDFE catalytic site which was specific to all the *** data should serve as a foundation for further insight into AcXET gene molecular mechanisms during wood formation and cell wall engineering of woody plants.

关键词： cloning XET gene sequence analysis Anthocephalus chinensis

Cloning of XET gene from Anthocephalus chinensis and its plant expression vector construction

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Forestry Studies in China 2010年第2期12卷 79-84页

作者： MA Sheng-jun zhu song-lin LI Wei OUYANG Kun-xi LI Na CHEN Xiao-yang Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education National Engineering Laboratory for Forest Tree Breeding Beijing Forestry University Beijing 100083 P. R. China State-owned Forest Administration of Lvliangshan Mountain Linfen 041000 P. R. China Forestry Research Institute of Hebei Province Shijiazhuang 050061 P. R. China College of Forestry South China Agricultural University Guangzhou 200064 P. R. China

A full-length cDNA sequence of xyloglucan endotransglycosylase gene （XET）, abundantly expressed in the cambium of Anthocephalus chinensis was cloned by conserved PCR, rapid-amplification of cDNA ends and by chromosome walking. Analytical results of the DNA sequence show that a 912 bp complete open reading frame （ORF） encoded a 303-amino acid protein was in the 1205 bp full cDNA sequence. The deduced amino acid sequence of AcXET, which contained the conserved specific EIDFE catalytic site sequence to XETs was homologous to the other known XET proteins. In order to study the gene function of AcXET and obtain transgenic plants, a plant expression vector pBIAcXET was constructed by recombinating the AcXET fragment from the cloning vector pMD19AcXET and the binary vector pBI121 between the XbaI and SmaI sites. The fragment ofAcXET gene was inserted between the CaMV 35S promotor and the coding region of the GUS gene in pBI121. The identification results show that the plant expression binary vector pBIAcXET was constructed successfully. These results lay the foundation for studying the molecular mechanism ofAcXET gene during wood formation.

关键词： cDNA cloning sequence analysis AcXET gene plant expression vector

Assessments of Data-Driven Deep Learning Models on One-Month Predictions of Pan-Arctic Sea Ice Thickness

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Advances in Atmospheric Sciences 2024年第7期41卷 1379-1390页

作者： Chentao song Jiang zhu Xichen LI Institute of Atmospheric Physics Chinese Academy of SciencesBeijing 100029China University of Chinese Academy of Sciences Beijing 100049China

In recent years,deep learning methods have gradually been applied to prediction tasks related to Arctic sea ice concentration,but relatively little research has been conducted for larger spatial and temporal scales,mainly due to the limited time coverage of observations and reanalysis ***,deep learning predictions of sea ice thickness(SIT)have yet to receive ample *** this study,two data-driven deep learning(DL)models are built based on the ConvLSTM and fully convolutional U-net(FC-Unet)algorithms and trained using CMIP6 historical simulations for transfer learning and fine-tuned using reanalysis/*** models enable monthly predictions of Arctic SIT without considering the complex physical processes *** comprehensive assessments of prediction skills by season and region,the results suggest that using a broader set of CMIP6 data for transfer learning,as well as incorporating multiple climate variables as predictors,contribute to better prediction results,although both DL models can effectively predict the spatiotemporal features of SIT *** the predicted SIT anomalies of the FC-Unet model,the spatial correlations with reanalysis reach an average level of 89%over all months,while the temporal anomaly correlation coefficients are close to unity in most *** models also demonstrate robust performances in predicting SIT and SIE during extreme *** effectiveness and reliability of the proposed deep transfer learning models in predicting Arctic SIT can facilitate more accurate pan-Arctic predictions,aiding climate change research and real-time business applications.

关键词： Arctic sea ice thickness deep learning spatiotemporal sequence prediction transfer learning

A two-field-scan harmonic Hall voltage analysis for fast, accurate quantification of spin-orbit torques in magnetic heterostructures

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Science China(Physics,Mechanics & Astronomy) 2024年第10期Mechanics & Astronomy) .卷 109-116页

作者： Xin lin Lijun zhu State Key Laboratory of Superlattices and Microstructures Institute of SemiconductorsChinese Academy of Sciences College of Materials Science and Opto-Electronic Technology University of Chinese Academy of Sciences

We report on the development of a “two-field-scan” harmonic Hall voltage(HHV) analysis, which collects the second HHVas a function of a swept in-plane magnetic field at 45° and 0° relative to the excitation current, for the determination of the spin-orbit torques of transverse spins in magnetic heterostructures without significant perpendicular spins, longitudinal spins, and longitudinal/perpendicular Oersted fields. We demonstrate that this two-field-scan analysis is as accurate as the well-established but time-consuming angle-scan HHV analysis even in the presence of considerable thermoelectric effects but takes more than a factor of 7 less measurement time. We also show that the fit of the HHV data from a single field scan at 0°, which is commonly employed in the literature, is not reliable because the employment of too many free parameters in the fitting of the very slowly varying HHV signal allows erroneous conclusion about the spin-orbit torque efficiencies.

关键词： spin-orbit torque spin current spin-orbit coupling spintronics

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Adaptive nonlinear Kalman filters based on credibility theory with noise correlation

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Chinese Journal of Aeronautics 2024年第6期37卷 232-243页

作者： Quanbo GE Zihao song Bingtao zhu Bingjun ZHANG School of Automation Nanjing University of Information Science and TechnologyNanjing 210044China Jiangsu Collaborative Innovation Center on Atmospheric Environment and Equipment Technology Nanjing 210044China School of Electrical and Automation Engineering East China Jiaotong UniversityNanchang 330013China School of Logistics Engineering Shanghai Maritime UniversityShanghai 201306China School of Electronic and Information Engineering Tongji UniversityShanghai 200092China

To solve the divergence problem and overcome the difficulty in guaranteeing filtering accuracy during estimation of the process noise covariance or the measurement noise covariance with traditional new information-based nonlinear filtering methods,we design a new method for estimating noise statistical characteristics of nonlinear systems based on the credibility Kalman Filter(KF)theory considering noise *** method first extends credibility to the Unscented Kalman Filter(UKF)and Extended Kalman Filter(EKF)based on the credibility ***,an optimization model for nonlinear credibility under noise related conditions is established considering noise correlation.A combination of filtering smoothing and credibility iteration formula is used to improve the real-time performance of the nonlinear adaptive credibility KF algorithm,further expanding its application scenarios,and the derivation process of the formula theory is ***,the performance of the nonlinear credibility filtering algorithm is simulated and analyzed from multiple perspectives,and a comparative analysis conducted on specific experimental *** simulation and experimental results show that the proposed credibility EKF and credibility UKF algorithms can estimate the noise covariance more accurately and effectively with lower average estimation time than traditional methods,indicating that the proposed algorithm has stable estimation performance and good real-time performance.

关键词： Kalman filter Extended Kalman Filter(EKF) Unscented Kalman Filter(UKF) Credibility Noise correlation

Regulation and application of supramolecular gel with circularly polarized luminescence

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Science China Chemistry 2024年第6期67卷 1865-1880页

作者： Shen song Yulong Shi Liangliang zhu Bingbing Yue School of Materials and Chemistry&Institute of Bismuth and Rhenium University of Shanghai for Science and TechnologyShanghai200093China State Key Laboratory of Molecular Engineering of Polymers Fudan UniversityShanghai200438China

Supermolecular gel is a three-dimensional network structure assembled by small molecules or polymers in solvents through noncovalent *** emergence of system complexity occurs spontaneously during the molecular self-assembly process.A multitude of chiral molecular self-assembly systems have been engineered,facilitating the achievement of circularly polarized luminescence(CPL)through the amalgamation of chiral entities with *** bonding,π-πstacking,and noncovalent forces,such as host-guest interactions and Van der Waals'forces,confer upon supramolecular gels the capacity to react to diverse *** to the flexibility of supramolecular assembly,the CPL properties of supramolecular gels have rich controllability and can be used in various *** this review,we summarized the examples of CPL-active supramolecular gel assembly,and further summarized the assembly environment factors and external ***,the versatility of CPL applications in supramolecular gels is demonstrated,ranging from optical devices,information encryption,biosensing and chemical sensing,and other practical *** conclusion,the study provides insights into the multicultural factors influencing CPL in supramolecular gels,describes their applications in various domains,and presents future perspectives in the field.

关键词： supramolecular gels circularly polarized luminescence self-assembly chirality stimuli-response

Global practical stabilization of the double integrator system with an imperfect sensor and subject to a bounded disturbance

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Control Theory and Technology 2024年第3期22卷 468-478页

作者： Zhiyuan zhu Yuanlong Li Zongli lin Department of Automation Shanghai Jiao Tong Universityand Key Laboratory of System Control and Information Processing of Ministry of EducationShanghai 200240China Charles L.Brown Department of Electrical and Computer Engineering University of VirginiaP.0.Box 400743CharlottesvilleVA 22904-4743USA

This paper is concerned with global practical stabilization of the double integrator system with an imperfect sensor and subject to an additive bounded output *** imperfect sensor nonlinearity possesses the nonlinear characteristics of saturation and dead *** of the presence of output dead zone and the additive disturbance,the states cannot be expected to driven into an arbitrarily small neighborhood of the *** solve the global practical stabilization problem,we proposes a low gain-based linear dynamic output feedback law,under which the first state enters and remains in a bounded set whose size is depended on the bound of disturbance and the range of dead zone and the second state enters and remains in a pre-specified arbitrarily small set,both in finite *** results illustrate the effectiveness of our proposed control method.

关键词： Output saturation Disturbance rejection Output dead zone Low gain feedback-linear output feedback Practical stabilization

NFXL1 functions as a transcriptional activator required for thermotolerance at reproductive stage in Arabidopsis

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Journal of Integrative Plant Biology 2024年第1期66卷 54-65页

作者： Qiao‐Yun zhu lin‐lin Zhang Jian‐Xiang Liu State Key Laboratory of Plant Environmental Resilience College of Life SciencesZhejiang UniversityHangzhou 310027China

Plants are highly susceptible to abiotic stresses,particularly heat stress during the reproductive ***,the specific molecular mechanisms underlying this sensitivity remain largely *** the current study,we demonstrate that the Nuclear Transcription Factor,X-box Binding Protein 1-Like 1(NFXL1),directly regulates the expression of DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN 2A(DREB2A),which is crucial for reproductive thermotolerance in ***1 is upregulated by heat stress,and its mutation leads to a reduction in silique length(seed number)under heat stress ***-Seq analysis reveals that NFXL1 has a global impact on the expression of heat stress responsive genes,including DREB2A,Heat Shock Factor A3(HSFA3)and Heat Shock Protein 17.6(HSP17.6)in flower ***,NFXL1 is enriched in the promoter region of DREB2A,but not of either HSFA3 or *** experiments using electrophoretic mobility shift assay have confirmed that NFXL1 directly binds to the DNA fragment derived from the DREB2A ***,effector-reporter assays have shown that NFXL1 activates the DREB2A *** DREB2A mutants are also heat stress sensitive at the reproductive stage,and DEREB2A is epistatic to NFXL1 in regulating thermotolerance in flower *** is known that HSFA3,a direct target of DREB2A,regulates the expression of heat shock proteins genes under heat stress ***,our findings establish NFXL1 as a critical upstream regulator of DREB2A in the transcriptional cassette responsible for heat stress responses required for reproductive thermotolerance in Arabidopsis.

关键词： Arabidopsis DREB2A heat stress NFXL1 reproductive thermotolerance

linguistic landscape and its impacts on place-making in mountain destination:A case of Mogan Mountain Town of Huzhou City,China

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Journal of Mountain Science 2024年第5期21卷 1613-1629页

作者： LI Chenyan LU song LONG Cheng zhu Jiaheng School of Tourism Shanghai Normal UniversityShanghai 200034China School of Environmental and Geographical Sciences Shanghai Normal UniversityShanghai 200034China

The place and placeness are believed to be a crucial perspective for comprehending the transformative dynamics of tourist destinations in the context of globalization and the rapid development of the tourism *** the material carrier of language,linguistic landscape is an intermediary for people to endow emotions and personalities into the place,becoming an effective path for exploring the place-making in a certain *** Mountain is a famous national tourist resort,which is located in Mogan Mountain Town,Deqing County,Huzhou City,*** is a good case area for studying the relationship between linguistic landscape and placemaking in tourist *** are two theories that form the theoretical foundation of the study,namely the place and the ***,the results showed the overall style of the linguistic *** labelling(52.7%),official signs(55.3%),and standardized Chinese characters predominantly shaped the linguistic landscape,with English and other languages being ***,from the perspective of three dimensions of the geosemiotics framework,such as inscription,language preference,and placement position,we conduct a comprehensive semiotic analysis of the linguistic landscape,which encompasses diverse facets such as fonts,materials,shifts in state,text vectors,and symmetrical ***,we explore the influence of linguistic landscapes on the place-making of Mogan Mountain through the dimensions of location,locale,and sense of *** information on linguistic landscapes indicates the geographical *** linguistic landscapes play an important role in constructing the language order,nostalgic atmosphere,exotic atmosphere,and elegant and tasteful lifestyle of the public space in Mogan *** of linguistic landscapes,including tourists and residents,generate and identify with the unique sense of place in Mogan *** linguistic landscapes construct the destinati

关键词： linguistic landscape Place-making Tourist destination Geosemiotics Place theory Place branding Mogan Mountain Town China