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5DFRXXL region of long myosin light chain kinase causes F-actin bundle formation

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Chinese Science Bulletin 2005年第18期50卷 2044-2050页

作者： YANG Chunxiang WEI Dongmei CHEN Chen YU Weiping zhu minsheng Model Animal Research Center Nanjing University Nanjing 210061 China Medical College Southeast University Nanjing 210009 China Nanjing Normal University Nanjing 210097 China

Long myosin light chain kinase (L-MLCK) contains five DFRXXL motifs with ability to bind F-actin. Binding stoichiometry data indicated that each DFRXXL motif might bind each G-actin, but its biological significance remained unknown. We hypothesized that L-MLCK might act as an F-actin bundle peptides by its multiple binding sites of 5DFRXXL motifs to actin. In order to characterize F-actin-bundle formation properties of 5DFRXXL region of long myosin light chain kinase, we expressed and purified 5DFRXXL peptides tagged with HA in vitro. The properties of 5DFRXXL peptides binding to myofilaments or F-actin were analyzed by binding stoichiometries assays. The results indicated that 5DFRXXL peptides bound to myofilaments or F-actin with high affinity. KD values of 5DFRXXL binding to myofilaments and F-actin were 0.45 and 0.41 μmol/L, re- spectively. Cross-linking assay demonstrated that 5DFRXXL peptides could bundle F-actin efficiently. Typical F-actin bundles were observed morphologically through determina- tion of confocal and electron microscopy after adding 5DFRXXL peptides. After transfection of pEGFP-5DFRXXL plasmid into eukaryocyte, spike structure was observed around cell membrane edge. We guess that such structure formation may be attributable to F-actin over-bundle forma- tion caused by 5DFRXXL peptides. Therefore, we suppose that L-MLCK may be a new bundling protein and somehow play a certain role in organization of cell skeleton besides mediating cell contraction by it kinase activity.

关键词：肌浆球蛋白链致活酶肌丝 DFRXXL 生物学

Abnormal expression of inducible nitric oxide synthase in Duchenne muscular dystrophy muscles

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Chinese Science Bulletin 1998年第10期43卷 860-863页

作者： minsheng zhu Yuhua Fan Xiangyu Xu Gang Wang Yue Shen Ying Pan Shubiao Ding Nanjing Mil Inst Med Sci Nanjing 210002 Peoples R China Nanjing Children Hosp Dept Neurol Nanjing 210005 Peoples R China

Duchenne muscular dystrophy (DMD) is a fatal genetic disease for the youth and children. 8 biopsies of DMD patients were determined and demonstrated that the membrane_binding nitric oxide synthase was enriched in normal skeletal muscles and was little in DMD muscles. The results from Western blot and immunohistochemistry showed that inducible nitric oxide synthase (iNOS) was overexpressed in DMD muscle fibers, while a small amount of highly localized iNOS can be found in normal fibers. Based on these findings, it is proposed that the mechanism of progressive injury in DMD muscle might be associated with the abnormal expression of iNOS.

关键词： Duchenne muscular dystrophy inducible nitric oxide synthase human.

Comparative Immunization in BALB/c Mice with Recombinant Replication-Defective Adenovirus Vector and DNA Plasmid Expressing a SARS-CoV Nucleocapsid Protein Gene

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Cellular & Molecular Immunology 2006年第6期3卷 459-465页

作者： Chunling Ma Kun Yao Feng Zhou minsheng zhu Department of Microbiology and Immunology Nanjing Medical University Nanjing 210029 China Model Animal Research Institute Nanjing University Nanjing 210032 China Department of Microbiology and Immunology Shandong Medical College Linyi 276002 Shandong China

In order to investigate immunogenicity in the induction of humoral and cellular immune responses, severe acute respiratory syndrome associated coronavirus （SARS-CoV）-N gene recombinant replication-defective adenoviral vector, rAd-N, was generated and immunized BALB/c mice in a pcDNA3.1-N prime-rAd-N boost regimen. After humoral and cellular immune response detection, different levels of SARS-CoV N protein specific antibodies and interferon-γ （IFN-γ） secretion are shown compared to controls. The humoral immune response was induced more effectively by the DNA priming and recombinant adenovirus boosting regimen. There is a significant difference between heterogeneous and homologous vaccinations. The heterogeneous combinations were all higher than those of the homologous combinations in the induction of anti-N antibody response. Among the three heterogeneous combinations, pcDNA3.1-N/pcDNA3.1-N/pcDNA3.1-N/rAd-N induced the strongest antibody response. In the induction of IFN-γ production, the homologous combination of rAd-N/rAd-N/rAd-N/rAd-N was significantly stronger than that of pcDNA3.1-N/pcDNA3.1-N/pcDNA3.1-N/pcDNA3.1-N, but was relatively weaker than the heterogeneous combination of pcDAN3.1-N/pcDAN3.1-N/pcDAN3.1-N/rAd-N. This combination was a most efficient immunization regimen in induction of SARS-CoV-N-specific （IFN-γ） secretion just as the antibody response. These results suggest that DNA immunization followed by recombinant adenovirns boosting could be used as a potential SARS-CoV vaccine.

关键词： SARS-CoV DNA vaccine nucleocapsid protein adenovirus vector

Prize-Giving Ceremony for the World Communications Year Held in Beijing

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中国邮政 1984年第1期 31-32页

作者： zhu Xuefan Wen minsheng He Ying Awarding Prizes

The Prize-Giving Ceremony for World Com-munications Year’s (WCY) Photograph andDrawing Competition,Appraisal of Science andTechnology Readings and Literature and Solicita-tion for Art Creation was solemnly held in Na-tional Committee Hall of the *** Beijingon March 16,1984,which was organized by ChinaNational Committee for World CommumcationsYear (CNC WCY).zhu Xuetan,Chairman of theChina National Committee and Vice-Chairman ofthe Standing Committeee,of the National People’s

关键词： World Prize-Giving Ceremony for the World Communications Year Held in Beijing

Preliminary evidence that overexpression of nuclearfactor for IL6 expression (NF-IL6) in NIH3T3 cells may berelated to malignant transformation

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Cell Research 1994年第2期4卷 191-200页

作者： zhu minsheng DINGGAN LIU SHIZUO AKIRA ZAIPING LI.(Shanghai Institute of Biochemistry, Academic Sinica,Shanghai 200031, China)(Institute of Molecular and Cellular Biology, Osaka University, Japan) Institute of Molecular and Cellular Biology Osaka University Japan.

NF-IL6 is a member of c/EBP family and has multiple functions in regulation of cellular gene expression. We have constructed NF-IL6 expression plasmids and trans fected the NIH3T3 cells with them. The sense NF-IL6 transfectants showed significantly increased tumorigenicity, and the stable integration of NF-IL6 cDNA into cellular DNA and its expression were demonstrated. Our results suggest that NF-IL6 may be related to tumorigenesis.

关键词： NF-IL6 NIH3T3 tumorigenicity

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用于基因频率计算的常规方法和金榜方法

生物学教学 2009年第10期34卷 41-42页

作者：朱敏生江苏省无锡市天一中学

本文探讨基因频率的计算中常见的方法，并提出对于红绿色盲基因频率的计算，“常规方法”是在任何情况下都是能够成立的，而“金榜方法”只有在“遗传平衡”时才能够成立的认识。

关键词：基因频率计算方法常规方法金榜方法

Trio,a Multifunctional Rho Guanine Nucleotide Exchange Factor,Is Necessary for the Migration of Cerebellar Granule Cells

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Trio,a Multifunctional Rho Guanine Nucleotide Exchange Facto...

中国遗传学会“发育、遗传和疾病”研讨会

作者： Yajing Peng Weiqi He minsheng zhu Model Animal Research Center,Nanjing University

Development of the mammalian central nervous system requires tightly regulated cellular events, including cell proliferation,and polarization,axon and dendrite out-growth *** this processes,small GTPases of the Rho family are crucial factors,which are coordinately regulated by complex signal pathways on GEFs and *** these signal pathways would offer insights into the mechanisms shared by neuronal developmental *** is a Multifunetional protein possessing two GEFs that probably act on Rae and *** data indicates that Trio genes in *** and Drosophila function in neural migration and axon guidance,trio completely deficient mice,which die during the late stages of embryonic development,display aberrant organization in neural *** findings imply that Trio may play an important role in neuronal development and the mechanism underlying may be involved in regulation of Rho GTPases'*** genetically determine Trio's in vivo functions in neural development,we floxed trio gene with loxP sites and generated trio-deficient mice (trioloxp/Ioxp;Nestin-Cre)with specific ablation of trio expression in the nervous system.95% homozygous mutants died within i d after birth,and the remaining died within 3 *** survived mice had remarkable ataxia and growth retardation during postnatal *** addition to disorganization of hippocampus and the olfactory bulb,trio null mice had significant structural abnormalities in cerebellum at P20,characterized by small size of cerebellum,disorganization of Purkinje cell layer(PCL)and no internal granule cell layer(IGL)*** P0 stage,the external granule cell layer(EGL)of trio-deficient mice was normally thick as the wide type mice,indicating that the migration processes of precursor cells from the rombic lip to the EGL was not affected. In order to determine granule cell migration status during cerebellum development in trio null mice,a bromodeoxyuridine labeling assay was perf

关键词： Trio,a Multifunctional Rho Guanine Nucleotide Exchange Factor,Is Necessary for the Migration of Cerebellar Granule Cells Rho

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味精废水的絮凝—吸附法预处理试验研究

水处理技术 1998年第5期24卷 299-302页

作者：黄民生朱莉上海大学环境系

在大量试验工作的基础上，本研究采用聚丙烯酸钠作为主要絮凝剂、木质素作为助凝剂、天然沸石作为吸附剂预处理味精浓废水，取得了十分好的效果。预处理过程对ＣＯＤ、ＳＳ、ＳＯ２－４的去除率分别达到６９％、９１％和４３％，预处理... 详细信息

在大量试验工作的基础上，本研究采用聚丙烯酸钠作为主要絮凝剂、木质素作为助凝剂、天然沸石作为吸附剂预处理味精浓废水，取得了十分好的效果。预处理过程对ＣＯＤ、ＳＳ、ＳＯ２－４的去除率分别达到６９％、９１％和４３％，预处理药剂费用约为６．２４元／吨废水，分离的ＳＳ蛋白经济获益约２７元／吨废水。

关键词：味精废水预处理絮凝吸附废水处理

iNOS N端融合蛋白在大肠杆菌中的表达及抗体制备

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中华微生物学和免疫学杂志 1999年第4期19卷 279-281页

作者：潘英朱敏生沈月许祥裕朱东亚朱有华陈强南京军区军事医学研究所中国药科大学

目的表达ｉＮＯＳＮ端片段并制备ｉＮＯＳ特异性抗体。方法将ｉＮＯＳＮ端片段装入原核表达载体ｐＥＴ－２８ａ（＋），在大肠杆菌ＢＬ２１中表达，Ｈｉｓ．ＢｉｎｄＴＭ柱亲和层析分离纯化及凝胶蛋白回收的两步纯化法纯化目的蛋白，使... 详细信息

目的表达ｉＮＯＳＮ端片段并制备ｉＮＯＳ特异性抗体。方法将ｉＮＯＳＮ端片段装入原核表达载体ｐＥＴ－２８ａ（＋），在大肠杆菌ＢＬ２１中表达，Ｈｉｓ．ＢｉｎｄＴＭ柱亲和层析分离纯化及凝胶蛋白回收的两步纯化法纯化目的蛋白，使用纯化蛋白制备ｉＮＯＳ多克隆抗体。结果得到具有较高表达量的融合蛋白，经两步纯化获得ｉＮＯＳＮ端融合蛋白纯品，将此蛋白免疫家兔，得到ｉＮＯＳ多克隆抗体，Ｗｅｓｔｅｒｎｂｌｏｔ证实该抗体具有较好的反应性及特异性。结论原核表达ｉＮＯＳＮ端片段制备ｉＮＯＳ抗体具有很好的特异性。

关键词： iNOS 重组蛋白特异性抗体抗体制备