检索结果-南通市图书馆

骨肉瘤患者血清中miR-34a、miR-449a表达及与新辅助化疗耐药的关系

在线全文

学校读者我要写书评

暂无评论

实用癌症杂志 2022年第3期37卷 388-391页

作者：楚利涛李兴华朱梁豫辛晓林魏瑄骆晓飞郑州市骨科医院 450005

目的分析骨肉瘤患者血清中miR-34a、miR-449a表达水平及与新辅助化疗耐药的关系。方法随机选择骨肉瘤83例,将其纳入研究组。另选取同期体检健康受试者83例,将其纳入对照组。检测血清miR-34a、miR-449a表达水平。问卷调查骨肉瘤患者临床... 详细信息

目的分析骨肉瘤患者血清中miR-34a、miR-449a表达水平及与新辅助化疗耐药的关系。方法随机选择骨肉瘤83例,将其纳入研究组。另选取同期体检健康受试者83例,将其纳入对照组。检测血清miR-34a、miR-449a表达水平。问卷调查骨肉瘤患者临床病理特征,给予新辅助化疗,统计耐药情况。结果研究组患者血清miR-34a水平(6.47±1.28)明显高于对照组(P0.05)。不同肿瘤大小、临床分期患者的血清miR-34a、miR-449a水平差异比较有统计学意义(P<0.05)。化疗有效组患者血清miR-34a表达水平(7.09±1.09)明显低于无效组(P<0.05),miR-449a表达水平(33.74±2.06)明显高于化疗无效组(P<0.05)。结论骨肉瘤患者血清miR-34a高表达,miR-449a低表达,且表达水平影响新辅助化疗效果,高表达会增加耐药性,增加化疗无效风险。

关键词：骨肉瘤 miR-34a miR-449a 新辅助化疗关系表达

基于16S rRNA技术与代谢组学探究枳椇果梗多糖对酒精暴露小鼠肠损伤的改善作用

在线全文

学校读者我要写书评

暂无评论

食品科学 2025年第3期46卷 110-118页

作者：张玉超朱思洁刘良禹王建云张佳欣苏泽雄陈江燕刘旭东茅台学院酿酒工程系贵州仁怀564507 茅台学院食品科学与工程系贵州仁怀564507 中国海洋大学食品科学与工程学院山东青岛266071

为探究枳椇果梗多糖(Hovenia dulcis fruit pedicel polysaccharides,HDPs)对酒精暴露小鼠肠道损伤的改善作用,采用16S rRNA高通量测序技术和代谢组学分析方法,研究HDPs对酒精暴露小鼠肠道微生物群和代谢谱的影响。同时,通过酶联免疫吸... 详细信息

为探究枳椇果梗多糖(Hovenia dulcis fruit pedicel polysaccharides,HDPs)对酒精暴露小鼠肠道损伤的改善作用,采用16S rRNA高通量测序技术和代谢组学分析方法,研究HDPs对酒精暴露小鼠肠道微生物群和代谢谱的影响。同时,通过酶联免疫吸附试验、免疫组化、实时定量聚合酶链式反应和Western blot等方法,检测肠道炎症因子、紧密连接蛋白、胆汁酸代谢相关基因和蛋白的表达变化。研究结果表明,HDPs能显著降低酒精诱导的肠道促炎因子白细胞介素-4、干扰素-γ和肿瘤坏死因子α的水平,减少内毒素脂多糖和脂多糖结合蛋白的水平,提高α-淀粉酶活力,上调紧密连接蛋白Claudin-1从而改善肠道屏障功能。16S rRNA测序结果显示,与灌胃114μL/20 g mb酒精的小鼠相比,HDPs能增加小鼠肠道菌群中乳杆菌属(Lactobacillus)的相对丰度,改善酒精暴露所致肠道微生物群多样性和结构紊乱。代谢组学分析发现,HDPs能够调节胆汁酸代谢,降低酒精暴露小鼠肠道中胆汁酸(特别是牛磺胆酸、鹅脱氧胆酸)的水平。此外,HDPs还能抑制酒精暴露小鼠肠道中顶端钠依赖性胆酸转运体mRNA和蛋白水平的表达,减少胆汁酸的重吸收,从而减轻酒精对肠道的负面影响。综上,HDPs可通过调节肠道菌群和胆汁酸代谢,改善肠道屏障功能,对酒精性肠损伤具有潜在的改善作用,本研究结果可为HDPs在功能性食品中应用提供新的视角和理论依据。

关键词：枳椇果梗多糖酒精性肠损伤肠道菌群胆汁酸代谢改善作用

Study on conductance of supersaturated chloride microdroplets

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2009年第7期52卷 879-886页

作者： HE KeJuan CHENG Hua zhu YanYing WANG liangyu ZHANG YunHong Institute for Chemical Physics School of ScienceBeijing Institute of TechnologyBeijing 100081China

By using the measuring system previously designed by the authors, the conductance of KCl, NaCl and NH4Cl microdroplets is obtained in the whole measuring RH range, especially in the supersaturation region, which cannot be acquired from the bulk solutions and fills the gap of lack of experimental data of conductance under the supersaturated state. The ERH and DRH of these three kinds of microdroplets observed from a microscope are 80.5% and 95.4% (KCl), 75.7% and 93.3% (NaCl), and 69.9% and 96.6% (NH4Cl), respectively. In addition, it can be found from the dependence of conductance on RH that conductance is very sensitive to the existence of water molecules inside the microdroplet and the threshold of the deliquescence process can be predicted by the variation of conductance.

关键词： conductance supersaturation microdroplet chloride sodium chloride potassium chloride ammonium chloride

miRNA-153-5p通过靶向TFRC调控巨噬细胞极化在病毒性心肌炎中的作用

在线全文

学校读者我要写书评

暂无评论

皖南医学院学报 2024年第3期43卷 205-209页

作者：张苑李雪琴朱良宇周汉宇钟民吕坤皖南医学院第一附属医院弋矶山医院中心实验室安徽芜湖241001

目的:探讨miRNA-153-5p调控巨噬细胞极化在病毒性心肌炎(VM)中的作用。方法:建立VM小鼠模型,提取心脏,RT-qPCR检测心肌组织miRNA-153-5p的表达,HE染色观察心脏炎症程度,ELISA检测小鼠血清肌钙蛋白cTnI的水平,流式细胞术检测心脏浸润巨... 详细信息

目的:探讨miRNA-153-5p调控巨噬细胞极化在病毒性心肌炎(VM)中的作用。方法:建立VM小鼠模型,提取心脏,RT-qPCR检测心肌组织miRNA-153-5p的表达,HE染色观察心脏炎症程度,ELISA检测小鼠血清肌钙蛋白cTnI的水平,流式细胞术检测心脏浸润巨噬细胞表型。体外分离BMDMs诱导为M1/M2型巨噬细胞,过表达和抑制miR-153-5p,RT-qPCR检测M1/M2标志物iNOS、IL-12、TNF-α、Arg1、YM-1、FIZZ1的表达。生物信息学软件预测结合双荧光素酶实验验证miR-153-5p与转铁蛋白受体(TFRC)的靶向关系。结果:miR-153-5p在CVB3感染7 d的VM小鼠心脏组织中表达明显增加(P<0.05)。体内实验中抑制miR-153-5p表达能减轻VM小鼠心脏的病变程度,改善心脏功能(P<0.01)。抑制miR-153-5p表达能够促进小鼠心脏浸润的巨噬细胞向M2极化(P<0.01)。体外实验中,较之M2,miR-153-5p在M1巨噬细胞中表达升高(P<0.01)。过表达miR-153-5p促进巨噬细胞向M1极化,抑制miR-153-5p表达则促进巨噬细胞向M2极化(P<0.01)。TFRC是miR-153-5p的靶基因,抑制miR-153-5p表达可上调TFRC的mRNA和蛋白水平(P<0.01)。结论:miRNA-153-5p通过靶向TFRC调控VM小鼠心脏浸润巨噬细胞的极化。

关键词： miR-153-5p 巨噬细胞转铁蛋白受体病毒性心肌炎

Effect of Shot Peening on Surface Damage Evolution Behavior of Cu-19Ni Alloy

在线全文

学校读者我要写书评

暂无评论

Journal of Wuhan University of Technology(Materials Science) 2023年第1期38卷 212-221页

作者：朱旭军李磊 SHI Zhiming WEI liangyu TIAN Fuzheng College of Sciences Inner Mongolia University of TechnologyHohhot 010051China School of Materials Science and Engineering Inner Mongolia University of TechnologyHohhot 010051China

Shot peening is a surface modification technology with the metal surface nano machine(SNC),which can modify the surface microstructure and extend the fatigue life of Cu-19Ni *** hardness,damage evolution and mechanical properties were investigated and characterized by scanning electron microscope(SEM),laser confocal microscope(LSM)and material surface performance tester(CFT).The results showed that the surface roughness and friction coefficient of Cu-19Ni alloy decreased with the increase of shot peening duration and diameter,while the microhardness and strength ***,with the increase in shot peening duration and diameter,SEM observation showed that the fracture dimples became smaller,meanwhile,with the increase of small cleavage planes,shear tearing ridges and the thickness of the surface nano layer,the fracture mode gradually evolved from plastic to brittle *** uniaxial tensile test of shot peened Cu-19Ni alloy was carried out by MTS testing machine combined with digital image correlation technology(DIC).The evolution of Cu-19Ni surface damage was analyzed,and the evolution equations describing the damage of large deformation zone and small deformation zone were *** effect of shot peening on the damage evolution behavior of Cu-19Ni alloy was revealed.

关键词： Cu-19Ni alloy shot peening damage evolution mechanical properties digital image correlation

在线全文

学校读者我要写书评

暂无评论

核电厂一回路典型核素探测效率标定方法研究

湖北电力 2020年第2期44卷 1-5页

作者：丁锡嘉周涛张家磊张博雅陈娟朱亮宇华北电力大学核科学与工程学院北京102206 华北电力大学核热工安全与标准化研究所北京102206 非能动核能安全技术北京市重点实验室北京102206 东南大学能源与环境学院江苏南京211189

核电厂一回路典型放射性核素探测效率标定实验对核电厂最终一回路泄漏率的确定和海洋环境中放射性核素监测具有重要作用。将18F作为监测核电厂一回路压力边界的泄漏率的理想核素,采用实验与MCNP模拟两种方法完成了18F的探测效率标定,表... 详细信息

核电厂一回路典型放射性核素探测效率标定实验对核电厂最终一回路泄漏率的确定和海洋环境中放射性核素监测具有重要作用。将18F作为监测核电厂一回路压力边界的泄漏率的理想核素,采用实验与MCNP模拟两种方法完成了18F的探测效率标定,表明在一定区间内,随着18F源与探测器探头间距离平方倒数的增加,探测器对18F的探测效率逐渐增加。通过实验与模拟两种方法,证明18F的探测效率符合平方反比定律,实验结论成立。根据实验数据,得出18F的探测效率经验公式。

关键词：核电厂一回路探测效率标定平方反比定律

在线全文

学校读者我要写书评

暂无评论

基于Pub Med越桔研究现状分析

国土与自然资源研究 2022年第5期 78-80页

作者：朱良玉王化李梦莎魏殿文何丹娆周丽萍黑龙江省科学院自然与生态研究所哈尔滨150040 东北林业大学哈尔滨150040

基于Pub Med数据库,开展SCI文献统计分析,从数量、研究类型、内容及期刊角度分析2016—2021年国际越桔研究趋势。结果表明近6年间,国际越桔研究论文的数量呈增长趋势,实验研究类文献是主要的研究类型,研究内容主要是越桔成分分析、越桔... 详细信息

基于Pub Med数据库,开展SCI文献统计分析,从数量、研究类型、内容及期刊角度分析2016—2021年国际越桔研究趋势。结果表明近6年间,国际越桔研究论文的数量呈增长趋势,实验研究类文献是主要的研究类型,研究内容主要是越桔成分分析、越桔资源研究和越桔微生物功能研究。发表最多有关越桔研究内容的期刊是Food Chemistry,研究收录方向是化学、食品科学与技术和营养学。

关键词：越桔文献分析 Pub Med

Experimental Test of Contextuality Based on State Discrimination with a Single Qubit

在线全文

学校读者我要写书评

暂无评论

Chinese Physics Letters 2022年第8期39卷 1-5页

作者： Qiuxin Zhang Chenhao zhu Yuxin Wang liangyu Ding Tingting Shi Xiang Zhang Shuaining Zhang Wei Zhang Department of Physics Renmin University of ChinaBeijing 100872China Beijing Academy of Quantum Information Sciences Beijing 100193China Beijing Key Laboratory of Opto-electronic Functional Materials and Micro-nano Devices Renmin University of ChinaBeijing 100872China

Exploring quantum phenomena beyond predictions of any classical model has fundamental importance to understand the boundary of classical and quantum descriptions of *** a typical property that a quantum system behaves distinctively from a classical counterpart,contextuality has been studied extensively and verified experimentally in systems composed of at least three levels(qutrit).Here we extend the scope of experimental test of contextuality to a minimal quantum system of only two states(qubit)by implementing the minimum error state discrimination on a single171Yb+*** observe a substantial violation of a no-go inequality derived by assuming non-contextuality,and firmly conclude that the measured results of state discrimination cannot be reconciled with any non-contextual *** also quantify the contextual advantage of state discrimination and the tolerance against quantum noises.

关键词： quantum system inequality

Innovations and stepwise evolution of CBFs/DREB1s and their regulatory networks in angiosperms

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2022年第11期64卷 2111-2125页

作者： Yuqi Nie liangyu Guo Fuqiang Cui Yirong Shen Xiaoxue Ye Deyin Deng Shuo Wang Jianhua zhu Wenwu Wu State Key Laboratory of Subtropical Silviculture School of Forestry and BiotechnologyZhejiang A&F UniversityHangzhou 311300China Institute of Tropical Biosciences and Biotechnology Chinese Academy of Tropical Agricultural SciencesHaikou 571101China School of Life Sciences Anhui Agricultural UniversityHefei 230036China Department of Plant Science and Landscape Architecture University of MarylandCollege ParkMaryland 20742USA

The C-repeat binding factors/dehydrationresponsive element binding protein 1 s(CBFs/DREB1 s)have been identified as major regulators of cold acclimation in many angiosperm ***,their origin and evolutionary process associated to cold responsiveness are still *** integrating multi-omics data of genomes,transcriptomes,and CBFs/DREB1 s genome-wide binding profiles,we unveil the origin and evolution of CBFs/DREB1 s and their regulatory *** collinearity and phylogeny analyses show that CBF/DREB1 is an innovation evolved from tandem duplication-derived DREBⅢgene.A subsequent event of e-whole genome duplication led to two CBF/DREB1 archetypes(CladesⅠandⅡ)in ancient *** contrast to cold-insensitivity of Clade I and their parent DREBⅢgenes,CladeⅡevolved a further innovation in cold-sensitive response and was stepwise expanded in eudicots and monocots by independent *** geological time,the duplication events were mainly enriched around the Cretaceous-Paleogene(K-Pg)boundary and/or in the Late Cenozoic Ice Age,when the global average temperature significantly ***,the duplicated CBF/DREB1 genes contributed to the rewiring of CBFs/DREB1 s-regulatory network for cold ***,our results highlight an origin and convergent evolution of CBFs/DREB1 s and their regulatory network probably for angiosperms adaptation to global cooling.

关键词： CBF/DREB1 cold acclimation global cooling ice age K-Pg boundary paleotemperature tandem duplication(TD) whole genome duplication(WGD)