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ST13,a proliferation regulator,inhibits growth and migration of colorectal cancer cell lines

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第11期13卷 884-893页

作者： Rui bai Zhong SHI Jia-wei zhang Dan li yong-liang zhu Shu zheng Cancer Institute (Key Laboratory of Cancer Prevention and Intervention China National Ministry of Education Key Laboratory of Molecular Biology in Medical Sciences Zhejiang Province China) the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China Department of Gastroenterology the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China

Background and objective:ST13, is the gene encoding the HSP70 interacting protein (HIP). Previous research has shown that ST13 mRNA and protein levels are down-regulated in colorectal cancer (CRC) tissues compared with adjacent normal tissues. This study aims at the role of ST13 in the proliferation and migration of CRC cells. Methods:The transcript level of ST13 in different CRC cell lines was evaluated by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). ST13-overexpressed and ST13-knockdown CRC cells were constructed respectively by lentiviral transduction, followed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay, plate colony formation, cell-cycle analysis, and migration assays to evaluate the influence of ST13 on proliferation and migration in vitro. Moreover, a mouse xenograft study was performed to test in vivo tumorigenicity of ST13-knockdown CRC cells. Results: Lentivirus-mediated overexpression of ST13 in CRC cells in-hibited cell proliferation, colony formation, and cell migration in vitro. In contrast, down-regulation of ST13 by lentiviral-based short hairpin RNA (shRNA) interference in CRC cells significantly increased cell proliferation and cloning efficiency in vitro. In addition, down-regulation of ST13 expression significantly increased the tumorigenicity of CRC cells in vivo. Conclusions:ST13 gene is a proliferation regulator that inhibits tumor growth in CRC and may affect cell migration.

关键词： Colorectal cancer ST13 Proliferation Colony formation Cell cycle Migration

Electrochemical adsorption and passivation on gold surface in alkaline thiourea solutions

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Rare Metals 2020年第8期39卷 951-958页

作者： Wen-Juan li He Zhou An-Ping bai yong-Sheng Song liu-Lu Cai Shui-lin zheng Qi-Dong zhang Song cao School of Chemical and Environmental Engineering China University of Mining and Technology(Beijing)Beijing 100083China GRIMAT Engineering Institute Co. Ltd.(GRIMAT)Beijing 101407China National Engineering Laboratory of Biohydrometallurgy GRINM Resources and Environment Tech.Co.LtdBeijing 101407China Beijing Vocational College of Labour and Social Security Beijing 102200China

An electrochemical method was used to examine Au surface absorption and passivation under the most commonly employed pH conditions in an alkaline Au leaching *** polarization at different pH values was obtained through the current step *** pH 10,no absorption layer was *** the pH was increased to 11,an absorption layer was formed through the Au electrode *** pH 12,the entire system could not be stabilized,even after long durations because of the thiourea oxidation and *** samples were analyzed by scanning electron microscopy(SEM),energy-dispersive X-ray spectroscopy(EDS),and atomic force microscopy(AFM).AFM observations of the Au plating surface and SEM-EDS analyses for the Au-coated graphite surface indicated that a passivation membrane was formed on the Au surface after its use in the alkaline thiourea Au leaching ***-step leaching was used to confirm the conclusions drawn from the experimental *** twostage experimental results further confirmed the existence of a passivation membrane.

关键词： Alkaline thiourea Gold Electrochemical adsorption Passivation

A de novo frameshift mutation of the SRY gene leading to a patient with 46,XY complete gonadal dysgenesis

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Asian Journal of Andrology 2019年第5期21卷 522-524页

作者： Xiao-Bo Wang Yu-long liang Zi-Jue zhu yong zhu Peng li Jia-Ping cao Qun-Ying zhang Qiang liu zheng li Department of Andrology Center for Men's HealthInstitute of UrologyUrologic Medical CenterShanghai General HospitalShanghai 200080China Shanghai Key Laboratory of Reproductive Medicine Department of HistoembryologyGenetics and Developmental BiologyShanghai Jiao Tong University School of MedicineShanghai 200025China Department of Gynecology and Obstetrics Changxing Maternity and Child Health Care HospitalHuzhou 313199China

Dear Editor,Sex in mammals is genetically determined and defined at the cellular level by the sex chromosome constitution(XY males and XX females)and at the phenotypic level by the development of genderspecific anatomy,physiology,and behavior.146,XY complete gonadal dysgenesis(46,XY CGD),first reported by Swyer in 1955,^2 is a rare congenital condition with completely or partially disordered gonadal development,leading to discordance between the genetic,gonadal,and phenotypic *** patients have a 46,XY karyotype,a female phenotype,normal female external genitalia,and CGD("streak gonads")without sperm production and follicular or steroid function.

关键词： 46,XY anatomy gonadal

Design of an energetic particle radiation diagnostic spectroscopy system based on national core chips and Qt on linux in EAST

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Nuclear Science and Techniques 2021年第7期32卷 11-21页

作者： Ying-Ying zheng Zi-Han zhang Qiang li Hong-Rui cao yong-Qiang zhang Jin-long Zhao Qi-Ping Yuan Bing-Jia Xiao ling-ling Yan Jian-Qiu zhu Institute of Plasma Physics Hefei Institutes of Physical ScienceChinese Academy of SciencesHefei 230031China University of Science and Technology of China Hefei 230026China Hefei University of Technology Hefei 230009China

Energetic particle radiation diagnoses mainly detect the particles(such as neutrons,gamma rays,hard X-rays,and escaping electrons)that are radiated in the discharge process of the experimental advanced superconducting tokamak device to characterize the operating state of the plasma in real *** upgrading of these diagnoses requires new instruments based on national(here,“national”means developed and produced by a Chinese company)core chips and open-source software with advanced digitization,a high sampling rate,and a high time *** new spectroscopy system designed in this study adopts the national field-programmable gate array(FPGA)and an analog-to-digital converter as the core chip,and it is developed using Qt on *** communication between the FPGA and embedded controller occurs via a high-speed peripheral component interconnect eXtension for instrument express protocol with a direct memory access *** this basis,the time resolution of the system is improved from 2 to 1 ms,the maximum channel address is increased to 4096,and the sampling rate is increased from 10 to 80 *** experiments of the spectroscopy system with 152Eu and 137Cs sources demonstrate that the best energy resolution is 0.27%and the measurement error is less than±0.5 keV.

关键词： Spectroscopy system National chip Qt Energetic particle radiation Experimental advanced superconducting tokamak

Inflammatory biomarkers and cerebral small vessel disease:a community-based cohort study

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Stroke & Vascular Neurology 2022年第4期7卷 302-309,I0009-I0014页

作者： Ding-Ding zhang Yuan cao Jing-Yu Mu Yi-ming liu Feng Gao Fei Han Fei-Fei Zhai li-Xin Zhou Jun Ni ming Yao ming-li li zheng-Yu Jin Shu-Yang zhang li-Ying Cui yong Shen Yi-Cheng zhu Central Research Laboratory State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina Department of Neurology State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina Division of life Sciences and Medicine Institute on Aging and Brain DisordersThe First Affiliated Hospital of USTCUniversity of Science and Technology of ChinaHefeiChina Department of Radiology State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina Department of Cardiology State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina

Background and purpose Although inflammation has been proposed to be a candidate risk factor for cerebral small vessel disease(CSVD),previous findings remain largely inconclusive and vary according to disease status and study *** present study aimed to investigate possible associations between inflammatory biomarkers and MRI markers of *** A group of 15 serum inflammatory biomarkers representing a variety of those putatively involved in the inflammatory cascade was grouped and assessed in a cross-sectional study involving 960 stroke-free *** biomarker panel was grouped as follows:systemic inflammation(high-sensitivity C reactive protein(hsCRP),interleukin 6 and tumour necrosis factorα),endothelial-related inflammation(E-selectin,P-selectin,intercellular adhesion molecule 1,vascular cell adhesion molecule 1(VCAM-1),CD40 ligand,lipoprotein-associated phospholipase A2,chitinase-3-like 1 protein and total homocysteine(tHCY))and media-related inflammation(matrix metalloproteinases 2,3 and 9,and osteopontin).The association(s)between different inflammatory groups and white matter hyperintensity(WMH),lacunes,cerebral microbleeds(CMBs),enlarged perivascular space(PVS)and the number of deep medullary veins(DMVs)were *** High levels of serum endothelial-related inflammatory biomarkers were associated with both increased WMH volume(R^(2)=0.435,p=0.015)and the presence of lacunes(R^(2)=0.254,p=0.027).Backward stepwise elimination of individual inflammatory biomarkers for endothelial-related biomarkers revealed that VCAM-1 was significant for WMH(β=0.063,p=0.005)and tHCY was significant for lacunes(β=0.069,plimination of individual systemic-related inflammatory biomarkers revealed that hsCRP(β=−0.162,p=0.007)was *** WM

关键词： inflammation cerebral stepwise

Synergistic Effect of Triple Ion Beams on Radiation Damage in CLAM Steel

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Chinese Physics Letters 2014年第4期31卷 93-95页

作者： YUAN Da-Qing zheng yong-Nan ZUO Yi FAN Ping ZHOU Dong-Mei zhang Qiao-li MA Xiao-Qiang CUI bao-Qun CHEN li-Hua JIANG Wei-Sheng WU Yi-Can ttUANG Qun-Ying PENG Lei cao Xing-Zhong WANG bao-Yi WEI long zhu Sheng-Yun China Institute of Atomic Energy Beijing 102413 Institute of Nuclear Energy Safety Technology Chinese Academy of Sciences Hefei 230031 Institute of High Energy Physics Chinese Academy of Sciences Beijing 100039

The synergistic effect of triple ion beams is investigated by simultaneous and scquential irradiations of gold, hydrogen and helium ions on the low activation martensitie steel （CLAM） developed in China. The depth profile measurements of the positron annihilation Doppler broadening S parameter are carried out as a function of slow- positron beam energy to examine the produced radiation damage. The synergistic effect of displacement damage and hydrogen and helium on the formation of radiation damage is clearly observed. In the preset case ,this effect suppresses the radiation damage in the CLAM steel due to the helium and/or hydrogen filling of vacancy clusters.

关键词： Steel Synergistic low

A proof of concept for neutralizing antibody-guided vaccine design against SARS-CoV-2

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National Science Review 2021年第8期8卷 147-157页

作者： li zhang Lei cao Xing-Su Gao Bin-Yang zheng yong-Qiang Deng Jing-Xin li Rui Feng Qian Bian Xi-ling Guo Nan Wang Hong-Ying Qiu Lei Wang Zhen Cui Qing Ye Geng Chen Kui-Kui Lu Yin Chen Yu-Tao Chen Hong-Xing Pan Jiaping Yu Wenrong Yao bao-li zhu Jianping Chen yong liu Cheng-Feng Qin Xiangxi Wang Feng-Cai zhu National Health Commission of the People’s Republic of China Key Laboratory of Enteric Pathogenic Microbiology (Jiangsu Provincial Center for Disease Control and Prevention) Center for Global Health School of Public HealthNanjing Medical University CAS Key Laboratory of Infection and Immunity National Laboratory of MacromoleculesInstitute of BiophysicsChinese Academy of Sciences Guangzhou Regenerative Medicine and Health Guangdong Laboratory Jiangsu Rec-biotechnology Co.Ltd State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology

Mutations and transient conformational movements of the receptor binding domain(RBD) that make neutralizing epitopes momentarily unavailable present immune escape routes for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2). To mitigate viral escape, we developed a cocktail of neutralizing antibodies(NAbs) targeting epitopes located on different domains of spike(S) protein. Screening of a library of monoclonal antibodies generated from peripheral blood mononuclear cells of COVID-19 convalescent patients yielded potent NAbs, targeting the N-terminal domain(NTD) and RBD domain of S, effective at n M concentrations. Remarkably, a combination of RBD-targeting NAbs and NTD-binding NAbs, FC05, enhanced the neutralization potency in cell-based assays and an animal model. Results of competitive surface plasmon resonance assays and cryo-electron microscopy(cryo-EM) structures of antigen-binding fragments bound to S unveil determinants of immunogenicity. Combinations of immunogens, identified in the NTD and RBD of S, when immunized in rabbits and macaques, elicited potent protective immune responses against SARS-CoV-2. More importantly, two immunizations of this combination of NTD and RBD immunogens provided complete protection in macaques against a SARS-CoV-2 challenge, without observable antibody-dependent enhancement of infection. These results provide a proof of concept for neutralization-based immunogen design targeting SARS-CoV-2 NTD and RBD.

关键词： SARS-CoV-2 neutralizing antibodies vaccine development structure-based immunogen design

Site-testing at the Muztagh-ata Site *** Cloud Amount during the Last Five Years

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Research in Astronomy and Astrophysics 2023年第4期23卷 164-171页

作者： Jing Xu Guo-jie Feng Guang-xin Pu Le-tian Wang Zi-Huang cao li-Qing Ren Xuan zhang Shu-guo Ma Chun-hai bai Ali Esamdin Jian li Yuan Tian zheng Wang yong-heng Zhao Jian-rong Shi Xinjiang Astronomical Observatory Chinese Academy of SciencesUrumqi 830011China National Astronomical Observatories Chinese Academy of SciencesBeijing 100101China Urumqi Meteorological Satellite Ground Station Urumqi 830011China

The clarity of nights is the major factor that should be carefully considered for optical/infrared astronomical observatories in site-testing *** coverage is directly related to the amount of time available for scientific observations at *** this article,we report on the results of detailed night-time cloud statistics and continuous observing time derived from ground-based all-sky cameras(ASCs)at the Muztagh-ata site from 2017to *** obtained from acquisition data show that the proportion of the annual observing time at the Muztagh-ata site is 65%,and the best period with the least cloud coverage and longer continuous observing time is from September to *** made a comparison of the monthly mean observing nights obtained from our ASC and CLARA data set,and results show that the discrepancy between them may depend on the cloud top *** average,this site can provide 175 clear nights and 169 nights with at least 4 hr of continuous observing time per year.

关键词： site testing-atmospheric effects-methods statistical

Structure-Spin-Transport Anomaly in Quasi-One-Dimensional Ba9Fe3Te15 under High Pressure

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Chinese Physics Letters 2020年第8期37卷 101-107页

作者： Jun zhang Mei-ling Jin Xiang li Xian-Cheng Wang Jian-Fa Zhao Ying liu Lei Duan Wen-Min li li-Peng cao Bi-Juan Chen li-Juan Wang Fei Sun yong-Gang Wang liu-Xiang Yang Yu-ming Xiao zheng Deng Shao-Min Feng Chang-Qing Jin Jin-long zhu Center for High Pressure Science&Technology Advanced Research Beijing 100094China Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190China School of Physics University of Chinese Academy of SciencesBeijing 100190China Key Laboratory of Advanced Optoelectronic Quantum Architecture and Measurement(Ministry of Education) School of PhysicsBeijing Institute of TechnologyBeijing 100081China Xi'an Modern Chemistry Research Institute Xi'an 710065China High Pressure Collaborative Access Team(HPCAT) Advanced Photon SourceArgonne National LaboratoryArgonneIllinois 60439USA Songshan Lake Materials Laboratory Dongguan 523808China Department of Physics Southern University of Science and TechnologyShenzhen 518055China

Recently,a series of novel compounds Ba3MX5(M=Fe,Ti,V;X=Se,Te)with hexagonal crystal structures composed of quasi-1-dimensional(1D)magnetic chains has been synthesized by our research team using high-pressure and high-temperature *** initial hexagonal phases persist to the maximum achievable pressure,while spin configurations and magnetic interactions may change dramatically as a result of considerable reductions in interchain separations upon *** compounds therefore offer unique possibilities for studying the evolution of intrinsic electronic structures in quasi-1D magnetic *** we present a systematic investigation of Ba9Fe3Te15,in which the interchain separations between trimerized 1D chains(~10.2Å)can be effectively modulated by external high *** crystal structure especially along the 1D chains exhibits an abnormal expansion at^GPa,which accompanies trimerization entangled anomalous mixed-high-low spin *** insulator-metal transition has been observed under high pressure as a result of charge-transfer gap ***-induced superconductivity emerges at 26 GPa,where the charge-transfer gap fully closes,3D electronic configuration forms and local spin fully collapses.

关键词： transition. hexagonal chains