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Journal of Animal Science and Biotechnology 2024年第3期15卷 971-986页

作者： Haorong He Yuanhang Wei Yuqi Chen Xiyu Zhao Xiaoxu Shen Qing zhu Huadong Yin Key Laboratory of Livestock and Poultry Multi‑Omics Ministry of Agriculture and Rural AffairsCollege of Animal Science and TechnologySichuan Agricultural UniversityChengduSichuan 611130China Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengduSichuan 611130China

Background The reproductive performance of chickens mainly depends on the development of ***-mal follicle development can lead to decreased reproductive performance and even ovarian disease among *** is the only non-human animal with a high incidence of spontaneous ovarian *** recent years,the involvement of circRNAs in follicle development and atresia regulation has been *** In the present study,we used healthy and atretic chicken follicles for circRNA *** results showed differential expression of *** was then confirmed that circRALGPS2 can translate into a protein,named cir-cRALGPS2-212aa,which has IRES ***,we found that circRALGPS2-212aa promotes apoptosis and autophagy in chicken granulosa cells by forming a complex with PARP1 and *** Our results revealed that circRALGPS2 can regulate chicken granulosa cell apoptosis and autophagy through the circRALGPS2-212aa/PARP1/HMGB1 axis.

关键词： Apoptosis Autophagy Chicken CircRALGPS2 Follicle atresia PARP1 RNC-seq

Combination strategies for first-line treatment of patients with unresectable hepatocellular carcinoma:prospect of natural products

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Chinese Journal of Natural Medicines 2024年第1期22卷 1-3页

作者： zhang Lele LU Jinjian school of Basic Medical Sciences Chengdu UniversityChengdu 610106China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao 999078China Department of Pharmaceutical Sciences Faculty of Health SciencesUniversity of MacaoMacao 999078China

Liver cancer stands as a significant global health concern,contributing substantially to cancer incidence and mortality,particularly in Asian countries[1].Hepatocellular carcinoma(HCC)accounts for approximately 90%of all liver cancer cases and is characterized by a high-risk profile and a generally poor prognosis[2].To address advanced HCC,systemic therapy has been recommended,leading to the approval of a range of treatment regimens in clinical ***,first-line therapy involved the use of multitargeted tyrosine kinase inhibitors(TKIs)such as sorafenib or lenvatinib,while cabozantinib,ramucirumab.

关键词： Unresectable hepatocellular carcinoma Combination strategies Immunotherapy PD-1/PD-L1 Natural products

AGRICULTURAL STANDARDIZATION IN china

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AGRICULTURAL STANDARDIZATION IN CHINA

Application of High-Resolution Sequence Stratigraphy to Precise Lacustrine Reservoir Correlations with an example from Linpan Oil Fleld,china

The 3rd International Symposium on Intelligent Information Technology in Agriculture(ISIITA)

作者： Yah Xue~1 Yeping zhu~1 Ling Xue~2 ~1Institute of Agricultural Information Chinese Academy of Agricultural Sciences Beijing 100081 china ~2The school of Government Peking university Beijing 100871 china

＜正＞china sees a dramatic transition from traditional agriculture to modern agriculture in which agricultural standardization is an indispensable prerequisite for developing modern *** paper discusses the status quo and existing problems in the development of agricultural standardization recent years in this country, and puts forward suggestions and countermeasures for agricultural standardization system building,promotion, implementation and supervision.

关键词： Agricultural Standar-dization System Promotion Implementation Supervision

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沉积学报 1998年第2期 34-41页

作者： Jiang Zaixing1\ Cao Yingchang1\ Chang Lin1\ Ye Lianjun2 Li Renwei2Zhou Xiangquan 3\ zhang Yong 3\ Wang Ximing 3\ Du Yumin 31(Dept.of Petroleum Resources Sciences,Dongying,Shandong\ 257062) 2(Geological Institute,Chinese Academy of Science

１ＩＮＴＲＯＤＵＣＴＩＯＮＨｉｇｈ－ｒｅｓｏｌｕｔｉｏｎｓｅｑｕｅｎｃｅｓｔｒａｔｉｇｒａｐｈｉｃｔｅｃｈ－ｎｉｑｕｅｈａｓｓｕｃｃｅｓｓｆｕｌｙｂｅｅｎｕｓｅｄｔｏｐｒｅｄｉｃｔｍａｒｉｎｅａｎｄｄｅｌｔａｉｃｒｅ... 详细信息

１ＩＮＴＲＯＤＵＣＴＩＯＮＨｉｇｈ－ｒｅｓｏｌｕｔｉｏｎｓｅｑｕｅｎｃｅｓｔｒａｔｉｇｒａｐｈｉｃｔｅｃｈ－ｎｉｑｕｅｈａｓｓｕｃｃｅｓｓｆｕｌｙｂｅｅｎｕｓｅｄｔｏｐｒｅｄｉｃｔｍａｒｉｎｅａｎｄｄｅｌｔａｉｃｒｅｓｅｒｖｏｉｒｓｓｉｎｃｅ１９９０...

关键词： Lake level change\ Sequence stratigraphy\ Reservoir Correlation

Ceramide induces release of mitochondrial proapop-totic proteins in caspase-dependent and -independent manner in HT-29 cells

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Science china(Life Sciences) 2008年第1期51卷 66-71页

作者： zhang XiaoFeng1, LI BaiXiang1, zhang Yang1 & LIU JiaRen2 1 Public Health College, Harbin Medical university, Harbin 150081, china 2 Department of Food Science, Cornell university, New York 14853-7201, USA Public Health College Harbin Medical University Harbin 150081 China Department of Food Science Cornell University New York 14853-7201 USA

In this study, the release of mitochondrial proapoptotic intermembrane space proteins induced by ex-ogenous C2-ceramide in human colon carcinoma (HT-29) cell line was investigated. HT-29 cells were treated with 12.5, 25 and 50 μmol/L C2-ceramide in vitro. Flow cytometer was used to detect the mito-chondrial membrane potential (ΔΨm). Subcellular fractions were extracted by Mitochondrial/Cytosol Fractionation Kit after C2-ceramide treatment for 24 h. SDS-PAGE was used to determine the level of cytochrome c (Cyt c), high temperature requirement A2 (HtrA2) and second mitochondrial-derived ac-tivator of caspases (Smac) released from mitochondria, the expression of X-linked inhibitor of apop-tosis protein (XIAP) and caspase-3 for 24 h. The results showed that ΔΨm began to decrease from 6 h after 25 and 50 μmol/L C2-ceramide treatment (P2-ceramide on the expression of Cyt c, HtrA2 and Smac in the total levels. 12.5, 25 and 50 μmol/L C2-ceramide could induce Cyt c, HtrA2 and Smac to release from mitochondria to cytosol and down-regulate the expression of XIAP (P2-ceramide treatment. After the treatment with caspase inhibitor, C2-ceramide still induced the release of Cyt c and HtrA2, but Smac did not. Therefore, C2-ceramide could induce apoptosis of HT-29 cells through the mitochondria pathway. The release of Cyt c, HtrA2 and Smac from mitochondria did not occur via the same mechanism, the release of Cyt c and HtrA2 was caspase-independent and the re-lease of Smac was caspase-dependent.

关键词： C_2-ceramide mitochondria proapoptotic intermembrane space proteins mitochondrial membrane potential human colon carcinoma cells caspase

Practical Synthesis of Valbenazine via 1,3-Dipolar Cycloaddition

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Chinese Journal of Chemistry 2024年第8期42卷 841-845页

作者： Yalan Peng Zuming Lin Lili zhu Shiqing Han Sha-Hua Huang Ran Hong College of Biotechnology and Pharmaceutical Engineering Nanjing Tech UniversityNanjingJiangsu211816 China school of Environmental and Chemical Engineering Shanghai Institute of TechnologyShanghai201418 China State Key Laboratory of Chemical Biology Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai200032 China Innovation Research Institute of Traditional Chinese Medicine(IRI) Shanghai University of Traditional Chinese Medicine1200 Cailun RoadShanghai201203 China

Comprehensive Summary,Valbenazine(Ingrezza),a potent and highly selective inhibitor of vesicular monoamine transporter type 2(VMAT2)through the active metabolite hydrotetrabenazine(HTBZ),has been approved for the treatment of tardive dyskinesia and,very recently,for chorea,which is associated with Huntington's *** numerous synthetic efforts dedicated to the synthesis of HTBZ,the industrial preparation of valbenazine uses dihydroisoquinoline as a starting material and the chiral resolution of racemic HTBZ derived from ketone ***,we present a practical synthesis of HTBZ and valbenazine featuring a highly stereoselective 1,3-dipolar cycloaddition and enzymatic kinetic *** cascade process includes cycloaddition,N—O bond cleavage,and lactamization,which proved to be operationally *** allure of the enzymatic resolution developed in this work offers a rapid access toward affording tetrahydroisoquinoline(THIQ)-fused piperidine in the production of medically significant compounds,such as yohimbine and reserpine.

关键词： Cycloaddition Chemoenzymatic Kinetic resolution Prodrugs VMAT2 inhibitor

Characteristics and quality of clinical practice guidelines for diabetic kidney disease:a systematic review

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Journal of Traditional Chinese Medicine 2024年第3期44卷 609-619页

作者： WANG Yuhuang zhang Le zhang Zhengshan YAO Zhi LI Xiyao SUN Luying LIAO Xing Department of Nephropathy and Endocrine Dongzhimen HospitalBeijing University of Chinese MedicineBeijing 100010China Centre for Evidence-Based Chinese Medicine Institute of Basic Research in Clinical MedicineChina Academy of Chinese Medical SciencesBeijing 100010China Department of Endocrine One Zone Fangshan HospitalBeijing University of Chinese MedicineBeijing 102400China

OBJECTIVE:To assess the quality of Clinical practice guidelines(CPGs)in the context of diabetic kidney disease(DKD)and determine whether any factors affect the ***:We searched eight databases along with five international and national organizations to develop or archive guidelines from their inception to July 2023,with an additional search of *** the authoritative organizations related to *** and consensus statements created using direct differential diagnosis or therapy for DKD were included without language *** quality was evaluated by four reviewers using the Appraisal of Guidelines for Research and EvaluationⅡ(AGREEⅡ)*** with the item and domain scores,the guideline was also allocated an overall quality score,which ranged from 1(lowest possible quality)to 7(highest possible quality).Moreover,an overall recommendation for use was also assigned(“recommended”,“recommended with modifications”or“not recommended”).RESULTS:A total of 16 CPGs were included,of which 14 were from Asia and the remaining two from *** two CPGs were updated in the third *** CPGs were recommended for use because their primary domains scored in the medium or high ***,five CPGs were recommended with modifications as the stakeholder involvement,applicability,and editorial independence domains were evaluated as low *** all domains,the lowest average score was for rigour of development(33%),followed by application(36%),and stakeholder involvement(51%).The highest average score was for scope and purpose(79%),followed by clarity of presentation(75%).None of the CPGs considered the patient's viewpoint,and six of 16 CPGs did not use any grading system to translate the evidence into ***,only three of 16 CPGs shared search strategy,and eight of 16 CPGs did not declare a funding ***:According to the AGREE II evaluation,more than one in four CPGs for DKD had poo

关键词： diabetic nephropathies practice guideline appraisal of guidelines for research and evaluation II quality assessment systematic review

Determination of S-propargyl-cysteine in rat plasma by mixed-mode reversed-phase and cation-exchange HPLC-MS/MS method and its application to pharmacokinetic studies

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Determination of S-propargyl-cysteine in rat plasma by mixed...

Culture of Bovine Fetal Skin Fibroblasts in vitro and Ipr1 Gene Transfection

第十二次全国临床药理学学术会议

作者： Yuanting Zheng~1,Hongrui Liu~(2,a),Guo Ma~1,Ping Yang~3,Lin zhang~1,Yi Gu~4,Qing zhu~2, Tengfei Shao~1,Peng zhang~1,Yizhun zhu~(2,*),Weimin Cai~1 (1 Department of Clinical Pharmacy,school of Pharmacy,Fudan university,Shanghai,china 2 Department of Pharmacology,school of Pharmacy and Institute of Biomedical Sciences,Fudan university,Shanghai,china 3 Analytical and Testing Center,school of Pharmacy,Fudan university,Shanghai,china 4 Drug Metabolism and Pharmacokinetics, Hutchison Medipharma Ltd.,Shanghai,china)

A simple,fast and sensitive mixed-mode reversed-phase and cation-exchange HPLC-MS/MS method for the quantification of S-propargyl-cysteine(SPRC),a novel cardioprotective agent,has been developed and validated for preclinical *** separation of SPRC and its internal standard(IS) was performed using a commercial analytical column which contained both C18 bonded silica particles and sulfonic acid cation-exchange *** optimized mobile phase was composed of acetonitrile/ammonium acetate buffer(10mM,pH=4):30/70(v/v).

关键词：

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畜牧兽医学报 2011年第S1期 7-12页

作者： SONG Yong-li 1,2,HE Xiao-ning 2,HUA Song 2,LAN Jie 2,ZHENG Yue-mao 2,LI Ji-xia 2,HE Xiao-ying 2,QUAN Fu-sheng 2,zhang Yong 2(1. College of Veterinary Medicine,Jilin Agricultural Science and Technology College,Jilin132101,china 2. Key Laboratory of Animal Reproductive Physiology & Embryo Technology,Ministry of Agriculture,College of Veterinary Medicine,Northwest Agricuture and Forestry university,Yangling 712100,china)

In order to generate transgenic donor cells for nuclear transfer, bovine fetal fibroblasts were isolated in vitro and transfected with the eukaryotic expression vector pSRA-EGFP-Ipr1. The mouse Ipr1 gene and human SR-A promoter were successfully cloned and then used to construct this macrophage-specific eukaryotic expression vector. Bovine fetal fibroblasts in stable primary culture (4th passage) were transfected with pSRA-EGFP-Ipr1 by electroporation. Fluorescence from GFP was observed after 24h. Transgenic cells were selected using G418 and the resultant monoclones were picked and expanded. The transgenic cells, at the 9 th passage, were evaluated by PCR and flow cytometry. The inserted Ipr1 was confirmed by PCR, indicating stable integration of the transgene into the genome and cells had normal karyotypes and very good appearance, which indicate no deleterious result of the transgenesis. In conclusion, the cells obtained could be used as donor cells for nuclear transfer for further research of transgenic cattle.

关键词： donor cells eunuclear expression vector Ipr1 gene bovine fetal fibroblasts cells electroporation EGFP

Menstrual Factors,Reproductive Factors and Lung Cancer Risk:A Meta-analysis

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中国肺癌杂志 2012年第12期15卷 701-719页

作者： Yue zhang 1,2,3,Zhihua YIN 1,2,3,Li SHEN 1,2,3,Yan WAN 1,2,3,Baosen ZHOU 1,2,3 1 Department of Epidemiology,school of Public Health,china Medical university,Shenyang 110001,china 2 Key Laboratory of Cancer Etiology and Intervention,Liaoning university,Shenyang 110036,china 3 china Medical university Center For Evidencebased Medicine,Shenyang 110001,china Department of Epidemiology School of Public Health China Medical University Shenyang 110001 China.

Background and objective Epidemiological studies have suggested that menstrual and reproductive factors may in uence lung cancer risk,but the results are *** therefore carried out a meta-analysis aiming to examine the associations of lung cancer in women with menstrual and reproductive *** Relevant studies were searched from PubMed database,CNKI,WANFANG DATA and VIP INFORMATION up to January 2012,with no language *** listed from selected papers were also *** included studies that reported the estimates of relative risks(RRs) with 95% con dence intervals(CIs) for the association between menstrual and reproductive factors and lung cancer risk.e pooled RRs were calculated a er the heterogeneity test with the so ware Stata 11,and publication bias and sensitivity were evaluated at the same *** Twenty-ve articles,representing 24 independent studies,were included in this *** age at menarche in North America women(RR=0.83;95%CI:0.73-0.94) was associated with a signi cant decreased risk of lung *** length of menstrual cycle was also associated with decreased lung cancer risk(RR=0.72;95%CI:0.57-0.90).Other exposures were not signi cantly *** Our analysis provides evidence of the hypothesis that female sex hormones in uence the risk of lung cancer in women,yet additional studies are warranted to extend this nding and to clarify the underlying mechanisms.

关键词：《中国肺癌杂志》医学期刊编辑部编辑工作