检索结果-南通市图书馆

Lactobacillus rhamnosus GR-1 attenuates foodborne Bacillus cereus-induced NLRP3 inflammasome activity in bovine mammary epithelial cells by protecting intercellular tight junctions

在线全文

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2023年第1期14卷 307-321页

作者： Qiang Shan Ning Liu Xue Wang yaohong zhu Jinhua Yin Jiufeng Wang Department of Veterinary Clinical Sciences College of Veterinary MedicineChina Agricultural UniversityBeijing 100193China. College of Animal Science and Technology Tarim UniversityAlar 843300China.

Background:Bacillus cereus is an important pathogen that causes human food poisoning,specifically diarrhea and *** can also induce mastitis in dairy cows and has a strong survival ability in milk,as it cannot be inactivated by high-temperature short-time ***,*** can enter the market through pasteurized milk and other dairy products,imposing enormous hidden dangers on food safety and human ***:In this study,*** 2101(BC)was isolated from milk samples of cows with *** grew rapidly with strong hemolysis,making it difficult to prevent mastitis and ensure food ***-T cells were treated with BC and/or Lactobacillus rhamnosus GR-1(LGR-1).Pretreatment with LGR-1 protected the integrity of tight junctions and the expression of zonula occludens-1(ZO-1)and occludin destroyed by ***,LGR-1 pretreatment reduced the expression of NOD-like receptor family member pyrin domain-containing protein 3(NLRP3),caspase recruitment and activation domain(ASC),Caspase-1 p20,gasdermin D(GSDMD)p30,inflammatory factors(interleukin(IL)-1βand IL-18),and cell death induced by ***,LGR-1 pretreatment reduced NLRP3 inflammasome activity and increased expressions of ZO-1 and occludin induced by lipopolysaccharides(LPS)+ATP ***-T cells were transfected with NLRP3 si RNA or MCC950 and/or treated with BC and/or ***3-si RNA transfection and MCC950 attenuated BC-induced NLRP3 inflammasome *** of inflammatory cytokines and cell death suggested that the inflammatory pathway might play an important role in the induction of the NLRP3 inflammasome by BC and the protection of ***:These results suggest that LGR-1 might be a probiotic alternative to antibiotics and could be administered to prevent mastitis in dairy cows,thus ensuring food security.

关键词： Bacillus cereus Intercellular tight junctions Lactobacillus rhamnosus GR-1 NLRP3 inflammasome

Prepartum body conditions affect insulin signaling pathways in postpartum adipose tissues in transition dairy cows

在线全文

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2019年第3期10卷 648-657页

作者： Fanjian Zhang Dan Li Qiong Wu Jian Sun Wenyi Guan Yinxu Hou yaohong zhu Jiufeng Wang Beijing Vocational College of Agriculture Beijing 102442People’s Republic of China College of Veterinary Medicine China Agricultural UniversityBeijing 100193People’s Republic of China Animal Science and Technology College Beijing University of AgricultureBeijing 102206People’s Republic of China

Background: Overconditioned dairy cows are susceptible to excessive lipolysis and increased insulin resistance during the transition *** associations among body fat reserve,insulin resistance,and lipolysis in adipose tissues(AT) remain to be ***,this study aimed to investigate whether excessive fat reserves influence the insulin signaling pathway in AT ***: Twenty multiparous dairy cows were selected and assigned to one of two groups,according to prepartum body condition score(BCS): Control group(BCS = 3.0–3.5;n = 10) and Overconditioned group(BCS ≥ 4.0;n = 10).Blood samples were collected on days-14,-7,-4,-2,-1,0,1,2,4,7,and 14 relative to *** AT were collected on day 2 following parturition for quantitative real-time polymerase chain reaction and western blot *** differences were observed between the two groups in serum glucose,non-esterified fatty acids,β-hydroxybutyric acid,tumor necrosis factor(TNF)α,insulin,or leptin concentrations during the experimental *** with the control cows,the overconditioned cows had lower serum triglyceride levels and higher adiponectin *** the AT postpartum,insulin receptor mRNA and protein levels were lower in the overconditioned cows than in the control cows,and no differences were found in glucose transporter 4 *** with the control cows,the overconditioned cows had lower mRNA levels of TNFα and higher mRNA levels of peroxisome proliferator-activated receptor gamma(PPARγ) in AT *** phosphorylated protein kinase B(AKT) content and phosphorylation rate of AKT were increased in the overconditioned cows compared with the control cows,which suggested that the downstream insulin signaling in AT was ***: In the present study,transition dairy cows with higher BCS did not show more fat *** changes of insulin signaling pathway in AT postpartum of overconditioned cows may be partly related to the ex

关键词： Adipose tissue Body condition score Insulin signaling pathway Transition dairy cow

Lactobacillus johnsonii ameliorates the enteritis through activating the autophagy and inhibit the activation of NLRP3 inflammasome caused by Salmonella infantis

在线全文

学校读者我要写书评

暂无评论

Lactobacillus johnsonii ameliorates the enteritis through ac...

Epidemiological Analysis of Recurrent Mastitis on a Dairy Farm in Beijing

中国畜牧兽医学会兽医产科学分会第十四次学术研讨会

作者： Bing Xia yaohong zhu College of Veterinary Medicine China Agricultural University

Twenty-four 21-day-old weaned piglets were randomly divided into three groups:(1) control group,oral administration of sterile physiologic saline;(2) Salmonella group,oral administration of sterile

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Epidemiological Analysis of Recurrent Mastitis on a Dairy Fa...

中国畜牧兽医学会兽医产科学分会第十六次学术研讨会

作者： Wang Siyu zhu yaohong College of Veterinary Medicine Chinese Agricultural University

Objective:This study aimed to investigate the epidemiological characteristics and risk factors of mastitis in a dairy farm of Beijing during September to October in 2021,and supply reasonable measures for ***:In this study,milk samples were collected aseptically from cows with recurrent mastitis for bacterial culture and isolation,then PCR sequencing and homologous sequence matching were applied to identify the pathogenic *** conducting on-site hygienic investigation,we examined the descriptive epidemiological characteristics of clinical symptoms and distribution characteristics of the three ***,we analyzed the causes of recurrent morbidity with a retrospective cohort ***:Recurrent mastitis had last for 2 months,with a cumulative total of 53 reported *** to the identification results of bacteria isolated from milk samples,it was found that main pathogenic bacteria were Streptococcus(21/53),Escherichia(9/53),Bacillus(6/53) and Staphylococcus(1/53),of which 16 cases had no bacterial *** with mastitis were mainly concentrated in 2-calving cows(8/45).Results of on-site hygienic investigation suggested that the recurrent mastitis on this dairy farm in September might due to Streptococcal infection and transmission caused by inadequate milking parlour disinfection and milking parlour *** found that the isolation rate of Streptococcal decreased in October after the disinfection of teat *** the retrospective cohort study showed,teat cups without sterilization caused mastitis by Streptococcal infections was 10 times higher(95% CI 2.426-41.221) than disinfection of teat cups,and the incidence was 4.6 times higher(95% CI 1.539-13.749).Thus,effective parlour sterilization is important to prevent and control infectious mastitis.A chi-square analysis and t-test showed that the parity was strongly associated with the incidence of ***:The study investigated recurrent mastitis in a dair

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

宁波市轨道交通建设创新成果与展望

城市轨道交通研究 2018年第5期21卷 51-58页

作者：朱瑶宏宁波市轨道交通集团有限公司宁波315101 宁波大学建筑工程与环境学院宁波315211

宁波轨道交通始建于2009年,作为城市轨道交通建设中的后来者,却在近些年来以管理与技术创新为抓手,研发了类矩形盾构隧道、机械法联络通道等新技术,成为行业的引领者,推动了行业的发展。从宁波轨道交通工程建设阶段的技术发展理念、创... 详细信息

宁波轨道交通始建于2009年,作为城市轨道交通建设中的后来者,却在近些年来以管理与技术创新为抓手,研发了类矩形盾构隧道、机械法联络通道等新技术,成为行业的引领者,推动了行业的发展。从宁波轨道交通工程建设阶段的技术发展理念、创新机制、团队建设,以及对未来的建设规划等角度,对宁波轨道交通的技术创新成果和技术创新体系进行阐述,并对未来宁波轨道交通的技术发展方向进行展望。

关键词：宁波轨道交通建设管理技术创新

在线全文

学校读者我要写书评

暂无评论

内置式泵房在城市轨道交通工程中的创新应用

城市轨道交通研究 2018年第8期21卷 12-16页

作者：朱瑶宏宁波市轨道交通集团有限公司宁波315101 宁波大学建筑工程与环境学院宁波教授级高级工程师315211

城市轨道交通区间隧道废水泵房与联络通道普遍采用合并建设的方式。国内软土地区的联络通道(兼废水泵房)目前多采用冻结法+矿山法施工,废水泵房需在联络通道开挖完成后,在其底部往下开挖3~5 m,施工风险很大。结合宁波市轨道交通工程,设... 详细信息

城市轨道交通区间隧道废水泵房与联络通道普遍采用合并建设的方式。国内软土地区的联络通道(兼废水泵房)目前多采用冻结法+矿山法施工,废水泵房需在联络通道开挖完成后,在其底部往下开挖3~5 m,施工风险很大。结合宁波市轨道交通工程,设计了盾构区间内置式泵房,并详细介绍了泵房的总体布置和相关专业的设计方案。该设计能够满足废水泵房的使用功能,同时满足现行设计规范的要求。内置式泵房的泵房位置从区间隧道外调整至区间隧道内,大大减小了联络通道的体量,从而减小了区间隧道的施工风险。同时,泵房与联络通道分离,使得联络通道的平面位置选择和区间纵断面的设计更为灵活。

关键词：城市轨道交通内置式泵房联络通道区间隧道

宁波市轨道交通1号线高性能混凝土质量控制技术

在线全文

学校读者我要写书评

暂无评论

施工技术 2013年第4期42卷 38-40,58页

作者：朱瑶宏宁波市轨道交通工程建设指挥部浙江宁波315012

针对国内地铁工程施工中常出现的混凝土质量控制问题,宁波市轨道交通建设工程基于工程结构特点和使用环境,通过以甲方供应方式公开招标水泥和粗骨料、对商品混凝土进行公开招标、设立第三方试验检测中心、加强配合比报批管理、开展混凝... 详细信息

针对国内地铁工程施工中常出现的混凝土质量控制问题,宁波市轨道交通建设工程基于工程结构特点和使用环境,通过以甲方供应方式公开招标水泥和粗骨料、对商品混凝土进行公开招标、设立第三方试验检测中心、加强配合比报批管理、开展混凝土性能专题研究等措施控制混凝土质量,提高整个工程结构的质量和使用寿命。轨道交通1号线一期工程樱花公园站C35 P8混凝土强度统计和耐久性指标表明,以上措施有效加强了混凝土的质量控制,保证了混凝土的施工质量。

关键词：混凝土轨道交通高性能混凝土质量控制

Antimicrobial Effect of Zophobas morio defensin against Bovine Mastitis Pathogens

在线全文

学校读者我要写书评

暂无评论

Antimicrobial Effect of Zophobas morio defensin against Bovi...

A selected Lactobacillus rhamnosus strain promotes EGFR-independent Akt activation in an enterotoxigenic Escherichia coli K88-infected IPEC-J2 cell model

中国畜牧兽医学会兽医产科学分会第八届会员代表大会暨第十五次学术研讨会

作者： WANG Xue LI Shuxian DU Mengze WANG Jiufeng zhu yaohong College of Veterinary Medicine China Agricultural University

Objective:Mastitis has always been considered as a great threat for dairy cow industry around the ***,mastitis treatment mainly relies on ***,many countries restrict the use of antibiotics since the bacterial resistance and drug residues have become a serious issue in the past decades of ***,it is urgent to develop a broad-spectrum,low residual anti-bacterial drug against bacterial resistance and drug ***:We analyzed the active ingredient of Zophobas morio(***) hemolymph and obtained the full-length complete coding sequence(CDs) of *** defensin through 5’ and 3’ RACE *** target gene was cloned into pGEX 6 p-1 and transformed into *** Rosetta(DE3) *** protein expression was induced with isopropyl-p-D-1-thiogalactopyranoside(IPTG),and purified through GSTrap 4 ***,the recombinant protein was treated with Prescission Protease and purified by Superdex 30 to remove glutathione S-transferase(GST)-***-zone assay was carried out to evaluate the antimicrobial activity of recombinant *** assay and cytotoxicity test were used to evaluate the safety of recombinant ***:Our results showed that the gene of Z. morio defensin encodes 89 amino acids,and the structure of *** defensin protein includes a conformationally flexible amino-terminal loop(n-loop) followed by a cysteinestabilized α-helical and β-sheet(CSαβ) domain,which is a structural scaffold shared by defensins of other *** addition,recombinant Z. morio defensin protein could inhibit multiple bacteria growths in vitro,such as Escherichia coli,Staphylococcus aureus,Klebsiella pneumonia,bacillus cereus,et ***,recombinant Z. morio defensin protein also maintains non-hemolytic and non-cytotoxicity as ***:In summary,recombinant Z. morio defensin protein which showed excellent antibacterial activity and safety,is a promising drug candidate to treat mastitis for dairy cows.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

畜牧与饲料科学 2015年第Z1期 27-42页

作者： ZHANG Wei zhu yaohong YANG Jincai YANG Guiyan ZHOU Dong WANG Jiufeng Department of Veterinary Clinical Sciences College of Veterinary Medicine China Agricultural University

Enterotoxigenic Escherichia coli（ETEC） are important intestinal pathogens that cause diarrhea in humans and *** probiotic bacteria may protect against ETEC-induced enteric infections,the underlying mechanisms are *** this study,porcine intestinal epithelial J2 cells（IPEC-J2） were pre-incubated with and without Lactobacillus rhamnosus ATCC 7469 and then exposed to F4+ *** in TLR4 and NOD2 mRNA expression were observed at 3 h after F4+ ETEC challenge,but these increases were attenuated by *** *** of TLR2 and NOD1 mRNA was upregulated in cells pre-treated with ***-treatment with *** counteracted F4+ ETEC-induced increases in TNF-α*** *** were observed in cells infected with F4+ ETEC and in cells treated with *** only.A decrease in phosphorylated epidermal growth factor receptor（EGFR） was observed at 3 h after F4+ ETEC challenge in cells treated with ***-treatment with *** enhanced Akt phosphorylation and increased ZO-1 and occludin protein *** findings suggest that *** protects intestinal epithelial cells from F4+ ETEC-induced damage,partly through the anti-inflammatory response involving synergism between TLR2 and *** addition,*** promotes EGFR-independent Akt activation,which may activate intestinal epithelial cells in response to bacterial infection,in turn increasing tight junction integrity and thus enhancing the barrier function and restricting pathogen ***-incubation with *** was superior to co-incubation in reducing the adhesion of F4+ ETEC to IPEC-J2 cells and subsequently attenuating F4+ ETEC-induced mucin layer destruction and suppressing *** data indicate that a selected *** strain interacts with porcine intestinal epithelial cells to maintain the epithelial barrier and promote intestinal epithelial cell activation in response to bacterial infection,thus

关键词： ETEC porcine mucin incubation destruction diarrhea incubated maintain tight infected