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Acta Oceanologica Sinica 2019年第2期38卷 96-106页

作者： yanbing zhu He Gao Hebin Li Hui Ni Zedong Jiang Lijun Li Anfeng Xiao College of Food and Biological Engineering Jimei University Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering Research Center of Food Biotechnology of Xiamen City Key Laboratory of Systemic Utilization and In-depth Processing of Economic Seaweed Xiamen Southern Ocean Technology Center of China Department of Pharmacy Xiamen Medical College

An agarase gene containing 1 302 bp was cloned from Microbulbifer sp. AG1. It encoded a mature protein of 413 amino acids plus a 20-residue signal peptide. The recombinant enzyme without the signal peptide was expressed and purified from Escherichia coli BL21(DE3). When agarose was used as a substrate, the optimal temperature and pH for the enzyme were 60℃ and 7.5, respectively. The recombinant agarase showed excellent thermostability with 67% and 19% of residual activities after incubation at 50℃ and 60℃ for 1 h, *** SDS, the recombinant agarase had a relatively good resistance against the detected inhibitors, detergents and urea denaturant. Thin layer chromatography analysis and enzyme assay using p-nitrophenyl-α/β-Dgalactopyranoside revealed that the recombinant agarase was a β-agarase that degraded agarose into neoagarotetraose as the main end product. The enzymatic hydrolysis products with different degree of polymerization exhibited the antioxidant activities.

关键词： thermostable β-agarase neoagarotetraose Microbulbifer sp.

Propofol Attenuates a-Synuclein Aggregation and Neuronal Damage in a Mouse Model of Ischemic Stroke

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Neuroscience Bulletin 2020年第3期36卷 289-298页

作者： Yuzhu Wang Dan Tian Changwei Wei Victoria Cui Huan Wang yanbing zhu Anshi Wu Yun Yue Department of Anesthesiology Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China Experimental and Translational Research Center Beijing Friendship HospitalCapital Medical UniversityBeijing 100050China Washington University School of Medicine St.LouisMIUSA

a-Synuclein is a soluble monomer abundant in the central nervous system. Aggregates of a-synuclein,consisting of higher-level oligomers and insoluble fibrils,have been observed in many chronic neurological diseases and are implicated in neurotoxicity and neurodegeneration.a-Synuclein has recently been shown to aggregate following acute ischemic stroke, exacerbating neuronal *** is an intravenous anesthetic that is commonly used during intravascular embolectomy following acute ischemic stroke. While propofol has demonstrated neuroprotective properties following brain injury, the mechanism of protection in the setting of ischemic stroke is unclear. In this study, propofol administration significantly reduced the neurotoxic aggregation of a-synuclein, decreased the infarct area, and attenuated the neurological deficits after ischemic stroke in a mouse model. We then demonstrated that the propofol-induced reduction of a-synuclein aggregation was associated with increased mammalian target of rapamycin/ribosomal protein S6 kinase beta-1 signaling pathway activity and reduction of the excessive autophagy occurring after acute ischemic stroke.

关键词： Propofol a-Synuclein Autophagy Stroke Neuroprotection

Substitution of His260 residue alters the thermostability of Pseudoalteromonas carrageenovora arylsulfatase

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Acta Oceanologica Sinica 2019年第6期38卷 75-82页

作者： yanbing zhu Xiaoqian Yin Han Liu Hebin Li Yanhong Chen Lijun Li Anfeng Xiao Hui Ni College of Food and Biological Engineering Jimei UniversityXiamen 361021China Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering Xiamen 361021China Research Center of Food Biotechnology of Xiamen City Xiamen 361021China Key Laboratory of Systemic Utilization and In-depth Processing of Economic Seaweed Xiamen Southern Ocean Technology Center of ChinaXiamen 361021China Department of Pharmacy Xiamen Medical CollegeXiamen 361023China

This study aimed to improve the thermostability of arylsulfatase from Pseudoalteromonas carrageenovora. A library of P. carrageenovora arylsulfatase mutants was constructed by introducing random mutagenesis using error-prone PCR. After screening, two mutants of H260L and D84A/H260L showed enhanced thermal stability than the wild-type predecessor (WT). Site-directed mutagenesis demonstrated that only amino acid residue at Position 260 plays an important role in the thermostability of P. carrageenovora arylsulfatase. Thermal inactivation analysis showed that the half-life (t1/2) values at 55°C for H260L, H260I, H260Q, H260F and H260R were 40.6, 48.4, 30.9, 29.1 and 34.5 min, respectively, while that of WT was 9.1 min. Structure modeling demonstrated that the additional hydrogen bonds and/or optimization of surface charge-charge interactions could be responsible for the increased thermostability imparted by H260L, H260I, H260Q, H260F and H260R.

关键词： arylsulfatase Pseudoalteromonas carrageenovora directed evolution error-prone PCR thermostability

Characterization of a thermostable manganese-containing superoxide dismutase from inshore hot spring thermophile Thermus ***1

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Acta Oceanologica Sinica 2011年第6期30卷 95-103页

作者： zhu yanbing LI Hebin ZHANG Xuqin ZHANG Chunyan XIANG Jionghua LIU Guangming School of Biotechnology Engineering Jimei University Xiamen 361021 China Xiamen Medical College Xiamen 361008 China

A thermostable superoxide dismutase （SOD） from the inshore thermophile Thermus sp. JM1 was purified to homogeneity by steps of fractional ammonium sulfate precipitation, DEAE-Sepharose chromatography and Phenyl-Sepharose chromatography. The specific activity of the purified native enzyme was 1 656 U/mg. A sod gene from this strain was cloned and overexpressed in Escherichia coli （E. coli）. The prepared apo-enzyme of the purified recombinant SOD （rSOD） was reconstituted with either Fe or Mn by means of incubation with appropriate metal salts. As a result, only Mn 2＋ - reconstituted rSOD （Mn-rSOD） exhibited the specific activity of 1 598 U/mg. SOD from Thermus sp. JM1 was Mn-SOD, judging by the specific activities analysis of Fe or Mn reconstituted rSODs and the insensitivity of the native SOD to both cyanide and H 2 O 2 . Both the native SOD and Mn- rSOD were determined to be homotetramers with monomeric molecular mass of 26 kDa and 27.5 kDa, respectively. They had high thermostability at 50 ° C and 60 ° C, and showed striking stability across a wide pH span from 4.0 to 11.0.

关键词： manganese superoxide dismutase thermostability purification reconstitution

Propofol Protects Regulatory T Cells, Suppresses Neurotoxic Astrogliosis, and Potentiates Neurological Recovery After Ischemic Stroke

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Neuroscience Bulletin 2021年第5期37卷 725-728页

作者： Yuzhu Wang Dan Tian Yushang Zhao Mengyao Qu Yuhualei Pan Changwei Wei yanbing zhu Anshi Wu Department of Anesthesiology Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China Experimental and Translational Research Center Beijing Friendship HospitalCapital Medical UniversityBeijing 100050China

Dear Editor,Emergency intravascular thrombectomy is the best treatment for acute ischemic stroke [1]. However, it is controversial whether the use of general anesthesia during emergency intravascular thrombectomy affe... 详细信息

关键词： anesthesia acute intravascular

A weakly-solvated ether-based electrolyte for fast-charging graphite anode

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Chinese Chemical Letters 2024年第8期35卷 526-532页

作者： Xiao zhu yanbing Mo Jiawei Chen Gaopan Liu Yonggang Wang Xiaoli Dong Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials Institute of New EnergyiChEM(Collaborative Innovation Center of Chemistry for Energy Materials)Fudan UniversityShanghai 200433China

Weakly-solvated electrolytes(WSEs)utilizing solvents with weak coordination ability offer advantages for low-potential graphite anode owing to their facile desolvation process and anions-derived inorganic-rich solid electrolyte interphase(SEI)***,these electrolytes face challenges in achieving a balance between the weak solvation affinity and high ionic conductivity,as well as between rigid inorganic-rich SEI and flexible SEI for long-term ***,we introduce 1,3-dioxolane(DOL)and lithium bis(trifluoromethanesulfonyl)-imide(LiTFSI)as functional additives into a WSE based on nonpolar cyclic ether(1,4-dioxane).The well-formulated WSE not only preserves the weakly solvated features and anion-dominated solvation sheath,but also utilizes DOL to contribute organic species for stabilizing the SEI *** from these merits,the optimized electrolyte enables graphite anode with excellent fast-charging performance(210 mAh/g at 5 C)and outstanding cycling stability(600 cycles with a capacity retention of 82.0%at room temperature and 400 cycles with a capacity retention of 80.4%at high temper-ature).Furthermore,the fabricated LiNi_(0.8)Co_(0.1)Mn_(0.1)O_(2)||graphite full cells demonstrate stable operation for 140 cycles with high capacity retention of 80.3%.This work highlights the potential of tailoring solvation sheath and interphase properties in WSEs for advanced electrolyte design in graphite-based lithium-ion batteries.

关键词： Weakly-solvated solvent Bisalt ether-based electrolyte Graphite anode NCM||graphite battery Interfacial optimization

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新建桥涵上跨运营隧道保护方案及措施研究

铁道工程学报 2021年第12期38卷 102-107页

作者：朱炎兵中铁第四勘察设计院集团有限公司武汉430063

研究目的:运营地铁隧道正上方作为轨道交通的特别保护区,为了减少对下卧隧道的影响,上方拟建项目设计方案多工序繁复、造价高且施工工期长。本文通过对某运营隧道上方新建桥涵工程设计方案和施工过程的综合研究,拟找到一种工艺简单、工... 详细信息

研究目的:运营地铁隧道正上方作为轨道交通的特别保护区,为了减少对下卧隧道的影响,上方拟建项目设计方案多工序繁复、造价高且施工工期长。本文通过对某运营隧道上方新建桥涵工程设计方案和施工过程的综合研究,拟找到一种工艺简单、工程费用低,施工快捷且对地铁保护较好的解决方案。研究结论:(1)基于基坑卸载回弹相关研究和时空效应原理,新建桥涵采用预制箱涵拼装方案,土体小块开挖、结构分块快速吊装回筑,施工过程配重快速动态荷载平衡等措施,可以减少隧道上方土体应力变化幅度、范围以及变化的时间跨度,有利于控制对下卧地铁隧道结构的变形影响;(2)数值分析结果及实测数据表明,设计和施工统筹考虑的轨道保护方案对隧道变形的控制较好;(3)研究案例实施快捷、工程造价低、卸载再加载过程对地铁影响较小,可为其他上穿隧道工程的设计和施工提供借鉴和参考。

关键词：桥涵基坑工程地铁隧道上跨保护措施

Revisiting the sodium-ion storage capability of hard carbon in carbonate-based electrolytes via a sodium-metal-free protocol

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Science China Chemistry 2024年第7期67卷 2240-2247页

作者： Gaopan Liu yanbing Mo Jiawei Chen Yu Peng Xiao zhu Fei Wang Xiaoli Dong Yongyao Xia Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials Institute of New EnergyLaboratory of Advanced MaterialsFudan UniversityShanghai 200438China

Common evaluation methodology of sodium(Na)-containing two-electrode or three-electrode configurations overlooks the interference from highly reactive Na metal,leading to the underestimation or inconsistent performance of low-potential hard carbon(HC)***,the trap of Na metal was systematically investigated with or without applied current,uncovering its inadequacy as the reference or counter electrode in different configurations.A Na-metal-free three-electrode protocol is proposed for evaluating the actual Na^(+)-storage capability of the typical low-potential HC *** avoiding Na crosstalk and precisely controlling the working electrode's potential,the actual electrochemical performance of HC in the carbonate esterbased electrolyte can be recognized with high capacity of 222 mAh g^(-1)at 2 C and 113 mAh g^(-1)at 5 C,correcting the misunderstanding of the inferior performance of HC in coin-type half cells(68%and 50%undervaluation at 2 C and 5 C,respectively).The advanced protocol is expected to reduce misunderstandings or underestimation due to evaluation methods and to guide the development of high-performance battery materials.

关键词： highly reactive sodium potential drift carbonate ester sodium-metal-free protocol sodium storage capability

Characterization of chondroitinase 1816 from Microbulbifer sp. ALW1

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Characterization of chondroitinase 1816 from Microbulbifer s...

中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： Liang Meifang zhu yanbing College of Food and Biological Engineering

The gene of chondroitinase 1816(ChSase1816) was overexpressed and purified from Escherichia coli BL21(DE3) with a molecular mass of 86 *** the chondroitin sulfate B(CS-B) as the substrate,the Km and Vmax values of ChSase1816 towards CS-B was 7.85 μg/mL and 1.21 U/*** optimum reaction temperature and pH for ChSase1816 were 30℃ and 8.0,*** ChSase1816 activity was stable at 30°C,but not stable at 50°C and 60°***,ChSase1816 retained more than 60% of its maximum activity at a broad pH range of pH 4.0 and *** the metal ions detected had inhibitory effects on the *** result of Circular dichroism(CD) indicated the enzyme contained many antiparallel structure and random *** antioxidant activity assay showed that hydroxyl radical scavenging activity of chondroitin enzymatic hydrolysates was higher than chondroitin sulfate.

关键词： Chondroitinase Enzymatic properties Enzymatic hydrolysate

来源： cnki会议评论

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白芽奇兰茶味复配胶软糖的研制

集美大学学报（自然科学版） 2024年第2期29卷 119-125页

作者：石亚芳许玲玲林坤城洪清林林庆祥康自强姜泽东倪辉朱艳冰集美大学海洋食品与生物工程学院福建厦门361021 福建省食品微生物与酶工程重点实验室福建厦门361021 绿新(福建)食品有限公司福建漳州363107

为研究κ-卡拉胶与魔芋胶复配凝胶剂在软糖中的应用,通过质构分析和感官评价,确定软糖中复配凝胶剂的比例;综合白芽奇兰茶粉添加量、麦芽糖浆添加量、白砂糖添加量、复合胶添加量为考察因素,以感官评分为考察指标,通过单因素及正交实验... 详细信息

为研究κ-卡拉胶与魔芋胶复配凝胶剂在软糖中的应用,通过质构分析和感官评价,确定软糖中复配凝胶剂的比例;综合白芽奇兰茶粉添加量、麦芽糖浆添加量、白砂糖添加量、复合胶添加量为考察因素,以感官评分为考察指标,通过单因素及正交实验确定软糖的最佳配方。结果表明:白芽奇兰茶粉添加量质量分数为0.3%,麦芽糖浆添加量质量分数为40%,白砂糖添加量质量分数为25%,复合胶添加量质量分数为2.4%,在此条件下,所制得的软糖的感官评分为78.1分。软糖咀嚼性和弹性较好,并具有良好茶香味。

关键词： κ-卡拉胶魔芋胶软糖白芽奇兰茶粉