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Melittin analog p5RHH enhances recombinant adeno-associated virus transduction efficiency

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Journal of Integrative Medicine 2024年第1期22卷 72-82页

作者： Jing-shun Meng Yun He heng-bin Yang li-ping zhou Si-yuan wang Xi-lin Feng Omar Yahya Al-shargi Xiao-min Yu li-qing Zhu Chang-quan ling Shanghai University of Traditional Chinese Medicine Shanghai 201203China Oncology Department of Traditional Chinese Medicine Changhai HospitalNaval Medical UniversityShanghai 200433China Faculty of Traditional Chinese Medicine Naval Medical UniversityShanghai 200043China State Key Laboratory of Genetic Engineering and Engineering Research Center of Gene Technology(ministry of Education) School of Life SciencesZhongshan HospitalFudan UniversityShanghai 200438China Yantai Fuheng Biological Technology Co. Ltd.YantaiShandong Province 264006China. Department of Clinical Laboratory the First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000Zhejiang ProvinceChina

Objective Melittin and its derivatives have been characterized to establish effective gene delivery *** capability of facilitating endosomal release enhances the nanoparticles-based gene ***,little investigation has been conducted to explore its potential application in the context of viral *** Various melittin-derived peptides were inserted into the loop VIII of the capsid proteins of recombinant adeno-associated virus *** vectors carrying either gfp or fluc genes were subjected to qPCR assays and transduction assays of HEK293T cells to investigate the efficiency of vector production and gene *** addition,the ability of a specific p5RHH-rAAV vector to deliver genes was examined through in vitro transduction of different cultured cells and in vivo tail vein administration to C57BL/6 ***,the intricate details of the vector-mediated transduction mechanisms were revealed by specific pharmacological inhibitors of every stage of the rAAV2 intracellular life *** A total of 76 melittin-related peptides were compiled from existing *** them,cMA2,Melt13,p5RHH and aAR3 were found to significantly enhance the gene delivery efficiency of rAAV2 *** p5RHH-rAAV2 vectors efficiently transduced not only rAAV-potent cell lines but also cell lines previously considered resistant to ***,bafilomycin A1,a vacuolar endosome acidification inhibitor,completely inhibited the transgene expression mediated by the p5RHH-rAAV2 *** importantly,p5RHH-rAAV8 vectors also demonstrated increased hepatic transduction in vivo in C57BL/6 *** The incorporation of melittin analogues into the rAAV capsids results in a significant improvement in rAAV-mediated transgene *** further modifications remain an area of interest,our studies have substantially broadened the pharmacological prospects of melittin in the context of viral vector-mediated gene delivery.

关键词： Melittin Recombinant adeno-associated virus Capsid engineering Transduction efficiency

Scintillation Arc from FRB 20220912A

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Science China(Physics,Mechanics & Astronomy) 2024年第1期67卷 134-142页

作者： Zi-Wei Wu Robert AMain Wei-Wei Zhu Bing Zhang Peng Jiang Jia-Rui Niu Jin-lin Han Di li Ke-Jia Lee Dong-Zi li Yuan-Pei Yang Fa-Yin wang Rui Luo Pei wang Chen-Hui Niu heng Xu Bo-Jun wang Wei-Yang wang Yong-Kun Zhang Yi Feng De-Jiang zhou Yong-Hua Xu Can-min Deng Yu-Hao Zhu National Astronomical Observatories Chinese Academy of SciencesBeijing 100101China Max-Planck-Institut für Radioastronomie Bonn 53121Germany Institute for Frontier in Astronomy and Astrophysics Beijing Normal UniversityBeijing 102206China Nevada Center for Astrophysics and Department of Physics and Astronomy University of NevadaLas VegasNV 89154USA CAS Key Laboratory of FAST National Astronomical ObservatoriesChinese Academy of SciencesBeijing 100101China School of Astronomy University of Chinese Academy of SciencesBeijing 100049China NAOC-UKZN Computational Astrophysics Centre University of KwaZulu-NatalDurban 4000South Africa Department of Astronomy Peking UniversityBeijing 100871China Kavli Institute for Astronomy and Astrophysics Peking UniversityBeijing 100871China Cahill Center for Astronomy and Astrophysics California Institute of TechnologyPasadena 91125USA South-Western Institute For Astronomy Research Yunnan UniversityKunming 650504China School of Astronomy and Space Science Nanjing UniversityNanjing 210093China Key Laboratory of Modern Astronomy and Astrophysics(Nanjing University) Ministry of EducationNanjing 210023China CSIRO Space and Astronomy Epping 1710Australia Department of Astronomy School of Physics and Materials ScienceGuangzhou UniversityGuangzhou 510006China Research Center for Intelligent Computing Platforms Zhejiang LaboratoryHangzhou 311100China Yunnan Observatories Chinese Academy of SciencesKunming 650011China Guangxi Key Laboratory for Relativistic Astrophysics Department of PhysicsGuangxi UniversityNanning 530004China

We present the interstellar scintillation analysis of fast radio burst(FRB)20220912A during its extremely active episode in 2022using data from the Five-hundred-meter Aperture Spherical Radio Telescope(FAST).We detect a scintillation arc in the FRB’s secondary spectrum,which describes the power in terms of the scattered FRB signals’time delay and Doppler *** arc indicates that the scintillation is caused by a highly localized *** analysis favors a Milky Way origin of the ionized interstellar medium(IISM)for the localized scattering medium but cannot rule out a host galaxy *** present our method for detecting the scintillation arc,which can be applied generally to sources with irregularly spaced bursts or *** methods could help shed light on the complex interstellar environment surrounding the FRBs and in our Galaxy.

关键词： fast radio burst scintillation FRB 20220912A

Status of aquatic organisms resources and their environments in Yangtze River system(2017-2021)⋆

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Aquaculture and Fisheries 2024年第5期9卷 833-850页

作者： Haile Yang li Shen Yongfeng He Huiwu Tian Lei Gao Jinming Wu Zhigang Mei Nian Wei lin wang Tingbing Zhu Feifei Hu Jinling Gong Hongchun Du Xinbin Duan Huatang Deng Daoqun wang Fengyue Zhu Yunfeng li Fan Wu Huijun Ru Yan Zhang Junyi li Junlin Yang Yuntao zhou Dongdong Fang Yinping wang Danqing lin Yanping Yang Peijie li Silei liu Jian Yang Ping Zhuang Sikai wang Tao Zhang Gang Yang Wenbo Yang lilai Yuan Kun Cao Shuo Xu Huiyuan liu Zhiqiang liang Chongrui wang Hong li Xiping Yuan Xin Yang Yilong Fu Yanping Zhang Haixin Zhang Zhiying Tao Sheng wang Xiaoping Gao Binsong Jin Kemao li Guojie wang Shenglong Jian Yingqin li Chenjiang Xue Chunyun Lei Shaowei Xue Yi Sun Bin Zhu Ke Shao Xingkun Hu Meihua Xiong Jun Du Bin He Tao Yan Yingying Huang Yuanchao Zou Biwen Xie Yongming wang Bin li Fei liu Yaoyao Zhang Fei Fan Zhijian wang Jing Huang Haoran Gu Hailong Ge Yan Dan Yan li Shuqiao wang Chuang Zhang Lu zhou Xue wang Sheng Zeng Yan Xiang Xugang He Jianhui Qin Chengxing Xia Jie Hou Yifu Shi lifang Gao Zhiqiang Zhu Hongbao Shen Yun Du Xuejun Duan Jiawu Xiong Deguo Yang Shaoping liu Zhaohui Ni Hui Zhang Kai liu Feng Zhao Yingren li Jianwei wang Qiwei Wei Yangtze River Fisheries Research Institute Chinese Academy of Fishery SciencesWuhan430223China Institute of Hydrobiology Chinese Academy of SciencesWuhan430072China Fishery Resource and Environment Research Center Chinese Academy of Fishery SciencesBeijing100141China Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization Ministry of Agriculture and Rural AffairsFreshwater Fisheries Research CenterChinese Academy of Fishery SciencesWuxi214081China East China Sea Fisheries Research Institute Chinese Academy of Fishery SciencesShanghai200090China Institute of Fisheries Engineering Chinese Academy of Fishery SciencesBeijing100141China Hunan Fisheries Science Institute Changsha410153China Jiangxi Fisheries Research Institute Nanchang330039China Jiujiang Academy of Fishery Sciences Jiujiang332000China School of life Sciences Nanchang UniversityNanchang330031China Qinghai Provincial Fishery Technology Extension Center Xining810012China Yunnan Academy of Fishery Sciences Kunming650111China Institute of Hydroecology Ministry of Water Resources and Chinese Academy of SciencesWuhan430079China Fisheries Institute Sichuan Academy of Agricultural SciencesChengdu611731China School of life Sciences Neijiang Normal UniversityNeijiang641100China School of life Sciences Southwest UniversityChongqing400715China Chongqing Fishery Sciences Research Institute Chongqing401120China Guizhou Fisheries Research Institute Guiyang550025China College of Fisheries Huazhong Agricultural UniversityWuhan430072China Hubei Fisheries Science Research Institute Wuhan430070China Shaanxi Fisheries Research Institute Xi’an710086China Innovation Academy for Precision Measurement Science and Technology Chinese Academy of SciencesWuhan430077China Nanjing Institute of Geography and limnology Chinese Academy of SciencesNanjing210008China Central South Inventory and Planning Institute of State Forestry and Grassland Administration Changsha410014China

The Yangtze River is the mother river of *** promote the aquatic ecosystem protection of the great river,the Project of Yangtze Fisheries Resources and Environment Investigation(2017-2021)supported by the ministry of Agriculture and Rural Affairs,*** carried out by 24 institutes and universities that located in the Yangtze River basin surveys the status of(1)fish species composition and spatial distribution,(2)current fish abundance,(3)endangered fishes,(4)Yangtze finless porpoise,(5)aquatic eco-environments,(6)water-level fluctuation areas,(7)capture fisheries and recreational fisheries of the Yangtze River mainstream and 10 of its main tributaries,including Yalong River,heng River,min River(including Dadu River),Chishui River,Tuo River,Jialing River,Wu River,Han River,Dongting Lake and Poyang *** results showed that there were 443 fish species(belonging to 163 genus,37 families,and 18 orders)before 2017,but only 323 fish species(including 15 new recorded exotic species)were recorded in the project of *** them,Cyprinus carpio,Carassius auratus,Hypophthalmichthys molitrix,Pelteobagrus fulvidraco,Coilia brachygnathus,Silurus asotus,Saurogobio dabryi,Ctenopharyngodon idellus,Pelteobagrus nitidus,Hemiculter leucisculus,Siniperca chuatsi,Coreius heterodon,Culter alburnus,Parabramis pekinensis,and Aristichthys nobilis were the dominant species across the whole Yangtze River *** is estimated that there were 886 million individuals weighing 124.8 million kg,merely equivalent to 27.3%of the resources in 1950s,30.9%of the resources in 1960s,or 58.7%of the resources in *** the new list of protected fishes that recorded in the Yangtze River system,only 15 of 29 were collected in this project of *** gladius has been affirmed to be Extinct by International Union for Conservation of Nature(IUCN).The wild individuals of Tenualosa reevesii and Luciobrama macrocephalus have disappeared for many years and maybe have been extinct

关键词： Fishes Yangtze finless porpoise Resources Aquatic ecosystem Fisheries Background investigation Yangtze river system

含酚类煤化工废水自还原Fe^(3+)类芬顿体系研究

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工程科学与技术 2024年第4期56卷 57-65页

作者：丛伯一刘杨殷浩翔张恒周鹏李伟赖波四川大学建筑与环境学院四川成都610065 四川大学化学工程学院四川成都610065 中国五冶集团有限公司四川成都610063

煤化工废水水质复杂,难降解有机物及氨氮含量高,给废水处理带来较大难度。现有的煤化工废水处理技术(混凝法、吸附法、膜生物反应法等)具有成本高、运行不稳定、预处理效果差等缺陷,难以满足煤化工行业发展的需要。为了高效处理煤化工废... 详细信息

煤化工废水水质复杂,难降解有机物及氨氮含量高,给废水处理带来较大难度。现有的煤化工废水处理技术(混凝法、吸附法、膜生物反应法等)具有成本高、运行不稳定、预处理效果差等缺陷,难以满足煤化工行业发展的需要。为了高效处理煤化工废水,本文利用煤化工废水中酚类有机物的还原性促进Fe^(3+)/Fe^(2+)的循环,提出了一种利用Fe^(3+)/H_(2)O_(2)类芬顿体系处理煤化工废水的方法。实验结果表明:Fe^(3+)/H_(2)O_(2)体系中COD、TOC、TN、NH3-N的去除率可以达到74.63%、52.62%、10.46%、15.11%;相比于其他体系,Fe^(3+)/H_(2)O_(2)体系出水色度明显降低,UV-Vis光谱下降幅度最大,铁泥量也明显减少。Q-TOF分析结果表明:废水中主要的8种有机物为酚类或具有醛基、羰基、羧基、碳碳双键或者酯基等还原性的官能团。通过测定COD去除率和pH、Fe^(3+)/Fe^(2+)、H_(2)O_(2)等含量随时间的变化趋势,提出了Fe^(3+)/H_(2)O_(2)体系去除有机物的机理:废水中的还原性有机物将Fe^(3+)还原为Fe^(2+),促进Fe^(3+)/Fe^(2+)循环,生成的Fe^(2+)与H_(2)O_(2)发生芬顿反应,实现废水中有机污染物的去除。利用控制变量法,确定了最佳运行工况为:Fe_(2)(SO_(4))_(3)添加量为1.0 g/L、H_(2)O_(2)添加量为50 mmol/L、反应温度为30℃、初始pH为6.8。在此工况下,反应60 min后,煤化工废水的COD、TOC、TN、NH_(3)-N去除效果良好,色度明显降低,BOD_(5)和COD的比值(B/C)从0.17提升至0.47,可生化性大幅提高。本文证实了利用含酚类煤化工废水自还原Fe^(3+)/H_(2)O_(2)体系的可行性,降低了运行成本,可为后续研究及工程应用提供理论基础。

关键词： Fe^(3+) H_(2)O_(2) 类芬顿煤化工废水酚类化合物

蛋白酶体抑制剂MG132对慢性缺氧致小鼠记忆损伤的作用及其机制

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解放军医学杂志 2024年第4期49卷 449-458页

作者：董华平李鹏李晓栩周思敏肖衡谢佳新黄沛吴玉钟志凤陆军军医大学高原军事医学系高原作业医学教研室重庆400038 极端环境医学教育部重点实验室重庆400038 全军高原医学重点实验室重庆400038

目的探讨蛋白酶体抑制剂MG132对慢性缺氧所致小鼠记忆功能损伤的作用及其机制。方法(1)利用低氧工作站构建小鼠中脑多巴胺能神经元MN9D细胞缺氧损伤模型。将MN9D细胞分为常氧组及缺氧12 h、24 h和48 h组,观察缺氧对MN9D细胞中酪氨酸羟化... 详细信息

目的探讨蛋白酶体抑制剂MG132对慢性缺氧所致小鼠记忆功能损伤的作用及其机制。方法(1)利用低氧工作站构建小鼠中脑多巴胺能神经元MN9D细胞缺氧损伤模型。将MN9D细胞分为常氧组及缺氧12 h、24 h和48 h组,观察缺氧对MN9D细胞中酪氨酸羟化酶(TH)、泛素的K48链(Ub-K48)和Ub-K63表达的影响;将MN9D细胞分为常氧组、缺氧组、缺氧+MG132(25、50、100、200)μmol/L组,观察MG132对缺氧细胞中上述蛋白表达的影响。(2)利用低压舱建立低压缺氧小鼠记忆功能损伤模型。将C57小鼠随机分为常氧组、缺氧3 d组、缺氧21 d组,每组10只,观察低压低氧对小鼠中脑黑质致密部(SNc)TH、Ub-K48和Ub-K63表达的影响;将小鼠随机分为常氧组、缺氧21 d组、缺氧21 d+MG132组,每组8只,观察MG132对缺氧所致小鼠空间记忆损伤的影响。(3)采用Western blotting检测不同缺氧时长及MG132预处理再低氧处理的MN9D细胞中TH、Ub-K48和Ub-K63的蛋白表达水平;采用新物体识别测试检测各组小鼠记忆功能,免疫荧光染色检测SNc区TH阳性免疫反应面积百分比,Western blotting检测小鼠SNc区TH、Ub-K48和Ub-K63表达水平。结果(1)与常氧组比较,缺氧24 h组MN9D细胞中Ub-K48和Ub-K63表达水平增高(P<0.05),TH表达水平降低(P<0.05);各缺氧组Ub-K48/TH和Ub-K63/TH表达水平均增高(P<0.05)。与缺氧组比较,缺氧+MG132100μmol/L组和缺氧+MG132200μmol/L组MN9D细胞中Ub-K48/TH和Ub-K63/TH表达水平降低(P<0.05)。(2)与常氧组比较,缺氧3 d组和缺氧21 d组小鼠中脑SNc区TH表达水平降低(P<0.001),Ub-K48/TH和Ub-K63/TH表达水平升高(P<0.05);缺氧21 d组小鼠新物体识别指数降低(P<0.01),SNc区多巴胺能神经元TH阳性免疫反应面积百分比降低(P<0.05)。与缺氧21 d组比较,缺氧21 d+MG132组小鼠新物体识别指数升高(P<0.01)。结论蛋白酶体抑制剂MG132可改善慢性缺氧所致小鼠记忆损伤,其机制可能与抑制Ub-K48和Ub-K63,上调多巴胺能神经元TH表达有关。

关键词：缺氧记忆损伤蛋白酶体抑制剂酪氨酸羟化酶泛素赖氨酸48位点

Treatment of An Acute Severe Cadmium Poisoning Patient Combined with Multiple Organ Dysfunction Syndromes by Integrated Chinese and Western Medicines:A Case Report

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Chinese Journal of Integrative Medicine 2020年第11期26卷 853-856页

作者： zhou Yuan-shen ZENG Rui-xiang ZHANG min-zhou GUO li-heng The Second Clinical College of Guangzhou University of Chinese Medicine Guangzhou(510120)China Department of Critical Care Medicine Guangdong Provincial Hospital of Chinese MedicineGuangzhou(510120)China

Cadmium(Cd),a toxic heavy metal commonly found in the environment,can cause toxic reactions at a dose of 30 *** is absorbed into the body mainly through the respiratory tract,digestive tract,and skin.(1)About 10%–50%of Cd absorption is inhaled dust,then about 5%–10%of ingested Cd is absorbed by body,which depending on particle size.(2)As known,most of Cd poisoning reaction is chronic,and acute Cd poisoning,especially high doses of acute poisoning,is very rare in *** to its airless and tasteless characteristics.

关键词： doses acute respiratory

MARS模型在渭河流域参考作物蒸散量计算中的适应性研究

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节水灌溉 2024年第2期 17-24页

作者：葛杰周晓平王晶曹绮欣曹钧恒陈至立冯家豪中国电建集团西北勘测设计研究院有限公司西安710065 咸阳市水利水电规划勘测设计研究院陕西咸阳712000

为有效提高气象资料缺失时渭河流域参考作物蒸散量(ET_(0))计算精度,选取流域及附近20个气象站58 a(1960-2017年)逐日气象资料,基于不同气象要素组合,构建16种基于多元自适应回归样条(MARS)的ET_(0)计算模型,并将计算结果与Hargreaves-S... 详细信息

为有效提高气象资料缺失时渭河流域参考作物蒸散量(ET_(0))计算精度,选取流域及附近20个气象站58 a(1960-2017年)逐日气象资料,基于不同气象要素组合,构建16种基于多元自适应回归样条(MARS)的ET_(0)计算模型,并将计算结果与Hargreaves-Samani、Makkink和Irmark-Allen模型进行对比,评价MARS模型在渭河流域的适应性及可移植性。结果表明:MARS模型能很好地甄别ET_(0)与各输入因子间的非线性关系,MARS2(T_(max)、T_(min)、R_(a))计算精度(平均MAE为0.225 mm/d,平均RMSE为0.327 mm/d,平均R2为0.897)能满足应用要求,模型精度随输入气象要素数量的增加而升高;输入因子中引入地球外辐射R_(a),可明显提高MARS模型精度;在输入因子相同时,MARS模型精度高于Hargreaves-Samani、Irmark-Allen和Makkink模型;MARS模型在渭河流域具有很强的泛化能力和可移植性。因此,气象资料缺失时基于MARS建立的ET_(0)计算模型可作为渭河流域ET_(0)计算的推荐模型。

关键词：渭河流域参考作物蒸散量多元自适应回归样条可移植性 ET_(0)模型地球外辐射R_(a)

Association of Comorbid Asthma and the Efficacy of Bioabsorbable Steroid-eluting Sinus Stents Implanted After Endoscopic Sinus Surgery in Patients with Chronic Rhinosinusitis with Nasal Polyps

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Current Medical Science 2023年第5期43卷 1005-1012页

作者： Ao HUANG Tao li min-shan li Zhen-xiao HUANG De-hui wang Lei Cheng Bing zhou heng wang Zheng liU Department of Otolaryngology-Head and Neck Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Institute of Allergy and Clinical Immunology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Hubei Clinical Research Center for Nasal Inflammatory Diseases Wuhan 430030China Department of Otolaryngology-Head and Neck Surgery Beijing Tongren HospitalCapital Medical UniversityBeijing 100000China Department of Otolaryngology-Head and Neck Surgery EyeEarNose and Throat HospitalFudan UniversityShanghai 200000China Department of Otorhinolaryngology&Clinical Allergy Center The First Affiliated HospitalNanjing Medical UniversityNanjing 210029China

Objective To identify factors affecting the efficacy of steroid-eluting sinus stents implanted after endoscopic sinus surgery(ESS)in patients with chronic rhinosinusitis with nasal polyps(CRSwNP).Methods We performed a post-hoc analysis of a randomized self-controlled clinical trial on post-operative implantation of bioabsorbable steroid-eluting stents in patients with *** logistic regression analysis was conducted to identify which of the following factors affect the response to post-operative stent implantation:sex,serum eosinophil levels,history of prior surgery,endoscopic scores,and comorbid conditions(asthma and allergic rhinitis).The primary outcome was the rate of post-operative intervention on day 30,and the secondary outcome was the rate of polypoid tissue formation(grades 2–3)on days 14,30,and *** A total of 151 patients with CRSwNP were included in the post-hoc *** was identified as the only risk factor for a poor response to steroid-eluting sinus stents on post-operative day 30,with an odds ratio of 23.71(95%CI,2.81,200.16;P=0.004)for the need for post-operative intervention and 19(95%CI,2.20,164.16;P=0.003)for moderate-to-severe polypoid tissue *** addition,the asthmatic group showed higher rates of post-operative intervention and polypoid tissue formation than the non-asthmatic group on post-operative day *** eosinophil levels were not identified as a risk factor for poor outcomes after stent *** Comorbid asthma,but not blood eosinophil level,impairs the efficacy of steroid-eluting sinus stents in the short term after ESS in patients with CRSwNP.

关键词： chronic rhinosinusitis asthma nasal polyps outcome steroid-eluting sinus stent

基于生物学特性及分子标记的贵州蜜环菌遗传多样性分析

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微生物学杂志 2024年第3期44卷 49-59页

作者：王彩云侯俊王永成忠均柳敏周茂嫦李恒谦张翔宇阮培均毕节市中药研究所贵州毕节551700 大方县乡村振兴局贵州毕节551700

为研究贵州天麻主产区蜜环菌的生物种及其遗传多样性,在贵州天麻主产区采集蜜环菌样本,利用双抗培养基分离菌索或子实体、菌材,定期观察并统计蜜环菌母种及原种的生物学特性;挑取菌索提取总DNA,利用真菌通用引物ITS和延伸因子EF1-α基... 详细信息

为研究贵州天麻主产区蜜环菌的生物种及其遗传多样性,在贵州天麻主产区采集蜜环菌样本,利用双抗培养基分离菌索或子实体、菌材,定期观察并统计蜜环菌母种及原种的生物学特性;挑取菌索提取总DNA,利用真菌通用引物ITS和延伸因子EF1-α基因扩增rDNA-ITS、rDNA-EF1-α片段并送测序及鉴定;用DNAStar软件进行GC含量分析及多序列比对,用MEGA软件构建进化树。从贵州毕节各县区、黔东南州雷山县等分离到17株蜜环菌,并与生产上普遍用的A9、京234做比较,通过生物学特性初步分析各蜜环菌的形态差异;通过rDNA-ITS、rDNA-EF1-α扩增,得到19株菌株的rDNA-ITS、rDNA-EF1-α完整序列,经GC含量分析、多序列比对、BLAST比对鉴定以及系统发育分析,将19株菌株分为3个种。通过对蜜环菌菌株生物学特性及rDNA-ITS、rDNA-EF1-α序列分析,发现贵州天麻主产区蜜环菌野生资源主要为Armillaria sp.,其次为Armillaria mellea、Armillaria gallica。

关键词：蜜环菌天麻生物学特性 rDNA-ITS 延伸因子