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Efficient genome editing in rice with miniature Cas12f variants

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aBIOTECH 2024年第2期5卷 184-188页

作者： Zhengyan Ye Yuanyan Zhang Shiqi He Shaokang Li Longjiong Luo yanbiao zhou Junjie Tan Jianmin Wan Sanya Institute of Nanjing Agricultural University State Key Laboratory of Crop Genetics&Germplasm Enhancement and UtilizationProvince and Ministry Co-sponsored Collaborative Innovation Center for Modern Crop ProductionNanjing Agricultural UniversityNanjing 210095China Zhongshan Biological Breeding Laboratory No.50 Zhongling StreetNanjing 210014China Key Laboratory of Southern Rice Innovation&Improvement Ministry of Agriculture and Rural Affairs/Hunan Engineering Laboratory of Disease and Pest Resistant Rice BreedingYuan Longping High-Tech Agriculture Co.LtdChangsha 410001China

Genome editing,particularly using the CRISPR/Cas system,has revolutionized biological research and crop *** the widespread use of CRISPR/Cas9,it faces limitations such as PAM sequence requirements and challenges in delivering its large protein into plant *** hypercompact Cas12f,derived from Acidibacillus sulfuroxidans(AsCas12f),stands out due to its small size of only 422 amino acids and its preference for a T-rich motif,presenting advantageous features over ***,its editing efficiency is extremely low in *** studies have generated two AsCas12f variants,AsCas12f-YHAM and AsCas12f-HKRA,demonstrating higher editing efficiencies in mammalian cells,yet their performance in plants remains *** this study,through a systematic investigation of genome cleavage activity in rice,we unveiled a substantial enhancement in editing efficiency for both AsCas12f variants,particularly for AsCas12f-HKRA,which achieved an editing efficiency of up to 53%.Furthermore,our analysis revealed that AsCas12f predominantly induces deletion in the target DNA,displaying a unique deletion pattern primarily concentrated at positions 12,13,23,and 24,resulting in deletion size mainly of 10 and 11 bp,suggesting significant potential for targeted DNA deletion using *** findings expand the toolbox for efficient genome editing in plants,offering promising prospects for precise genetic modifications in agriculture.

关键词： CRISPR/Cas Cas12 AsCas12f Rice Genome editing

Microstructure and Properties of SemisolidA356 Alloy Strip Affected by Nozzle Temperature of a Novel Micro Fused-Casting

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Journal of Wuhan University of Technology(Materials Science) 2020年第3期35卷 653-657页

作者： LUO Xiaoqiang LI Qingbin XUE Lingwei LI Yanling HAN Yongjun zhou yanbiao Henan Key Laboratory of Research for Central Plains Ancient Ceramics Pingdingshan UniversityPingdingshan 467000China .Insti-tute of Mechanics Chinese Academy of SciencesBeijing 100190China

The semisolid A356 alloy strip was prepared by a novel continuous micro fused-casting process. The microstructure evolution and mechanical property of A356 aluminum alloy strip with different nozzle temperatures were investigated. The nozzle temperature had great influences on the microstructure and property primarily accompanied with the crystal change in the fused-casting area through the cooling conditions. The results showed that the semisolid A356 alloy strip samples fabricated by micro fused-casting demonstrated good performances and uniform structures with the nozzle temperature at 593 ℃ and the stirring velocity at 700 r/min. The fine grains of the primary α-Al phase with average grain size of 51 μm and shape factor up to 0.71 were obtained under the micro fused-casting process, and the ultimate average vickers hardness came up to 83.39±0.89 HV, and the tensile strength and elongation of the A356 alloy strip reached 245.32 MPa and 7.85%, respectively.

关键词： semisolid A356 aluminum alloy nozzle temperature micro fused-casting

Effect of Pouring Temperature on Microstructure and Properties of A356 Alloy Strip by a Novel Semisolid Micro Fused-Casting for Metal

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Journal of Wuhan University of Technology(Materials Science) 2019年第5期34卷 1205-1209页

作者： LUO Xiaoqiang HAN Yongjun Henan Key Laboratory of Research for Central Plains Ancient Ceramics Pingdingshan UniversityPingdingshan 467000China Institute of Mechanics Chinese Academy of SciencesBeijing 100190China

A novel semisolid micro fused-casting(MFC) for preparing A356 alloy strips is proposed, and the effects of process parameters of pouring temperature on the microstructure and properties of A356 alloy strips are investigated. MFC means that the semisolid metal slurry was pressed out from the outlet of bottom of crucible to the movable plate, and directly solidified and formed layer by layer. The microstructure and properties of A356 semisolid alloy slurry were influenced by the cooling conditions. Results show that the aluminu alloy A356 strip samples fabricated by micro fused-casting had good performances and uniform structures with the pouring temperature at 595 ℃ and the substrate movement speed at 18 mm/s. The fine grains of the primary a-Al phase with average grain size of 53 μm and shape factor up to 0.72 was obtained, the ultimate tensile strength of the aluminum alloy A356 strip reaches 243.79±3.91 MPa, while the average vickers hardness is 82.65±1.86 HV.

关键词： A356 alloy semisolid micro fused-casting pouring temperature

Over-expressing a fungal NADP(H)-dependent glutamate dehydrogenase PcGDH improves nitrogen assimilation and growth quality in rice

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Over-expressing a fungal NADP(H)-dependent glutamate dehydro...

以Alcatel公司GSM系统为基础探讨地区的移动通信网络优化

2013全国植物生物学大会

作者： zhou yanbiao LIN Jianzhong LIU Hong zhou Xiangcheng YANG Yuanzhu DU Changqing LI Yixing LIU Derong TANG Dongying ZHAO Xiaoying ZHU Yonghua LIU Xuanming Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation College of BiologyHunan University College of Resources and Environment Fujian Agriculture and Forestry University Academy of Seed Industry of Hunan Yahua

In higher plants,glutamate dehydrogenase(GDH) has a lower affinity for ammonium than glutamine synthetase(GS),so nitrogen is mainly assimilated as ammonium by the GS/glutamate synthase(GOGAT) pathway

关键词： rice Pleurotus cystidiosus NADP (H)-dependent glutamate dehydrogenase ammonium assimilation

来源： cnki会议 cnki会议评论

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辽宁省交通高等专科学校学报 2007年第1期9卷 51-52页

作者：周彦彪中国移动通信集团辽宁有限公司网管中心辽宁沈阳110179

移动通信网络优化是一项长期的工作,本文通过对Alcatel公司的GSM系统的分析,找出了影响网络运行质量的原因,并针对各类原因提出了解决问题的若干措施。

关键词：移动通信网络优化掉话率拥塞率通话干扰

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水稻镉积累影响因素与低镉稻米生产策略

中国水稻科学 2021年第4期35卷 342-351页

作者：江南颜旭周延彪周群丰王凯杨远柱袁隆平农业高科技股份有限公司农业农村部南方水稻品种创制重点实验室/抗病虫水稻育种湖南省工程实验室长沙410125 华中农业大学植物科学技术学院武汉430070 湖南亚华种业科学研究院长沙410604 湖南农业大学农学院长沙410128

镉是一种生物毒性极强且分布广泛的重金属元素。农田中的镉不仅影响作物的生长发育,而且可通过食物链进入人体内,当富集到一定程度会危害人体健康。水稻是我国重要的粮食作物之一,在保障我国粮食安全中的地位举足轻重,但水稻同时也是对... 详细信息

镉是一种生物毒性极强且分布广泛的重金属元素。农田中的镉不仅影响作物的生长发育,而且可通过食物链进入人体内,当富集到一定程度会危害人体健康。水稻是我国重要的粮食作物之一,在保障我国粮食安全中的地位举足轻重,但水稻同时也是对镉吸收和积累最强的大宗谷类作物之一。近些年“镉大米”事件在我国频繁发生,稻米镉污染已成为一个备受社会关注的严峻问题,治理稻米镉污染迫在眉睫。本文从低镉水稻品种筛选与培育、优化水分管理、调节土壤pH值、施用叶面阻隔剂等方面对稻米镉污染治理技术的研究进展进行了综述,旨在为技术的集成、推广和应用提供理论依据,同时为新技术的研发提供新的思路。

关键词：水稻镉积累食品安全品种灌溉 pH

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中国水稻商业化育种成就与展望

中国稻米 2021年第4期27卷 35-44页

作者：杨远柱王凯符辰建秦鹏胡小淳谢志梅刘珊珊周延彪江南袁隆平农业高科技股份有限公司/农业农村部南方水稻品种创制重点实验室/抗病虫水稻育种湖南省工程实验室长沙410128 湖南亚华种业科学研究院长沙410128 湖南隆平高科种业科学研究院有限公司湖南宁乡410604

种业是农业的“芯片”,是粮食安全的根基,是国家战略性、基础性核心产业。种业发展关系到国家根本,中央更是将“解决好种子和耕地问题”列为2021年我国经济八大重点任务之一,并立志打一场种业翻身仗。本文系统阐述了商业化育种的内涵,... 详细信息

种业是农业的“芯片”,是粮食安全的根基,是国家战略性、基础性核心产业。种业发展关系到国家根本,中央更是将“解决好种子和耕地问题”列为2021年我国经济八大重点任务之一,并立志打一场种业翻身仗。本文系统阐述了商业化育种的内涵,并总结了我国在水稻商业化育种方面取得的成绩,以及存在的问题,以期对我国水稻及其他农作物商业化育种体系完善和发展提供参考。

关键词：水稻商业化育种种业

Enhanced Photoelectrochemical Properties of Cu_2O-loaded Short TiO_2 Nanotube Array Electrode Prepared by Sonoelectrochemical Deposition

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Nano-Micro Letters 2010年第4期2卷 277-284页

作者： yanbiao Liu Haibin zhou Jinhua Li Hongchong Chen Di Li Baoxue zhou Weimin Cai School of Environmental Science and Engineering Shanghai Jiao Tong University

Copper and titanium remain relatively plentiful in earth ***,using them in solar energy conversion technologies are of significant *** this work,cuprous oxide（Cu2O）-modified short TiO2 nanotube array electrode was prepared based on the following two design ideas:first,the short titania nanotubes obtained from sonoelectrochemical anodization possess excellent charge separation and transportation properties as well as desirable mechanical stability;second,the sonoelectrochemical deposition technique favours the improvement in the combination between Cu2O and TiO2 nanotubes,and favours the dispersion of Cu2O ***-Vis absorption and photo-electronchemical measurements proved that the Cu2O coating extended the visible spectrum absorption and the solar spectrum-induced photocurrent *** AM1.5 irradiation,the photocurrent density of the composite electrode（*** deposition for 5 min） was more than 4.75 times as high as the pure nanotube *** the photoactivity of the Cu2O/TiO2 electrode obtained using sonoelectrochemical deposition with others that synthesized using plain electrochemical deposition,the photocurrent density of the former electrode was 2.2 times higher than that of the latter when biased at 1.0 V（***/AgCl）.The reproducible photocurrent response under intermittent illumination demonstrated the excellent stability of the composite *** kind of composite electrode material will have many potential applications in solar cell and other fields.

关键词： Cu2O Short TiO2 nanotube array Sonoelectrochemical deposition

基于OBE理念的物理化学实验教学改革与实践——以平顶山学院为例

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大学化学 2023年第1期38卷 34-40页

作者：贺国旭吴华涛张秋霞周延彪刘丹丹平顶山学院化学与环境工程学院河南平顶山467000

针对当前物理化学实验教学中存在的问题,从课程目标的制定、应用型课程建设、课程资源建设、评价体系的重构等方面提出以学生为中心的OBE(Outcomes-based education)理念教学实施方案,结合实际案例,将OBE教学理念融入到课前导学、课中... 详细信息

针对当前物理化学实验教学中存在的问题,从课程目标的制定、应用型课程建设、课程资源建设、评价体系的重构等方面提出以学生为中心的OBE(Outcomes-based education)理念教学实施方案,结合实际案例,将OBE教学理念融入到课前导学、课中实操和课后强化训练中,发挥了学生的主观能动性,学生的综合创新能力得到提升,教学效果明显改善。

关键词：物理化学实验 OBE理念教学改革

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