检索结果-南通市图书馆

一株拮抗菌产生的抗菌物质的分离纯化与初步鉴定

在线全文

学校读者我要写书评

暂无评论

饲料工业 2024年第8期45卷 104-109页

作者：夏尚远薛东红周启升孙凤霞夏尚飞刘训理枣庄市农业综合执法支队山东枣庄277100 山东农业大学山东泰安271000 山东交通职业学院山东泰安271000 新泰市畜牧兽医事业发展服务中心山东泰安271000 枣庄职业学院山东枣庄277100

试验旨在分离纯化XDH菌株产生的抗菌物质,并初步鉴定有效抗菌物质的结构。从泰山林地土壤中分离获得一株对大肠杆菌及鸡白痢沙门氏菌等多种动物致病菌具有较强拮抗作用的短短芽孢杆菌XDH,通过硫酸铵盐析和层析技术对该菌株产生的抗菌物... 详细信息

试验旨在分离纯化XDH菌株产生的抗菌物质,并初步鉴定有效抗菌物质的结构。从泰山林地土壤中分离获得一株对大肠杆菌及鸡白痢沙门氏菌等多种动物致病菌具有较强拮抗作用的短短芽孢杆菌XDH,通过硫酸铵盐析和层析技术对该菌株产生的抗菌物质进行了分离纯化,对纯化后的抗菌物质进行了性质研究,并对抗菌物质B组分进行了结构鉴定。结果为:经过CM Sepharose FF离子交换层析、Sephadex G25凝胶层析对抗菌物质进行分离纯化,纯化后的抗菌物质对大肠杆菌的最小抑菌浓度(MIC)、最小杀菌浓度(MBC)分别为2.34μg/mL和4.69μg/mL,对鸡白痢沙门氏菌的MIC、MBC分别为0.63μg/mL和1.25μg/mL。采用AKTA Explore10层析系统对抗菌物质进一步分离纯化,获得A、B、C三个活性组分,其中,活性组分B的质谱解析结果显示,其分子量为1570.9 Da,推断为一新的多肽类抗菌物质。

关键词：大肠杆菌拮抗菌抗菌物质分离纯化结构鉴定

同方期刊数据库

Gypenosides ameliorate morphine-induced immunosuppression with an increased proportion of thymic T lymphocyte subsets and are involved in the regulation of the cAMP-CREM/CREB-IL-2 pathway

在线全文

学校读者我要写书评

暂无评论

Genes & Diseases 2024年第3期11卷 120-123页

作者： Hui Wang Zhonghao Li qisheng Wang Weixin Lin Ziting zhou Xinru Mu Yongwei Jiang Shengfeng Lu Shaodong Chen Zhigang Lu Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine in Prevention and Treatment of Tumor Nanjing University of Chinese MedicineNanjingJiangsu 210023China Key Laboratory of Acupuncture and Medicine Research of Ministry of Education Nanjing University of Chinese MedicineNanjingJiangsu 210023China College of Pharmacy Nanjing University of Chinese MedicineNanjingJiangsu 210023China School of Medicine Xiamen UniversityXiamenFujian 361102China

Opioid abuse can suppress the lymphatic system function,and produce severe immunosuppression that poses a significant risk of opportunistic infections such as methicillinresistant Staphylococcus aureus(MRSA)pneumonia.^(1,2)Gypenosides(Gps)are the most important immunomodulator components in the Chinese herbal medicine Gynostemma pentaphyllum.

关键词： herbal Gypenosides immunosuppression

On Designs of Decentralized Reputation Management for Permissioned Blockchain Networks

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Computer Modeling in Engineering & Sciences 2024年第5期139卷 1755-1773页

作者： Jinyu Chen Long Shi qisheng Huang Taotao Wang Daojing He School of Electronic and Optical Engineering Nanjing University of Science and TechnologyNanjing210094China School of Mechanical Engineering and Automation Harbin Institute of TechnologyShenzhen518055China College of Electronics and Information Engineering Shenzhen UniversityShenzhen518060China School of Computer Science and Technology Harbin Institute of TechnologyShenzhen518055China

In permissioned blockchain networks,the Proof of Authority(PoA)consensus,which uses the election of authorized nodes to validate transactions and blocks,has beenwidely advocated thanks to its high transaction throughput and fault ***,PoA suffers from the drawback of centralization dominated by a limited number of authorized nodes and the lack of anonymity due to the round-robin block proposal *** a result,traditional PoA is vulnerable to a single point of failure that compromises the security of the blockchain *** address these issues,we propose a novel decentralized reputation management mechanism for permissioned blockchain networks to enhance security,promote liveness,and mitigate centralization while retaining the same throughput as traditional *** paper aims to design an off-chain reputation evaluation and an on-chain reputation-aided ***,we evaluate the nodes’reputation in the context of the blockchain networks and make the reputation globally verifiable through smart ***,building upon traditional PoA,we propose a reputation-aided PoA(rPoA)consensus to enhance securitywithout sacrificing *** particular,rPoA can incentivize nodes to autonomously form committees based on reputation authority,which prevents block generation from being tracked through the randomness of reputation ***,we develop a reputation-aided fork-choice rule for rPoA to promote the network’s ***,experimental results show that the proposed rPoA achieves higher security performance while retaining transaction throughput compared to traditional PoA.

关键词： Blockchain reputation management PoA throughput security decentralization

Transcriptome-based analysis of key signaling pathways affecting the formation of primordial germ cell in chickens

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2024年第5期23卷 1644-1657页

作者： Ying Ding Qiong Zhi qisheng Zuo Kai Jin Wei Han Bichun Li Key Laboratory of Animal Genetics Breeding and Molecular Design of Jiangsu ProvinceCollege of Animal Science and TechnologyYangzhou UniversityYangzhou 225009China Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China Yangzhou UniversityYangzhou225009China Poultry Research Institute Chinese Academy of Agricultural Sciences/Jiangsu Institute of Poultry ScienceYangzhou 225009China

Bone morphogenetic protein 4(BMP4)can induce the formation of chicken primordial germ cells(PGCs)in vitro;however,its regulatory mechanism in poultry remains *** study aimed to use RNA-seq to analyze PGCs in chicken embryos and iPGCs induced by BMP4 in vitro,clarify the internal regulatory factors of PGCs,analyze the mechanism of the formation of PGCs,and lay a theoretical foundation for the further optimization of PGCs induction *** stem cells(ESCs),PGCs and iPGCs induced by BMP4 in vitro were *** transcriptional maps of the three cell types were studied using *** results showed 6,142 genes differentially expressed between PGCs and iPGCs,of which 2,728 were upregulated in iPGCs and 3,414 were downregulated in *** to that in ESCs,BMP4 was significantly upregulated in PGCs and *** results showed that both the TGF-βand Wnt signaling pathways were activated during the formation of PGCs in vitro and in vivo,and the activation was more significant during iPGCs induced by *** expression of Nodal,an inhibitory factor of TGF-βsignaling,was significantly decreased in PGCs and iPGCs,but was not expressed in iPGCs,which further supports our ***,the Lysosome and PI3K-AKT signaling pathways were significantly enriched in PGCs and iPGCs,***,transmission electron microscopy(TEM)results showed that the number of autolysosomes was significantly higher after the addition of BMP4,which is consistent with the KEGG ***,the number of PGCs was significantly reduced after ATG14 was interfered in vivo and in *** conclusion,this study screened out the key signaling pathways during the formation of PGCs,aiming to provide help for enriching the mechanism network regulating PGCs formation in chicken and laying a theoretical foundation for further improving the efficiency of inducing PGCs in vitro.

关键词： RNA-seq BMP4 PGCs ATG14 chicken

Scheme Based on Multi-Level Patch Attention and Lesion Localization for Diabetic Retinopathy Grading

在线全文

学校读者我要写书评

暂无评论

Computer Modeling in Engineering & Sciences 2024年第7期140卷 409-430页

作者： Zhuoqun Xia Hangyu Hu Wenjing Li qisheng Jiang Lan Pu Yicong Shu Arun Kumar Sangaiah School of Computer and Communication Engineering Changsha University of Science and TechnologyChangshaChina Hunan Province People’s Hospital(The First Affiliated Hospital of Hunan Normal University) ChangshaChina The First Affiliated Hospital of Guangxi Medical University NanningChina International Graduate Institute of AI National Yunlin University of Science and TechnologyYunlinChina Department of Electrical and Computer Engineering Lebanese American UniversityBayrutLebanon

Early screening of diabetes retinopathy(DR)plays an important role in preventing irreversible *** research has failed to fully explore effective DR lesion information in fundus ***,traditional attention schemes have not considered the impact of lesion type differences on grading,resulting in unreasonable extraction of important lesion ***,this paper proposes a DR diagnosis scheme that integrates a multi-level patch attention generator(MPAG)and a lesion localization module(LLM).Firstly,MPAGis used to predict patches of different sizes and generate a weighted attention map based on the prediction score and the types of lesions contained in the patches,fully considering the impact of lesion type differences on grading,solving the problem that the attention maps of lesions cannot be further refined and then adapted to the final DR diagnosis ***,the LLM generates a global attention map based on ***,the weighted attention map and global attention map are weighted with the fundus map to fully explore effective DR lesion information and increase the attention of the classification network to lesion *** paper demonstrates the effectiveness of the proposed method through extensive experiments on the public DDR dataset,obtaining an accuracy of 0.8064.

关键词： DDR dataset diabetic retinopathy lesion localization multi-level patch attention mechanism

New Pattern:the No.1 A-share Domestic Beauty Brand Changed Hands

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

China Detergent & Cosmetics 2024年第1期9卷 7-9页

作者： Chen qisheng Pingguan Technology(Wuhan)Media Co. China

On October 30,2023,A-share domestic beauty brands have all submitted their latest report *** from this list of report cards,it is clear that the competition pattern of domestic beauty companies has changed:in the first 3 quarters of 2023,Proya successfully topped the A-share domestic beauty brands list with a revenue of 5.249 billion yuan,surpassing Shanghai *** the start of the“Double 11”battle,Proya also led the way as the No.1 in Tmall beauty category in the pre-sale *** can be predicted that Proya will no doubt become the first in A-share domestic beauty brands in terms of revenue in 2023.

关键词： revenue companies doubt

MAL2 DNA methylation serves as a biomarker for the diagnosis and prognosis of glioma

在线全文

学校读者我要写书评

暂无评论

Genes & Diseases 2024年第5期11卷 36-39页

作者： Hao Luo Xing Xiao Weiliang Hou Jing Cai Ming Chen qisheng Tang Yusheng Tong Zengxin Qi Kaicheng Li Liang Chen Department of Neurosurgery Huashan HospitalShanghai Medical CollegeState Key Laboratory of Medical NeurobiologyMOE Frontiers Center for Brain Science and Institutes of Brain ScienceFudan UniversityShanghai 200040China National Center for Neurological Disorders Shanghai 200040China Shanghai Key Laboratory of Brain Function and Restoration and Neural Regeneration Shanghai 200040China Tianqiao and Chrissy Chen Institute Clinical Translational Research Center Shanghai 200040China Shanghai QuietD Biotechnology Co. Ltd.Shanghai 201210China

Glioma is a common and malignant brain tumor,and molecular diagnostics for glioma have received increasing attention.1,2 Previous studies have suggested that the MAL2 gene may be involved in the transcytosis of various cancers.3 This study aimed to investigate the potential of MAL2 as a biomarker for *** candidate MAL2 CpG sites were validated by pyrosequencing and used to construct a diagnostic model for *** analysis was also conducted to determine the relationship between highly methylated MAL2-specific CpG sites and the prognosis of *** findings also showed that MAL2 was more highly methylated in glioma than in other *** constructed diagnostic model can distinguish glioma from other cancers with high sensitivity(93.3%)and specificity(86.5%).Additionally,a risk score model was built based on MAL2 methylation to assess the prognosis of glioma。

关键词： glioma diagnosis MAL

激光光热效应对双层流体热毛细对流主动调控机理研究

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

空间科学学报 2024年第2期44卷 335-345页

作者：段文豪周小明陈启生河海大学机电工程学院常州213022 中国科学院力学研究所北京100190

热毛细对流不稳定性的主动调控是微重力流体力学和传热传质学领域的前沿问题,为了实现双层流体热毛细对流不稳定性的有效控制,提出利用激光光热效应对其不稳定性进行主动调控,并模型化研究了激光光热效应参数(激光束位置、激光功率和光... 详细信息

热毛细对流不稳定性的主动调控是微重力流体力学和传热传质学领域的前沿问题,为了实现双层流体热毛细对流不稳定性的有效控制,提出利用激光光热效应对其不稳定性进行主动调控,并模型化研究了激光光热效应参数(激光束位置、激光功率和光斑尺寸)对双层热毛细对流不稳定性的调控作用机理.计算结果显示,激光功率和光斑尺寸的调整能够显著改变双层流体热毛细对流的对流涡结构,并减小温度波的振动幅度.在一定工况下,热源附近温度波动范围由0.7 K变为0.1 K,变化显著;随光斑尺寸增大,温度振荡幅度先减小后增大.激光位置的调整能够影响液层局部对流涡的位置,进而改变激光位置两侧对流强度,实现局部区域的振荡不稳定流动的控制,通过光热效应参数的合理选择,可以实现对双层流体热毛细对流的有效控制.

关键词：双层流体热毛细对流光热效应主动调控

《对外关系法》中的国际条约规则评述——兼论国际条约在我国的适用方式

在线全文

学校读者我要写书评

暂无评论

中国法律评论 2024年第1期 212-224页

作者：何其生北京大学法学院

《对外关系法》重述了《宪法》《缔结条约程序法》关于条约缔结的规定,创新规定了善意履约原则、宪法至上原则、采取适当措施适用条约原则和公共秩序原则。善意履约的规定能够体现负责任大国的形象与担当,有助于条约在国内的实施和适用... 详细信息

《对外关系法》重述了《宪法》《缔结条约程序法》关于条约缔结的规定,创新规定了善意履约原则、宪法至上原则、采取适当措施适用条约原则和公共秩序原则。善意履约的规定能够体现负责任大国的形象与担当,有助于条约在国内的实施和适用。然而,条约与国内法律之间的关系有待厘清,条约的适用则需要具体规则来实施。条约在我国有间接适用、直接适用和混合适用三种方式。从规避条约适用的风险角度,间接适用最为稳妥,但全部间接适用既不现实也不必要。直接适用虽包含四种模式,即条约直接适用模式、条约优先适用模式、条约与国内法选择适用模式、条约与国内法重叠适用模式,但核心依旧是条约直接适用模式。为化解条约直接适用风险,落实《对外关系法》关于“采取适当措施实施和适用条约和协定”的规定,中国应加强已签署公约的全流程管理,在低风险领域尤其民商事条约上规定条约直接适用模式;在高风险领域采取“一事一议”制度,同时建立案例库。

关键词：《对外关系法》条约善意履约条约优先适用模式