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高分辨血管成像与定量方法研究进展(特邀)

激光与光电子学进展 2024年第2期61卷 377-394页

作者：刘翊婕王春承孟佳钱书豪周凌熙陈灵美刘智毅浙江大学光电科学与工程学院浙江杭州310027

主要综述高分辨血管成像技术及其在生物医学领域中的应用,侧重评述适用于高分辨血管图像的定量表征方法。血管图像定量表征主要包括3个步骤:图像预处理、血管图像重建及定量特征获取、定量参数的统计学分析。同时,对每个步骤中所涉及的... 详细信息

主要综述高分辨血管成像技术及其在生物医学领域中的应用,侧重评述适用于高分辨血管图像的定量表征方法。血管图像定量表征主要包括3个步骤:图像预处理、血管图像重建及定量特征获取、定量参数的统计学分析。同时,对每个步骤中所涉及的算法流程、准确性评估及后续算法的优化方向进行详尽的阐述。此外,探讨多种血管和血流参数所反映的生物学信息在临床上的参考意义,并结合具体的疾病场景,介绍多参数分析模型在区分不同疾病发展阶段方面的能力。本文的阐述不仅体现了高分辨血管成像技术及定量表征方法的潜在价值,也展示了它们在推进生物医学基础研究和临床诊断等方面的光明前景。

关键词：医学和生物成像高分辨成像血管造影层析成像图像处理定量表征参数分析

衣壳蛋白潜在乙酰化位点在马铃薯Y病毒侵染过程中的作用

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植物病理学报 2024年第2期54卷 462-468页

作者：姬丽云魏新钰周令希王凤龙田延平李向东山东农业大学植物保护学院植物病理学系泰安271018 中国农业科学院烟草研究所青岛276101

0 引言.马铃薯Y病毒(potato virus Y,PVY)是马铃薯Y病毒属(Potyvirus)的重要成员,可侵染170多种植物,其中茄科、藜科和豆科植物受害最为严重。1931年SMITH首次在马铃薯中发现PVY[1]。通过乙酰化组学在衣壳蛋白(coat protein, CP)中发现... 详细信息

0 引言.马铃薯Y病毒(potato virus Y,PVY)是马铃薯Y病毒属(Potyvirus)的重要成员,可侵染170多种植物,其中茄科、藜科和豆科植物受害最为严重。1931年SMITH首次在马铃薯中发现PVY[1]。通过乙酰化组学在衣壳蛋白(coat protein, CP)中发现多个潜在乙酰化位点,但它们在PVY侵染中的作用不清楚。本研究通过突变解析了CP中潜在乙酰化位点在PVY侵染珊西烟的致病力变化.

关键词：马铃薯Y病毒侵染过程衣壳蛋白致病力 PVY 豆科植物乙酰化修饰

In-situ measurement of atmospheric CFC-11 at the Shangdianzi Global Atmosphere Watch(GAW) Regional Station

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Science China Earth Sciences 2011年第2期54卷 298-304页

作者： ZHANG Fang zhou lingxi YAO Bo ZHANG XiaoChun XU Lin ZHANG XiaoLing zhou HuaiGang DONG Fan zhou LiYan Key Laboratory for Atmospheric Chemistry Centre for Atmosphere Watch and Services (CA WAS) Chinese Academy of Meteorological Sciences (CAMS) China Meteorological Administration ( CMA ) Beijing 100081 China Graduate University of Chinese Academy of Sciences Beijing 100049 China Institute of Urban Meteorology (IUM) China Meteorological Administration (CMA) Beijing 100089 China Miyun County Meteorological Bureau Beijing 101500 China

An in-situ GC-ECD system was used to measure halocarbons at Shangdianzi （SDZ） GAW regional station. In this paper, we reported observational results of atmospheric CFC- 11 （CCI3F） mixing ratios from April 2007 to March 2008. The CFC- 11 time series showed large variability. Approximately 62% observed values were filtered as non-background data. The median, 10% and 90% percentiles of CFC-11 background mixing ratios were 245.4 ppt （10-12 mol/mol）, 244.6 ppt and 246.1 ppt, respectively; whereas those of non-background CFC- 11 mixing ratios were 254.7, 246.6 and 272.1 ppt, respectively. Significant differences in background and non-background CFC-11 mixing ratios were observed between summer and autumn, mainly because of the CFC-11 stored in loam being prone to atmospheric release in hot seasons. Comparison of tile SDZ data with the five AGAGE stations suggested agreement with mid-high latitude Northern Hemisphere stations MHD, THD and RPB. The SDZ data were higher than that of Southern Hemisphere stations CGO and SMO. Higher CFC-11 mixing ratios measured in different seasons were always associated with winds from the W-WSW-SW sector, indicating that the airflow coming from this wind sector has a positive contribution to CFC- 11 concentrations. The CFC-11 mixing ratios were higher in autumn and summer than in spring and winter, in which its mixing ratios were very close to the atmospheric background level. This was happened especially when airflow originated from the NNE-NE-ENE-E sector, indicating the air masses coming from these wind directions was relatively clean.

关键词： SDZ background CFC-11 seasonal variation winds

Atmospheric sulfur hexafluoride in-situ measurements at the Shangdianzi regional background station in China

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Journal of Environmental Sciences 2014年第12期26卷 2451-2458页

作者： Bo Yao lingxi zhou Lingjun Xia Gen Zhang Lifeng Guo Zhao Liu Shuangxi Fang Guangzhou Institute of Geochemistry Chinese Academy of Sciences Chinese Academy of Meteorological Sciences (CAMS) China Meteorological Administration (CMA) University of Chinese Academy of Sciences Nanjing University of Information Science and Technology Meteorological Research Institute of Heilongjiang Province

We present in-situ measurements of atmospheric sulfur hexafluoride（SF6） conducted by an automated gas chromatograph–electron capture detector system and a gas chromatography/mass spectrometry system at a regional background site, Shangdianzi,in China, from June 2009 to May 2011, using the System for Observation of Greenhouse gases in Europe and Asia and Advanced Global Atmospheric Gases Experiment（AGAGE）techniques. The mean background and polluted mixing ratios for SF6 during the study period were 7.22 × 10-12（mol/mol, hereinafter） and 8.66 × 10-12, respectively. The averaged SF6 background mixing ratios at Shangdianzi were consistent with those obtained at other AGAGE stations located at similar latitudes（Trinidad Head and Mace Head）, but larger than AGAGE stations in the Southern Hemisphere（Cape Grim and Cape Matatula）. SF6 background mixing ratios increased rapidly during our study period, with a positive growth rate at 0.30 × 10-12year-1. The peak to peak amplitude of the seasonal cycle for SF6 background conditions was 0.07 × 10-12, while the seasonal fluctuation of polluted conditions was 2.16 × 10-12. During the study period, peak values of SF6 mixing ratios occurred in autumn when local surface horizontal winds originated from W/WSW/SW/SWS/S sectors, while lower levels of SF6 mixing ratios appeared as winds originated from N/NNE/NE/ENE/E sectors.

关键词： Atmospheric sulfur hexafluoride (SF6) In-situ measurement Shangdianzi Seasonality Data analysis

Estimating emissions of HCFC-22 and CFC-11 in China by atmospheric observations and inverse modeling

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Science China Chemistry 2012年第10期55卷 2233-2241页

作者： AN XingQin HENNE Stephan YAO Bo VOLLMER Martin K. zhou lingxi LI Yan Chinese Academy of Meteorological Sciences China Meteorological AdministrationBeijing 100081China Empa Swiss Federal Laboratories for Materials Testing and ResearchAir Pollution and Environmental TechnologyDubendorf Switzerland

This paper presents the estimation of Chinese emissions of HCFC-22 and CFC-11 in 2009 by an inverse modeling method based on in-situ measurement data from the Shangdianzi Global Atmosphere Watch (GAW) Regional Station (SDZ) and atmospheric transport simulations. After inversion (a-posteriori) estimates of the Chinese emissions in 2009 increased by 6.6% for HCFC-22 from 91.7 (± 83.6) to 98.3 (± 47.4) kt/yr and by 22.5% for CFC-11 from 13 (±12.6) to 15.8 (±7.2) kt/yr compared to an a-priori emission. While the model simulation with a-priori emissions already captured the main features of the observed variability at the measurement site, the model performance (in terms of correlation and mean-square-error) improved using a-posteriori emissions. The inversion reduced the root-mean-square (RMS) error by 4% and 10% for HCFC-22 and CFC-11, respectively.

关键词： FLEXPART model inversion modeling method emissions HCFC-22 and CFC-11 China

Evaluation of the carbon isotopic effects of NDIR and CRDS analyzers on atmospheric CO2 measurements

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Science China Earth Sciences 2016年第6期59卷 1299-1307页

作者： XIA LingJun zhou lingxi Marcel V.van der SCHOOT Chris W.RELLA LIU LiXin ZHANG Gen WANG HongYang Chinese Academy of Meteorological Sciences China Meteorological AdministrationBeijing 100081China Nanjing University of Information Science and Technology Nanjing 210044China Center for Australian Weather and Climate Research CSIRO Ocean and Atmosphere FlagshipAspendale 3195Australia Picarro Inc. Santa Clara 95054USA

Non-dispersive infrared （NDIR） and cavity ring-down spectroscopy （CRDS） CO2 analyzers use 12CO2 isotopologue absorption lines and are insensitive to all or part of other CO2-related isotopologues. This may produce biases in CO2 mole fraction measurements of a sample if its carbon isotopic composition deviates from that of the standard gases being used. To evaluate and compare the effects of carbon isotopic composition on NDIR and CRDS CO2 analyzers, we prepared three test sample air cylinders with varying carbon isotopic abundances and calibrated them against five standard cylinders with ambient carbon isotopic composition using CRDS and NDIR systems. We found that the CO2 mole fractions of the sample cylinders measured by G1301 （CRDS） were in good agreement with those measured by LoFlo （NDIR）. The CO2 values measured by both instruments were higher than that of a CO2 isotope measured by G2201i （CRDS） analyzer for a test cylinder with depleted carbon isotopic composition δ^13C =-36.828%0, whereas no obvious difference was found for other two test cylinders with 3 δ^13C=-8.630‰ and δ^13C=-15.380‰, respectively. According to the theoretical and experimental results, we concluded that the total CO2 mole fractions of samples with depleted isotopic compositions can be corrected on the basis of their 12CO2 values calibrated by standard gases using LoFlo and G1301 if the fi13C and fi180 values are known. Keywords NDIR and CRDS analyzers, Carbon isotopic effects, CO2 measurements

关键词： NDIR and CRDS analyzers Carbon isotopic effects CO2 measurements

Lonicera caerulea polyphenols inhibit fat absorption by regulating Nrf2-ARE pathway mediated epithelial barrier dysfunction and special microbiota

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Food Science and Human Wellness 2023年第4期12卷 1309-1322页

作者： Yuehua Wang Ningxuan Gao Andrea Nieto-Veloza lingxi zhou Xiyun Sun Xu Si Jinlong Tian Yang Lin Xinyao Jiao Bin Li College of Food Science Shenyang Agricultural UniversityShenyang 110161China Key Laboratory of Healthy Food Nutrition and Innovative Manufacturing of Liaoning Province Shenyang 110161China Instituto de Ciencia y Tecnología de Alimentos Universidad Nacional de ColombiaBogotá111311Colombia Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China College of Food Science and Technology Zhejiang University of TechnologyHangzhou 310014China

Scope:High-fat diet(HFD)induces imbalance in the small intestine environment,where fat digestion and absorption mainly take *** study aimed to elucidate the mechanisms by which Lonicera caerulea polyphenols(LCP)might inhibit fat absorption,from the perspective of small intestine microbiota and epithelial barrier *** and results:Male Sprague-Dawley rats were given HFD with or without co-administration of LCP for 8 *** results showed that LCP supplementation significantly decreased the levels of serum triglycerides(TG),total cholesterol(TC),and low-density lipoprotein cholesterol(LDL-C),and increased the contents of fecal sterols,in HFD *** also inhibited the dysfunction of the small intestine epithelial barrier,via alleviating the oxidative stress activated by Nrf2-ARE pathway,and by modulating the expressions of pro-inflammatory factors such as tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),cyclooxygenase-2(COX-2),nuclear factor kappa-B p65(NF-κB p65)and inducible nitric oxide synthase(iNOS)in the small ***,LCP administration restored the balance in small intestine microbiota and increased the abundance of the specific bacteria,such as Lactobacillus,involved in fat ***:Our results demonstrated that LCP may be beneficial to inhibit fat *** mechanism seems to be associated with the protection of the epithelial barrier integrity and the modulation of specific bacteria in the small intestine.

关键词： Lonicera caerulea berries Polyphenols Gut microbiota Oxidative stress Intestinal epithelial barrier

Simulation of CO_2 variations at Chinese background atmospheric monitoring stations between 2000 and 2009: Applying a CarbonTracker model

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Chinese Science Bulletin 2013年第32期58卷 3986-3993页

作者： CHENG YanLi AN XingQin YUN FangHua zhou lingxi LIU LiXin FANG ShuangXi XU Lin Key Laboratory for Atmospheric Chemistry of China Meteorological Administration Research Institute of Atmospheric Composition of Chinese Academy of Meteorological Sciences School of Environmental Science and Engineering Nanjing University of Information Science & Technology

We carried out a downscaling treatment over China using the CarbonTracker numerical model,which was applied using double grid nesting technology(3°×2°over the whole globe,1°×1°over China),simulating and analyzing atmospheric CO2concentrations over 10 recent years(2000–2009).The simulation results agreed very well with observed data from four background atmospheric monitoring stations in China(The periods for which the simulation results and observed values be compared were January2000 to December 2009 for the WLG station and June 2006 to December 2009 for the SDZ,LFS,and LAN stations),giving correlation coefficients of>*** high-resolution simulation data correlated slightly better than the low resolution simulation data with the observed data for three of the regions’atmospheric background *** analysis of the annual,seasonal CO2concentration variations at the background stations showed that the CO2concentration increased each year over the study period,with an average annual increase of more than 5%,and annual increases of more than 7%at the Shangdianzi and Lin’an *** CO2variations were greater at the Longfengshan station than at the Shangdianzi or Lin’an ***,the CO2concentrations were higher at the Shangdianzi and Lin’an stations because they are greatly affected by human activities in the Jingjinji and Changjiang Delta economic *** distribution in CO2concentrations and fluxes were higher in eastern than in western China.

关键词：二氧化碳浓度模拟数据中国西部大气监测数值模型追踪监测站 CO2浓度

Effects of freeze–thaw cycles on soil N_2O concentration and flux in the permafrost regions of the Qinghai–Tibetan Plateau

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Research in Cold and Arid Regions 2018年第1期10卷 69-79页

作者： ShengYun Chen Qian Zhao WenJie Liu Zhao Zhang Shuo Li HongLin Li ZhongNan Nie lingxi zhou ShiChang Kang Qilian Shan Station of Glaciology and Ecologic Environment State Key Laboratory of Cryospheric ScienceNorthwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesLanzhouGansu 730000China Institute of Tropical Agriculture and Forestry Hainan UniversityHaikouHainan 570228China State Key Laboratory of Plateau Ecology and Agriculture Qinghai UniversityXiningQinghai 810016China Department of Economic Development JobsTransport and ResourcesPrivate Bag 105Hamilton VIC 3300Australia Chinese Academy of Meteorological Sciences Beijing 100081China University of Chinese Academy of Sciences Beijing 100049China

Nitrous oxide(N_2 O) is one of the most important greenhouse gases in the atmosphere; freeze–thaw cycles(FTCs) might strongly influence the emission of soil N_2 O on the Qinghai–Tibetan Plateau(QTP). However, there is a lack of in situ research on the characteristics of soil N_2 O concentration and flux in response to variations in soil properties caused by ***, we report the effect of FTC-induced changes in soil properties on the soil N_2 O concentration and flux in the permafrost region of the higher reaches of the Shule River Basin on the northeastern margin of the QTP. We measured chemical properties of the topsoil, activities of soil microorganisms, and air temperature(AT), as well as soil N_2 O concentration and flux, over an annual cycle from July 31, 2011, to July 30, 2012. The results showed that soil N_2 O concentration was significantly affected by soil temperature(ST), soil moisture(SM), soil salinity(SS), soil polyphenol oxidase(SPO), soil alkaline phosphatase(SAP), and soil culturable actinomycetes(SCA), ranked as SM>SS>ST>SPO>SAP>SCA, whereas ST significantly increased soil N_2 O flux, compared with SS. Overall, our study indicated that the soil N_2 O concentration and flux in permafrost zone FTCs were strongly affected by soil properties, especially soil moisture, soil salinity, and soil temperature.

关键词： freeze-thaw cycles soil environment N2O