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Acetylacetone effectively controlled the secondary metabolites of Microcystis aeruginosa under simulated sunlight irradiation

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Journal of Environmental Sciences 2024年第1期135卷 285-295页

作者： Xiaomeng Wang Yixin Luo Shujuan Zhang lixiang zhou Department of Environmental Engineering College of Resources and Environmental SciencesNanjing Agricultural UniversityNanjing 210095China The State Key Laboratory of Pollution Control and Resource Reuse School of the EnvironmentNanjing UniversityNanjing 210023China

Inactivation of cyanobacterial cells and simultaneous control of secondary metabolites is of significant necessity for the treatment of cyanobacteria-laden ***(AcAc)has been reported a specific algicide to inactivate Microcystis aeruginosa(***)and an effective light activator to degrade *** study systematically investigated the photodegradation ability of AcAc under xenon(Xe)irradiation on the secondary metabolites of ***,mainly algal organic matter(AOM),especially toxic microcystin-LR(MC-LR).Results showed that AcAc outperformed H_(2)O_(2)in destructing the protein-like substances,humic acid-like matters,aromatic proteins and fulvic-like substances of *** MC-LR(250μg/L),0.05 mmol/L AcAc attained the same degradation efficiency(87.0%)as 0.1mmol/L H_(2)O_(2).The degradation mechanism of Xe/AcAc might involve photo-induced energy/electron transfer and formation of carbon center *** conditions(pH>9.0)were detrimental to the photoactivity of AcAc,corresponding to the observed degradation rate constant(k_(1)value)of MC-LR drastically decreasing to 0.0013 min^(-1)as solution pH exceeded *** PO_(4)^(3-)and HCO_(3)^(-)ions had obvious inhibition effects,whereas NO_(3)^(-)slightly improved k_(1)value from 0.0277 min^(-1)to 0.0321 min^(-1).The presence of AOM did not significantly inhibit MC-LR degradation in Xe/AcAc *** addition,the biological toxicity of MC-LR was greatly reduced after *** results demonstrated that AcAc was an alternative algicidal agent to effectively inactivate algal cells and simultaneously control the secondary metabolites after cell ***,the concentration and irradiation conditions should be further optimized in practical application.

关键词： Microcystis aeruginosa Photodegradation Acetylacetone Secondary metabolites Microcystin

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Conditioning treatments mitigate the levels of fecal pollution indicators in soil during land-application of sewage sludge

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Science China Technological Sciences 2024年

作者： WANG YuHang LI Hua FANG WenHao WANG Ru WANG XinXin ZHENG GuanYu zhou lixiang Department of Environmental Engineering College of Resources and Environmental Sciences Nanjing Agricultural University College of Materials and Chemical Engineering Pingxiang University Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization

Sewage sludge is a major source of fecal pathogens in the environment, and its application to land can result in a significant release of these pathogens into the soil. While conditioning treatments are crucial for improving the dewatering process of sludge, their impact on the presence and behavior of fecal pathogens in soil remains unclear. This study aims to assess four extraction methods for recovering fecal pollution indicators from soil amended with unconditioned and conditioned sludge. The indicators include Escherichia coli(EC), human-specific HF183 Bacteroides(HF183), human adenovirus(HAdV), human BK polyomavirus(BKPyV), human JC polyomavirus(JCPyV), and cross-assembly phage(crAssphage). This study also examines how soil moisture content affects the decay of these fecal pollution indicators in soil amended with raw sludge and investigates the influence of conditioning treatments with cationic polyacrylamide(PAM), Fenton's reagents, Fe[III]/CaO on their persistence in soil. The results indicated that the direct extraction method was the most effective and unbiased for recovering fecal pollution indicators from sludge-amended soil compared with the three elution-concentration methods. All fecal pollution indicators, except HAdV, remained stable under dry soil conditions, while high soil moisture content(48.39%-53.14%) slowed the decay rates of these indicators. During the application of sludge to soil, effective control of fecal pollution indicators was observed, with JCPyVand HAdV becoming undetectable within a short period. The log reduction values of HF183, BKPyV, and crAssphage ranged from 0.1 to 2.8 logs after 30 d of cultivation. The application of conditioned sludge, especially Fenton's reagent-conditioned sludge, resulted in a reduction in human fecal contamination in the receiving soil. Therefore, implementing appropriate conditioning methods, such as using Fenton's reagent, can effectively mitigate the health risks associated with fecal pathogens.

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Antibacterial potency of housefly larvae extract from sewage sludge through bioconversion

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Journal of Environmental Sciences 2013年第9期25卷 1897-1905页

作者： Chaocheng Zheng lixiang zhou Department of Environmental Engineering College of Resources and Environmental Science Nanjing Agricultural University

Use of the fly to convert sewage sludge into nutrient-rich soil conditioner and amendment is an attractive approach for sludge bioconversion. During this process, fecal coliforms, an indicating pathogen, in sludge were reduced to 5.3 × 102 most probable number /g dry solid from initial 3.32 × 106 MPN/g dry solid. It was also found that the extract of larvae grown in sludge during bioconversion have an observable inhibitory effect against bacteria compared to larvae grown in wheat bran as measured by minimum bacterial concentration tests. In vitro antimicrobial assay tests over time also showed that the extract had strong inhibitory efficiencies of ca. 99% against Bacillus cereus, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Serratia marcescens, while the efficiency was 69% and 57% against Bacillus subtilis and Klebsiella pneumoniae, respectively. The observed pathogenic bacterial cell membrane damage was found to be responsible for the phenomenon mentioned above, with nuclear acids leaching out quickly and alkaline phosphatase increasing in the outer membrane, followed by an increase of β-galactosidase in the inner membrane. Clearly, housefly larvae extract from sewage sludge through bioconversion possesses antibacterial potency against pathogenic bacteria.

关键词： sewage sludge larvae extract anti-bacterial membrane permeability

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Fabricating Fe3O4-schwertmannite as a Z-scheme photocatalyst with excellent photocatalysis-Fenton reaction and recyclability

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Journal of Environmental Sciences 2020年第12期32卷 186-195页

作者： Ting Li Jianru Liang lixiang zhou College of Resources and Environmental Sciences Nanjing Agricultural UniversityNanjing 210095China

Here we reported an effective method to solve the rate-limiting steps, such as the reduction of Fe^(3+) to Fe^(2+) and an invalid decomposition of H2O2 in a conventional Fenton-like reaction. A magnetic heterogeneous photocatalyst, Fe3O4-schwertmannite(Fe3O4-sch) was successfully developed by adding Fe3O4 in the formation process of schwertmannite. Fe3O4-sch shows excellent electrons transfer ability and high utilization efficiency of H2O2(98.5%). The catalytic activity of Fe3O4-sch was studied through the degradation of phenol in the heterogeneous photo-Fenton process. Phenol degradation at a wide pH(3-9) was up to 98% within 6 min under visible light illumination with the Fe3O4-sch as heterogeneous Fenton catalyst, which was higher than that using pure schwertmannite or Fe3O4. The excellent photocatalytic performance of Fe3O4-sch is ascribed to the effective recycling between ≡Fe^(3+) and ≡Fe^(2+) by the photo-generated electron, and also profit from the formation of the "Z-Scheme" system. According to the relevant data, photocatalytic mechanism of Fe3O4-sch for degrading phenol was proposed. This study not only provides an efficient way of enhancing heterogeneous Fenton reaction, but also gives potential application for iron oxyhydroxysulfate mineral.

关键词： Fe3O4 Schwertmannite Phenol degradation Photocatalytic activity Photocatalytic mechanism

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Enhancing sewage sludge dewaterability by bioleaching approach with comparison to other physical and chemical conditioning methods

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Journal of Environmental Sciences 2012年第8期24卷 1403-1410页

作者： Fenwu Liu Jun zhou Dianzhan Wang lixiang zhou Department of Environmental Engineering College of Resources and Environmental ScienceNanjing Agricultural UniversityNanjing 210095China.

The sewage sludge conditioning process is critical to improve the sludge dewaterability prior to mechanical dewatering. Traditionally, sludge is conditioned by physical or chemical approaches, mostly with the addition of inorganic or organic chemicals. Here we report that bioleaching, an efficient and economical microbial method for the removal of sludge-borne heavy metals, also plays a significant role in enhancing sludge dewaterability. The effects of bioleaching and physical or chemical approaches on sludge dewaterability were compared. The conditioning result of bioleaching by Acidithiobacillus thiooxidans and Acidithiobacillusferrooxidans on sludge dewatering was investigated and compared with the effects of hydrothermal （121℃ for 2 hr）, microwave （1050 W for 50 sec）, ultrasonic （250 W for 2 min）, and chemical conditioning （24% ferric chloride and 68% calcium oxide; dry basis）. The results show that the specific resistance to filtration （SRF） or capillary suction time （CST） of sludge is decreased by 93.1% or 74.1%, respectively, after fresh sludge is conditioned by bioleaching, which is similar to chemical conditioning treatment with ferric chloride and calcium oxide but much more effective than other conditioning approaches including hydrothermal, microwave, and ultrasonic conditioning. Furthermore, after sludge dewatering, bioleached sludge filtrate contains the lowest concentrations of chroma （18 times）, COD （542 mg/L）, total N （TN, 300 mg/L）, NH4＋-N （208 mg/L）, and total P （TP, 2 mg/L） while the hydrothermal process resulted in the highest concentration of chroma （660 times）, COD （18,155 mg/L）, TN （472 mg/L）, NH4＋-N （381 mg/L）, and TP （191 mg/L） among these selected conditioning methods. Moreover, unlike chemical conditioning, sludge bioleaching does not result in a significant reduction of organic matter, TN, and TP in the resulting dewatered sludge cake. Therefore, considering sludge dewaterability and the chemical properties of sludge filtrate and result

关键词： sludge bioleaching conditioning filtrate sludge cake

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Biodegradation of aniline by Candida tropicalis AN1 isolated from aerobic granular sludge

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Journal of Environmental Sciences 2011年第12期23卷 2063-2068页

作者： Dianzhan Wang Guanyu Zheng Shimei Wang Dewei Zhang lixiang zhou Department of Environmental Engineering College of Resources and Environmental Sciences Nanjing Agricultural University Nanjing 210095 China Department of Biology Hong Kong Baptist University Hong Kong 999077 China

Aniline-degrading microbes were cultivated and acclimated with the initial activated sludge collected from a chemical wastewater treatment plant. During the acclimation processes, aerobic granular sludge being able to effectively degrade aniline was successfully formed, from which a preponderant bacterial strain was isolated and named as AN1. Effects of factors including pH, temperature, and second carbon/nitrogen source on the biodegradation of aniline were investigated. Results showed that the optimal conditions for the biodegradation of aniline by the strain AN1 were at pH 7.0 and 28–35°C. At the optimal pH and temperature, the biodegradation rate of aniline could reach as high as 17.8 mg/（L·hr） when the initial aniline concentration was 400 mg/L. Further studies revealed that the addition of 1 g/L glucose or ammonium chloride as a second carbon or nitrogen source could slightly enhance the biodegradation efficiency from 93.0% to 95.1%–98.5%. However, even more addition of glucose or ammonium could not further enhance the biodegradation process but delayed the biodegradation of aniline by the strain AN1. Based on morphological and physiological characteristics as well as the phylogenetic analysis of 26S rDNA sequences, the strain AN1 was identified as Candida tropicalis.

关键词： aniline aerobic granular sludge biodegradation Candida tropicalis AN1

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HPLC指纹图谱结合化学模式识别研究不同产地桑枝质量

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国际中医中药杂志 2024年第6期46卷 750-756页

作者：周彦如刘雅琪谢作桦周立翔盛文胜江西中医药大学药学院南昌330004 江西德上医药研究院有限公司樟树331200

目的建立20批不同产地桑枝HPLC指纹图谱及多成分含量测定方法,结合相似度评价、聚类分析、主成分分析、偏最小二乘法判别分析(PLS-DA)等化学模式识别方法对不同产地桑枝进行质量评价。方法采用Wondasil C18色谱柱(4.6 mm×250 mm,5μm)... 详细信息

目的建立20批不同产地桑枝HPLC指纹图谱及多成分含量测定方法,结合相似度评价、聚类分析、主成分分析、偏最小二乘法判别分析(PLS-DA)等化学模式识别方法对不同产地桑枝进行质量评价。方法采用Wondasil C18色谱柱(4.6 mm×250 mm,5μm),检测波长254 nm,以乙腈(A)-0.2%冰醋酸(B)为流动相,梯度洗脱,流速0.8 ml/min;通过中药色谱指纹图谱相似度评价系统建立指纹图谱,计算相似度,采用SPSS 26.0及SIMCA 14.1软件进行化学模式识别。结果指纹图谱相似度计算结果显示,福建、浙江、河北产地相似度>0.830,其余产地相似度均>0.900;共标定14个共有峰,指认桑皮苷A、绿原酸、白藜芦醇苷、氧化白藜芦醇、白藜芦醇、桑色素、桑酮G、桑辛素8个成分;聚类分析、主成分分析将不同产地桑枝划分为两类,提取出4个主成分,累积方差贡献率89.247%,PLS-DA分析以VIP值大于1为标准,筛选出桑皮苷A、桑酮G、桑辛素3个质量差异标准物。结论建立的HPLC指纹图谱及含量测定方法操作简便,重复性、稳定性好,结果准确可靠,结合化学计量分析方法可对不同产地桑枝药材进行较完整的质量评价,为桑枝质量控制提供参考依据。

关键词：桑枝含量测定指纹图谱化学模式识别质量评价

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Box-Behnken响应面法优选樟帮酒润麸炒桑枝最佳炮制工艺

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国际中医中药杂志 2024年第8期46卷 1032-1038页

作者：刘雅琪周彦如谢作桦周立翔盛文胜江西德上医药研究院有限公司樟树331200 江西中医药大学药学院南昌330004

目的优选樟帮酒润麸炒桑枝最佳炮制工艺。方法以桑皮苷A、绿原酸、白藜芦醇、白藜芦醇苷、氧化白藜芦醇、桑色素、桑酮G、桑辛素8个成分总含量为考查指标, 对樟帮酒润麸炒桑枝中麦麸用量、黄酒用量、炒制温度、浸润时间、炮制时间5个影... 详细信息

目的优选樟帮酒润麸炒桑枝最佳炮制工艺。方法以桑皮苷A、绿原酸、白藜芦醇、白藜芦醇苷、氧化白藜芦醇、桑色素、桑酮G、桑辛素8个成分总含量为考查指标, 对樟帮酒润麸炒桑枝中麦麸用量、黄酒用量、炒制温度、浸润时间、炮制时间5个影响因素进行单因素考察, 运用Box-Behnken响应面法进行试验设计, 优选酒润麸炒桑枝最佳炮制工艺。结果响应面法优选樟帮酒润麸炒桑枝最佳炮制工艺为浸润时间116 min, 麦麸用量19%, 黄酒用量10%, 炒制时间7 min, 炒制温度237 ℃, 该工艺条件下, 桑枝饮片中8个成分平均总含量为787.8 μg/g, RSD值为0.98%。结论优选的樟帮酒润麸炒桑枝最佳炮制工艺稳定可靠、重复性好, 可更好保障樟帮酒润麸炒桑枝质量。

关键词：桑枝 Box-Behnken响应面法樟帮酒润麸炒炮制(中药)

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基于深度学习的肺结核检测综述

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中国医学物理学杂志 2024年第7期41卷 918-924页

作者：谢浩杰鲁明丽张陈周理想滕诣迪王明明盐城工学院电气工程学院江苏盐城221051 常熟理工学院电气与自动化工程学院江苏常熟215500 苏州大学机电工程学院江苏苏州215031 常熟理工学院电子信息工程学院江苏常熟215500

基于医学影像的肺结核病灶自动检测技术成为医学图像处理领域的研究热点。本研究围绕深度学习在肺结核病灶检测方面的相关研究与应用展开综述,首先阐述用于肺结核检测的实验基准,涵盖肺部医学影像的相关公开数据库和肺结核检测与分类竞... 详细信息

基于医学影像的肺结核病灶自动检测技术成为医学图像处理领域的研究热点。本研究围绕深度学习在肺结核病灶检测方面的相关研究与应用展开综述,首先阐述用于肺结核检测的实验基准,涵盖肺部医学影像的相关公开数据库和肺结核检测与分类竞赛的相关研究进展,然后提出肺结核检测领域中深度学习方法与应用的发展趋势,最后分析深度学习在肺结核诊断中面临的挑战。本研究从技术特性、性能优势、应用前景等方面对这些技术的研究进展以及面临的挑战进行总结和展望。

关键词：肺结核医学影像自动检测深度学习综述

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专业学位研究生影像诊断学师生共建案例库的改革探索

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中国继续医学教育 2024年第13期16卷 21-24页

作者：王敏红周理想张峥嵘皖南医学院第一附属医院/弋矶山医院放射科安徽芜湖241001

影像诊断学专注于研究各系统疾病的X线成像(Xradiography)、计算机体层摄影(computed tomography,CT)、磁共振成像(magnetic resonance imaging,MRI)的图像特征,是放射医学专业的主干课程,如何在有限的时间内培养学生独立思考、解决临... 详细信息

影像诊断学专注于研究各系统疾病的X线成像(Xradiography)、计算机体层摄影(computed tomography,CT)、磁共振成像(magnetic resonance imaging,MRI)的图像特征,是放射医学专业的主干课程,如何在有限的时间内培养学生独立思考、解决临床实际工作的能力及科研创新能力,是研究生教学改革的重心和目的。案例教学法有助于提升学生解决实际问题的能力,提高学习兴趣,因此是目前教学改革的主要方案之一。但是,案例教学的案例从何而来?如何调动更多的学生积极参与?如何让学生更加灵活的应用其所学知识?为解决这些问题,皖南医学院医学影像学教研室尝试以学生为主体,开展教师-研究生共建案例库的教学改革探索。文章剖析了师生共建案例库的意义,培养了学生的临床能力,提升了教师的全面素质;重点介绍了案例库具体实施方法,包括案例素材的收集、筛选及案例的制作和案例库导入;最后总结了师生共建案例库的效果。此次探索可为其他课程案例库的开发提供一定参考。

关键词：案例库影像诊断学专业学位研究生教学改革医学教育

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