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Molecular characterization of GmNHX2,a Na^+/H^+ antiporter gene homolog from soybean,and its heterologous expression to improve salt tolerance in Arabidopsis

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Chinese Science Bulletin 2009年第19期54卷 3536-3545页

作者： zhou guoan guan rongxia li yinghui chang ruzhen qiu lijuan National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)/Key Lab of Germplasm & Biotechnology (MOA) Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing 100081 China

Na+/H+ antiporters have been well documented to enhance plant salt tolerance by regulating cellular ion homeostasis. Here, a putative Na+/H+ antiporter gene homolog GmNHX2 from soybean was cloned and predicted to encode a protein of 534 amino acids with 10 putative transmembrane domains. GmNHX2 was expressed in all soybean plant tissues but enriched in roots and its expression was induced by NaCl and polyethylene glycol (PEG) treatments. GmNHX2 exhibits greater sequence similarity with LeNHX2 and AtNHX6 than that of AtNHX1 and AtSOS1. Although phylogenetic analysis clustered GmNHX2 with organellar (tonoplast and vesicles) antiporters, the GmNHX2-EGFP (enhanced green fluorescent protein) fusion protein was possibly localized in the plasma membrane or organelle membrane of transgenic plant cells. Furthermore, transgenic Arabidopsis plants expressing GmNHX2 were more tolerant to high NaCl concentrations during germination and seedling stages when compared with wild-type plants. These results suggest that GmNHX2 is a membrane Na+/H+ antiporter and may function to regulate ion homeostasis under salt stress.

关键词：植物耐盐基因工程盐胁迫离子分布植物组织 GmNHX2

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Development and utilization of a new chemically-induced soybean library with a high mutation density

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Journal of Integrative Plant Biology 2017年第1期59卷 60-74页

作者： Zhongfeng li lingxue Jiang Yansong Ma Zhongyan Wei Huilong Hong Zhangxiong liu Jinhui Lei Ying liu rongxia guan Yong Guo Longguo Jin lijuan Zhang yinghui li Yulong Ren Wei He Ming liu Nang Myint Phyu Sin Htwe lin liu Bingfu Guo Jian Song Bing Tan Guifeng liu Maiquan li Xianli Zhang Bo liu Xuehui Shi Sining Han Sunan Hua Fulai zhou lili Yu Yanfei li Shuang Wang Jun Wang ruzhen chang lijuan qiu National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization Ministry of AgricultureInstitute of Crop ScienceChinese Academy of Agricultural Science Soybean Research Institute Heilongjiang Academy of Agricultural Sciences College of Agriculture Yangtze University

Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate（EMS）-induced soybean（Glycine max） population,consisting of 21,600 independent M_2 lines, was *** 1,000 M_（4（5））families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll（Chl） content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to Minn Gold, where a different single nucleotide polymorphism variation in the Mg-chelatase subunit gene（Chli1a） results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker-assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean.

关键词： soybean mutant yellow chemically golden phenotype genomics mutation Glycine chloroplast

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