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Rice science 2022年第2期29卷 155-165页

作者： CHEN Yibo WANG Zhidong WANG Chongrong LI Hong HUANG Daoqiang zhou degui ZHAO Lei PAN Yangyang GONG Rong zhou Shaochuan Guangdong Academy of Agricultural Sciences Rice Research Institute/Guangdong Key Laboratory of New Technology in Rice BreedingGuangzhou 510640China

We used a widely targeted metabolomics approach to examine the metabolic changes in three pedigreed indica cultivars(Meixiangzhan 2,Meisizhan and Qixinzhan),with different eating qualities,at 8,15 and 30 d after flowering(DAF)to explore the formation mechanism of rice eating and nutritional qualities at a global metabolic level.A total of 623 metabolites were identified,and results showed that metabolic variations among rice cultivars decreased with grain developmental stage,suggesting that sufficient carbohydrate and amino acid supply during grain development may contribute to excellent rice eating and nutritional quality *** 19:0 and 16:1 were beneficial for excellent eating quality formation during grain *** fragrance was attributed mainly to spermidine andγ-aminobutyric *** cultivars with excellent eating quality at 15-30 DAF had relatively higher flavone content,possibly because they had adequate carbohydrate and amino acid contents during grain *** results provided insight into the mechanisms for establishing rice eating and nutritional qualities during grain formation at a global metabolic level,and can be applied towards improving rice quality.

关键词： eating quality metabolomics nutritional quality high-quality rice grain development

Breeding Effects and Genetic Compositions of a Backbone Parent (Fengbazhan) of Modern indica Rice in China

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Rice science 2022年第5期29卷 397-401,I0001-I0011页

作者： ZHAO Lei zhou Shaochuan WANG Chongrong LI Hong HUANG Daoqiang WANG Zhidong zhou degui CHEN Yibo GONG Rong PAN Yangyang Rice Research Institute Guangdong Academy of Agricultural SciencesGuangdong Laboratory for Lingnan Modern AgricultureGuangdong key Laboratory of New Technology in Rice Breeding/Guangdong Rice Engineering LaboratoryGuangzhou 510640China

Fengbazhan(FBZ)and its derived varieties have been widely cultivated in China,accounting for 22%of China’s paddy ***,evaluating the *** of FBZ and elucidating its genetic compositions are effective strategies for interpreting the history of modern rice *** integrative bioinformatics analysis and population validation,we found that the expression of favorable genes on chromosome 6 was important for the breeding of FBZ-derived varieties and these favorable genes were gradually optimized during the breeding process,which was consistent with the conjecture of the‘Rice Core Germplasm Breeding Theory’.For several decades,tremendous efforts have been made by Chinese scientists in rice breeding to improve grain yield,nutritional quality and environmental performance,thereby achieving substantial progress in global food *** rice breeding technologies have been developed,including semi-dwarf breeding,utilization of heterosis,launching the‘Super Rice Project’,development of green super rice and molecular design breeding(Qian et al,2016;Bai et al,2018;Tang and Cheng,2018;Yu et al,2022).In southern China,rice can be divided into the early,middle and late rice,and the cultivation area in southern China accounts for approximately 80%of China’s rice cultivation ***,several distinct rice varieties are widely cultivated in southern China.

关键词： breeding paddy dwarf

Stay-Green Panicle Branches Improve Processing Quality of Elite Rice Cultivars

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Rice science 2023年第1期30卷 11-14页

作者： CHEN Yibo ZHAO Lei WANG Chongrong LI Hong HUANG Daoqiang WANG Zhidong zhou degui PAN Yangyang GONG Rong zhou Shaochuan Rice Research Institute Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of New Technology in Rice Breeding/Guangdong Laboratory for Lingnan Modern AgricultureGuangzhou 510640China

Rice panicle branches are key routes for transportation of photo synthetic products to *** senescence is an important trait that the breeders considered when breeding elite rice cultivars in South ***,little is known about the mechanisms underlying the contributions of stay-green panicle branches to rice yield and ***,we reported stay-green branches that continuously maintained transportation function and provided carbohydrates for basal grain development at the late stages of rice *** ensured the coordination of development between apical and basal grains,and further improved the processing quality of elite *** findings provided strategies for improving the fullness and quality of rice grains and presented an important basis for cultivating elite rice.

关键词： elite breeding cultivar

OsLTP47 may function in a lipid transfer relay essential for pollen wall development in rice

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Journal of Genetics and Genomics 2022年第5期49卷 481-491页

作者： Libin Chen Chonghui Ji degui zhou Xin Gou Jianian Tang Yongjie Jiang Jingluan Han Yao-Guang Liu Letian Chen Yongyao Xie State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources College of Life SciencesSouth China Agricultural UniversityGuangzhouGuangdong 510642China Guangdong Laboratory for Lingnan Modern Agriculture GuangzhouGuangdong 510642China Guangdong Key Laboratory of New Technology in Rice Breeding Rice Research InstituteGuangdong Academy of Agricultural SciencesGuangzhouGuangdong 510640China Present address:Vector-borne Virus Research Center Fujian Province Key Laboratory of Plant VirologyFujian Agriculture and Forestry UniversityFuzhouFujian 350002China.

In plants,lipid transfer proteins(LTPs)transport pollen wall constituents from the tapetum to the exine,a process essential for pollen wall ***,the functional cooperation of different LTPs in pollen wall development is not well *** this study,we have identified and characterized a grassspecific LTP gene,Os LTP47,an important regulator of pollen wall formation in rice(Oryza sativa).Os LTP47 encodes a membrane-localized LTP and in vitro lipid-binding assays confirms that Os LTP47 has lipidbinding *** of Os LTP47 causes disordered lipid metabolism and defective pollen walls,leading to male *** two-hybrid and pull-down assays reveal that Os LTP47 physically interacts with another LTP,Os *** findings suggest that the plasma membrane-localized Os LTP47 may function as a mediator in a lipid transfer relay through association with cytosolic and/or locular Os C6 for pollen wall development and that various LTPs may function in a coordinated manner to transport lipid molecules during pollen wall development.

关键词： Rice Pollen wall Lipid transfer protein Male sterility

A 9.5-kb deletion in the 1st intron of OsMADS51 enhances temperature sensitivity in rice

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The Crop Journal 2024年第4期12卷 1031-1040页

作者： Lei Zhao Haifei Hu Junyu Chen Chongrong Wang Yibo Chen Hong Li Daoqiang Huang Zhidong Wang degui zhou Rong Gong Yangyang Pan Junliang Zhao Liangyong Ma Shaochuan zhou Rice Research Institute Guangdong Academy of Agricultural SciencesGuangzhou 510640GuangdongChina State Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement China National Rice Research InstituteHangzhou 310006ZhejiangChina Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510640GuangdongChina Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China(Co-construction by Ministry and Province) Ministry of Agriculture and Rural AffairsGuangzhou 510640GuangdongChina Guangdong Rice Engineering Laboratory Guangzhou 510640GuangdongChina Guangdong Key Laboratory of New Technology in Rice Breeding Guangzhou 510640GuangdongChina

Temperature is an important environmental factor affecting heading date of *** its importance,genes responsible for temperature-sensitive heading in rice have remained *** previous study identified a quantitative trait locus qHd1 which advances heading date under high temperatures.A 9.5-kb insertion was found in the first intron of OsMADS51 in indica variety Zhenshan 97(ZS97).However,the function of this natural variant in controlling temperature sensitivity has not been *** this study,we used CRISPR/Cas9 to knock out the 9.5-kb insertion in *** conducted under cotrolled conditions in phytotrons confirmed that deletion increased temperature sensitivity and advanced heading by downregulating the expression level of ***-hybrid assays in yeast,ChIP-quantitative polymerase chain reaction,electrophoretic mobility shift,and luciferase-based transient transactivation assays collectively confirmed that OsMADS51 affects heading date by regulation of heading date gene *** further determined that the long non-coding RNA HEATINR is generated from the first intron of OsMADS51,offering an explanation for how the 9.5-kb insertion affects temperature *** also found that OsMADS51 was strongly selected in early/late-season rice varieties in South China,possibly accounting for their strong temperature *** insights not only advance our understanding of the molecular mechanisms underlying the temperature-responsive regulation of heading date in rice but also provide a valuable genetic target for molecular breeding.

关键词： OsMADS51 Insertion Long non-coding RNA Oryza sativa

PhieCBEs:Plant High-Efficiency Cytidine Base Editors with Expanded Target Range

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Molecular Plant 2020年第12期13卷 1666-1669页

作者： Dongchang Zeng Taoli Liu Jiantao Tan Yaling Zhang Zhiye Zheng Bin Wang degui zhou Xianrong Xie Menghui Guo Yao-Guang Liu Qinlong Zhu State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangzhou 510642China Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510642China College of Life Sciences South China Agricultural UniversityGuangzhou 510642China 不详

Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)-mediated genome editing can efficiently produce gene-knockout *** the other hand,CRISPR/Cas-derived base editors offer the ability to induce precise nucleotide substitutions(Komor et al.,2016).Cytidine base editors(CBEs)consist of a cytidine deaminase fused with a Cas9-nickase variant(Cas9n,with a D10A substitu-tion)and can achieve site-specific C-to-T ***,adenine base editors use an adenine deaminase forA-to-G *** systems have been used in various organisms(Mishra et al.,2019).However,the Cas9 complex requires target sites containing NGG protospacer adjacent motifs(PAMs),thus restricting selection of potential targets.A number of CBEs have been developed using Cas9 variants(mostly Cas9n),cytidine deaminases(such as rAPOBEC1 and PmCDA1),and uracil glycosylase inhibitor(UGI)*** CBEs of the first generation(BE1,rAPOBEC1-dCas9),second generation(BE2,rAPOBEC1-dCas9-UGI),and third generation(BE3,rAPOBEC1-Cas9n-UGI)have moderate editing efficiencies in mammalians(Komor etal.,2016).

关键词： dCas9 CBE base

Copy number variation of NAL23 causes narrow-leaf development in rice

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Journal of Genetics and Genomics 2024年第8期51卷 880-883页

作者： degui zhou Yajing Li Xianrong Xie Wenyan Ding Libin Chen Tie Li Jianian Tang Xiyu Tan Weizhi Liu Yueqin Heng Yongyao Xie Letian Chen Qi Liu Shaochuan zhou Jing Zhao Gong Zhang Jiantao Tan Yaoguang Liu Rongxin Shen State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangdong Laboratory for Lingnan Modern AgricultureCollege of Life SciencesSouth China Agricultural UniversityGuangzhouGuangdong 510642China Guangdong Key Laboratory of New Technology in Rice Breeding Rice Research InstituteGuangdong Academy of Agricultural SciencesGuangzhouGuangdong 510640China Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes and MOE Key Laboratory of Tumor Molecular Biology Institute of Life and Health EngineeringJinan UniversityGuangzhouGuangdong 510632China

Leaf shape is a key trait for plant architecture relating to photosynthesis and transpiration in plants(Lawson et al.,2020).Erect leaves with proper leaf length and width,particularly the upper three leaves in cereal crops,could significantly improve light absorption efficiency in canopy and ultimately increase crop yield(Jiao et al.,2010).Rice leaves initiate at the peripheral zone of shoot apical meristem,then develop along the proximal-distal,adaxial-abaxial,and mediallateral axes to form a flat symmetric architecture(Du et al.,2018).

关键词： crops cereal narrow

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9个转基因杂交棉组合的优势比较研究

棉花科学 2015年第1期37卷 29-35页

作者：周德桂梅正鼎湖南省棉花科学研究所湖南常德415101

以转基因抗虫杂交棉9个（代号为B1-B9）新组合和湘杂棉7号（对照）为材料，进行生育性状、光合特性、产量性状和品质性状等方面进行系统的比较分析。研究结果表明：各组合与对照在生育期、株高、果枝数方面无显著差异；B3的早熟性、结... 详细信息

以转基因抗虫杂交棉9个（代号为B1-B9）新组合和湘杂棉7号（对照）为材料，进行生育性状、光合特性、产量性状和品质性状等方面进行系统的比较分析。研究结果表明：各组合与对照在生育期、株高、果枝数方面无显著差异；B3的早熟性、结铃性、叶绿素含量、籽棉产量和纤维品质均优于对照；B5、B6、B9的籽棉产量与对照无显著差异，B1、B2、B4、B7、B8叶绿素含量偏低、皮棉减产，品质也略差。综合各方面的因素，B3叶绿素含量高，有利于光合产物生产和供应，且铃大，结铃性强、产量高，纤维品质优，符合湖南省棉花新品种审定的产量及品质要求。

关键词：湖南杂种优势杂交棉光合特性产量品质

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长春铁路医院“出轨”剥离

中国卫生产业 2004年第7期1卷 25-27页

作者：孙扬周德贵

2004年1月1日,原长春铁路分局铁路医院从铁路系统分离,进入地方卫生系统序列。5月1日,长春铁路分局要求医院交回其曾批准使用的“长春铁路分局铁路医院”印章,医院只好给自己起了个命字叫“长春市铁路医院”,并“私刻”了相关印章。在这... 详细信息

2004年1月1日,原长春铁路分局铁路医院从铁路系统分离,进入地方卫生系统序列。5月1日,长春铁路分局要求医院交回其曾批准使用的“长春铁路分局铁路医院”印章,医院只好给自己起了个命字叫“长春市铁路医院”,并“私刻”了相关印章。在这5个多月的时间内,长春市卫生局只到铁路医院“考察”了一次干部,医院名称、医院编制、医保资格等问题久拖未决。铁路分局是肯定不管了,卫生局还没真来管——医院工作陷入停滞。

关键词：长春铁路医院改革发展管理人员定编

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发电机定子和转子事故烧损与对策

四川电力技术 1998年第1期21卷 8-13页

作者：周德贵四川电力试验研究院

四川电力工业局下属某厂，在冬季某夜晚，因特大浓雾引起电厂２２０ｋＶ输电线路污闪，形成单相弧光接地与相间短路，造成发电机不对称运行。因继电保护直流电源消失，使发电机不对称运行持续时间较长，导致２号发电机定子和２～４号发... 详细信息

四川电力工业局下属某厂，在冬季某夜晚，因特大浓雾引起电厂２２０ｋＶ输电线路污闪，形成单相弧光接地与相间短路，造成发电机不对称运行。因继电保护直流电源消失，使发电机不对称运行持续时间较长，导致２号发电机定子和２～４号发电机转子烧坏的特大型事故。文章对事故的原因和烧损的情况进行了仔细检查和总结。提出防止不对称运行的技术措施。

关键词：汽轮发电机烧毁发电机定子转子事故