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Ribotrap Analysis of Proteins Associated with FHL3 3'Untranslated Region in Glioma Cells

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Chinese Medical Sciences Journal 2014年第2期29卷 78-84页

作者： Wei Han Qing Xia Bin Yin Xiao-zhong peng State Key Laboratory of Medical Molecular Biology Department of Biochemistry and Molecular Biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100005 China State Key Laboratory of Proteomics Institute of Basic Medical Sciences National Center of Biomedical Analysis Beijing 100850 China

Objective To screen the proteins associated with four-and-a-half LIM domains 3(FHL3) 3' untranslated region(3'UTR) in glioma cells. Methods Western blot was adopted to detect the regulatory effect of poly(C)-binding protein 2(PCBP2) on FHL3. Biotin pull-down and sliver staining were employed to screen and verify the candidate binding proteins of FHL3 3'UTR. Then liquid chromatography-tandem mass spectrometry(LC-MS/MS) and molecule annotation system were used to identify and analyze the candidate binding proteins. Immunoprecipitation was conducted to study the interaction between PCBP2 and polypyrimidine tract-binding protein 1(PTBP1), a binding protein identified by LC-MS/MS. Results PCBP2 could bind to FHL3 mRNA 3'UTR-A and inhibited the expression of FHL3 in T98 G glioms cells. 22 candidate binding proteins were identified. Among them, there were 11 RNA binding proteins, including PCBP2. PTBP1 associated with FHL3 mRNA 3'UTR and interacted with PCBP2 protein. Conclusion PCBP2 and PTBP1 can both associate with FHL3 mRNA 3'UTR through forming a protein complex.

关键词： FHL3 3'untranslated region poly(C)-binding protein 2 polypyrimidine tract-binding protein 1 liquid chromatography-tandem mass spectrometry

Sichuan hot-spring snakes imperiled:reason,situation,and protection

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Zoological Research 2022年第1期43卷 95-97页

作者： Jin-Long Ren Chaochao Yan zhong-Liang peng Jia-Tang Li Chengdu Institute of Biology Chinese Academy of SciencesChengduSichuan 610040China University of Chinese Academy of Sciences Beijing 100049China Mangkang Ecological Station Xizang Ecological Safety Monitor NetworkChangduXizang 854500China

The Sichuan hot-spring snake(Thermophis zhaoermii)is a rare and endemic species found at high elevations in the Hengduan Mountains of *** species-specific investigations showed that the Sichuan hot-spring snake is still restricted to geothermal zones at elevations higher than 3000 m *** species also remains at risk of extinction,with the effective population size showing continual decline over the past one million years based on coalescent inference from genome-level single nucleotide polymorphism(SNP)*** propose several approaches for protecting Sichuan hot-spring snake populations and their habitat.

关键词： spring snake extinction

Optical detection method of discharge mode transition of inductively coupled plasma in an atmosphere-breathing electric propulsion system

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Chinese Journal of Aeronautics 2024年第10期37卷 172-183页

作者： Yuxuan zhong Yu ZHANG Jianjun WU peng ZHENG College of Aerospace Science and Engineering National University of Defense TechnologyChangsha 410073China

Plasma discharge stability is an important problem in atmosphere-breathing electric propulsion system when maintaining long-term missions at ultra-low earth *** paper designed an inductively coupled plasma source to imitate the ionization *** effect of inflow rate and Radio Frequency(RF)power on the plasma discharge mode transition is experimentally studied.A discharge mode detection method is proposed,which determines the discharge mode by identifying the morphology of the plasma *** using the method,the discharge mode transition is quantified and a control model based on the parameter sensitivity is *** verify the method,the spectra are measured and the electron temperature spatial distribution is *** the method has been proven *** results show that the inductively coupled discharge contains capacitive components affected by the mass flow rate and the radio frequency *** plasma characteristics can be maintained stably by controlling the radio frequency power when the mass flow rate randomly changes in a certain *** is demonstrated that the application of detection method effectively identifies the discharge mode,which is a promising active control method for the plasma discharge mode.

关键词： Atmosphere-breathing electric propulsion Inductively coupled plasma Discharge mode transition Optical diagnosis Ultra-low earth orbit

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医疗服务价格结构与调整策略研究

医院管理论坛 2024年第8期41卷 14-17页

作者：寿敏燕彭忠英吴燕婷嘉兴市妇幼保健院

医疗服务价格调整是深化医药服务供给侧改革的重要手段,科学合理的医疗服务价格是反映医务人员劳动价值、促使公立医院回归公益性的重要保障。由于我国历史的局限以及医疗服务价格改革具有难、繁、重等特点,当前医疗服务价格仍存在诸多... 详细信息

医疗服务价格调整是深化医药服务供给侧改革的重要手段,科学合理的医疗服务价格是反映医务人员劳动价值、促使公立医院回归公益性的重要保障。由于我国历史的局限以及医疗服务价格改革具有难、繁、重等特点,当前医疗服务价格仍存在诸多问题亟待解决。DRG支付方式改革的全面推进为医疗服务价格改革路径探索提供了新的契机。文章通过对J市医疗服务价格改革历程回顾和现状分析,以及对DRG支付政策与医疗服务价格改革协同关系研究,从改革联动、成本核算、信息赋能等方面提出医疗服务价格结构调整路径,以期能为我国的医疗服务价格改革提供参考。

关键词：医疗服务价格价格改革 DRG支付

MgO−V_(2)O_(5)二元系固相反应相变及产物溶解行为

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Transactions of Nonferrous Metals Society of China 2024年第6期34卷 1994-2006页

作者：向俊一罗明帅白路伟陆曦朱忠棚黄青云朱奎松裴贵尚吕学伟重庆科技大学冶金与材料工程学院重庆401331 攀枝花学院钒钛学院攀枝花617000 重庆大学材料科学与工程学院重庆400044

研究不同摩尔比MgO−V_(2)O_(5)混合物的固相反应行为。采用等温溶解平衡法测定25~55℃下反应产物(钒酸镁)在水中的溶解度,并研究其在稀硫酸中的溶解行为和动力学。结果表明,MgO与V_(2)O_(5)的摩尔比和焙烧温度对固相反应产物的相变有较... 详细信息

研究不同摩尔比MgO−V_(2)O_(5)混合物的固相反应行为。采用等温溶解平衡法测定25~55℃下反应产物(钒酸镁)在水中的溶解度,并研究其在稀硫酸中的溶解行为和动力学。结果表明,MgO与V_(2)O_(5)的摩尔比和焙烧温度对固相反应产物的相变有较大影响;MgV_(2)O_(6)在水中溶解度最大,其次是Mg_(2)V_(2)O_(7)和Mg_(3)V_(2)O_(8);随着pH值从4.0降低到2.5,温度从30℃增加到70℃,钒酸镁在稀硫酸中的溶解率显著增加,其在稀硫酸中的溶解符合二级伪均相反应模型。

关键词：提钒 MgO V_(2)O_(5) 焙烧浸出溶解度动力学

归芪益母汤酶解提取物对小鼠生长性能、免疫抗氧化功能和血清生化指标的影响

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饲料工业 2024年第13期45卷 99-104页

作者：刘雪松张艳孟维珊薛沾枚陈曦王爽杨旭东钟鹏黑龙江省农业科学院畜牧兽医分院黑龙江齐齐哈尔161005 黑龙江省兽用药物重点实验室黑龙江齐齐哈尔161005

试验旨在研究归芪益母汤酶解提取物对小鼠生长性能、免疫抗氧化功能和血清生化指标的影响。试验选取4周龄ICR小鼠100只,随机分为1个对照组和4个试验组(A1、A2、A3、A4组)。对照组饲喂基础日粮,A1、A2、A3、A4组分别在基础日粮中添加250... 详细信息

试验旨在研究归芪益母汤酶解提取物对小鼠生长性能、免疫抗氧化功能和血清生化指标的影响。试验选取4周龄ICR小鼠100只,随机分为1个对照组和4个试验组(A1、A2、A3、A4组)。对照组饲喂基础日粮,A1、A2、A3、A4组分别在基础日粮中添加250、500、750、1 000 mg/kg归芪益母汤酶解提取物,每组4个重复,每个重复5只小鼠,预试期3 d,正试期28 d。试验期间,测定小鼠的生长性能、免疫抗氧化功能及血清生化指标。结果显示:与对照组相比,A3、A4组小鼠末重、平均日增重显著提高(P0.05),A2、A3、A4组小鼠血清中三酰甘油、胆固醇、低密度脂蛋白胆固醇含量显著下降(P<0.05),高密度脂蛋白胆固醇含量显著提高(P<0.05);与对照组相比,A2、A3、A4组小鼠肝脏匀浆液中免疫球蛋白A(IgA)、免疫球蛋白G(IgG)、免疫球蛋白M(IgM)、白细胞介素-17(IL-17)、肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)含量显著上升(P<0.05),白细胞介素-2(IL-2)含量显著下降(P<0.05)。综上所述,在日粮中添加归芪益母汤酶解提取物可显著提高小鼠生长性能,增强机体免疫抗氧化功能,有益于机体的脂类代谢。基于饲喂成本考虑,建议在日粮中添加750 mg/kg的归芪益母汤酶解提取物。

关键词：归芪益母汤组方生长性能免疫抗氧化血清生化

Prediction of vertical PM2.5 concentrations alongside an elevated expressway by using the neural network hybrid model and generalized additive model

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Frontiers of Earth Science 2017年第2期11卷 347-360页

作者： Ya GAO Zhanyong WANG Qing-Chang LU Chao LIU zhong-Ren peng Yue YU Center for ITS and UAV Applications Research State Key Laboratory of Ocean Engineering School of Naval Architecture Ocean and Civil Engineering Shanghai Jiao Tong University Shanghai 200240 China Department of Urban and Regional Planning University of Florida FL 32611-5706 USA Department of Electrical and Systems Engineering University of Pennsylvania PA 19104-6314 USA

A study on vertical variation of PM2.5 concentrations was carried out in this paper. Field measurements were conducted at eight different floor heights outside a building alongside a typical elevated expressway in downtown Shanghai, China. Results show that PM2.5 concentration decreases significantly with the increase of height from the 3rd to 7th floor or the 8th to 15th floor, and increases suddenly from the 7th to 8th floor which is the same height as the elevated expressway. A non-parametric test indicates that the data of PM2.5 concentration is statistically different under the 7th floor and above the 8th floor at the 5% significance level. To investigate the relationships between PM2.5 concentration and influencing factors, the Pearson correlation analysis was performed and the results indicate that both traffic and meteorological factors have crucial impacts on the variation of PM2.5 concentration, but there is a rather large variation in correlation coefficients under the 7th floor and above the 8th floor. Furthermore, the back propagation neural network based on principal component analysis （PCA-BPNN）, as well as generalized additive model （GAM）, was applied to predict the vertical PM2.5 concentration and examined with the field measurement dataset. Experimental results indicated that both models can obtain accurate predictions, while PCA-BPNN model provides more reliable and accurate predictions as it can reduce the complexity and eliminate data co-linearity. These findings reveal the vertical distribution of PM2.5 concentration and the potential of the proposed model to be applicable to predict the vertical trends of air pollution in similar situations.

关键词： vertical variations principal component ana-lysis back propagation neural network generalized additivemodel urban elevated expressway

Magnetohydrodynamics (MHD) numerical simulations on the interaction of the solar wind with the magnetosphere: A review

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Science China Earth Sciences 2013年第7期56卷 1141-1157页

作者： WANG Chi GUO XiaoCheng peng zhong TANG BinBin SUN TianRan LI WenYa HU YouQiu State Key Laboratory of Space Weather Center for Space Science and Applied Research Chinese Academy of Sciences School of Earth and Space Sciences University of Science and Technology of China

The magnetosphere is the outermost layer of the geospace, and the interaction of the solar wind with the magnetosphere is the key element of the space weather cause-and-effect chain process from the Sun to Earth, which is one of the most challenging scientific problems in the geospace weather study. The nonlinearity, multiple component, and time-dependent nature of the geospace make it very difficult to describe the physical process in geospace using traditional analytic analysis approach. Numerical simulations, a new research tool developed in recent decades, have a deep impact on the theory and application of the geospace. MHD simulations started at the end of the 1970s, and the initial study was limited to two-dimensional (2D) cases. Due to the intrinsic three-dimensional (3D) characteristics of the geospace, 3D MHD simulations emerged in the 1980s, in an attempt to model the large-scale structures and fundamental physical processes in the magnetosphere. They started to combine with the space exploration missions in the 1990s and make comparisons with observations. Physics-based space weather forecast models started to be developed in the 21st century. Currently only a few space-power countries such as USA and Japan have developed 3D magnetospheric MHD models. With the rapid advance of space science in China, we have developed a new global MHD model, namely PPMLR-MHD, which has high order spatial accuracy and low numerical dissipation. In this review, we will briefly introduce the global 3D MHD modeling, especially the PPMLR-MHD code, and summarize our recent work based on the PPMLR-MHD model, with an emphasis on the interaction of interplanetary shocks with the magnetosphere, large-scale current systems, reconnection voltage and transpolar potential drop, and Kelvin-Helmholtz (K-H) instability at the magnetopause.

关键词： solar wind magnetosphere MHD simulations

Timetable optimization for single bus line based on hybrid vehicle size model

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Journal of Traffic and Transportation Engineering(English Edition) 2015年第3期2卷 179-186页

作者： Daniel(Jian) Sun Ya Xu zhong-Ren peng State Key Laboratory of Ocean Engineering Shanghai Jiao Tong University Key Laboratory of Highway Engineering of Ministry of Education Changsha University of Science & Technology Transportation Research Center Shanghai Jiao Tong University Department of Urban and Regional Planning University of Florida

This study proposes a flexible timetable optimization method based on hybrid vehicle size model to tackle the bus demand fluctuations in transit operation. Three different models for hybrid vehicle, large vehicle and small vehicle are built in this study, respectively. With the operation data of Shanghai Transit Route 55 at peak and off-peak hours, a heuristic algorithm was proposed to solve the problem. The results indicate that the hybrid vehicle size model excels the other two modes both in the total time and total cost. The study verifies the rationality of the strategy of hybrid vehicle size model and highlights the importance of the adaptive vehicle size in dealing with the bus demand fluctuation. The main innovation of the study is that unlike traditional timetables, the arrangement of the scheduling interval and the corresponding bus type or size are both involved in the timetable of hybrid vehicle size bus mode, which will be more effective to solve the problem of passenger demand fluctuation. Findings from this research would provide a new perspective to improve the level of regular bus service.

关键词： Public transport Timetable optimization Hybrid size bus Bus operation