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Moxibustion of Zusanli(ST36)and Shenshu(BL23)alleviates the inflammation of rheumatoid arthritis in rats through regulating macrophage migration inhibitory factor/glucocorticoids signaling

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Journal of Traditional Chinese Medicine 2024年第2期44卷 353-361页

作者： ZHANG Linlin zhong yumei LU Wenting SHANG Yanan GUO Yanding LUO Xiaochao CHEN Yang LUO Kun HU Danhui YU Huiling ZHOU Haiyan Acupuncture and Moxibustion College Chengdu University of Traditional Chinese MedicineChengdu 610075China Department of Painology First People's Hospital of ChengduChengdu 610095China Chinese Evidence-Based Medicine Centre West China HospitalSichuan UniversityChengdu 610044China Traditional Chinese Medicine College Chongqing Medical UniversityChongqing 400016China External treatment center First People's Hospital of ChengduChengdu 610095China

OBJECTIVE:To test the hypothesis that moxibustion may inhibit rheumatoid arthritis(RA)synovial inflammation by regulating the expression of macrophage migration inhibitory factor(MIF)/glucocorticoids(GCs).METHODS:Fifty male Sprague-Dawley rats were randomly divided into five groups(n=10 each):blank Control(CON)group,RA Model(RA)group,Moxibustion(MOX)group,MIF inhibitor(S,R)-3-(4-hydroxyphenyl)-4,5-dihydro-5-isoxazole acetic acid methyl ester(ISO-1)group,and Moxibustion+MIF inhibitor ISO-1(MOX+ISO-1)group.Rats in the ISO-1 group and ISO-1+MOX group were intraperitoneally injected with the inhibitor ISO-1.The rats in the RA group,ISO-1 group,MOX group,and ISO-1+MOX group were injected with Freund's complete adjuvant(FCA)in the right hind footpad to establish an experimental RA rat model.In the MOX group and MOX+ISO-1 group,rats were treated with Moxa.The thickness of the footpads of the rats in each group was measured at three-time points before,after modeling and after moxibustion treatment.The contents of serum MIF,corticosterone(CORT),tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)were detected by enzyme-linked immunosorbent assay;and the contents of synovial MIF were detected by Western blot.Hematoxylin-eosin(HE)staining method was used to observe the pathological changes of synovial tissue under a section light microscope,and pathological scoring was performed according to the grading standard of the degree of synovial tissue disease.RESULTS:Moxibustion was found to reduce the level of MIF and alleviate inflammation in RA rats in this study.In addition,after inhibiting the expression of MIF,the level of CORT increased,and the level of TNF-α decreased.Treating RA rats with inhibited MIF by moxibustion,the level of CORT was almost unchanged,but the level of TNF-α further decreased.The correlation analysis data suggested that MIF was positively related to the expression of TNF-α and negatively correlated with the expression of CORT.CONCLUSION:Reducing MIF to increase CORT and decrease TNF-α by moxibustion

关键词： rheumatoid arthritis macrophage migration inhibitory factor moxibustion corticosterone glucocorticoids

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Study on the anti-inflammatory mechanism of moxibustion in rheumatoid arthritis in rats based on phospholipaseA2 signaling inhibition by Annexin 1

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Journal of Traditional Chinese Medicine 2024年第4期 753-761页

作者： GUO Yanding LUO Kun ZHANG Linlin LU Wenting SHANG Yanan zhong yumei HU Danhui YANG Xin ZHOU Haiyan Department of Acupuncture-Moxibustion and Tuina the Third Affiliated HospitalChengdu University of Traditional Chinese Medicine External Treatment Center First People's Hospital of Chengdu Department of Chengdu Integrated Traditional Chinese Medicine and Western Medicine Hospital Department of Health Rehabilitation School Chengdu University of Traditional Chinese Medicine

OBJECTIVE: To determine whether moxibustion had an anti-inflammatory effect on rheumatoid arthritis(RA) by regulating Annexin 1 expression and interfering with the phospholipaseA2 signaling pathway. METHODS: Thirty male Sprague-Dawley rats were randomly categorized into five groups(six rats per group): blank control(CON) group, RA model(RA) group, moxibustion(MOX) group, Annexin 1 lentiviral intervention(RNAi-Anxa1) group, and Annexin 1 lentiviral intervention + moxibustion(RNAi-Anxa1 + MOX) group. The rats in the RNAi-Anxa1 and the RNAi-Anxa1 + MOX groups were injected with the lentiviral vector-mediated RNAi-Anxa1 into the rat foot pad. An experimental RA rat model was established by injecting Freund's complete adjuvant(FCA) into the RA, MOX, RNAi-Anxa1, and RNAi-Anxa1 + MOX groups. Rats in the MOX and RNAiAnxa1 + MOX groups received moxibustion treatment. After modeling, using moxibustion “Shenshu(BL23)” and “Zusanli(ST36)”, each point is 5 times, bilateral alternating, once a day, 6 times for a course of treatment, between the courses of rest for a one day. A total of three treatment courses were conducted. Both bilateral pad thicknesses were measured using Vernier calipers on experimental days 1, 7, 14, 21, and 28. The expression of cPLA2α signaling in the synovium of diseased joints was observed using Western blot. The pathology of the rat ankle synovium was observed using hematoxylineosin(HE) staining. Interleukin(IL)-1β, IL-10, prostaglandin E2(PGE2), and leukotriene B4(LTB4) were detected using enzyme-linked immunosorbent assay. RESULTS: Moxibustion increased the levels of Annexin 1 and decreased the inflammatory response in rats with RA. After increasing the expression of Annexin 1, the phosphorylated expression of cPLA2α was inhibited, the serum levels of IL-1β, PGE2, and LTB4 decreased, and the level of IL-10 increased. In moxibustion treated RA rats after the Annexin 1 lentiviral intervention, the serum levels of IL-1β, PGE2, LTB4, and IL-10 were almost unchanged. CONCLUSION: Moxibustion enhanced the

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Efficacy of Moxa-burning heat stimulating Zusanli(ST36) and Shenshu(BL23) on expressions of macrophage migration inhibitory factor and macrophage apoptosis in rabbits with adjuvant-induced arthritis

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Journal of Traditional Chinese Medicine 2022年第6期42卷 980-987页

作者： ZHOU Haiyan zhong yumei GAO Xiuhua WU Fei JIA Min YANG Xin School of Acupuncture-Moxibustion and Tuina Chengdu University of Traditional Chinese MedicineChengdu 610075China Pain Department Chengdu First People's Hospital/Chengdu Integrated TCM&Western Medicine HospitalChengdu 610095China Centre of Preventive Treatment of Disease Teaching Hospital of Chengdu University of Traditional Chinese MedicineChengdu 610075China Foreign Languages School Chengdu University of Traditional Chinese MedicineChengdu 610075China Acupuncture Department Second Affiliated Hospital of Baotou Medical CollegeBaotou 014030China Health Rehabilitation School Chengdu University of Traditional Chinese MedicineChengdu 610075China

OBJECTIVE: To evaluate the effects of moxa-burning heat stimulating acupoints Zusanli(ST36) and Shenshu(BL23) on macrophage migration inhibitory factor(MIF) and its related molecules which can provide scientific experimental basis for the clinical application of moxibustion treatment of rheumatoid arthritis(RA). METHODS: Thirty rabbits were randomly assigned to control group, RA model(established by injecting Freund’s Complete Adjuvant) group(RA group) and RA model with moxibustion group [Moxa group, Zusanli(ST36) and Shenshu(BL23), 5 moxa pillars/day, 6 d × 3]. The expressions of MIF m RNA were evaluated with reverse transcription polymerase chain reaction;the apoptosis rates of macrophages were detected by erminal deoxynucleotidyl transferase-mediated d TUP nick end labeling;the expressions of related signal molecules were detected with immunohistochemical S-P method and the levels of IL-2 were detected with enzymelinked immunosorbent assay. RESULTS: The expressions of MIF m RNA, extracellular regulated protein kinases 2, p38 mitogen-activated protein kinase and nuclear factor-κ-gene binding p65 in synovial tissue of RA group were significantly increased when compared with control group, which were lower remarkably in moxa group than those in RA group. The apoptosis rates of macrophages in RA group were significantly down-regulated as compared with the control group, which were up-regulated in moxa group compared with the RA group. The levels of IL-2 in synovial fluid from the RA group were elevated significantly as compared with that from control group, but those of the moxa group were reduced when compared with those from RA group. CONCLUSIONS: Moxibustion may simultaneously regulate the expressions of MIF and its related signaling pathways molecules, the apoptosis rate of macrophages in synovial tissue, as well as the level of inflammatory factors in synovial fluid. The results suggest that the antiinflammatory effect of moxibustion on RA may be related to inhibit the expression of MIF in synovial tissue, th

关键词： rheumatoid arthritis moxibustion macrophage migration-inhibitory factors macrophages

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“四史”教育融入“习近平新时代中国特色社会主义思想概论”课程的途径探索

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福建开放大学学报 2024年第3期 60-63页

作者：钟玉梅福建信息职业技术学院福建福州350003

“习近平新时代中国特色社会主义思想概论”课程是重要的思政课程,对于提高思政育人水平,增强学生思政素养,促进学生发展具有不可或缺的价值。文章旨在探讨“四史”教育融入“习近平新时代中国特色社会主义思想概论”课程的有效途径。首... 详细信息

“习近平新时代中国特色社会主义思想概论”课程是重要的思政课程,对于提高思政育人水平,增强学生思政素养,促进学生发展具有不可或缺的价值。文章旨在探讨“四史”教育融入“习近平新时代中国特色社会主义思想概论”课程的有效途径。首先,揭示了“四史”教育融入课程对于感悟理想信念、摒弃历史虚无主义、厚植爱国主义情怀的重要意义。其次,从内容建设层面,提出将历史发展脉络、生动素材和实践经验融入思政课程,以提升教学层次、温度与深度。最后,提出创新教学模式、强化教师队伍建设、加强顶层设计和制度建设等具体措施,以科学规范思想政治育人工作。通过这些途径的探索与实践,可有效推动“四史”教育与思政课程的深度融合,提升思政育人实效性,培养出具备坚定信念、深厚历史素养和使命感的时代新人。

关键词： “四史”教育习近平新时代中国特色社会主义思想融入思政教育

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不同方法制备的海带褐藻糖胶得率及品质的比较

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食品工业科技 2024年第14期45卷 194-203页

作者：钟玉美王雷许加超高昕付晓婷海洋食品加工与安全控制全国重点实验室中国海洋大学食品科学与工程学院山东青岛266000

为建立褐藻中活性多糖褐藻糖胶的标准制备工艺,本研究以海带为原料,选择高温高压提取、酸提取、微波提取(M400 W、M500 W、M600 W)3种方法,分别使用氯化钙沉淀(CP)和乙醇沉淀(GAP)脱除褐藻胶,再使用高浓度乙醇进行醇沉,得到褐藻糖胶。... 详细信息

为建立褐藻中活性多糖褐藻糖胶的标准制备工艺,本研究以海带为原料,选择高温高压提取、酸提取、微波提取(M400 W、M500 W、M600 W)3种方法,分别使用氯化钙沉淀(CP)和乙醇沉淀(GAP)脱除褐藻胶,再使用高浓度乙醇进行醇沉,得到褐藻糖胶。比较了10种提取条件下海带褐藻糖胶的得率,对提取得到的褐藻糖胶的总糖、总酚、蛋白质、糖醛酸、硫酸基含量进行测定,并对其Fe3+还原能力及ABTS+、DPPH自由基清除能力进行研究,并对上述测定结果进行相关性分析,筛选出高得率、高纯度、抗氧化活性好的褐藻糖胶制备方法。结果表明,酸提取-钙沉(AE-CP)和微波提取-钙沉(M400 W-CP、M500 W-CP、M600 W-CP)褐藻糖胶得率分别为3.821%、3.516%、3.376%、2.998%,硫酸基含量分别为17.734%、23.872%、23.376%、22.149%,蛋白质和总酚含量均在1%以下,糖醛酸含量低于10%,Trolox当量(ABTS+自由基清除能力)分别为5.637、5.336、6.567、5.616μg/mg,均为理想的褐藻糖胶制备方法。其中,微波提取-钙沉法具有耗时短、溶剂用量少的特点,符合绿色加工的要求。本研究系统地考察了褐藻糖胶制备方法对其得率和品质的影响,建立了高得率、高纯度、高抗氧化活性褐藻糖胶的制备工艺。

关键词：海带褐藻糖胶钙沉分级醇沉抗氧化活性

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小鼠骨髓和脂肪间充质干细胞定向分化能力的比较研究

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天津医药 2024年第2期52卷 129-135页

作者：钟家帅冯玉梅天津医科大学肿瘤医院国家恶性肿瘤临床医学研究中心天津市恶性肿瘤临床医学研究中心天津市肿瘤防治重点实验室天津医科大学肿瘤医院肿瘤研究所生物化学与分子生物学研究室300060

目的探讨小鼠骨髓源性间充质干细胞(BM-MSCs)和脂肪源性间充质干细胞(AD-MSCs)的定向分化能力。方法从C57BL/6J小鼠股骨骨髓和腹股沟白色脂肪组织中分别分离和培养BM-MSCs和AD-MSCs,分别使用成骨、成软骨和成脂诱导分化培养基诱导两种... 详细信息

目的探讨小鼠骨髓源性间充质干细胞(BM-MSCs)和脂肪源性间充质干细胞(AD-MSCs)的定向分化能力。方法从C57BL/6J小鼠股骨骨髓和腹股沟白色脂肪组织中分别分离和培养BM-MSCs和AD-MSCs,分别使用成骨、成软骨和成脂诱导分化培养基诱导两种细胞定向分化。采用茜素红、阿利新蓝和油红O染色检测成骨、成软骨和成脂分化程度;实时荧光定量PCR(q PCR)鉴定MSCs并检测定向分化相关基因Runx2、Sp7(成骨),Sox9、Col2a1(成软骨),Pparg和Cebpa(成脂)表达水平,确定细胞的定向分化能力。基于GEO数据库中GSE43804和GSE122778数据集的小鼠和人类BM-MSCs和AD-MSCs基因表达谱数据,分析差异表达基因及其富集的信号通路。结果分离培养得到的BM-MSCs和AD-MSCs细胞形态不同,AD-MSCs梭形形态更明显;两种细胞均表达CD29、CD44和CD90,不表达CD34和CD45。定向诱导后AD-MSCs的成骨和成脂分化程度高于BM-MSCs,而成软骨分化程度低于BM-MSCs(P<0.05);定向诱导后AD-MSCs中Runx2、Pparg和Cebpa mRNA表达水平高于BM-MSCs,Sox9 mRNA表达水平低于BM-MSCs(P<0.05)。小鼠和人的AD-MSCs高表达的基因富集于PPAR和WNT信号通路,BM-MSCs高表达的基因富集于软骨和骨发育信号通路。结论小鼠AD-MSCs成骨和成脂分化能力强于BM-MSCs,而成软骨分化能力弱于BM-MSCs,PPAR、WNT、软骨和骨发育信号通路的活化状态在决定BM-MSCs和AD-MSCs不同定向分化潜能中起重要调节作用。

关键词：间质干细胞骨髓脂肪类 PPARγ Wnt信号通路成骨分化成软骨分化成脂分化

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正念疗法对抑郁症实施无抽搐电休克治疗患者应激反应的影响

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中国当代医药 2024年第11期31卷 164-167页

作者：李玉梅钟国锋冯肖玲广东省茂名市第三人民医院护理部广东茂名525200 广东省茂名市第三人民医院门诊部广东茂名525200

目的探索正念疗法干预下抑郁症实施无抽搐电休克治疗(MECT)患者应激反应的改善效果。方法选取2022年7月至2023年4月茂名市第三人民医院收治的96例抑郁症实施MECT的患者作为研究对象,采用随机数字表法将其分为研究组(48例)和对照组(48例... 详细信息

目的探索正念疗法干预下抑郁症实施无抽搐电休克治疗(MECT)患者应激反应的改善效果。方法选取2022年7月至2023年4月茂名市第三人民医院收治的96例抑郁症实施MECT的患者作为研究对象,采用随机数字表法将其分为研究组(48例)和对照组(48例)。对照组采用治疗前常规护理,研究组采用治疗前常规护理联合正念疗法干预。入院当日、治疗前30 min、治疗后2 h监测患者的心率(HR)和收缩压(SBP),在干预前和治疗前30 min采用焦虑自评量表(SAS)、汉密尔顿焦虑量表(HAMA)测量焦虑水平,比较两组患者应激反应和焦虑状态改善效果。结果干预前,两组患者的HR、SBP、SAS及HAMA评分比较,差异无统计学意义(P>0.05);干预后,研究组患者治疗前30 min、治疗后2 h的HR、SBP和治疗前30 min的SAS、HAMA均低于对照组,差异有统计学意义(P<0.05)。结论正念疗法的运用能明显改善抑郁症实施MECT患者的应激反应和焦虑状态,有利于MECT顺利进行,值得推广应用。

关键词：正念疗法抑郁症无抽搐电休克治疗应激反应

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The development road of ammonium phosphate fertilizer in China

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Chinese Journal of Chemical Engineering 2022年第1期35卷 170-175页

作者： Dehua Xu Benhe zhong Xinlong Wang Xue Li Yanjun zhong Zhengjuan Yan Jingxu Yang Xiaobin Li yumei Wang Xiaohou Zhou Ministry of Education Research Center for Comprehensive Utilization and Clean Processing Engineering of Phosphorus Resources College of Chemical EngineeringSichuan UniversityChengdu 610065China

Ammonium phosphate fertilizer is the compounds containing nitrogen and phosphorus that are usually produced through the neutralization reaction of phosphoric acid and ammonia.At present,there are a variety of products,such as slurry monoammonium phosphate(MAP),diammonium phosphate(DAP),industrial grade MAP,water soluble MAP,water soluble ammonium polyphosphate(APP)and so on.After more than 60 years of development,China’s ammonium phosphate fertilizer industry has experienced the road of from scratch and from weak to strong.The successful development of the slurry MAP technology ended the history that the high concentration phosphate fertilizer cannot be produced by using the medium and low grade phosphate ore.The continuous,stable and large-scale production of DAP plant provides sufficient guarantee for DAP products in China.The development of new ammonium phosphate fertilizer products,such as industrial grade MAP,water soluble MAP,water soluble APP,provides technical support for the transformation and upgrading of phosphorus chemical enterprises.In this paper,the production methods,the development history and the latest research progress of ammonium phosphate fertilizers were reviewed.

关键词： Slurry monoammonium phosphate(MAP) Diammonium phosphate(DAP) Industrial grade MAP Water soluble MAP Water soluble ammonium polyphosphate(APP)

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基于改进YOLOv5的病死猪猪头的识别及三维定位方法

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江西农业大学学报 2024年第3期46卷 763-773页

作者：彭兴鹏何秀文孙云涛刘仁鑫梁亚茹钟玉媚庞佳熊康文江西农业大学江西省畜牧设施研究中心江西南昌330045

【目的】旨在为病死猪搬运机器人提供抓取目标,提出一种基于改进YOLOv5的病死猪猪头的识别及三维定位方法。【方法】将YOLOv5目标检测算法主干特征提取网络(backbone)替换成轻量化特征提取网络MobileNetV2,降低所得训练权重参数大小;在... 详细信息

【目的】旨在为病死猪搬运机器人提供抓取目标,提出一种基于改进YOLOv5的病死猪猪头的识别及三维定位方法。【方法】将YOLOv5目标检测算法主干特征提取网络(backbone)替换成轻量化特征提取网络MobileNetV2,降低所得训练权重参数大小;在主干特征提取网络中引入CBAM注意力机制来提高对病死猪猪头的关注度;使用RealsenseD435深度相机获取目标图像,建立针对病死猪猪头的三维空间坐标的成像模型;并设计对比试验与定位试验对其进行验证。【结果】相较于YOLOv5特征提取网络,轻量化处理主干网络能使权重文件大小从13.7 MB下降到5.9 MB,降幅达到56%;CBAM注意力机制的引入使算法单张图片的检测速度从17.9 ms下降到11.6 ms,减少6.3 ms;RealsenseD435深度相机构造的三维定位模型在X,Y,Z轴方向上的平均误差分别为0.021,0.023,0.042 m,均小于0.05 m。【结论】改进的YOLOv5目标检测模型能有效降低权值文件大小,提高检测速率。RealsenseD435深度相机构建的三维定位模型能够准确定位到病死猪头部,并计算出其三维空间坐标。所以基于改进YOLOv5的病死猪猪头的识别及三维定位方法,满足病死猪搬运机器人的识别定位要求。

关键词： YOLOv5 病死猪猪头识别三维定位注意力机制无人化

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基于SPME-GC-MS的杏子提取物挥发性成分分析及其应用

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农产品加工 2024年第4期 76-79页

作者：钱玉梅范忠农李政施怡黄东业广西中烟工业有限责任公司广西南宁530001

采用固相微萃取结合GC-MS技术对杏子提取物的挥发性成分进行分析鉴定。结果表明,从杏子提取物中共鉴定出50种挥发性成分,其主要成分为香茅醛(17.28%)、柠檬烯(11.04%)、丁香酚(7.64%)、香茅醇(7.25%)、香芹酮(6.28%)等物质构成了杏子提... 详细信息

采用固相微萃取结合GC-MS技术对杏子提取物的挥发性成分进行分析鉴定。结果表明,从杏子提取物中共鉴定出50种挥发性成分,其主要成分为香茅醛(17.28%)、柠檬烯(11.04%)、丁香酚(7.64%)、香茅醇(7.25%)、香芹酮(6.28%)等物质构成了杏子提取物的主体香韵,能够增加卷烟果香、甜香、膏香、清香香韵,降低卷烟的辛辣感和刺激性,提升烟气品质,改善余味,提高卷烟吸食品质。

关键词：杏子提取物挥发性成分固相微萃取气相色谱-质谱联用法卷烟

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