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Genomic profiling of colorectal cancer in large-scale Chinese patients:amplification and somatic mutations in ERBB2

Oncology Research 2024年第9期32卷 1429-1438页

作者： YUZHI LIU EVELYNE BISCHOF zhiqin chen JIAHUAN ZHOU BEI ZHANG DING ZHANG YONG GAO MING QUAN Department of Oncology Shanghai East HospitalTongji University School of MedicineShanghaiChina State Key Laboratory of Oncogenes and Related Genes Shanghai Cancer InstituteDepartment of OncologyRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina Jiangsu Hengrui Pharmaceuticals Co. Ltd.ShanghaiChina D Medicines Inc. ShanghaiChina

Objectives:Human epidermal growth factor receptor 2(HER2)-targeted therapies have demonstrated potential benefits for metastatic colorectal cancer(mCRC)patients with HER2 amplification,but are not satisfactory in cases of HER2 mutant ***:Consequently,further elucidation of amplifications and somatic mutations in erythroblastic oncogene B-2(ERBB2)is *** genomic profiling was conducted on 2454 Chinese CRC cases to evaluate genomic alterations in 733 cancer-related genes,tumor mutational burden,microsatellite instability,and programmed death ligand 1(PD-L1)***:Among 2454 CRC patients,85 cases(3.46%)exhibited ERBB2 amplification,and 55 cases(2.24%)carried ERBB2 mutation.p.R678Q(28%),p.V8421(24%),and p.S310F/Y(12%)were the most prevalent of the 16 detected mutation *** comparison to the ERBB2 altered(alt)group,KRAS/BRAF mutations were more prevalent in ERBB2 wild-type(wt)samples(ERBB2wt ***2alt,KRAS:50.9%vs.25.6%,p<0.05;BRAF:8.5%vs.2.3%,p<0.05).32.7%(18/55)of CRCs with ERBB2 mutation exhibited microsatellite instability high(MSI-H),while no cases with HER2 amplification displayed *** genes varied between ERBB2 copy number variation(CNV)and ERBB2 single nucleotide variant(SNV);TP53 alterations tended to co-occur with ERBB2 amplification(92.3%)as opposed to ERBB2 mutation(58.3%).KRAS and PIK3CA alterations were more prevalent in ERBB2 SNV cases(KRAS/PIK3CA:45.8%/31.2%)compared to ERBB2 amplification cases(KRAS/PIK3CA:14.1%/7.7%).Conclusion:Our study delineates the landscape of HER2 alterations in a large-scale cohort of CRC patients from *** findings enhance our understanding of the molecular features of Chinese CRC patients and offer valuable implications for further investigation.

关键词： Colorectal cancer ERBB2 HER2 Human epidermal growth factor receptor 2 Genetic profiling Precision oncology

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MET变异型消化道肿瘤的诊治进展

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肿瘤研究与临床 2024年第2期36卷 146-149页

作者：陈晨陈智琴高勇权明同济大学附属东方医院肿瘤科上海200123

消化道肿瘤的发病率日益升高且呈年轻化的趋势。随着精准医学概念的提出,通过特异性阻断肿瘤细胞信号转导,杀伤肿瘤细胞的分子靶向治疗逐步成为消化道肿瘤治疗的热点。文章以MET变异型消化道肿瘤为切入点,概述相关临床特征及分子机制,... 详细信息

消化道肿瘤的发病率日益升高且呈年轻化的趋势。随着精准医学概念的提出,通过特异性阻断肿瘤细胞信号转导,杀伤肿瘤细胞的分子靶向治疗逐步成为消化道肿瘤治疗的热点。文章以MET变异型消化道肿瘤为切入点,概述相关临床特征及分子机制,并总结了消化道肿瘤MET靶向治疗的最新进展。

关键词：消化道肿瘤细胞间质上皮转换因子靶向疗法

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土壤重金属无机钝化剂及其有机改良化钝化剂对稻田镉钝化和温室气体排放的影响

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环境科学学报 2024年第5期44卷 435-445页

作者：邹彤林强陈志琴连加攀杨肖娥浙江大学环境与资源学院污染环境修复与生态健康教育部重点实验室杭州310058

为探究土壤重金属无机钝化剂及其有机改良化钝化剂对稻田镉钝化和温室气体排放的综合影响,本研究通过水稻盆栽实验,实施了空白对照组、施加1%无机钝化剂(石灰∶海泡石=1∶1)组、施加1%无机钝化剂和0.5%生物炭组以及施加1%无机钝化剂和0... 详细信息

为探究土壤重金属无机钝化剂及其有机改良化钝化剂对稻田镉钝化和温室气体排放的综合影响,本研究通过水稻盆栽实验,实施了空白对照组、施加1%无机钝化剂(石灰∶海泡石=1∶1)组、施加1%无机钝化剂和0.5%生物炭组以及施加1%无机钝化剂和0.5%发酵菌糠组4个处理,监测不同处理对镉钝化、温室气体排放、作物产量和土壤质量的影响.结果表明:无机钝化剂显著降低了糙米镉含量43.4%(p<0.05),对稻田温室气体排放无显著影响(p<0.05).生物炭改良和发酵菌糠改良的钝化剂分别降低糙米镉含量的60.3%和46.7%(p<0.05),但同时显著增加了温室气体甲烷的累积排放通量(分别增加30.4%和113.7%,p<0.05).相比于发酵菌糠改良钝化剂,生物炭改良能更显著地提升镉钝化效率并且较低地增加稻田甲烷排放风险.在作物增产和土壤提质方面,生物炭改良钝化剂还具有显著提升水稻籽粒产量、土壤有机碳和速效养分含量的优点.本研究表明,稻田施用无机钝化剂是一种保守且无碳排影响的修复方式,施用生物炭改良钝化剂是一种高效、多效但具低甲烷排放风险的修复方式.

关键词：重金属钝化水稻甲烷二氧化碳无机钝化剂生物炭发酵菌糠

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卵巢储备功能正常患者应用PPOS与激动剂长方案的每取卵周期累积活产率比较

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中华生殖与避孕杂志 2024年第3期44卷 237-248页

作者：陈虹李国华伍园园史文佩陈淼鑫滕晓明夏艳陈智勤同济大学附属上海市第一妇婴保健院生殖医学科上海201204 同济大学附属上海市第一妇婴保健院生殖免疫科上海201204 同济大学附属上海市第一妇婴保健院临床研究中心上海201204 同济大学附属上海市第一妇婴保健院妇科上海201204

目的比较激动剂长方案和高孕激素状态下促排卵(progestin-primed ovarian stimulation,PPOS)方案在卵巢储备功能正常患者中的每取卵周期累积活产率。方法回顾性队列研究分析了2017年1月至2019年12月期间在同济大学附属上海市第一妇婴保... 详细信息

目的比较激动剂长方案和高孕激素状态下促排卵(progestin-primed ovarian stimulation,PPOS)方案在卵巢储备功能正常患者中的每取卵周期累积活产率。方法回顾性队列研究分析了2017年1月至2019年12月期间在同济大学附属上海市第一妇婴保健院生殖医学科接受体外受精/卵胞质内单精子注射(in vitro fertilization/intracytoplasmic sperm injection,IVF/ICSI)助孕的年龄小于40岁且卵巢储备正常(月经周期正常、卵泡刺激素5)的女性。主要结局指标为促排卵之日起18个月内的累积活产率。结果共995例患者纳入分析,其中使用PPOS方案509例(PPOS组),激动剂长方案486例(激动剂长方案组)。两组患者的基本特征以及临床和实验室指标基本相似,但PPOS组的不孕年限[3(2,4)年]较激动剂长方案组[3(2,5)年]更短(P=0.015)。激动剂长方案组中有372例(77%)进行了鲜胚移植,获得临床妊娠218例、活产197例。新鲜胚胎每移植周期临床妊娠率、持续妊娠率和活产率分别为58.6%(218/372)、54.0%(201/372)和53.0%(197/372),而PPOS组没有进行鲜胚移植。在研究期间,PPOS组共进行冻融胚胎移植(frozen-thawed embryo transfer,FET)662个周期,在激动剂长方案组中共进行257个FET周期。激动剂长方案组FET周期移植周期活产率为42.8%(110/257),显著高于PPOS组[31.1%(206/662),OR=0.727,95%CI:0.607~0.871,P<0.001]。PPOS组在所有FET周期中的胚胎种植率[29.2%(293/1004)]低于激动剂长方案组[34.5%(157/455),OR=0.846,95%CI:0.721~0.992,P=0.041]。在一个完整的IVF/ICSI周期(包括新鲜胚胎及随后所有冷冻胚胎)后,PPOS组在18个月随访期内的累积活产率[40.5%(206/509)]显著低于激动剂长方案组[63.2%(307/486),OR=0.641,95%CI:0.565~0.726,P<0.001]。与PPOS组相比,激动剂长方案组从促排卵到妊娠和活产的平均时间均显著缩短(均P<0.001)。在Kaplan-Meier分析中,激动剂长方案组累积持续妊娠达到活产率显著高于PPOS组(long rank检验,P<0.001)。Cox回归分析调整其他混杂因素后显示采用的刺激方案与累积活产率密切相关(OR=1.917,95%CI:1.152~3.190,P=0.012)。结论在卵巢储备正常的女性中,采用PPOS方案相比传统的激动剂长方案而言累积活产率更低,达妊娠/活产时间更长。

关键词：受精,体外胚胎移植高孕激素状态下促排卵促性腺激素释放激素激动剂长方案累积活产率

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影像组学在预测根治性治疗后前列腺癌生化复发的研究进展

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临床泌尿外科杂志 2024年第7期39卷 636-641页

作者：陈鑫阳陈治钦谢赣生殷虎明李煜李纲苏州大学附属第一医院泌尿外科江苏苏州215031

前列腺癌根治性治疗后,由于肿瘤的特异性,一些高危的前列腺癌患者往往会出现复发的可能。影像组学在前列腺癌的诊断、术后管理有着突出的作用,本文就影像组学在预测前列腺癌生化复发的研究进展进行综述。

关键词：前列腺癌生化复发影像组学深度学习根治性前列腺切除术放射治疗

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Sensitive electrochemiluminescence resonance energy transfer(ECL-RET) between Ru(bpy)3^2＋ and Au nanorod for hydrogen peroxide detection

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Science China Chemistry 2017年第3期60卷 410-414页

作者： Meisheng Wu zhiqin chen Hengyu Xu Aiping Zhang Department of Chemistry College of Science Nanjing Agricultural University Nanjing 210095 China

Here,we developed a novel electrochemiluminescence resonance energy transfer(ECL-RET) approach between Ru(bpy)_3^(2+) and Au nanorods(NRs) for sensitive determination of H_2O_*** NRs were synthesized through silver ion-assisted seed-mediated method which exhibited an obvious absorption peak at about 627 *** were modified at glassy carbon electrode(GCE) surface which showed a significant ECL quenching efficiency about 56.5%due to the ECL-RET *** Au NRs modified electrode was then utilized to measure the concentration of H_2O_2 on the basis of the significant quenching effect of H_2O_2 on Ru(bpy)_3^(2+) *** demonstrated that the decrement of ECL intensity at Au NRs modified electrode had ~ 6.6-fold enhancement as compared with that at bare electrode.

关键词： electrochemiluminescence resonance energy transfer Au NRs

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Irrigation regimes modulate non-structural carbohydrate remobilization and improve grain filling in rice(Oryza sativa L.)by regulating starch metabolism

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Journal of Integrative Agriculture 2024年第5期23卷 1507-1522页

作者： Yuguang Zang Gaozhao Wu Qiangqiang Li Yiwen Xu Mingming Xue Xingyu chen Haiyan Wei Weiyang Zhang Hao Zhang Lijun Liu zhiqin Wang Junfei Gu Jianchang Yang Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Agricultural College YangzhouUniversityYangzhou225009China Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009China

Recently developed ‘super’ rice cultivars with greater yield potentials often suffer from the problem of poor grain filling, especially in inferior spikelets. Here, we studied the activities of enzymes related to starch metabolism in rice stems and grains, and the microstructures related to carbohydrate accumulation and transportation to investigate the effects of different water regimes on grain filling. Two ‘super’ rice cultivars were grown under two irrigation regimes of well-watered(WW) and alternate wetting and moderate soil drying(AWMD). Compared with the WW treatment,the activities of ADP glucose pyrophosphorylase(AGPase), starch synthase(StSase) and starch branching enzyme(SBE), and the accumulation of non-structural carbohydrates(NSCs) in the stems before heading were significantly improved, and more starch granules were stored in the stems in the AWMD treatment. After heading, the activities of α-amylase, β-amylase, sucrose phosphate synthase(SPS) and sucrose synthase in the synthetic direction(SSs)were increased in the stems to promote the remobilization of NSCs for grain filling under AWMD. During grain filling, the enzymatic activities of sucrose synthase in the cleavage direction(SSc), AGPase, StSase and SBE in the inferior spikelets were increased, which promoted grain filling, especially for the inferior spikelets under ***, there were no significant differences in vascular microstructures. The grain yield and grain weight could be improved by 13.1 and 7.5%, respectively, by optimizing of the irrigation regime. We concluded that the low activities of key enzymes in carbon metabolism is the key limitation for the poor grain filling, as opposed to the vascular microstructures, and AWMD can increase the amount of NSC accumulation in the stems before heading, improve the utilization rate of NSCs after heading, and increase the grain filling, especially in the inferior spikelets, by altering the activities of key enzymes in carbon metabolism.

关键词： rice(Oryza sativa L.) non-structural carbohydrates(NsCs) enzymatic activity grain illing starch granules vascular bundle

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The role of the idea of mathematical modeling in advanced mathematics teaching in technical colleges

The role of the idea of mathematical modeling in advanced ma...

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第四届人文科学、管理和教育技术国际学术会议

作者： zhiqin chen Jiangxi Teachers College

Increasingly fierce competition in the society leads to higher demand for *** colleges shoulder the responsibility of training professional and skilled talents,during which process advanced mathematics teaching serves as an important *** training should be based on the training purpose and characteristics of the *** idea of mathematical modeling pays full attention to innovation and thinking differences,making it helpful in promoting students' learning initiative and advanced mathematics *** also helps to give full play to the role and value of advanced mathematics in daily life,and to promote students' abilities and comprehensive ***,certain problems exist in advanced mathematics teaching nowadays,for example,inflexible teaching methods,outdated teaching concepts,monotonous teaching methods and insufficient learning ***,the mathematical modeling idea should be introduced into the teaching of advanced mathematics,so as to fully stimulate students' interest in and efficiency of *** paper aims to explore the role of the idea of mathematical modeling in advanced mathematics teaching in technical colleges and the specific ways of *** is hoped that the advantages of modeling ideas can be leveraged to enhance its application value in advanced mathematics teaching.

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An optimized strategy of nitrogen-split application based on the leaf positional differences in chlorophyll meter readings

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Journal of Integrative Agriculture 2024年第8期23卷 2605-2617页

作者： Gaozhao Wu Xingyu chen Yuguang Zang Ying Ye Xiaoqing Qian Weiyang Zhang Hao Zhang Lijun Liu Zujian Zhang zhiqin Wang Junfei Gu Jianchang Yang Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Agricultural College Yangzhou UniversityYangzhou 225009China Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009China Key Laboratory of Arable Land Quality Monitoring and Evaluation Ministry of Agriculture and Rural AffairsYangzhou UniversityYangzhou 225127China

Modern rice production faces the dual challenges of increasing grain yields while reducing inputs of chemical ***,the disequilibrium between the nitrogen(N)supplement from the soil and the demand for N of plants is a serious obstacle to achieving these ***-based diagnosis can help farmers make better choices regarding the timing and amount of topdressing N *** objective was to evaluate a non-destructive assessment of rice N demands based on the relative SPAD value(RSPAD)due to leaf positional *** this study,two field experiments were conducted,including a field experiment of different N rates(Exp.I)and an experiment to evaluate the new strategy of nitrogen-split application based on RSPAD(***).The results showed that higher N inputs significantly increased grain yield in modern high yielding super rice,but at the expense of lower nitrogen use efficiency(NUE).The N nutrition index(NNI)can adequately differentiate situations of excessive,optimal,and insufficient N nutrition in rice,and the optimal N rate for modern high yielding rice is higher than conventional *** RSPAD is calculated as the SPAD value of the top fully expanded leaf *** value of the third leaf,which takes into account the non-uniform N distribution within a *** RSPAD can be used as an indicator for higher yield and NUE,and guide better management of N fertilizer ***,we developed a new strategy of nitrogen-split application based on RSPAD,in which the N rate was reduced by 18.7%,yield was increased by 1.7%,and the agronomic N use efficiency was increased by 27.8%,when compared with standard farmers'*** strategy of N fertilization shows great potential for ensuring high yielding and improving NUE at lower N inputs.

关键词： canopy crop management practices nitrogen nitrogen nutrition index rice SPAD

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植入前遗传学非整倍体筛查对反复种植失败患者疗效的观察

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中华生殖与避孕杂志 2022年第6期42卷 557-565页

作者：陈虹郑锦霞孙子莉伍园园陈智勤同济大学附属第一妇婴保健院生殖医学科上海201204 同济大学附属第一妇婴保健院护理部上海201204 同济大学附属第一妇婴保健院妇产科上海201204

目的探讨基于二代测序(next-generation sequencing,NGS)技术的胚胎植入前遗传学非整倍体筛查(preimplantation genetic testing for aneuploidies,PGT-A)对反复种植失败(recurrent implantation failure,RIF)患者的临床疗效。方法回顾性队列研究分析2018年12月至2020年1月期间在同济大学附属第一妇婴保健院生殖医学科接受PGT-A治疗的63例RIF患者为研究组;选取同期采用常规体外受精(in vitro fertilization,IVF)治疗的179例RIF患者为对照组,采用倾向性得分匹配的方法(propensity score matching,PSM)对女方年龄进行匹配,分析比较两组患者一般资料、促排卵结果和妊娠结局等,并采用logistic回归分析影响每移植周期累计活产率的因素。结果最终203例患者纳入分析,其中研究组61例,对照组142例,匹配了年龄差异后两组间患者的年龄、体质量指数、不孕因素、不孕年限、既往移植失败次数、基础卵泡刺激素(follicle-stimulating hormone,FSH)、窦卵泡计数和卵巢刺激方案差异均无统计学意义(均P>0.05)。研究组的无可用胚胎数比例[45.90%(28/61)]高于对照组[13.38%(19/142)],而可移植胚胎数[1(0,2)个]低于对照组[2(1,4)个],差异均有统计学意义(均P<0.001)。研究组的胚胎种植率[61.54%(24/39)]、每移植周期临床妊娠率[61.54%(24/39)]、每移植周期持续妊娠率[61.54%(24/39)]和每移植周期活产率[61.54%(24/39)]均高于对照组[27.47%(75/273),P<0.001;41.51%(66/159),P=0.024;37.11%(59/159),P=0.006和37.11%(59/159),P=0.006];但其累计活产率[39.34%(24/61)]与对照组[41.55%(59/142)]相比差异无统计学意义(P=0.770)。多因素logistics回归分析显示是否采用PGT-A治疗为影响每移植周期活产率的独立危险因素[OR(95%CI)=2.71(1.32~5.58),P=0.007],而对累计活产率没有显著影响[OR(95%CI)=2.49(0.87~7.13),P=0.089]。结论相较常规IVF治疗,NGS-PGT策略虽然能提高RIF患者的每移植周期活产率,但不会提高其累计活产率。因此,NGS-PGT策略在RIF患者中的临床应用价值仍值得商榷。

关键词：反复种植失败活产率回顾性队列研究不孕因素临床疗效二代测序

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