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Revisiting miR-155 in intervertebral disc degeneration:Blood cell signature and local cell-free profiles

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BIOCELL 2022年第2期46卷 511-517页

作者： zhiheng liu ZHEN SUN ZHONGYUAN WAN HAIQIANG WANG Department of Cardiac Surgery Xijing HospitalAir Force Medical UniversityXi’an710032China Institute of Integrative Medicine Shaanxi University of Chinese MedicineXi’an712046China Department of Orthopedics Xijing HospitalAir Force Medical UniversityXi’an710032China Department of Orthopedics Seventh Medical Center of General Hospital of PLABeijing100700China

We unraveled the expression profiles of coding-noncoding RNAs in intervertebral disc degeneration(IDD).However,it remains elusive regarding miR-155 expression in peripheral blood mononuclear cells(PBMCs)and local extracellular space in *** study aimed for investigating the miR-155 expression of PBMCs,extracellular miRNAs(ex-miRNAs)of human nucleus pulposus(NP)tissues,and morphological changes of cell death in the IDD ***,we harvested peripheral blood and NP samples from scoliosis and IDD patients as control and degenerative groups,*** standard Ficoll density-gradient centrifugation was used to isolate *** two subpopulations of PBMCs were divided based on the cellular difference in adherence,i.e.,monocytes/macrophages and *** real-time PCR was used to evaluate miR-155 relative expression in ***-miRNAs were screened by comparison between GSE19943,GSE63492,and extracellular RNA(exRNA)*** morphological changes of cell death subtypes in IDD were observed in transmission electron microscopy(TEM).Results indicate that the lower expression of miR-155 in PBMCs from IDD patients ascribed to alterations in monocytes/***,we identified 594 shared hsa-miRNAs as the intracellular miRNA expression profile and 258 miRNAs as the ex-miRNA expression profile in human NP ***-155 was amongst intracellular miRNAs in *** observation presented multiple endocytic secretory vesicles within human NP cells,implicating exosome transporting *** necroptosis and pyroptosis were noted in human ***,this study reveals miR-155 expression in PBMCs from IDD ***,we propose ex-miRNA expression profile and necroptosis/pyroptosis in human NP tissue,shedding novel light on the etiology of IDD.

关键词： miR-155 PBMCs Lumbar disc disease Auto-immunity Cell death Exosome

Low-density lipoprotein receptor-related protein 1 is a CROPs-associated receptor for Clostridioides infection toxin B

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Science China(Life Sciences) 2022年第1期65卷 107-118页

作者： Shengjie Guo Yiou Chen Jingze liu Xinyi Zhang zhiheng liu Zhuo Zhou Wensheng Wei Biomedical Pioneering Innovation Center Beijing Advanced Innovation Center for GenomicsPeking-Tsinghua Center for Life SciencesPeking University Genome Editing Research CenterState Key Laboratory of Protein and Plant Gene ResearchSchool of Life SciencesPeking UniversityBeijing 100871China

As the leading cause of worldwide hospital-acquired infection,Clostridioides difficile(***)infection has caused heavy economic and hospitalized burden,while its pathogenesis is not fully *** B(Tcd B)is one of the major virulent factors of ***,CSPG4 and FZD2 were reported to be the receptors that mediate Tcd B cellular ***,genetic ablation of genes encoding these receptors failed to completely block Tcd B entry,implicating the existence of alternative receptor(s)for this ***,by employing the CRISPR-Cas9 screen in CSPG4-deficient He La cells,we identified LDL receptor-related protein-1(LRP1)as a novel receptor for Tcd *** of LRP1 in both CSPG4-deficient He La cells and colonic epithelium Caco2 cells conferred cells with increased Tcd B resistance,while LRP1 overexpression sensitized cells to Tcd B at a low ***-immunoprecipitation assay showed that LRP1 interacts with full-length Tcd ***,CROPs domain,which is dispensable for Tcd B’s interaction with CSPG4 and FZD2,is sufficient for binding to *** such,our study provided evidence for a novel mechanism of Tcd B entry and suggested potential therapeutic targets for treating *** infection.

关键词： Clostridioides difficile low-density lipoprotein receptor-related protein 1 Tcd B toxin receptor CRISPR screening

Diversity and distribution of soil micro-fungi along an elevation gradient on the north slope of Changbai Mountain

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Journal of Forestry Research 2017年第4期28卷 831-839页

作者： Hong Yang Guozhong Lu Huiming Jiang Dong-ning Shi zhiheng liu College of Environment and Resources Dalian Nationalities UniversityDalian 116600People’s Republic of China College of Life Science Dalian Nationalities UniversityDalian 116600People’s Republic of China College of Life Science Liaoning Normal UniversityDalian 116081People’s Republic of China College of Plant Protection Shenyang Agricultural UniversityShenyang 110866People’s Republic of China

Elevational changes in patterns of diversity are important to understanding of the influence of global changes, yet few studies have addressed the distribution of microorganisms, e.g. soil micro-fungi. We studied the diversity of the forest soil micro-fungi in four vegetation belts along an elevation gradient on the north slope of Changbai Mountain in Changbai National Nature Reserve. The four belts were characterized as coniferous-deciduous mixed forest, coniferous forest, Erman's birch forest, and alpine tundra. We estimated the quantity and distribution of the fungal species in each belt and calculated three indices, viz. Shannon-Wiener diversity (H'), Pielou's evenness (J'), and Margalef's abundance (E), to depict fungal species diversity. A total of 932 strains were recorded and identified, representing 53 genera, and 108 species. Among these, Penicillium, Aspergillus, Trichoderma, Mucor, Rhizopus and Fusarium were the dominant genera. With increasing elevation, the quantity of fungi and values of H', E, and J' gradually declined.

关键词： Altitudinal gradient Distribution Diversities Micro-fungi

Optical Coherence Tomographic Features in a Case of Bilateral Macular Coloboma with Strabismus

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Eye Science 2011年第4期26卷 244-246页

作者： Shengli Hao zhiheng liu Yanshan Xu Department of Ophthalmology Tianjin Municipal Hospital of Dagang Affiliated Hospital of Tianjin Medical University School of Clinical Medicine Binhai New Area 300270 Tianjin China Clinical College of Ophthalmology Tianjin Medical University Tianjin Eye Hospital Tianjin 300020China

Purpose:To report the optical coherence tomography (OCT) findings in a patient with bilateral macular coloboma with ***:A 21-year-old male presented with macular coloboma in both eyes..Fundus photography,fundus fluorescence angiography (FFA) and OCT were ***:Color fundus photography showed a sharply-demarcated,round macular defect,approximately 4×4 disc diameters with bare sclera at the base and pigment clumping in both *** showed hypofluorescence at the macula corresponding to the size of the lesion *** revealed a crater-like depression at the macula,demonstrating atrophic neurosensory retina,and an absence of retinal pigment epithelium (RPE) and choroid in the ***:OCT can be a beneficial tool to confirm the diagnosis of macular coloboma.

关键词：光学相干层析黄斑缺损斜视 OCT 视网膜断层扫描血管造影

On The Rational Dimension of Eco-city Construction

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International English Education Research 2014年第2期 60-63页

作者： liu zhiheng SUN Daojin. Southwest University Chongqing China 400715

Reasonable ＂eco-city＂in the external between urban and rural areas should make a complete ecological cycle of the system, so that the internal energy efficiency and low pollution, resource use environmentally clean, quiet comfortable life of the people, as well as other elements of the organic： unity of urban greening. So, ＂Eco-City＂, ＂urban ecology＂ are two heterogeneous areas should be treated differently. However, in rural and urban dualistic situation, China advocated ＂ecological city＂ ignored the rural ecological balance, resulting in ＂eco-city＂development directly into＂ urban ecology＂ construction within the city along with the waste of resources, water pollution, serious problems of air pollution, noise pollution, the deep ＂eco-city＂ into representation＂ urban ecology＂, in the standard does not cover up the sound, making the generation between rural and urban＂ material Rift ,＂after all ,can not be filled.

关键词： Eco-city urban ecology sub sta_nce Rift...1

Genome-wide CRISPR activation screen identifies candidate receptors for SARS-CoV-2 entry

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Science China(Life Sciences) 2022年第4期65卷 701-717页

作者： Shiyou Zhu Ying liu Zhuo Zhou Zhiying Zhang Xia Xiao zhiheng liu Ang Chen Xiaojing Dong Feng Tian Shihua Chen Yiyuan Xu Chunhui Wang Qiheng Li Xuran Niu Qian Pan Shuo Du Junyu Xiao Jianwei Wang Wensheng Wei Biomedical Pioneering Innovation Center Beijing Advanced Innovation Center for GenomicsPeking-Tsinghua Center for Life SciencesPeking University Genome Editing Research CenterState Key Laboratory of Protein and Plant Gene ResearchSchool of Life SciencesPeking UniversityBeijing100871China State Key Laboratory of Protein and Plant Gene Research School of Life SciencesPeking UniversityPeking-Tsinghua Center for Life SciencesPeking UniversityBeijing Advanced Innovation Center for GenomicsPeking UniversityBeijing 100871China NHC Key Laboratory of Systems Biology of Pathogens and Christophe Merieux Laboratory Institute of Pathogen BiologyChinese Academy of Medical Sciences and Peking Union Medical CollegeKey Laboratory of Respiratory Disease PathogenomicsChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Academy for Advanced Interdisciplinary Studies Peking UniversityBeijing 100871China

The outbreak of coronavirus disease 2019(COVID-19) caused by SARS-CoV-2 has created a global health crisis. SARS-CoV-2 infects varieties of tissues where the known receptor ACE2 is low or almost absent, suggesting the existence of alternative viral entry pathways. Here, we performed a genome-wide barcoded-CRISPRa screen to identify novel host factors that enable SARS-CoV-2 infection. Beyond known host proteins, i.e., ACE2, TMPRSS2, and NRP1, we identified multiple host components,among which LDLRAD3, TMEM30A, and CLEC4G were confirmed as functional receptors for SARS-CoV-2. All these membrane proteins bind directly to spike’s N-terminal domain(NTD). Their essential and physiological roles have been confirmed in either neuron or liver cells. In particular, LDLRAD3 and CLEC4G mediate SARS-CoV-2 entry and infection in an ACE2-independent fashion. The identification of the novel receptors and entry mechanisms could advance our understanding of the multiorgan tropism of SARS-CoV-2, and may shed light on the development of COVID-19 countermeasures.

关键词： CRISPRa screen SARS-CoV-2 novel receptors

基于局部最优点的GNSS分布式压制干扰源部署算法

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兵工学报 2024年第9期45卷 3240-3252页

作者：刘志衡刘伟平焦博战略支援部队信息工程大学地理空间信息学院河南郑州450001

为实现对特定区域卫星导航信号进行有效管制,通常可以使用压制干扰源对全球卫星导航系统(Global Navigation Satellite System,GNSS)实施导航干扰,而GNSS干扰源的部署方案将直接决定能否取得理想干扰效果。以单GNSS干扰源可视域分析方... 详细信息

为实现对特定区域卫星导航信号进行有效管制,通常可以使用压制干扰源对全球卫星导航系统(Global Navigation Satellite System,GNSS)实施导航干扰,而GNSS干扰源的部署方案将直接决定能否取得理想干扰效果。以单GNSS干扰源可视域分析方法为基础,研究GNSS干扰源分布式部署算法,在对传统排列组合算法分析的基础上提出计算效率更高的全局搜索算法。当任务区域增大时,为了进一步提高算法效率,在全局搜索算法的基础上又引入局部最优点的概念,提出一种基于局部最优点的优化搜索算法,且当局部最优点等级为0阶时,全局搜索算法与基于局部最优点的优化搜索算法互为等价,从而保证两种算法的数学统一。实验分析结果表明:当任务区域较小时,与传统排列组合算法相比,采用全局搜索算法能够在结果差异不大的情况下将计算效率提升99%以上;当任务区域较大时,与全局搜索算法相比,采用不同等级局部最优点的优化搜索算法,能够进一步将计算效率提升60%~97%。

关键词：卫星导航局部最优点分布式压制干扰地形遮挡可视域分析

Design of an all-position intelligent welding robot system

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Design of an all-position intelligent welding robot system

Integrative Analysis of Genome,3D Genome,and Transcriptome Alterations of Clinical Lung Cancer Samples

第32届中国控制与决策会议

作者： Xin Deng zhiheng liu Ruifeng Li Harbin Institute of Technology

In order to reduce human intervention in the welding process and improve the intellectualization of the welding robot,a kind of all-position intelligent welding robot is designed in this paper,which improves the dynamic performance of the robot through the optimization of mechanical structure and driving system,and establishes a multiinformation fusion laser welding *** data management expert system can process the time-sharing and distribution of information and realize the comprehensive *** laser ranging sensor is used to track the weld seam in real-time.A robust control algorithm based on joint flexibility is proposed for the nonlinear factors of friction,hysteresis and clearance in the transmission *** image of the special-shaped pipe is acquired by a high-speed camera,and the target welded parts are identified and located by the deep learning neural network,so as to adjust the welding parameters and support the control system to track the target.

关键词： Welding robot System Design Seam tracking Machine learning Intelligent welding

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Genomics, Proteomics & Bioinformatics 2021年第5期19卷 741-753页

作者： Tingting Li Ruifeng Li Xuan Dong Lin Shi Miao Lin Ting Peng Pengze Wu Yuting liu Xiaoting Li Xuheng He Xu Han Bin Kang Yinan Wang zhiheng liu Qing Chen Yue Shen Mingxiang Feng Xiangdong Wang Duojiao Wu Jian Wang Cheng Li Center for Bioinformatics School of Life SciencesCenter for Statistical SciencePeking UniversityBeijing 100871China BGI-Shenzhen Shenzhen 518083China Zhongshan Hospital Institute of Clinical Science Fudan UniversityShanghai Institute of Clinical BioinformaticsShanghai 200433China Fudan University Center for Clinical Bioinformatics Shanghai 200433China Department of Thoracic Surgery Zhongshan Hospital of Fudan UniversityShanghai 200032China School of Life Sciences Tsinghua UniversityBeijing 100084China BGI-Qingdao Qingdao 266426China Shenzhen Engineering Laboratory for Innovative Molecular Diagnostics BGI-ShenzhenShenzhen 518083China iCarbonX Shenzhen 518053China Digital Life Research Institute Shenzhen 518110China State Key Laboratory of Proteomics National Center of Biomedical AnalysisInstitute of Basic Medical SciencesBeijing 100850China China National GeneBank BGI-ShenzhenShenzhen 518083China

Genomic studies of cancer cell alterations,such as mutations,copy number variations(CNVs),and translocations,greatly promote our understanding of the genesis and development of ***,the 3D genome architecture of cancers remains less studied due to the complexity of cancer genomes and technical *** explore the 3D genome structure in clinical lung cancer,we performed Hi-C experiments using paired normal and tumor cells harvested from patients with lung cancer,combining with RNA sequenceing *** demonstrated the feasibility of studying 3D genome of clinical lung cancer samples with a small number of cells(1×10^(4)),compared the genome architecture between clinical samples and cell lines of lung cancer,and identified conserved and changed spatial chromatin structures between normal and cancer *** also showed that Hi-C data can be used to infer CNVs and point mutations in *** integrating those different types of cancer alterations,we showed significant associations between CNVs,3D genome,and gene *** propose that 3D genome mediates the effects of cancer genomic alterations on gene expression through altering regulatory chromatin *** study highlights the importance of analyzing 3D genomes of clinical cancer samples in addition to cancer cell lines and provides an integrative genomic analysis pipeline for future larger-scale studies in lung cancer and other cancers.

关键词： Lung cancer 3D genome Copy number variation Clinical sample Integrative genomic analysis